The following list of gene / proteins and technologies can be used as a reference for what current cell-based meat companies have discussed in patents / patent applications. It should not be used to understand where companies have protectability. For that, consult an IP lawyer.

"Experimentally validated" means that the company describes an experiment involving the gene / protein in question.

Cell Type, How Used, Product Application, and Species are noted if:
1) The technology described in the patent or patent application is targeted at a specific use case,
2) The experiments described specify any of these data
Keep in mind that often the IP attempts to cover use cases much broader than what is described in the experiments.

This community resource is being kept up to date (last update 09/02/19). For suggestions, additions, or corrections, contact robert.e.yaman@gmail.com

Product Application (if specified)

Spontaneously Immortalized Fibroblast

Dox-inducible. In Google patents, notated as "myoDl". Sequence here: https://patentscope.wipo.int/search/docs2/pct/WO2018011805/file/WgrRpwlpTJzsJvmC38gBRNjiGwEAW4KkwOAUWzxJ38hazEYk4XeiGo5aQPB6YHptlSn6cXE7Ox-bao5QpVoVtZxrdktzAEWPYQKNj-0KnF1KFrinnRA-g4z_puq632maWWgIA28R7UMhJc81pxdg4Q-GfhhLyTPPoNryRPfRf5U?docId=id00000040900805&psAuth=74OixcPs-tXHCdY4kFhTL0wmgIXaa3rgI3librw1Xf8

Spontaneously Immortalized Fibroblast

Sequence here: https://patentscope.wipo.int/search/docs2/pct/WO2018011805/file/WgrRpwlpTJzsJvmC38gBRNjiGwEAW4KkwOAUWzxJ38hazEYk4XeiGo5aQPB6YHptlSn6cXE7Ox-bao5QpVoVtZxrdktzAEWPYQKNj-0KnF1KFrinnRA-g4z_puq632maWWgIA28R7UMhJc81pxdg4Q-GfhhLyTPPoNryRPfRf5U?docId=id00000040900805&psAuth=74OixcPs-tXHCdY4kFhTL0wmgIXaa3rgI3librw1Xf8

Spontaneously Immortalized Fibroblast

Ectopically expressed then activated via small molecule

Example 8 includes Dox-inducible vector. PPARg is a transcription factor that can influence genes related to adipogenesis (e.g., ADIPOQ, LPL, NR1H3, and UCP1); genes related to fatty Acid Metabolism (e.g., ACADL, AC ADM, ACOX1, ACOX3, ACSL1, ACSL3, ACSL4, ACSL5, CPT1A, CPT1B, CPT2, CYP27A1, CYP4A11, CYP7A1, EHHADH, FADS2, GK, and SCD); genes related to lipid transport (e.g., ADIPOQ, ANGPTL4, APOE, DGAT1, LPL, NR1H3, and OLR1); genes related to cell proliferation (e.g., CLU, ELN, HSPD1, and TXNIP); genes related to insulin signaling (e.g., CPT1A, DGAT1, PCK1, and SORBS 1) and other genes such as MMP9 and PCK1.

Fused with ESR1 ligand binding domain for E2 induction

Not experimentally validated in the patent application

Not experimentally validated in the patent application

Not experimentally validated in the patent application

Not experimentally validated in the patent application

Not experimentally validated in the patent application

CTNNB1 is usually a phosphorylation target of GSK3-beta. When GSK3-beta is inhibited, CTNNB1 remains active. An alternative to inhibiting GSK3-beta is modying CTNNB1 directly to be constitutively active.

Transfected with construct allowing for Tet-on induction

Transfected with construct allowing for Tet-on induction

Direct expression with genetic construct

CRISPR Indel mutations knock out CGK2NA gene, which normally inhibits p16

CRISPR Indel mutations knock out CGK2NB gene, which normally inhibits p15

Direct expression with genetic construct

Fibroblasts (primary or cell lines)

Fibroblasts (primary or cell lines)

Primary fibroblasts and myoblasts

Primary fibroblasts and myoblasts

Primary fibroblasts and myoblasts

Inhibition for Immortalization

Inhibition for Immortalization

Hepatocyte Nuclear Factor 1 Alpha (HNF1 A)

See Table 1 for an organized list of genes and their purpose

Hepatocyte Nuclear Factor 4 Alpha (HNF4A)

Myogenic Differentiation 1 (MyoD)

Myogenic Factor 6 (MRF4)

Myogenic Factor 5 (MYF5)

Fatty Acid Binding Protein 4 (FABP4)

Insulin- Responsive Glucose Transporter Type 4 (GLUT4)

C1Q And Collagen Domain Containing (ADIPOQ)

l-Acylglycerol-3 -Phosphate O-Acyltransferase 2 (AGPAT2)