ABCDEFGHIJKLMNOPQAAABACADAEAFAGALAMANAOAPAQARASATAUAVAWAXAYAZBA
1
sample_datestar_IDstar_sizetreatment_grp
experiment_day
disease_signcoelom_IDextraction_datekitelution_liquidelution_vol_ulqubit_run_dateul_run_on_qubitqubit_std_1qubit_std_2RNA_readoutunitsRNA_2unitsfinal_vol_ultotal-RNA_ng
morethan_250ng_RNA_yes_no
morethan_100ng_total_RNA_yes_no
conc_original_qubt_10ngul_t_o_f
send_for_seq_yes-or-no
notes
RNA_-080C_yes_no
notes_on_star_at_sampling_time
86
202206216largeexposed0healthyPSC 00852023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.379.92ng/ul14138.88FALSETRUEFALSE
87
2022062129largecontrol0healthyPSC 00862023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.3719.7ng/ul14275.8TRUETRUETRUE
88
2022062131largecontrol0healthyPSC 00872023-03-09
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-09182.141565.3220.4ng/ul14285.6TRUETRUETRUE
treated cells like blood and did modified protocol
89
2022062111largeexposed0healthyPSC 00882023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.375.64ng/ul1478.96FALSEFALSEFALSE
90
2022062118largeexposed0healthyPSC 00892023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.375.22ng/ul1473.08FALSEFALSEFALSE
91
2022062127largecontrol0healthyPSC 00902023-03-09
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-09182.141565.329.62ng/ul14134.68FALSETRUEFALSE
treated cells like blood and did modified protocol
92
2022062119largeexposed0healthyPSC 00912023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.379.66ng/ul14135.24FALSETRUEFALSE
93
2022062126largecontrol0PSC 009200FALSEFALSEFALSE
94
2022062113largeexposed0healthyPSC 00932023-03-09
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-09182.141565.3284.8ng/ul141187.2TRUETRUETRUE
treated cells like blood and did modified protocol
95
2022062133largecontrol0healthyPSC 00942023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.379.38ng/ul14131.32FALSETRUEFALSE
96
2022062110largeexposed0healthyPSC 00952023-03-09
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-09182.141565.3270.2ng/ul14982.8TRUETRUETRUE
treated cells like blood and did modified protocol
97
202206214largecontrol0healthyPSC 00962023-03-29
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-291NANANAng/ul78.6ng/ul131021.8TRUETRUETRUE
removed 1ul for qubit, but messed up math, so will have to re-do qubit
arms twisted - 7
98
2022062112largeexposed0PSC 009700FALSEFALSEFALSE
99
2022062132largecontrol0PSC 009800FALSEFALSEFALSE
100
2022062128largeexposed0healthyPSC 00992023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.3726.2ng/ul14366.8TRUETRUETRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion
101
2022062114largecontrol0PSC 01002023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.376.82ng/ul1495.48FALSEFALSEFALSE
accidentally pipetted to mix DNase treatment instead of gentle inversion
102
2022062130largeexposed0PSC 010100FALSEFALSEFALSE
103
2022062115largecontrol0PSC 010200FALSEFALSEFALSE
104
2022062122largeexposed0PSC 010300FALSEFALSEFALSE
105
202206213largeexposed0PSC 010400FALSEFALSEFALSE
106
2022062123largecontrol0healthyPSC 01052023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.3743.5ng/ul14609TRUETRUETRUE
after removal of RNAlater supernatant, the pellet was smaller and not white like the others
uneaten mussels
107
202206215largeexposed0PSC 010600FALSEFALSEFALSE
108
202206217largecontrol0PSC 010700FALSEFALSEFALSE
109
2022062120largecontrol0PSC 010800FALSEFALSEFALSE
110
2022062117largecontrol0PSC 010900FALSEFALSEFALSE
111
2022062125largeexposed0PSC 011000FALSEFALSEFALSE
112
202206218largecontrol0healthyPSC 01112023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.377.38ng/ul14103.32FALSETRUEFALSE
step 1 of purification --> had to spin 4x to get all sample through yellow filter, was clogging it
113
202206219largecontrol0PSC 011200FALSEFALSEFALSE
114
2022062121largeexposed0PSC 01132023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.376.86ng/ul1496.04FALSEFALSEFALSE
accidentally pipetted to mix DNase treatment instead of gentle inversion
stretched
115
2022062146smallexposed0healthyPSC 01142023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.375.42ng/ul1475.88FALSEFALSEFALSE
116
2022062150smallexposed0healthyPSC 01152023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.37TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
117
2022062138smallexposed0healthyPSC 01162023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.37TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
118
2022062148smallexposed0healthyPSC 01172023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.37TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
119
