Public ERCC U19 Resources Catalog v1.0
 Share
The version of the browser you are using is no longer supported. Please upgrade to a supported browser.Dismiss

 
View only
 
 
ABCDEFGHIJKLMNOPQRSTUVWXYZAAAB
1
Contact PI Co-PI Availability (or date avail)Reagents, etc.Description, etc.PlasmidsCellsMiceDatasetsLibrariesCommentsPoint of Contact emailPublication, PMID (if available)
2
BreakefieldBreakefieldYlentivirus vectors expressing palmtdTomato and palm GFPlabels all membranes in cells and membranes of EVs; tdTomato is brighter than GFP versionBreakefield@hms.harvard.eduPMC4437317
3
BreakefieldBreakefieldlentivirus vector expressing membrane-bound Gaussia luciferase with a biotinylation signal both external to the membrane with an IRES GFPBreakefield@hms.harvard.edu
4
BreakefieldBreakefieldYlentivirus vector expressing humanized biotin ligase with a signal sequence -IRES-mCherrylabels all membranes in cells and membranes of EVs; when used in combination with the GlucB vector, the cells biotinylate the protein on the exterior labelling with streptavidin conjugates surface of the membrane allowing sensitive…Breakefield@hms.harvard.eduPMC393450
5
BreakefieldCharest DOX inducible lentivirus expressing palm GFP IRES Cre or Blastlabels plasma membrane and EVs upon dox administration; works in vitro and in vivoacharest@bidmc.harvard.edu
6
BreakefieldCharest floxed F. luciferase transgenic mouseacharest@bidmc.harvard.edu
7
BreakefieldCharest floxed EGFR and PDGFRa transgenic animals and their derivative glioblastoma cell linesacharest@bidmc.harvard.edu
8
BreakefieldCharest DOX inducible lentivirus expressing miRNAs along with Cre or Blastexpress miRNA of choice upon dox administration; works in vitro and in vivoacharest@bidmc.harvard.edu
9
BreakefieldKrichesvkyYglioblastoma exosome-associated small exRNA datasets RNAseq libraries of exosomal RNA, including miRNA and other small RNA species; available within the publicly released exRNA Atlasakrichevsky@rics.bwh.harvard.edu
10
BreakefieldKrichesvky2016-04-16FBS associated small RNA datasetsRNAseq libraries of exRNA found in commonly utilized FBS, including miRNA and other small RNA species akrichevsky@rics.bwh.harvard.edu27503761
11
BreakefieldKrichesvky2016-05-16glioblastoma RNP-associated small exRNA datasets includes miRNA and other small RNA species akrichevsky@rics.bwh.harvard.edu
12
BreakefieldKrichesvky2016-05-16glioblastoma extracellular long RNA datasets includes mRNA and lncRNAakrichevsky@rics.bwh.harvard.edu
13
BreakefieldGould Y; from SBIpFF3Alix KO plasmidCas9-GFP-TK-Puro plasmid for mutating human Alix genesgould@jhmi.edu
14
BreakefieldGould Y; from SBIpFF4CD63 KO plasmidCas9-GFP-TK-Puro plasmid for mutating human CD63 genesgould@jhmi.edu
15
BreakefieldGould Y; from SBIpJM1084CD9 KO plasmidCas9-GFP-TK-Puro plasmid for mutating human CD9 genesgould@jhmi.edu
16
BreakefieldGould Y; from SBIpJM1083HRS KO plasmidCas9-GFP-TK-Puro plasmid for mutating human HRS genesgould@jhmi.edu
17
BreakefieldGould Y; from SBIpJM1086CD81 KO plasmidCas9-GFP-TK-Puro plasmid for mutating human CD81 genesgould@jhmi.edu
18
BreakefieldGould Y; from SBIpJM1087syntenin KO plasmidCas9-GFP-TK-Puro plasmid for mutating human syntenin genesgould@jhmi.edu
19
BreakefieldGould YpJM920CMV-Acyl-mNeonGreen-2a-bleoFor expressing NeonGreen in microvesicles under bleomycin selectionsgould@jhmi.edu
20
BreakefieldGould YpJM921CMV-AcylTyA-mNeonGreen-2a-bleoFor expressing NeonGreen in exosomes under bleomycin selectionsgould@jhmi.edu
21
BreakefieldGould YpJM926CMV-Acyl-mNeonGreen-2a-bsdFor expressing NeonGreen in microvesicles under blasticidin selectionsgould@jhmi.edu
22
BreakefieldGould YpJM927CMV-AcylTyA-mNeonGreen-2a-bsdFor expressing NeonGreen in exosomes under blasticidin selectionsgould@jhmi.edu
23
BreakefieldGould YpJM932CMV-Acyl-mNeonGreen-2a-hygFor expressing NeonGreen in microvesicles under hygromycin selectionsgould@jhmi.edu
24
BreakefieldGould YpJM933CMV-AcylTyA-mNeonGreen-2a-hygFor expressing NeonGreen in exosomes under hygromycin selectionsgould@jhmi.edu
25
BreakefieldGould YpJM938CMV-Acyl-mNeonGreen-2a-puroFor expressing NeonGreen in microvesicles under puromycin selectionsgould@jhmi.edu
26
BreakefieldGould YpJM939CMV-AcylTyA-mNeonGreen-2a-puroFor expressing NeonGreen in exosomes under puromycin selectionsgould@jhmi.edu
