A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | AA | AB | AC | AD | AE | AF | AG | AL | AM | AN | AO | AP | AQ | AR | AS | AT | AU | AV | AW | AX | AY | AZ | BA | ||
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1 | sample_date | star_ID | star_size | treatment_grp | experiment_day | disease_sign | coelom_ID | extraction_date | kit | elution_liquid | elution_vol_ul | qubit_run_date | ul_run_on_qubit | qubit_std_1 | qubit_std_2 | RNA_readout | units | RNA_2 | units | final_vol_ul | total-RNA_ng | morethan_250ng_RNA_yes_no | morethan_100ng_total_RNA_yes_no | conc_original_qubt_10ngul_t_o_f | send_for_seq_yes-or-no | notes | RNA_-080C_yes_no | notes_on_star_at_sampling_time | |||||||||||||
86 | 20220621 | 6 | large | exposed | 0 | healthy | PSC 0085 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 9.92 | ng/ul | 14 | 138.88 | FALSE | TRUE | FALSE | |||||||||||||||||||
87 | 20220621 | 29 | large | control | 0 | healthy | PSC 0086 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 19.7 | ng/ul | 14 | 275.8 | TRUE | TRUE | TRUE | |||||||||||||||||||
88 | 20220621 | 31 | large | control | 0 | healthy | PSC 0087 | 2023-03-09 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-09 | 1 | 82.14 | 1565.32 | 20.4 | ng/ul | 14 | 285.6 | TRUE | TRUE | TRUE | treated cells like blood and did modified protocol | ||||||||||||||||||
89 | 20220621 | 11 | large | exposed | 0 | healthy | PSC 0088 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 5.64 | ng/ul | 14 | 78.96 | FALSE | FALSE | FALSE | |||||||||||||||||||
90 | 20220621 | 18 | large | exposed | 0 | healthy | PSC 0089 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 5.22 | ng/ul | 14 | 73.08 | FALSE | FALSE | FALSE | |||||||||||||||||||
91 | 20220621 | 27 | large | control | 0 | healthy | PSC 0090 | 2023-03-09 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-09 | 1 | 82.14 | 1565.32 | 9.62 | ng/ul | 14 | 134.68 | FALSE | TRUE | FALSE | treated cells like blood and did modified protocol | ||||||||||||||||||
92 | 20220621 | 19 | large | exposed | 0 | healthy | PSC 0091 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 9.66 | ng/ul | 14 | 135.24 | FALSE | TRUE | FALSE | |||||||||||||||||||
93 | 20220621 | 26 | large | control | 0 | PSC 0092 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
94 | 20220621 | 13 | large | exposed | 0 | healthy | PSC 0093 | 2023-03-09 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-09 | 1 | 82.14 | 1565.32 | 84.8 | ng/ul | 14 | 1187.2 | TRUE | TRUE | TRUE | treated cells like blood and did modified protocol | ||||||||||||||||||
95 | 20220621 | 33 | large | control | 0 | healthy | PSC 0094 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 9.38 | ng/ul | 14 | 131.32 | FALSE | TRUE | FALSE | |||||||||||||||||||
96 | 20220621 | 10 | large | exposed | 0 | healthy | PSC 0095 | 2023-03-09 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-09 | 1 | 82.14 | 1565.32 | 70.2 | ng/ul | 14 | 982.8 | TRUE | TRUE | TRUE | treated cells like blood and did modified protocol | ||||||||||||||||||
97 | 20220621 | 4 | large | control | 0 | healthy | PSC 0096 | 2023-03-29 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-29 | 1 | NA | NA | NA | ng/ul | 78.6 | ng/ul | 13 | 1021.8 | TRUE | TRUE | TRUE | removed 1ul for qubit, but messed up math, so will have to re-do qubit | arms twisted - 7 | |||||||||||||||
98 | 20220621 | 12 | large | exposed | 0 | PSC 0097 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
99 | 20220621 | 32 | large | control | 0 | PSC 0098 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
100 | 20220621 | 28 | large | exposed | 0 | healthy | PSC 0099 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 26.2 | ng/ul | 14 | 366.8 | TRUE | TRUE | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion | ||||||||||||||||||
101 | 20220621 | 14 | large | control | 0 | PSC 0100 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 6.82 | ng/ul | 14 | 95.48 | FALSE | FALSE | FALSE | accidentally pipetted to mix DNase treatment instead of gentle inversion | |||||||||||||||||||
