A.cervicornis_DNA_Extractions(May_2017).xlsx
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ABCDEFGHIJKLMNOPQRSTUVWXYZAAABAC
1
TubeSample ID
[DNA] ng/ul (NanoDrop)
[DNA] ng/uL (qubit)
Qubit Date
Qubit Initials
DNA in 8uL
Vol for 77.12ng DNA
Volume of 1:10 dilution
H2O to 8uL
Target Conc
Additional DNA
Additional H2O
Total DNAFinal Vol
Methylation Plate Position
Plate
Abs_01(450nm)
Abs_02(450nm)
Mean Abs
Methylated DNA(ng)
Percent Methylated
2
12h Block 1175.59.6420170510SJW77.128.00
NO DILUTION
0.009.649.600.0017.6017.60G0110.4490.4492.963.84
3
22h Block 2126.516.620170510SJW132.804.65
NO DILUTION
3.355.574.0310.2217.60G0210.1000.1950.1470.620.81
4
32h Block 3420.563.820170510SJW510.401.21
NO DILUTION
6.791.458.152.6617.60A0310.2060.1680.1870.931.21
5
42h Block 4443.553.820170510SJW430.401.43
NO DILUTION
6.571.727.883.1517.60C0310.1220.1090.1160.380.49
6
52h Block 5266.026.220170510SJW209.602.94
NO DILUTION
5.063.536.076.4817.60E0310.1030.1380.1200.410.54
7
62h Block 6259.537.420170510SJW299.202.06
NO DILUTION
5.942.477.134.5417.60G0310.1070.1970.1520.660.86
8
72h Block 8187.02120170511SJW168.003.67
NO DILUTION
4.334.415.198.0817.60A0410.3280.2480.2881.712.22
9
82h Block 9156.513.420170510SJW107.205.76
NO DILUTION
2.246.912.6912.6617.60C0410.1590.1100.1350.530.68
10
92h Block 10153.51620170510SJW128.004.82
NO DILUTION
3.185.783.8210.6017.60E0410.1550.1330.1440.600.78
11
1072h Block 1194.519.420170510SJW155.203.98
NO DILUTION
4.024.774.838.7517.60G0410.2070.2390.2231.211.56
12
1172h Block 2239.526.620170510SJW212.802.90
NO DILUTION
5.103.486.126.3817.60A0510.2150.1750.1950.991.29
13
1272h Block 3198.52520170510SJW200.003.08
NO DILUTION
4.923.705.906.7917.60C0510.1050.0860.0950.220.28
14
1372h Block 42765.018720170510SJW1,496.000.414.123.884.954.659.0717.60E0510.0830.1140.0980.240.32
15
1472h Block 5436.44820170510SJW384.001.61
NO DILUTION
6.391.937.673.5317.60G0510.1210.1790.1500.640.83
16
1572h Block 6438.049.820170510SJW398.401.55
NO DILUTION
6.451.867.743.4117.60A0610.1690.1550.1620.740.96
17
1672h Block 8491.544.820170510SJW358.401.72
NO DILUTION
6.282.077.533.7917.60C0610.1420.0860.1140.360.47
18
1772h Block 9120.520.220170510SJW161.603.82
NO DILUTION
4.184.585.028.4017.60E0610.1660.1410.1540.670.87
19
18
72h Block 10
329.54820170510SJW384.001.61
NO DILUTION
6.391.937.673.5317.60G0610.1400.1430.1420.580.75
20
19D7 Block 1264.536.420170510SJW291.202.12
NO DILUTION
5.882.547.064.6617.60A0710.1140.1060.1100.340.43
21
20D7 Block 2320.525.420170510SJW203.203.04
NO DILUTION
4.963.645.966.6817.60C0710.1080.0860.0970.230.30
22
21D7 Block 3373.043.820170510SJW350.401.76
NO DILUTION
6.242.117.493.8717.60E0710.1220.1060.1140.360.47
23
22D7 Block 4196.034.820170510SJW278.402.22
NO DILUTION
5.782.666.944.8817.60G0710.2100.2530.2321.281.65
24
23D7 Block 5237.529.420170510SJW235.202.62
NO DILUTION
5.383.156.455.7717.60A0810.1610.1290.1450.610.79
25
24D7 Block 6267.534.620170510SJW276.802.23
NO DILUTION
5.772.676.934.9017.60C0810.1040.0780.0910.190.24
26
25D7 Block 8144.519.620170510SJW156.803.93
NO DILUTION
4.074.724.888.6617.60E0810.1230.1150.1190.400.52
27
26D7 Block 9173.518.420170510SJW147.204.19
NO DILUTION
3.815.034.579.2217.60G0810.1660.1850.1760.841.09
28
27D7 Block 10148.527.620170510SJW220.802.79
NO DILUTION
5.213.356.256.1517.60A0910.1730.0920.1320.510.66
29
28D14 Block 1948.019720170510SJW1,576.000.393.914.094.704.908.6117.60C0910.1150.1170.1160.380.50
30
29D14 Block 22748.017520170510SJW1,400.000.444.413.595.294.319.7017.60E0910.1090.1150.1120.350.45
31