27
BreakefieldGould YpJM1010CMV-Acyl-EGFP-2a-bleoFor expressing EGFP in microvesicles under bleomycin selectionsgould@jhmi.edu
28
BreakefieldGould YpJM1019CMV-AcylTyA-EGFP-2a-bleoFor expressing EGFP in exosomes under bleomycin selectionsgould@jhmi.edu
29
BreakefieldGould YpJM1014CMV-Acyl-EGFP-2a-hygFor expressing EGFP in microvesicles under hygromycin selectionsgould@jhmi.edu
30
BreakefieldGould YpJM1020CMV-AcylTyA-EGFP-2a-hygFor expressing EGFP in exosomes under hygromycin selectionsgould@jhmi.edu
31
BreakefieldGould YpJM1003CMV-Acyl-EGFP-2a-puroFor expressing EGFP in microvesicles under puromycin selectionsgould@jhmi.edu
32
BreakefieldGould YpJM1021CMV-AcylTyA-EGFP-2a-puroFor expressing EGFP in exosomes under puromycin selectionsgould@jhmi.edu
33
BreakefieldGould YpJM903CMV-Acyl-tdTomato*-2a-bleoFor expressing tdTomato in microvesicles under bleomycin selectionsgould@jhmi.edu
34
BreakefieldGould YpJM917CMV-AcylTyA-tdTomato*-2a-bleoFor expressing tdTomato in exosomes under bleomycin selectionsgould@jhmi.edu
35
BreakefieldGould YpJM907CMV-Acyl-tdTomato*-2a-bsdFor expressing tdTomato in microvesicles under blasticidin selectionsgould@jhmi.edu
36
BreakefieldGould YpJM923CMV-AcylTyA-tdTomato*-2a-bsdFor expressing tdTomato in exosomes under blasticidin selectionsgould@jhmi.edu
37
BreakefieldGould YpJM911CMV-Acyl-tdTomato*-2a-hygFor expressing tdTomato in microvesicles under hygromycin selectionsgould@jhmi.edu
38
BreakefieldGould YpJM929CMV-AcylTyA-tdTomato*-2a-hygFor expressing tdTomato in exosomes under hygromycin selectionsgould@jhmi.edu
39
BreakefieldGould YpJM915CMV-Acyl-tdTomato*-2a-puroFor expressing tdTomato in microvesicles under puromycin selectionsgould@jhmi.edu
40
BreakefieldGould YpJM935CMV-AcylTyA-tdTomato*-2a-puroFor expressing tdTomato in exosomes under puromycin selectionsgould@jhmi.edu
41
BreakefieldGould YpFF9CAG-lox-tdTomato-lox-AcylTyA-mNeonGreen-2a-NLSmTagBFP2-2a-puroFor expressing tdTomato prior to Cre expression, and exosomal NeonGreen (and nuclear BFP2) after Cre expressionsgould@jhmi.edu
42
BreakefieldGould YpFF9/attBattBattB-CAG-lox-tdTomato-lox-AcylTyA-mNeonGreen-2a-NLSmTagBFP2-2a-puro-attBFor making tranagenic mice with the following inserted at the Rosa26 locus: CAG-lox-tdTomato-lox, followed by exosomal mNeonGreen and nuclear mTagBFP2sgould@jhmi.edu
43
BreakefieldGould YpJM770-pJM900more than 100 plasmids designed to target selected mRNAs to exosomes and/or microvesiclesFor selective targeting of mRNAs to exosomessgould@jhmi.edu
44
BreakefieldGould Y; from SBIHEK293 cell lines deleted for CD63 (verified by mutation screening and loss of protein by IB)human cell line lacking CD63; currently being used to assess the role of CD63 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
45
BreakefieldGould Y; from SBI293T cell lines deleted for CD63 (verified by mutation screening and loss of protein by IB)Currently being used to assess the role of CD63 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
46
BreakefieldGould Y; from SBIHEK293 cell lines deleted for CD9 (verified by mutation screening and loss of protein by IB)human cell line lacking CD9; currently being used to assess the role of CD9 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
47
BreakefieldGould Y; from SBI293T cell lines deleted for CD9 (verified by mutation screening and loss of protein by IB)human cell line lacking CD9; currently being used to assess the role of CD9 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
48
BreakefieldGould Y; from SBIHEK293 cell lines deleted for CD81 (verified by mutation screening and loss of protein by IB)human cell line lacking CD81; currently being used to assess the role of CD81 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
49
BreakefieldGould Y; from SBI293T cell lines deleted for CD81 (verified by mutation screening and loss of protein by IB)human cell line lacking CD81; currently being used to assess the role of CD81 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
50
BreakefieldGould Y; from SBIHEK293 cell lines deleted for CD9 and CD81 (verified by mutation screening and loss of protein by IB)human cell line lacking CD9 and CD81; currently being used to assess the role of CD9 and CD81 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
