Rawls Glycerol Stock Collection
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TimestampBox #Stock #OrganismParent strainStrain namePlasmid nameAntibiotic resistanceKeywordsDate frozenDescription ReferenceGenotypeConstruct byPlasmid MapInsert digital plasmid maps as a link
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11Pseudomonas aeruginosaPAKPAK1/5/2007wt strain provided by Andrew Goodman and Steven Lory in 2006 (D. Bradley strain)
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110Listeria monocytogenesEGDpNF8Erm101/5/2007Grow on BHI or LB at 37C aerobic
pNF8 constitutively expresses GFP (permission received from Dr. Nicholas Fortineau to use this plasmid)
from Taiki Aoshi and Mark Miller (Wash. U.)Dr. Nicholas FortineauNo
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111Escherichia coliTOP10pRZT3Tet1/5/2007Broad host range plasmid, DsRed expressed from lac promoter
Orientation of DsRed not checked
from Wilbert BitterYes
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112Escherichia coliXL-1 BluepMP4518-YFPGm1/5/2007Broad host-range plasmid; EYFP expressed from lac promoterStuurman et al. (2000) MPMI 13: 1163-1169Nico StuurmanNo
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113Escherichia coliDH5apCS-TPAmp1001/5/2007contains Tol2 transposase cDNAKoichi KawakamiYes
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114Lactococcus lactisIL1403JIM4886pJIM2289Erm1/5/2007pJIM2289 is a modified version of pJIM2366 that contains PhisD3 upstream from the luxAB gene.

Corthier G, Delorme C, Ehrlich S D, Renault P. Use of luciferase genes as biosensors to study bacterial physiology in the DT. Appl Environ Microbiol. 1998;64:2721–2722.No
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115Escherichia coliMG1655pMF230-CFPAmp100Carb301/5/2007Attempt to replace GFP with CFP in pMF230 (CFP expressed by trc promoter)
Successful insertion of CFP cassette may not have been confirmed for this clone.
Michael MahowaldNo
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116Pseudomonas alcaligenesLMG12241/5/2007
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117Escherichia coliTOP10pMF230Amp100Carb301/5/2007pMF36 with mut2 GFP expressed from trc promoterNivens et al. (2001) "Role of Alginate and Its O Acetylation in Formation of Pseudomonas aeruginosa Microcolonies and Biofilms" J Bacteriol. 183(3): 1047–1057.No
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118Escherichia coliMG1655pRZT3Tet1/5/2007Broad host range plasmid, DsRed expressed from lac promoter
Orientation of DsRed not checked
from Wilbert Bitter
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119Escherichia coliXL-1 BluepMP45181/5/2007Broad host-range plasmid; EYFP expressed from lac promoterStuurman et al. (2000) MPMI 13: 1163-1169Nico StuurmanNo
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12Pseudomonas aeruginosaPA141/5/2007wt strain provided by Andrew Goodman and Steven Lory in 2006
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120Pseudomonas aeruginosaPAKPAK delta-retSretS1/5/2007retS deletion strainGoodman et al., 2004
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121Pseudomonas aeruginosaPAKPAK delta-fliCfliC1/5/2007fliC deletion strainDasgupta et al., 2003
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122Pseudomonas aeruginosaPAKPAK delta-mexABmexAB1/5/2007In-frame nonpolar deletion of mexABMatt Wolfgang
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123Pseudomonas aeruginosaPAKPAK delta-lasR delta-rhlRlasR rhlR1/5/2007lasR and rhlR deletion strainAndy Goodman / Matt Wolfgang
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124Pseudomonas aeruginosaPAKPAK delta-lasI delta-rhlIlasI rhlI1/5/2007lasR
Andy Goodman / Matt Wolfgang
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125Pseudomonas aeruginosaPAKPAK delta-pilApilA1/5/2007pilA deletion strainKagami et al., 1998
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126CellulomonasTSFN1AE.A3T1E1A031/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S sequencing identifies this clone as Cellulomonas (Actinobacteria), closest cultured relative AY655729.1 Cellulomonas parahominis strain W7385
Rawls et al., 2006
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127AeromonasTSFN1AE.B7T1E1B071/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days
16S rRNA sequencing shows this to be Aeromonas (Gamma-proteobacteria), with closest cultured relative AF099027 Aeromonas sp. strain T8
Rawls et al., 2006