30D14 Block 31272.019320170510SJW1,544.000.404.004.004.804.808.7917.60G0910.1320.1670.1500.640.83
32
31D14 Block 4642.018520170510SJW1,480.000.424.173.835.004.609.1717.60A1010.1810.1420.1610.730.95
33
32D14 Block 5511.011520170510SJW920.000.676.711.298.051.5514.7517.60C1010.1230.1190.1210.420.54
34
33D14 Block 61434.015320170510SJW1,224.000.505.042.966.053.5511.0917.60E1010.0980.1110.1040.290.37
35
34D14 Block 8737.514720170510SJW1,176.000.525.252.756.303.3011.5417.60G1010.1600.2160.1880.941.22
36
35D14 Block 9909.588.820170510SJW710.400.87
NO DILUTION
7.131.048.561.9117.60A1110.1940.1310.1620.740.96
37
36
D14 Block 10
448.010220170510SJW816.000.767.560.449.070.5316.6317.60C1110.1260.1180.1220.430.56
38
37D20 Block 1282.044.820170510SJW358.401.72
NO DILUTION
6.282.077.533.7917.60E1110.1060.1090.1070.310.41
39
38D20 Block 2152.538.420170510SJW307.202.01
NO DILUTION
5.992.417.194.4217.60G1110.1460.1980.1720.821.06
40
39D20 Block 3291.059.220170510SJW473.601.30
NO DILUTION
6.701.568.042.8717.60A1210.2160.1430.1800.871.13
41
40D20 Block 4298.037.220170510SJW297.602.07
NO DILUTION
5.932.497.114.5617.60C1210.1340.1300.1320.500.65
42
41D20 Block 5201.535.620170510SJW284.802.17
NO DILUTION
5.832.607.004.7717.60E1210.1210.1560.1390.550.72
43
42D20 Block 6224.07320170510SJW584.001.06
NO DILUTION
6.941.278.332.3217.60G1210.1500.1710.1610.730.94
44
43D20 Block 8123.035.820170510SJW286.402.15
NO DILUTION
5.852.597.014.7417.60G0120.2590.3280.2942.042.65
45
44D20 Block 9121.526.620170510SJW212.802.90
NO DILUTION
5.103.486.126.3817.60G0220.2380.1580.1981.181.53
46
45
D20 Block 10
115.520.820170510SJW166.403.71
NO DILUTION
4.294.455.158.1617.60A0320.0970.1230.1100.390.51
47
46D27 Block 1166.528.220170510SJW225.602.73
NO DILUTION
5.273.286.326.0217.60C0320.1400.1470.1440.700.90
48
47D27 Block 2186.532.420170510SJW259.202.38
NO DILUTION
5.622.866.745.2417.60E0320.4810.4030.4423.384.38
49
48D27 Block 3202.045.420170510SJW363.201.70
NO DILUTION
6.302.047.563.7417.60G0320.1830.2210.2021.221.58
50
49D27 Block 4115.035.220170510SJW281.602.19
NO DILUTION
5.812.636.974.8217.60A0420.1690.1410.1550.801.04
51
50D27 Block 5178.047.620170510SJW380.801.62
NO DILUTION
6.381.947.663.5617.60C0420.1450.1190.1320.590.77
52
51D27 Block 6358.059.420170510SJW475.201.30
NO DILUTION
6.701.568.042.8617.60E0420.1470.1230.1350.620.80
53
52D27 Block 883.020.220170510SJW161.603.82
NO DILUTION
4.184.585.028.4017.60G0420.7890.7980.7936.538.47
54
53D27 Block 9114.53920170510SJW312.001.98
NO DILUTION
6.022.377.234.3517.60A0520.1510.2270.1891.101.43
55
54
D27 Block 10
127.017.820170510SJW142.404.33
NO DILUTION
3.675.204.409.5317.60C0520.1410.1750.1580.831.07
56
55D35 Block 1155.515.320170510SJW122.405.04
NO DILUTION
2.966.053.5511.0917.60E0520.1250.1390.1320.590.77
57
56D35 Block 2163.519.920170510SJW159.203.88
NO DILUTION
4.124.654.958.5317.60G0520.0990.1340.1160.450.59
58
57D35 Block 3122.526.820170510SJW214.402.88
NO DILUTION
5.123.456.156.3317.60A0620.1130.1020.1080.370.48
59
58D35 Block 4149.520.820170510SJW166.403.71
NO DILUTION
4.294.455.158.1617.60C0620.1140.1470.1310.580.75
60
59D35 Block 5216.044.820170510SJW358.401.72
NO DILUTION
6.282.077.533.7917.60E0620.1280.1700.1490.750.97
61
60D35 Block 6169.03320170510SJW264.002.34
NO DILUTION
5.662.806.805.1417.60G0620.1330.1300.1320.590.77
62
61D35 Block 865.020.620170511SJW164.803.74
NO DILUTION
4.264.495.118.2417.60A0720.3400.2120.2761.892.45
63
62D35 Block 9636.019.320170510SJW154.404.00
NO DILUTION
4.004.804.808.7917.60C0720.0880.1270.1080.380.49
64
65
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Sheet1