51
BreakefieldGould Y; from SBIHEK293 cell lines deleted for Alix (verified by mutation screening and loss of protein by IB)human cell line lacking Alix; currently being used to assess the role of Alix in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
52
BreakefieldGould Y; from SBI293T cell lines deleted for Alix (verified by mutation screening and loss of protein by IB)human cell line lacking Alix; currently being used to assess the role of Alix in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
53
BreakefieldGould Y; from SBIHEK293 cell lines deleted for HRS (verified by mutation screening and loss of protein by IB)human cell line lacking HRS; currently being used to assess the role of HRS in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
54
BreakefieldGould Y; from SBI293T cell lines deleted for HRS (verified by mutation screening and loss of protein by IB)human cell line lacking HRS; currently being used to assess the role of HRS in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
55
BreakefieldGould Y; from SBIHEK293 cell lines deleted for Rab35 (verified by mutation screening and loss of protein by IB)human cell line lacking Rab35; currently being used to assess the role of Rab35 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
56
BreakefieldGould Y; from SBI293T cell lines deleted for Rab35 (verified by mutation screening and loss of protein by IB)human cell line lacking Rab35; currently being used to assess the role of Rab35 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
57
BreakefieldGould Y; from SBIHEK293 cell lines deleted for ARF6 (verified by mutation screening and loss of protein by IB)human cell line lacking ARF6; currently being used to assess the role of ARF6 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
58
BreakefieldGould Y; from SBI293T cell lines deleted for ARF6 (verified by mutation screening and loss of protein by IB)human cell line lacking ARF6; currently being used to assess the role of ARF6 in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
59
BreakefieldGould Y; from SBIHEK293 cell lines deleted for Rab11a (verified by mutation screening and loss of protein by IB)human cell line lacking Rab11a; currently being used to assess the role of Rab11a in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
60
BreakefieldGould Y; from SBI293T cell lines deleted for Rab11a (verified by mutation screening and loss of protein by IB)human cell line lacking Rab11a; currently being used to assess the role of Rab11a in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
61
BreakefieldGould Y; from SBIHEK293 cell lines deleted for Rab11a and Rab11b (verified by mutation screening and loss of protein by IB)human cell line lacking Rab11a and Rab11b; currently being used to assess the role of Rab11a and Rab11b in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
62
BreakefieldGould Y; from SBI293T cell lines deleted for Rab11a and Rab11b (verified by mutation screening and loss of protein by IB)human cell line lacking Rab11a and Rab11b; currently being used to assess the role of Rab11a and Rab11b in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
63
BreakefieldGould Y; from SBIHEK293 cell lines deleted for syntenin (verified by mutation screening and loss of protein by IB)human cell line lacking syntenin; currently being used to assess the role of syntenin proteins in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
64
BreakefieldGould Y; from SBI293T cell lines deleted for syntenin (verified by mutation screening and loss of protein by IB)human cell line lacking syntenin; currently being used to assess the role of syntenin proteins in the biogenesis of EVs, and in the biogenesis of exRNA (collaboration with Balaj & Breakefield)sgould@jhmi.edu
65
BreakefieldGould not yet availableb6 mouse with the following integrated at the Rosa26 locus: CAG-lox-tdTomato-lox-AcylTyA-mNeonGreen-2a-NLSmTagBFP2-2a-puroCre-responsive mouse (b6) that (a) expresses cytoplasmic tdTomato in all cells, and (b) switches to exosomal NeonGreen (and nuclear blue expression) after Cre expressionFor generating a map of exosome traffic in the mouse; map will be generated by a combination of (i) transplant studies and (ii) Cre activation studies, with the goal of identifying a map of exosome trafficking in the bodysgould@jhmi.edu