102 | 20220621 | 30 | large | exposed | 0 | PSC 0101 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
103 | 20220621 | 15 | large | control | 0 | PSC 0102 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
104 | 20220621 | 22 | large | exposed | 0 | PSC 0103 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
105 | 20220621 | 3 | large | exposed | 0 | PSC 0104 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
106 | 20220621 | 23 | large | control | 0 | healthy | PSC 0105 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 43.5 | ng/ul | 14 | 609 | TRUE | TRUE | TRUE | after removal of RNAlater supernatant, the pellet was smaller and not white like the others | uneaten mussels | |||||||||||||||||
107 | 20220621 | 5 | large | exposed | 0 | PSC 0106 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
108 | 20220621 | 7 | large | control | 0 | PSC 0107 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
109 | 20220621 | 20 | large | control | 0 | PSC 0108 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
110 | 20220621 | 17 | large | control | 0 | PSC 0109 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
111 | 20220621 | 25 | large | exposed | 0 | PSC 0110 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
112 | 20220621 | 8 | large | control | 0 | healthy | PSC 0111 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 7.38 | ng/ul | 14 | 103.32 | FALSE | TRUE | FALSE | step 1 of purification --> had to spin 4x to get all sample through yellow filter, was clogging it | ||||||||||||||||||
113 | 20220621 | 9 | large | control | 0 | PSC 0112 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
114 | 20220621 | 21 | large | exposed | 0 | PSC 0113 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 6.86 | ng/ul | 14 | 96.04 | FALSE | FALSE | FALSE | accidentally pipetted to mix DNase treatment instead of gentle inversion | stretched | ||||||||||||||||||
115 | 20220621 | 46 | small | exposed | 0 | healthy | PSC 0114 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 5.42 | ng/ul | 14 | 75.88 | FALSE | FALSE | FALSE | |||||||||||||||||||
116 | 20220621 | 50 | small | exposed | 0 | healthy | PSC 0115 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | |||||||||||||||||||
117 | 20220621 | 38 | small | exposed | 0 | healthy | PSC 0116 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | |||||||||||||||||||
118 | 20220621 | 48 | small | exposed | 0 | healthy | PSC 0117 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | |||||||||||||||||||
119 | 20220621 | 60 | small | control | 0 | healthy | PSC 0118 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 10.9 | ng/ul | 14 | 152.6 | FALSE | TRUE | TRUE | |||||||||||||||||||
120 | 20220621 | 63 | small | control | 0 | healthy | PSC 0119 | 2023-03-29 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-29 | 1 | NA | NA | NA | ng/ul | 81.6 | ng/ul | 13 | 1060.8 | TRUE | TRUE | TRUE | removed 1ul for qubit, but messed up math, so will have to re-do qubit | ||||||||||||||||
121 | 20220621 | 62 | small | control | 0 | PSC 0120 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
122 | 20220621 | 45 | small | control | 0 | PSC 0121 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
123 | 20220621 | 49 | small | control | 0 | PSC 0122 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
124 | 20220621 | 51 | small | control | 0 | healthy | PSC 0123 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 5.24 | ng/ul | 14 | 73.36 | FALSE | FALSE | FALSE | lid broke off during elution, not attached, but didn't transfer to new tube | ||||||||||||||||||
125 | 20220621 | 36 | small | control | 0 | PSC 0124 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
126 | 20220621 | 40 | small | control | 0 | PSC 0125 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