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128StaphylococcusMRZN2AE.A4M2E1A041/5/2007Cultured from intestine of 10dpf zebrafish colonized since 3dpf with conventional mouse cecal microbiota
16S rRNA sequencing shows this to be Staphylococcus (Bacilli), with closest cultured relative AB219154.1 Staphylococcus lentus strain:SSH39
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129StaphylococcusTSFN1AE.D101/5/2007Origin unknown (errantly picked from glycerol plate in search of a different clone described in Rawls et al., 2006)
16S rRNA sequencing shows this to be Staphylococcus (Bacilli), with closest cultured relative AF532917.1 Staphylococcus pasteuri strain ZA-b3
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13Pseudomonas aeruginosaPAO11/5/2007wt strain provided by Steven Lory in 2003 (M. Vasil strain)
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130AeromonasTSFN1AE.H7T1E1H071/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days
16S rRNA sequencing shows this to be Aeromonas (Gamma-proteobacteria) with closest relative AY689069.1 Aeromonas sp. 9C_12
Rawls et al., 2006
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131Plesiomonas1/5/2007Origin unclear (errantly picked from glycerol plates in search of a different clone from Rawls et al., 2006)
16S rRNA sequencing shows this to be Plesiomonas, with closest relative PS16SRDN Plesiomonas shigelloides strain NCIMB9242

This clone IDed during glylcerol plate recovery attempt for clone ZFMB1AE.D1
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132PseudomonasZFMB1AE.E7ZF1EE071/5/2007Cultured from intestine of adult conventionally-raised zebrafish.
16S rRNA sequencing shows this to be Pseudomonas (Gamma-proteobacteria), with closest relative AY880303.1 Pseudomonas pseudoalcaligenes strain M5-4
Rawls et al., 2006
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133EnterococcusMRZN2AE.F6M2E1F061/5/2007Cultured from intestine of 10dpf zebrafish colonized since 3dpf with conventional mouse cecal microbiota
16S rRNA sequencing shows this to be Enterococcus (Bacilli ), with closest relatives (tied with several others) AY850358.1 Enterococcus faecalis strain SFL
Rawls et al., 2006
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134ProteusTSFNXAE.F121/5/2007Origin unclear (errantly picked from glycerol plates in search of a different clone from Rawls et al., 2006)
16S rRNA sequencing shows this to be Proteus, with closest relative AF008582 Proteus mirabilis
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135ProteusMRZN2AE.G2M2E1G021/5/2007Cultured from intestine of 10dpf zebrafish colonized since 3dpf with conventional mouse cecal microbiota.
16S rRNA sequencing shows this to be Proteus (Gamma-proteobacteria), with closest relative AF008582 Proteus mirabilis
Rawls et al., 2006
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136AeromonasTSFN1AE.A6T1E1A061/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S rRNA sequencing shows this to be Aeromonas (Gamma-proteobacteria), with closest relative AF099027 Aeromonas sp. strain T8
Rawls et al., 2006
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137ExiguobacteriumZFMB1AE.B2ZF1EB021/5/2007Cultured from intestine of adult conventionally-raised zebrafish.
16S rRNA sequencing shows this to be Exiguobacterium (Bacilli), with closest relative DQ083948.1 Exiguobacterium sp. MY02
Rawls et al., 2006
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138Pseudomonas aeruginosaPAKPAK delta-exsA1/5/2007exsA deletion strainWolfgang et al., 2003
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139EnterococcusMRZN2AE.D3M2E1D031/5/2007Cultured from intestine of 10dpf zebrafish colonized since 3dpf with conventional mouse cecal microbiota.
16S rRNA sequencing shows this to be Enterococcus (Bacilli ), with closest relative EGA301833 Enterococcus gallinarum strain LMG 13129.
Rawls et al., 2006
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14Aeromonas hydrophilaATCC 356541/5/2007
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140AeromonasTSFN1AE.A7T1E1A071/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S rRNA sequencing shows this to be Aeromonas (Gamma-proteobacteria), with closest relative AF099027 Aeromonas sp. strain T8
Rawls et al., 2006
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141TSFN1AN.E5T1N1E051/5/2007Cultured anaerobically from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S rRNA sequencing shows this to be related to Carnobacterium (Bacilli), with closest relatives (97%id) DIN17300 Desemzia incerta, followed very closely by BIY14650 Brevibacterium incertum.