66
McManusMcManusYSensor cell constructs that report on exRNA activitySensor constructs are in the form of lentiviruses and can detect a number of different microRNAs in cell lines. IDG cooperative members are welcome to request vectors as libraries or titered lentivirus. Information related to vectors is available upon request.Michael.McManus@ucsf.eduunpublished
67
McManusMcManusYCRISPR constructsIDG cooperative members are welcome to request vectors as libraries or titered lentivirus. Information related to vectors is available upon request.Michael.McManus@ucsf.eduunpublished
68
McManusMcManusYSensor cell lines that report on exRNA activityMichael.McManus@ucsf.eduunpublished
69
McManusMcManusYsome CRISPR transgenic cell linesMichael.McManus@ucsf.eduunpublished
70
McManusMcManusYexRNA KO miceWe have excellent protocols and can make the mice for you. Mouse reagents can be provided by shipping animals or ES cells or possibly sperm.Michael.McManus@ucsf.eduunpublished
71
McManusMcManusYHigh titer lentivirusWe can make high titer lentivirus in our Core.Michael.McManus@ucsf.eduunpublished
72
McManusMcManusYa number of different microRNA KO miceMouse reagents can be provided by shipping animals or ES cells or possibly sperm.Michael.McManus@ucsf.eduunpublished
73
McManusMcManusYPooled whole genome human shRNA library.Libraries are broken up into subpools. For RNAi, 25-30 shRNA against every Refseq human gene. 600K shRNAs total.Michael.McManus@ucsf.eduunpublished
74
McManusMcManusYPooled whole mouse and human genome CRISPR libraries for editing, CRISPRi, and CRISPRa.Libraries are broken up into subpools. For sgRNAs, 12 sgRNAs/gene for every Refseq gene.Michael.McManus@ucsf.eduunpublished
75
McManusVoinnethappy to share reagents via collaboration (will evolve with publication status)miRNA sensor cell line in a miRNA WT or KO context (using McManus pOSIsensor system)The pOSI-sensor lines were generated from different parental cell lines including 293T (embryonic carcinoma), RKO (colon cancer) and C2C12 (mouse myoblast). So far, we have mostly developed sensors against miR-21 and miR-22. pOSI-sensors are currently being introduced in other cell types.bogdanm@ethz.chunpublished
76
McManusVoinnethappy to share reagents via collaboration (will evolve with publication status)Cell lines stably expressing RNA silencing factors (e.g. Dicer, Drosha, AGO2...)Concerning the RNA silencing factors: we have generated a collection of plasmids coding for either N- or C-terminally tagged DGCR8, DROSHA, XPO5, DICER1, TARBP2, PACT, AGO1, and AGO2. The tag consists of flag-SBP-mNeonGreen. Red (mRuby2) or Blue (mTAGBF2) N-terminal tags are also available. Flag and SBP can be used as affinity tags for immunoprecipitation (e.g. IP-MS). The plasmid backbone is pCDNA5/FRT/TO, allowing easy generation of stable isogenic cell lines using the Flp-in system (Invitrogen). We have already generated stable lines in different parental cell lines including 293T, DLD1, C2C12, Neuro2A, and U2OS.bogdanm@ethz.chunpublished
77
McManusVoinnethappy to share reagents via collaboration (will evolve with publication status) Cell lines allowing a reversible association of tagged RNA silencing factors with specific endomembrane compartments (ER, Golgi, MVB)Concerning the RNA silencing factors: we have generated a collection of plasmids coding for either N- or C-terminally tagged DGCR8, DROSHA, XPO5, DICER1, TARBP2, PACT, AGO1, and AGO2. The tag consists of flag-SBP-mNeonGreen. Red (mRuby2) or Blue (mTAGBF2) N-terminal tags are also available. Flag and SBP can be used as affinity tags for immunoprecipitation (e.g. IP-MS). The plasmid backbone is pCDNA5/FRT/TO, allowing easy generation of stable isogenic cell lines using the Flp-in system (Invitrogen). We have already generated stable lines in different parental cell lines including 293T, DLD1, C2C12, Neuro2A, and U2OS.bogdanm@ethz.chunpublished
78
McManusVoinnethappy to share reagents via collaboration (will evolve with publication status)Mouse models allowing cell-type specific expression of GFP-AGO2 or membrane-bound fluorescent protein in mammary gland (e.g. milk secretion) or intestine cells.These mice make it possible to track AGO2 or vesicles secreted from these two differents organs. bogdanm@ethz.chunpublished