127 | 20220621 | 58 | small | control | 0 | PSC 0126 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
128 | 20220621 | 59 | small | control | 0 | healthy | PSC 0127 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 9.3 | ng/ul | 14 | 130.2 | FALSE | TRUE | FALSE | accidentally pipetted to mix DNase treatment instead of gentle inversion | ||||||||||||||||||
129 | 20220621 | 52 | small | control | 0 | PSC 0128 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
130 | 20220621 | 56 | small | control | 0 | PSC 0129 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
131 | 20220621 | 34 | small | control | 0 | PSC 0130 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
132 | 20220621 | 35 | small | control | 0 | healthy | PSC 0131 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | |||||||||||||||||||
133 | 20220621 | 55 | small | control | 0 | healthy | PSC 0132 | 2023-02-27 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 10.5 | ng/ul | 14 | 147 | FALSE | TRUE | TRUE | |||||||||||||||||||
134 | 20220621 | 39 | small | exposed | 0 | PSC 0133 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
135 | 20220621 | 57 | small | exposed | 0 | PSC 0134 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
136 | 20220621 | 43 | small | exposed | 0 | PSC 0135 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
137 | 20220621 | 61 | small | exposed | 0 | PSC 0136 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
138 | 20220621 | 41 | small | exposed | 0 | PSC 0137 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
139 | 20220621 | 53 | small | exposed | 0 | PSC 0138 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
140 | 20220621 | 54 | small | exposed | 0 | PSC 0139 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
141 | 20220621 | 37 | small | exposed | 0 | PSC 0140 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
142 | 20220621 | 44 | small | exposed | 0 | healthy | PSC 0141 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 18.6 | ng/ul | 14 | 260.4 | TRUE | TRUE | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion | ||||||||||||||||||
143 | 20220621 | 47 | small | exposed | 0 | PSC 0142 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
144 | 20220621 | 42 | small | exposed | 0 | PSC 0143 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
145 | 20220623 | 21 | large | exposed | 2 | PSC 0144 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion | star has been looking funny since 6/19/2022, used in experiment as 0.22 inoc.; didn't sample control pair because this star likely got sick well before our experiment start; for PSC: couldn't get much so didn't spin down - just added RNAlater | ||||||||||||||||||
146 | 20220629 | 59 | small | control | 8 | PSC 0145 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 4.6 | ng/ul | 14 | 64.4 | FALSE | FALSE | FALSE | accidentally pipetted to mix DNase treatment instead of gentle inversion | |||||||||||||||||||
147 | 20220629 | 14 | large | control | 8 | PSC 0146 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-02 | 1 | 82.15 | 1717.37 | 6.62 | ng/ul | 14 | 92.68 | FALSE | FALSE | FALSE | accidentally pipetted to mix DNase treatment instead of gentle inversion | |||||||||||||||||||
148 | 20220629 | 29 | large | control | 8 | PSC 0147 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 4 | ng/ul | 14 | 56 | FALSE | FALSE | FALSE | accidentally pipetted to mix DNase treatment instead of gentle inversion; more than 15ul eluted... some buffer got in probably | |||||||||||||||||||
149 | 20220629 | 44 | small | exposed | 8 | PSC 0148 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion | |||||||||||||||||||
150 | 20220629 | 28 | large | exposed | 8 | FIRST arm dropped, 1 arm twisted | PSC 0149 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 12.6 | ng/ul | 14 | 176.4 | FALSE | TRUE | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion; more than 15ul eluted... some buffer got in probably | ||||||||||||||||||