Rawls et al., 2006
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142ShewanellaTSFN1AE.C5T1E1C051/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S rRNA sequencing shows this to be Shewanella (Gamma-proteobacteria), with closest relative AJ609571.1 Shewanella decolorationis, type strain CCTCC M 203093T.
Rawls et al., 2006
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143CitrobacterTSFN1AE.C7T1E1C071/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S rRNA sequencing shows this to be Citrobacter (Gamma-proteobacteria), with closest relative AB210978.1 Citrobacter freundii, strain SSCT56
Rawls et al., 2006
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144EnterococcusMRZN2AE.D5M2E1D051/5/2007Cultured from intestine of 10dpf zebrafish colonized since 3dpf with conventional mouse cecal microbiota.
16S rRNA sequencing shows this to be Enterococcus (Bacilli), with closest relative(tied with several other hits) AF039903 Enterococcus casseliflavus
Rawls et al., 2006
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145ClostridiumTSFN1AN.G10T1N1G101/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S rRNA sequence shows this to be Clostridium (Clostridia), with closest relative(tied with several other hits) AY781385.1 Clostridium intestinale
Rawls et al., 2006
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146PlesiomonasTSFN1AN.D3T1N1D031/5/2007Cultured from cecum of adult mouse colonized with conventional zebrafish gut microbiota for 14 days.
16S rRNA sequencing shows this to be Plesiomonas (Gamma-proteobacteria), with closest relative PS16SRDN Plesiomonas shigelloides, strain NCIMB9242
Rawls et al., 2006
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147AeromonasZFMB1AE.F51/5/2007Origin unclear (errantly picked from glycerol plates in search of a different clone from Rawls et al., 2006).
16S rRNA seqeuncing shows this to be Aeromonas, with closest relative AJ16SRRNA Aeromonas jandaei (ATCC 49568)
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148PseudomonasZFMB1AE.E7ZF1EE071/5/2007Cultured from intestine of adult conventionally-raised zebrafish.
16S rRNA sequencing shows this to be Pseudomonas (Gamma-proteobacteria), with closest relative AY880303.1 Pseudomonas pseudoalcaligenes strain M5-4
Rawls et al., 2006
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149CitrobacterTSFN1AN.F81/5/2007Origin unclear (errantly picked from glycerol plates in search of a different clone from Rawls et al., 2006).
16S rRNA sequencing shows this to be Citrobacter, with closest relative DQ010114.1 Citrobacter freundii strain HQ010516B-1
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15Pseudomonas alcaligenesATCC 149091/5/2007
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1501/5/2007empty?
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151Escherichia coliDH5aTol2 GW Cfos (Clone 1)Amp1001/9/2007Fisher et al., 2006E. GrierYes
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152Pseudomonas aeruginosaPAKPAKpSMC21 (GFP)Amp100Carb150Kan501/9/2007Wolfgang Lab #03-257
pSMC21: GFPmut2 under the control of the constitutive lac promoter; AmpR, CbR, KanR
Bloemberg et al., 1997No
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153Pseudomonas aeruginosaPAKPAK delta-FliC1/9/2007fliC deletion strainDasgupta et al., 2003
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154Escherichia coliMG16551/9/2007wt strain
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155Pseudomonas aeruginosaPAO11/9/2007
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156Pseudomonas aeruginosaPA141/9/2007
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157Escherichia coliTOP1012C-I1/9/2007Tol2GW-Cfos w/ Fiaf3.5kb inserted in GW cloning site with TdTomato exchanged for GFPChad Trent
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158Escherichia coliDH5aFiaf.In1 BP 11/10/2007Confirmed by sequencing - used for fiaf intron1 LR reaction
Fiaf intron 1 cloned into pDONR221 using Invitrogen Gateway system
Chad Trent
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159Escherichia coliDH5aFiaf.In1 BP 21/10/2007Confirmed by sequencing
Fiaf intron 1 cloned into pDONR221 using Invitrogen Gateway system
Chad Trent
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16Shewanella putreficiensATCC 80711/5/2007
