Primers_MBD

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P
1	Category	Gene	Forward primer	Reverse primer	Comp	Date	MeltF	MeltR	Gccont F	GCcontR	Product size	Exon spanning	OrderDate	Reference	Responsbile	Note
2	Housekeep	GAPDH	AAGTATGACAACAGCCTCAA	TCCTTCCACGATACCAAAGT			54.58	56.17	40	45	163	YES		Vinarsky2025	Cris
3	CM-Maturation	MLC-2a	AGGAGTTCAAAGAAGCCTTCAGCT	AGCATGGCGTCCAGCTCCTC			62.28	64.73	45.83	65	130	YES		Vinarsky2025	Cris
4	CM-Maturation	MLC-2v	TCTGAGAGACACCTTTGCTGCC	GGGTCCGCTCCCTTAAGTTTCT			62.51	62.01	54.55	54.55	141	YES		Vinarsky2025	Cris
5	CM-Maturation	MYH6	GGAAGACAAGGTCAACAGCCTG	TCCAGTTTCCGCTTTGCTCGCT			61.39	65.26	54.55	54.55	129	YES		Vinarsky2025	Cris	too close to MYH7
6	CM-Maturation	MYH7	TCGTGCCTGATGACAAACAGG	ATACTCGGTCTCGGCAGTGACT			60.88	62.9	52.38	54.55	83	NO		Vinarsky2025	Cris
7	CM-Maturation	TNNTI 1	GTGGGTGACTGGAGGAAGAA	GTGAGCTGGGTTGGAGAAGA										Vinarsky2025	Cris
8	CM-Maturation	TNNTI 3	CACCTCAAGCAGGTGAAGAAG	CAGGAAGGCTCAGCTCTCAA			58.85	59.39	52.38	55	129	YES		Vinarsky2025	Cris
9	CM-Progenitor	MESP1	CTCTGTTGGAGACCTGGATG	CCTGCTTGCCTCAAAGTG			57.31	56.31	55	55.56	278	NO
10	CM-Progenitor	EOMES	CGGCCTCTGTGGCTCAAA	AAGGAAACATGCGCCTGC			59.97	59.04	61.11	55.56	76	YES
11	Hypertrophy	TBX6_1	CATCCACGAGAATTGTACCCG	AGCAATCCAGTTTAGGGGTGT			59.29	58.8	52.38	47.62	139	YES	6/3/2020
12	Hypertrophy	TBX6_2	CATCCACGAGAATTGTACCCG	AGCAATCCAGTTTAGGGGTGT			59.29	58.8	52.38	47.62	139	YES	17/10/2019
13	Epigenetics	DOT1L	TCGTCCACACTTGAAAAGCAGAT	CACCGAGCCAGCGTAGGA			60.74	61.45	61.45	66.67	99	YES
14	Hand written primer sequence
15	Proliferation	Cyclin D (sigma)	CGGAGGAGAACAAACAGATC	AGGGCGGATTGGAAATGAA			61.3-- 55.88	66.4--57.00	50	47.37	90	YES
16	CM-Progenitor	Nkx2.5 (sigma)	GCCGAAAAGAAAGGGTGAA	AAAGTCAGGCTGGCTCAAGG			67.1	65.6								Hand written primer sequence
17	CM-Progenitor	ISL-1 (Sigma)	TGTGCGGAGTGTAATCAGTA	TTTGATCCCGTACAACCTGA			59.3--56.30	62.9--56.47	45	45	102	YES				Hand written primer sequence
18	CM-Progenitor	TBX5 (Invitrogen)	ACATGGAGCTGCACAGAATG	TGCTGAAAGGACTGTGGTTG			n/a	n/a								Hand written primer sequence
19	Maturation	MYH6 (Sigma)	CTTTGACATTCGCACTGAGT	TCTTCCCATTCTCGGTTTCA			60.5	64.5								Hand written primer sequence
