20150316 - qPCR H.discus H2O and feces

	A	B	C	D	E	F
1	Master Mix Calcs
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5	Withering Syndrome
6		Ci	Cf	Vol. (uL)	# Rxns	Total Vol (uL)
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9	Promega 2x GoTaq Probe Master Mix	2	1	12.5	33	412.5
10	WSN1 F	10	0.32	0.8	33	26.4
11	WSN1 R	10	0.32	0.8	33	26.4
12	RLP_P (ABI)	10	0.2	0.5	33	16.5
13	BSA	20	0.60	0.75	33	24.75
14	H2O			9.65	33	318.45
15	Total			25		Add 23uL to each well
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18	Phage
19		Ci	Cf	Vol. (uL)	# Rxns	Total Vol (uL)
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21	Template			2
22	BioRad 2x Sso Fast Eva Green Master Mix	2	31.25	12.5	16	200
23	Pf	10	6.25	0.5	16	8
24	Pr	10	6.25	0.5	16	8
25	H2O			9.5	16	152
26	Total			25		Add 23uL to each well
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