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160Escherichia coliDB3.1Fiaf LR 1 - Tol2GW Cfos1/10/2007Confirmed by sequencing
Zebrafish fiaf intron 1 inserted into Tol2GWcfos vector using Invitrogen Gateway system (clone 1)

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161Escherichia coliDB3.1Fiaf LR 2 - Tol2 GW Cfos1/10/2007Unconfirmed by sequencing

Zebrafish fiaf intron 1 inserted into Tol2GWcfos vector using Invitrogen Gateway system (clone 2)
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162Pseudomonas aeruginosa
PAO1cml-31/25/2007from Mike Vasil (2nd clone in John's box)
Parent strain for delta-pvdS strain
Molecular Microbiology (1996), 21(5) p.1019-1028
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163Pseudomonas aeruginosaPAO1 (cml-3)delta-pvdSGm501/25/2007pvdS deletion strain from Mike Vasil (second clone in John's Box); pvdS repalced with GmR cassetteMolecular Microbiology (1996), 21(5) p.1019-1028
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164Escherichia coliDH5afn04b02 (Dr saa) Amp1001/25/2007pT7T3D-Pac vector
Zebrafish EST encoding saa, Clone #1
Antisense: XhoI or AvaI; T3
Sense: PvuII; T7
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165Escherichia coliDH5afaa70a11 (Dr pcna)Amp1001/25/2007pBluescript SK- vector
Zebrafish EST encoding pcna, Clone #1
Anitsense: BamHI or NotI; T7
Sense: KpnI; T3
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166Escherichia coliDH5afp17e05 (Dr mpo/mpx)Kan501/25/2007pBK-CMV vector
Zebrafish EST encoding mpo/mpx, Clone #1
Antisense: ???
Sense: ???
65
167Escherichia coliDH5afs32b01 (Dr gpx2)Amp1001/25/2007pSPORT1 vector
Zebrafish EST encoding gpx2, Clone #1
Antisense: EcoRI or KpnI; Sp6
Sense: XbaI, BamHI, or HindIII; T7
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168Escherichia coliDH5afo75e06 (Dr mpo/mpx)1/25/2007pBK-CMV vector
Zebrafish EST encoding mpo/mpx, Clone #1
Antisense: ???
Sense: ???
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169Escherichia coliDH5aUAS M-YFPKan501/25/200714X UAS driving expression of membrane tagged YFP
Clone 3
Eric Schroeter and Jeff Mumm (2004)
68
17Lactococcus lactisIL14031/5/2007from Alexei Sorokin (Jouy en Josas, France)
69
170Escherichia coliDH5aUAS M-CFPKan501/25/200714X UAS driving expression of membrane tagged CFP
Clone 3
Eric Schroeter and Jeff Mumm (2004)
70
171Escherichia coliDH5aUAS MSC-YFPKan501/25/200714X UAS driving expression of YFP
Clone 3
Mike Nonet (2004)
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172Escherichia coliDH5apTM100TetCm1/25/2007Aeromonas expression vector (Clone 1)
Mobilizable plasmid, contains additional SalI sites and SmaI sites, and has lost an SstII site.
Yu Hongbing and Leung Ka YinYes
72
173Escherichia coliDH5aTol2 MCS eGFPAmp1001/25/2007Tol2 vector containing a multiple cloning site 5' to eGFP
Clone 1
Maia Dorsett and Steve JohnsonYes
73
174Escherichia coliDH5aTol2 MCS Amp1001/25/2007Tol2 vector containing a multiple cloning site
Clone 1
Maia Dorsett and Steve JohnsonYes
74
175Escherichia coliDH5aBAC C137E23Cm301/25/2007Clone 1
Zebrafish BAC containing fiaf locus; from CHORI211 library; BACPAC2.1 vector
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176Pseudomonas aeruginosaPAKPAK


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177Escherichia coliDH5apBK-CMV zMPO #1Kan501/25/2007pBK-CMV vector
Zebrafish mpo clone from Jennifer Rhodes (Clone 1)
Antisense: SalI; T7
Sense: NotI; T3
Jennifer Rhodes and Tom LookeNo
77
178Escherichia coliDH5afiaf
Amp1001/25/2007Yes
78
179Escherichia coliDB3.11/25/2007Overexpression mutant strain for ccdB-containing plasmids
79
18Pseudomonas aeruginosaPAO11/5/2007wt strain (Lausanne)
parent strain for PAO6261
from Sorana Crivii (Lausanne, France)
80
180Escherichia coliccdB survival strain1/25/2007for ccdB-containing plasmids
81
181PseudomonasKG 3dpf-81/25/2007Putative Pseudomonas strain from Karen Guillemin; their 16S analysis suggests that this is related to Pseudomonas pseudoalcaligenes
Grow in TSB/TSA
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182Pseudomonas4dpf-151/25/2007Putative Pseudomonas strain from Karen Guillemin; their 16S analysis suggests that this is related to Pseudomonas putida
Grow in TSB/TSA
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183KG unidentified1/25/2007Putative Pseudomonas strain from Karen Guillemin; no sequence data, grown on Pseudomonas selective agar.
