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Week 1 : Wet-Lab
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WEEK 1Monday
5.13.24		DAY 1:
Welcome, Training Overview & Journal Club		Start timeTopicDetailsLocation (4th floor of WBS Building)Instructors
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8.00 AM – 8.30 AMArrival, registration and breakfast4004MMC Staff
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8.30 AM – 9.00 AMWelcome and Introduction4004Maira
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9.00 AM – 10:00 AMIntroduction to mNGS & Training Overview 4004Gowtham
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10.00 AM – 11:00 AM
mNGS in the Context of Infectious Disease
4004Natasha
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11.00 AM – 12.00 AM Journal Club #1: Clinical mNGS ReviewJournal #14004Juliana
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12.00 AM – 1.00 PMLunch4004
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1.00 PM – 4.00 PMIndividual SPRI Bead Practice*SPRI Bead Theory and Practice

4003Daniele
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Tuesday
5.14.24		DAY 2: RNA mNGS Lab IStart timeTopicDetailsLocationInstructors
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8.00 AM – 8.30 AMArrival, registration and breakfast4004
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8.30 AM – 10.30 AMFrom Sample to Sequences: An Overview*Sample types
*TNA extraction methods & QC- Theory
*Illumina library preparation
*Iibrary QC & Sequencing
*RNA QC demo		4004Juliana & Gowtham
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10.30 AM – 11.00 AMCase and Sample Set Presentation*Training Sample Set4004Juliana
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11.00 AM – 12.00 PMLunch4004
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12.00 PM – 4.00 PMIllumina RNA Library Preparation *Fragmentation + priming
*First strand synthesis
*Second strand synthesis
*SPRI clean-up		4003Juliana, Gowtham, Daniele
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Wednesday 5.15.24Day 3: RNA mNGS Lab IIStart timeTopicDetailsLocationInstructors
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8.00 AM – 8.30 AMArrival, registration and breakfast4004
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8.30 AM - 12:00 PMIllumina RNA Library Prep (cont.) *End-prep
*Adapter ligation
*SPRI clean-up and size selection
*USER + barcoding PCR
*SPRI clean-up and size selection		4003Juliana, Gowtham, Daniele
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12.00 PM - 1.00 PMLunch4004
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1.00 PM – 1.30 PMIllumina library QC theory4004Juliana, Gowtham, Daniele
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1.30 PM – 4.00 PMIllumina RNA Library QC & Pooling*SPRI clean-up and size selection
*Library QC
*Library pooling		4003Juliana, Gowtham, Daniele
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Thursday 5.16.24Day 4: Illumina SequencingStart timeTopicDetailsLocationInstructors
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8.00 AM – 8.30 AMArrival, registration and breakfast4004
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8.30 AM – 9.30 AMIllumina sequencing theory4004Juliana
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9:30 AM - 12.00 PMQC Buffer time / Review session4004Juliana
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12.00 PM - 1.00 PMLunch4004
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1.00 PM - 2:30 PMiSeq loading *Pool dilution
*iSeq loading
4003Juliana
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2.30 PM - 4.00 PM Journal Club #2: mNGS in surveillance in BangladeshJournal Club #24004Daniele
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Friday
5.17.24		Day 5: BaseSpace / Sequencing resultsStart timeTopicDetailsLocationInstructors
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8.00 AM – 8.30 AMArrival, registration and breakfast4004
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9.00 AM – 10.00 AMReview session4004Juliana, Gowtham, Daniele, Natasha
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10.00 AM - 12.00 AMJournal Club #3: mNGS in meningitis clinical cases- breakout groups to discuss each caseJournal Club #34004Gowtham
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12.00 PM - 1.00PMLunch4004
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1.00PM-3.00PMBasespace and sequencing quality control4003 Gowtham
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