20	Splicing	RBM20 (SIGMA)	CCTACCCCAGATCATCCAAAATGC	AACAAACACTTTGCAGTCAGTTATACA			69.9	63.5								Hand written primer sequence
21	Splicing	TTN EX (107-108)	CAGCAGAACTCAGAATCGA	ATCAAAGGACACTTCACTC			59.2	58.2								Hand written primer sequence
22	Splicing	TTN EX (49-50)	GTAAAAAGAGCTGCCCCAGTGA	GCTAGGTGGCCCAGTGCTACT			66.3	66.6								Hand written primer sequence
23	Splicing	TTN EX (50-219)	CCAATGAGTATGGCAGTGTCA	TACGTTCCGGAAGTAATTTGC			63.6	62.6								Hand written primer sequence
24	Splicing	TTN EX (54-55)	GGAATTAATAGTTAAAGAACCTGCCAAA	CTCCTGCTGTCACCTGGATCA			64.7	67.8								Hand written primer sequence
25	Splicing	TTN EX (50-60)	CCAATGAGTATGGCAGTGTCA	CCGGAGGGAGCTGGTACTC			63.6	66.1								Hand written primer sequence
26	Splicing	TTN EX (50-51)	GCCACACTAACTGTGACAGAGG	GGCTGCCTTACCCACAAAAG			63.7	65.6								Hand written primer sequence
27	Splicing	TTN EX (50-77)	GCTTCCAATGAGTATGGCAGTGT	CGTAACAAAATAAGGCGGTTCTGTC			66.2	67.1								Hand written primer sequence
28	Maturation	h_MYH6_1	GAGCTCACCTACCAGACAGAG	GGCAGTGTCACTCCTCATCG											VV
29	Maturation	h_MYH6_2	TTCAGGATTCTCCGTGAAGGG	GTCGAACTTGGGTGGGTTCT											VV
30	Maturation	h_myh7_1	GACAGGAAAAACCTGCTGCG	TCATTCAAGCCCTTCGTGCC											VV
31	Maturation	h_myh7_2	TTGGCACGAAGGGCTTGAA	TTCCTCCCAAGGAGCTGTTAC											VV
32	Calcium	h_SERCA2_1	CGAACCCTTGCCACTCATCT	TAGACCCAGATCACCAGGGG											VV
33	Calcium	h_SERCA2_2	CCCTCAACAGCTTGTCCGAA	GCCGAGAACGAGCAGGATTT											VV
34	Hypertrophy	h_NPPB_1	CTTTCCTGGGAGGTCGTTCC	GTTGCGCTGCTCCTGTAAC											VV
35	Hypertrophy	h_NPPB_2	CCCACAGGTGTCTGGAAGTC	TTAATGCCGCCTCAGCACTTT											VV
36	Hypertrophy	h_NPPA_1	TCCTCTGATCGATCTGCCCT	CTTCAGTACCGGAAGCTGTT											VV
37	Hypertrophy	h_NPPA_2	CACCGTGAGCTTCCTCCTTT	CCAAATGGTCCAGCAAATTCTTG											VV
38	Sarcomere	h_ACTA1_1	CCCGCCCAGAAACTAGACAC	ATGATGCCGTGCTCGATAGG											VV
39	Sarcomere	h_ACTA1_2	AAGATCAAGATCATCGCCCCG	GTTTACGATGGCAGCAACGG											VV
40	Maturation	h_MLC2a_1	AAGCCATCCTGAGTGCCTTC	AACATCTGCTCCACCTCAGC											VV
41	Maturation	h_MLC2a_2	CATCTGCAAGGCAGACCTGA	AACATCTGCTCCACCTCAGC											VV
42	Maturation	h_MLC2v_1	AGGCTCCGGGTCCAATTAAC	AGGGTCCGCTCCCTTAAGTT											VV
43	Maturation	h_MLC2v_2	AAGGCTGATTACGTTCGGGA	AGGTGGATAAATGGGGCAGC											VV
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