Grow in TSB/TSA
84
184ChryseobacteriumKG 3dpf.11/25/2007from Karen Guillemin; their 16S analysis suggests that this is related to Chryseobacterium sp.
Grow at 30C or RT in TSB/TSA
85
185FlavobacteriumKG 3dpf-121/25/2007from Karen Guillemin; ; their 16S analysis suggests that this is related to Uncultured Flavobacterium.
Grow at 30C or RT in TSB/TSA; grows slowly
86
186Pseudomonas aeruginosaPAKPAK delta-FliCpSMC21 (GFP)Amp100Carb150Kan501/25/2007Wolfgang Lab #06-048
pSMC21: GFPmut2 under the control of the constitutive lac promoter; AmpR, CbR, KanR
Bloemberg et al., 1997
87
187Escherichia coliDH5apDONR221-fiaf3.5kb clone 121/25/2007BP clone used for transgenic fiaf (3.5 kb)
88
188Escherichia coliDH5apDONR221 fiaf7kb clone 191/25/2007BP clone used for transgenic fiaf (7kb construct)
89
189Bacteroidetes thetaiotaomnicron54821/25/2007
90
19Pseudomonas aeruginosaPAO1PAO6261PAO6261, anr1/5/2007PAO1 delta-anr
Zrc- Nar- Hcn-
from Sorana Crivii (Lausanne, France); they obtained this strain from Axel Zimmerman; Ye et al. (1995) J. Bact. 177: 3606-3609
91
190Bacteroidetes vulgatus1/25/2007
92
191Bacteroidetes distiastonis1/25/2007
93
192Escherichia coliDH5aDr lpl1 EST #1Amp100Kan501/25/2007pCR2.1 vector
Zebrafish cDNA for lpl1 (NM_131127), clone #1
Antisense: HindIII,KpnI,SacI; T7
Sense: XbaI,XhoI,NotI; ???
94
193Escherichia coliDH5aDr cebpa EST #1Amp100Kan501/25/2007pCR2.1 vector
Zebrafish cDNA for cebpa (NM_131885), clone #1
Antisense: BamHI,HindIII,KpnI; T7
Sense: XbaI,XhoI,NotI; ???
95
194Escherichia coliDH5aDr pparg EST #1Amp100Kan501/25/2007pCR2.1 vector
Zebrafish cDNA for pparg (XM_680013), clone #22-1
Antisense: BamHI,HindIII,KpnI,SacI; T7
Sense: XbaI,XhoI,NotI; ???
96
195Pseudomonas aeruginosaPAK1/25/2007wt PAK strain from Dieter Jendrossek (clone 1)
Parent strain for ins atuR, ins liuC, and ins atuD
Forster-Fromme et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2006, p. 4819–4828
97
196Pseudomonas aeruginosaPAKins atuRGm501/25/2007Insertional mutant in atuR, from D. Jendrossek (clone 1)Forster-Fromme et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2006, p. 4819–4828
98
197Pseudomonas aeruginosaPAKins liuCGm501/25/2007Insertional mutant in liuC, from D. Jendrossek (clone 1)Forster-Fromme et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2006, p. 4819–4828
99
198Pseudomonas aeruginosaPAKins atuDGm501/25/2007Insertional mutant in atuD, from D. Jendrossek (clone 1)Forster-Fromme et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2006, p. 4819–4828
100
199Escherichia coliTOP10DrAdd1 EST pCR4Amp100Kan502/2/2007Dr add1 EST
vector pCR4, CMT clone 1
EST for zebrafish Srebf1/Srebp1c/Add1 homolog
Antisense: NotI; T3
Sense: SpeI, PmeI; T7
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