2022062160smallcontrol0healthyPSC 01182023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.3710.9ng/ul14152.6FALSETRUETRUE
120
2022062163smallcontrol0healthyPSC 01192023-03-29
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-291NANANAng/ul81.6ng/ul131060.8TRUETRUETRUE
removed 1ul for qubit, but messed up math, so will have to re-do qubit
121
2022062162smallcontrol0PSC 012000FALSEFALSEFALSE
122
2022062145smallcontrol0PSC 012100FALSEFALSEFALSE
123
2022062149smallcontrol0PSC 012200FALSEFALSEFALSE
124
2022062151smallcontrol0healthyPSC 01232023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.375.24ng/ul1473.36FALSEFALSEFALSE
lid broke off during elution, not attached, but didn't transfer to new tube
125
2022062136smallcontrol0PSC 012400FALSEFALSEFALSE
126
2022062140smallcontrol0PSC 012500FALSEFALSEFALSE
127
2022062158smallcontrol0PSC 012600FALSEFALSEFALSE
128
2022062159smallcontrol0healthyPSC 01272023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.379.3ng/ul14130.2FALSETRUEFALSE
accidentally pipetted to mix DNase treatment instead of gentle inversion
129
2022062152smallcontrol0PSC 012800FALSEFALSEFALSE
130
2022062156smallcontrol0PSC 012900FALSEFALSEFALSE
131
2022062134smallcontrol0PSC 013000FALSEFALSEFALSE
132
2022062135smallcontrol0healthyPSC 01312023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.37TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
133
2022062155smallcontrol0healthyPSC 01322023-02-27
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.3710.5ng/ul14147FALSETRUETRUE
134
2022062139smallexposed0PSC 013300FALSEFALSEFALSE
135
2022062157smallexposed0PSC 013400FALSEFALSEFALSE
136
2022062143smallexposed0PSC 013500FALSEFALSEFALSE
137
2022062161smallexposed0PSC 013600FALSEFALSEFALSE
138
2022062141smallexposed0PSC 013700FALSEFALSEFALSE
139
2022062153smallexposed0PSC 013800FALSEFALSEFALSE
140
2022062154smallexposed0PSC 013900FALSEFALSEFALSE
141
2022062137smallexposed0PSC 014000FALSEFALSEFALSE
142
2022062144smallexposed0healthyPSC 01412023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.3718.6ng/ul14260.4TRUETRUETRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion
143
2022062147smallexposed0PSC 014200FALSEFALSEFALSE
144
2022062142smallexposed0PSC 014300FALSEFALSEFALSE
145
2022062321largeexposed2PSC 01442023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.37TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion
star has been looking funny since 6/19/2022, used in experiment as 0.22 inoc.; didn't sample control pair because this star likely got sick well before our experiment start; for PSC: couldn't get much so didn't spin down - just added RNAlater
146
2022062959smallcontrol8PSC 01452023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.374.6ng/ul1464.4FALSEFALSEFALSE
accidentally pipetted to mix DNase treatment instead of gentle inversion
147
2022062914largecontrol8PSC 01462023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-02182.151717.376.62ng/ul1492.68FALSEFALSEFALSE
accidentally pipetted to mix DNase treatment instead of gentle inversion
148
2022062929largecontrol8PSC 01472023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.124ng/ul1456FALSEFALSEFALSE
accidentally pipetted to mix DNase treatment instead of gentle inversion; more than 15ul eluted... some buffer got in probably
149
2022062944smallexposed8PSC 01482023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion
150
2022062928largeexposed8
FIRST arm dropped, 1 arm twisted
PSC 01492023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.1212.6ng/ul14176.4FALSETRUETRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion; more than 15ul eluted... some buffer got in probably
151
202206296largeexposed8
FIRST arm dropped, 4 arms twisted
PSC 01502023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.1211.3ng/ul14158.2FALSETRUETRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion
152
2022063040smallcontrol9healthyPSC 01512023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.127.36ng/ul14103.04FALSETRUEFALSE
accidentally pipetted to mix DNase treatment instead of gentle inversion
153
2022063059smallcontrol9PSC 015200FALSEFALSEFALSE
154
2022063014largecontrol9PSC 01532023-03-29
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-291NANANAng/ulNAng/ul13#VALUE!#VALUE!#VALUE!FALSE
removed 1ul for qubit, but messed up math, so will have to re-do qubit
155
2022063039smallexposed9PSC 01542023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion
lost 2 more arms since AM
156
2022063044smallexposed9PSC 01552023-03-29
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-291NANANAng/ul6.96ng/ul1390.48FALSEFALSEFALSE
removed 1ul for qubit, but messed up math, so will have to re-do qubit
157
2022063028largeexposed9
SECOND arm dropped