151 | 20220629 | 6 | large | exposed | 8 | FIRST arm dropped, 4 arms twisted | PSC 0150 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 11.3 | ng/ul | 14 | 158.2 | FALSE | TRUE | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion | ||||||||||||||||||
152 | 20220630 | 40 | small | control | 9 | healthy | PSC 0151 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 7.36 | ng/ul | 14 | 103.04 | FALSE | TRUE | FALSE | accidentally pipetted to mix DNase treatment instead of gentle inversion | ||||||||||||||||||
153 | 20220630 | 59 | small | control | 9 | PSC 0152 | 0 | 0 | FALSE | FALSE | FALSE | ||||||||||||||||||||||||||||||
154 | 20220630 | 14 | large | control | 9 | PSC 0153 | 2023-03-29 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-29 | 1 | NA | NA | NA | ng/ul | NA | ng/ul | 13 | #VALUE! | #VALUE! | #VALUE! | FALSE | removed 1ul for qubit, but messed up math, so will have to re-do qubit | |||||||||||||||||
155 | 20220630 | 39 | small | exposed | 9 | PSC 0154 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion | lost 2 more arms since AM | ||||||||||||||||||
156 | 20220630 | 44 | small | exposed | 9 | PSC 0155 | 2023-03-29 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-29 | 1 | NA | NA | NA | ng/ul | 6.96 | ng/ul | 13 | 90.48 | FALSE | FALSE | FALSE | removed 1ul for qubit, but messed up math, so will have to re-do qubit | |||||||||||||||||
157 | 20220630 | 28 | large | exposed | 9 | SECOND arm dropped | PSC 0156 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 15.6 | ng/ul | 14 | 218.4 | FALSE | TRUE | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion | histo is through lesioned section on arm; dropped 1 arm | |||||||||||||||||
158 | 20220630 | 51 | small | control | 9 | PSC 0157 | 2023-03-01 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | accidentally pipetted to mix DNase treatment instead of gentle inversion; more than 15ul eluted, some buffer in prob | |||||||||||||||||||
159 | 20220630 | 36 | small | control | 9 | PSC 0158 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | |||||||||||||||||||
160 | 20220630 | 50 | small | exposed | 9 | lost 6 arms | PSC 0159 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | lost 6 arms | |||||||||||||||||
161 | 20220630 | 47 | small | exposed | 9 | lost 4 arms | PSC 0160 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | lost 4 arms | |||||||||||||||||
162 | 20220701 | 34 | small | control | 10 | healthy | PSC 0161 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 11.5 | ng/ul | 14 | 161 | FALSE | TRUE | TRUE | used DNAse I from two different kits for DNAse treatment | ||||||||||||||||||
163 | 20220701 | 52 | small | control | 10 | PSC 0162 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | |||||||||||||||||||
164 | 20220701 | 40 | small | control | 10 | healthy | PSC 0163 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 9.1 | ng/ul | 14 | 127.4 | FALSE | TRUE | FALSE | used DNAse I from two different kits for DNAse treatment | sublethal paired sampling - don't take arm | |||||||||||||||||
165 | 20220701 | 36 | small | control | 10 | healthy | PSC 0164 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 9.8 | ng/ul | 14 | 137.2 | FALSE | TRUE | FALSE | used DNAse I from two different kits for DNAse treatment | sublethal paired sampling - don't take arm | |||||||||||||||||
166 | 20220701 | 51 | small | control | 10 | PSC 0165 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | sublethal paired sampling - don't take arm | ||||||||||||||||||
167 | 20220701 | 59 | small | control | 10 | PSC 0166 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | sublethal paired sampling - don't take arm | ||||||||||||||||||
168 | 20220701 | 54 | small | exposed | 10 | lost 4 arms | PSC 0167 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | lost 4 arms | |||||||||||||||||
