Bhalla Lab - Primary Antibody Concentrations
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ProteinConcentrationNotes/Special InstructionsSourceLocalizationProduct #
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RbBUB-11:10,000Desai Lab
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RbBUB-31:10,000Desai Lab
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RbCOH-3/COH-41:2500very niceSeverson Lab
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GPDSB-11:500
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RbDSB-21:2500
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MsFLAG tag1:100001:2500 for Western blotsSigma AldrichF1804
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MsGAPDH1:1000Loading control Western blotsCalbiochem (6C5)CB1001
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GtGFP1:10000
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MsGFP1:100Strome Lab
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RbGFP1:1000Use for Western BlotsClontech (LIving Colors)632592
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MsHA tag1:1000Use for Western BlotsSC Biotech (12CA5 clone)SC575-92
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RbH3-S10-Pi1:500active mitotic nuclei (pre-meiotic zone)
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GpHIM-81:250-80°C aliquots labeled as T07G12.12
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RtHIM-81:250-80°C aliquots labeled as T07G12.12
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ChHTP-31:250
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GPHTP-31:500better than chicken when at 1:250
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MsmAb4141:5000
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RbMAD-11:10,000Desai Lab
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RbMAD-21:10,000Desai Lab
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RbPCH-21:500Bhalla Lab
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GPPLK-21:750
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RbRad-511:1000
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RbREC-81:250
Novus, lot # G3048-008A0
29470002-.01mg
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RbSGO-11:25,000Desai Lab
pre-meiotic zone, late pachytene-diakinesis
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GPSUN-1 S8-Pi1:700Jantsch Lab
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GPSYP-11:250
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RbSYP-11:500better than GP...
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MsTubulin1:3500Western Blot Loading Control
Sigma Aldrich (DM1A clone)
T9026
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ChZHP-3BAD
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GPZHP-31:250
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GPZTF-151:5000Bhalla Lab
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RbZIM-11:1000
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GpZIM-21:2500
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GpZIM-31:2000
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