PSC 01562023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.1215.6ng/ul14218.4FALSETRUETRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion
histo is through lesioned section on arm; dropped 1 arm
158
2022063051smallcontrol9PSC 01572023-03-01
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
accidentally pipetted to mix DNase treatment instead of gentle inversion; more than 15ul eluted, some buffer in prob
159
2022063036smallcontrol9PSC 01582023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
160
2022063050smallexposed9lost 6 armsPSC 01592023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
lost 6 arms
161
2022063047smallexposed9lost 4 armsPSC 01602023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
lost 4 arms
162
2022070134smallcontrol10healthyPSC 01612023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.1211.5ng/ul14161FALSETRUETRUE
used DNAse I from two different kits for DNAse treatment
163
2022070152smallcontrol10PSC 01622023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
164
2022070140smallcontrol10healthyPSC 01632023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.129.1ng/ul14127.4FALSETRUEFALSE
used DNAse I from two different kits for DNAse treatment
sublethal paired sampling - don't take arm
165
2022070136smallcontrol10healthyPSC 01642023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.129.8ng/ul14137.2FALSETRUEFALSE
used DNAse I from two different kits for DNAse treatment
sublethal paired sampling - don't take arm
166
2022070151smallcontrol10PSC 01652023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
sublethal paired sampling - don't take arm
167
2022070159smallcontrol10PSC 01662023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
sublethal paired sampling - don't take arm
168
2022070154smallexposed10lost 4 armsPSC 01672023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
lost 4 arms
169
2022070137smallexposed10lost 6 armsPSC 01682023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.124.2ng/ul1458.8FALSEFALSEFALSE
used DNAse I from two different kits for DNAse treatment
lost 6 arms
170
2022070139smallexposed10lost 5 armsPSC 01692023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
lost 5 arms
171
2022070147smallexposed10lost 5 armsPSC 01702023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
lost 5 arms
172
2022070150smallexposed10lost 6 armsPSC 01712023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
lost 6 arms
173
2022070144smallexposed10
lost 5 arms (didin't keep third arm (PSA) because this is third sampling)
PSC 01722023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.12TOO LOWng/ul14#VALUE!#VALUE!#VALUE!TRUE
used DNAse I from two different kits for DNAse treatment
lost 5 arms (didin't keep third arm (PSA) because this is third sampling)
174
2022070144smallexposed10PSC 01722023-03-06
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
151140FALSEFALSEFALSE
not taking third arm
175
2022070131smallcontrol10healthyPSC 01732023-03-29
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-291NANANAng/ul14.5ng/ul13188.5FALSETRUETRUE
removed 1ul for qubit, but messed up math, so will have to re-do qubit
176
2022070130largeexposed10
FIRST arm dropped, 6 arms twisted
PSC 01742023-03-02
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06181.011755.1210.6ng/ul14148.4FALSETRUETRUE
used DNAse I from two different kits for DNAse treatment
no lesion swab was collected in RNAlater
177
2022070252smallcontrol11PSC 01752023-03-06
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06177.33159.41TOO LOWng/ul4.4ng/ul1357.2FALSEFALSEFALSE
standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples
sublethal paired sampling
178
2022070237smallexposed11PSC 01762023-03-06
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06177.33159.41TOO LOWng/ulTOO LOWng/ul13#VALUE!#VALUE!#VALUE!FALSE
standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples
sublethal paired sampling
179
2022070234smallcontrol11healthyPSC 01772023-03-06
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06177.33159.4112.2ng/ul27.2ng/ul13353.6TRUETRUETRUE
standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples
sublethal paired sampling
180
2022070254smallexposed11PSC 01782023-03-06
Zymo-quickDNA/RNA-microprep
RT DNAse-RNAse free H20
152023-03-06177.33159.41TOO LOWng/ulTOO LOWng/ul13#VALUE!#VALUE!#VALUE!FALSE
standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples
sublethal paired sampling; forgot to do histology
181
2022070236smallcontrol11PSC 017900FALSEFALSE
sublethal paired sampling
182
2022070252smallcontrol11PSC 018000FALSEFALSEno arm no histo
183
2022070259smallcontrol11PSC 018100FALSEFALSEsublethal
184
2022070234smallcontrol11PSC 018200FALSEFALSEno arm no histo