169 | 20220701 | 37 | small | exposed | 10 | lost 6 arms | PSC 0168 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 4.2 | ng/ul | 14 | 58.8 | FALSE | FALSE | FALSE | used DNAse I from two different kits for DNAse treatment | lost 6 arms | |||||||||||||||||
170 | 20220701 | 39 | small | exposed | 10 | lost 5 arms | PSC 0169 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | lost 5 arms | |||||||||||||||||
171 | 20220701 | 47 | small | exposed | 10 | lost 5 arms | PSC 0170 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | lost 5 arms | |||||||||||||||||
172 | 20220701 | 50 | small | exposed | 10 | lost 6 arms | PSC 0171 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | lost 6 arms | |||||||||||||||||
173 | 20220701 | 44 | small | exposed | 10 | lost 5 arms (didin't keep third arm (PSA) because this is third sampling) | PSC 0172 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | TOO LOW | ng/ul | 14 | #VALUE! | #VALUE! | #VALUE! | TRUE | used DNAse I from two different kits for DNAse treatment | lost 5 arms (didin't keep third arm (PSA) because this is third sampling) | |||||||||||||||||
174 | 20220701 | 44 | small | exposed | 10 | PSC 0172 | 2023-03-06 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 1 | 14 | 0 | FALSE | FALSE | FALSE | not taking third arm | ||||||||||||||||||||||||
175 | 20220701 | 31 | small | control | 10 | healthy | PSC 0173 | 2023-03-29 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-29 | 1 | NA | NA | NA | ng/ul | 14.5 | ng/ul | 13 | 188.5 | FALSE | TRUE | TRUE | removed 1ul for qubit, but messed up math, so will have to re-do qubit | ||||||||||||||||
176 | 20220701 | 30 | large | exposed | 10 | FIRST arm dropped, 6 arms twisted | PSC 0174 | 2023-03-02 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 81.01 | 1755.12 | 10.6 | ng/ul | 14 | 148.4 | FALSE | TRUE | TRUE | used DNAse I from two different kits for DNAse treatment | no lesion swab was collected in RNAlater | |||||||||||||||||
177 | 20220702 | 52 | small | control | 11 | PSC 0175 | 2023-03-06 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 77.33 | 159.41 | TOO LOW | ng/ul | 4.4 | ng/ul | 13 | 57.2 | FALSE | FALSE | FALSE | standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples | sublethal paired sampling | ||||||||||||||||
178 | 20220702 | 37 | small | exposed | 11 | PSC 0176 | 2023-03-06 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 77.33 | 159.41 | TOO LOW | ng/ul | TOO LOW | ng/ul | 13 | #VALUE! | #VALUE! | #VALUE! | FALSE | standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples | sublethal paired sampling | ||||||||||||||||
179 | 20220702 | 34 | small | control | 11 | healthy | PSC 0177 | 2023-03-06 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 77.33 | 159.41 | 12.2 | ng/ul | 27.2 | ng/ul | 13 | 353.6 | TRUE | TRUE | TRUE | standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples | sublethal paired sampling | |||||||||||||||
180 | 20220702 | 54 | small | exposed | 11 | PSC 0178 | 2023-03-06 | Zymo-quickDNA/RNA-microprep | RT DNAse-RNAse free H20 | 15 | 2023-03-06 | 1 | 77.33 | 159.41 | TOO LOW | ng/ul | TOO LOW | ng/ul | 13 | #VALUE! | #VALUE! | #VALUE! | FALSE | standard 2 weird, so I ran the samples without the standards for the first Qubit run; UPDATE 03082023: reran a round of qubut of just standards and new master mix and standards looked great, so i was making errors with the master mix. need to re-run all these samples | sublethal paired sampling; forgot to do histology | ||||||||||||||||
181 | 20220702 | 36 | small | control | 11 | PSC 0179 | 0 | 0 | FALSE | FALSE | sublethal paired sampling | ||||||||||||||||||||||||||||||
182 | 20220702 | 52 | small | control | 11 | PSC 0180 | 0 | 0 | FALSE | FALSE | no arm no histo | ||||||||||||||||||||||||||||||
183 | 20220702 | 59 | small | control | 11 | PSC 0181 | 0 | 0 | FALSE | FALSE | sublethal | ||||||||||||||||||||||||||||||
184 | 20220702 | 34 | small | control | 11 | PSC 0182 | 0 | 0 | FALSE | FALSE | no arm no histo |