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Studier FW (2005), Protein Production by Auto-Induction in High-Density Shaking Cultures. Protein Expr. Purif. 41(1): 207–234. <-- making ZYM-5052.
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Practical recap of Studier 2005 by Studier:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771296/pdf/pro0018-0936.pdf
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Stable Expression Clones and Auto-Induction for Protein Production in E. coli (Studier 2014)
http://www.ncbi.nlm.nih.gov/pubmed/24203322
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Grabski A, Mehler M, Drott D (2005), The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep. Nature Methods 2, 233 – 235.
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Imperial college summary:
http://www3.imperial.ac.uk/pls/portallive/docs/1/15699698.PPT
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potentially fancier (trace elements used): http://www.helmholtz-muenchen.de/fileadmin/PEPF/PDF/Studier_media.pdf
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Base Broth
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2desired L
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12g Na2HPO4 (heptahydrate)*
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6g KH2PO4 (anhydrous)*
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40g Tryptone
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10g Yeast Extract
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10g NaCl
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autoclave
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Sugar mix
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1desired L
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150mL glycerol
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12.5g glucose
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50g lactose
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filter sterilize
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add 40mL per L of base
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Contact: Janet Matsen (janetmatsen@gmail.com, jmatsen@uw.edu)
* Eric Mukherjee 7/2016: It seems that if you assume they mean the heptahydrate for the Na2HPO4 and anhydrous KH2PO4, then you get 22mM of both KH2PO4. It looks like the protocol calls for 25mM of each of those. So it looks like it should be 6.8045g of KH2PO4 anhydrous in 2L and 13.4035 g Na2HPO4 heptahydrate in 2L total if you want 25mM (feel free to double check this).
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Comparison to IPTG induction:
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https://docs.google.com/document/d/1S8ezz63qweLv_m3zplrgjoMd5cemiAaTYPyQRx_zBA0/edit
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