79
McManusHunter happy to share reagents via collaboration (will evolve with publication status)SidT1 and SidT2 KO miceCraig_Hunter@harvard.edu
80
TuschlTuschlexRNA and exDNA isolation protocols including formulations of all individual components for urine/plasma/serumPatented process to fractionate exRNA and exDNA using manuel or robotic process starting with 500 µl biofluid and recovering near 10 ng nucleic acid in 15 µl water in high purity suitable for enzymatic cDNA library preparation; processing of up to 200 samples/day is possible, method tested on about 1000 samples of urine and plasmaDetailed protocols are availabe, but they have not been posted on public web sites to not compromise publication. We are preparing a manuscript to be submitted soonKlaas Max: Klaas.Max@rockefeller.edu; Thomas Tuschl: ttuschl@rockefeller.edu
81
TuschlTuschlSmall RNA cDNA library preparation protocols for parallel processing of 24 samples with 5 ng input exRNA followed by Illumina 50 nt single end sequencingProcess originally developed for miRNA discovery and dependent on 5' phophate/3' hydroxyl termini. Provides 10-100-fold enrichment for RNA with such termini (miRNAs, 5' ends of tRNAs and Y RNAs). Used pre-adenylated bar-coded 3' adapter ligation to initaite sample processing, subsequent pooling, 5' adapter ligation, size fractionation, then cDNA synthesis, PCR and sequencing of barcoded 24 samples per lane.Detailed protocols are availabe, but the latest addition of barcoded adapters has not been published. Hafner M, Renwick N, Farazi TA, Mihailović A, Pena JT, Tuschl T, Barcoded cDNA library preparation for small RNA profiling by next-generation sequencing, Methods. 2012, 58, 164-70. This paper describes a set of 20 adapters and uses larger quantities of input RNA; updates to the protocol will be published with the RNA/DNA isolation manuscript in March 2016Klaas Max: Klaas.Max@rockefeller.edu; Thomas Tuschl: ttuschl@rockefeller.edu
82
TuschlTuschlComputational analysis of small RNAseq data using hierarchical mapping against a curated coding and ncRNA human transcriptomeThese protocols have not been fully tested, they are under development. No manuscript yet in preparationKlaas Max: Klaas.Max@rockefeller.edu; Thomas Tuschl: ttuschl@rockefeller.edu
83
TuschlBeatrice Goilav, Zev Williams Collection of cell-free blood and urine samples from people of lupus subtypes (nephritis, erythematosus) and of normal controls. Immortalized B-cell lines from lupus patientsProtocols for standardized biofluid collections. Protocols for Epstein Barr-Virus-based immortalization of leukocytesSelf-explanatory. Details best discussed in person.bgoilav@montefiore.org, zwilliams@rockefeller.edu
84
TuschlDinshaw PatelStructural determination of protein-nucleic acid complexes by X-ray crystallogrphay and molecular characteriation of protein-nucleic acid complexes using biophysical methodsStructural characterization of riboswitches and DNA- and RNA-sensing innate immunity proteins; no specific approaches for exRNA transport or biogenesisSelf-explanatory. Details best discussed in person.pateld@MSKCC.ORG
85
BlellochMark Anselhappy to share via collaboration
Ago1/Ago2 conditional mutant mice (Eif2c1tm1a(KOMP)Wtsi x Eif2c2tm1.1Tara)
Ago1 allele generated using the KOMP/EUCOMM targeting strategy described as knockout-first allele: Promoterless selection cassette.
http://www.knockoutmouse.org/about/targeting-strategies. Live mouse colony currently in stock and intercrossed with Ago2fl/fl allele from the Tarakhovsky group at The Rockefeller University. 		Mark.Ansel@ucsf.edu
Bronevetsky Y et al. J Exp Med 210(2):417-32 PMID: 23382546
86
BlellochMark Anselhappy to share via collaboration
Conditional epitope tagged Ago2 transgenic mice (Gt(ROSA)26Sortm1(CAG-GFP/Eif2c2)Zjh) crossed to Ago2 conditional knockout mice
Previously published transgenic mouse bearing floxed STOP, GFP and myc tagged Ago2 under the control of the CAG promoter in the ROSA26 locus. Cre-mediated removal of a floxed STOP sequence allows expression of the GFP/myc tagged Ago2, with GFP expression indicating expression of Ago2 and the myc epitope tag allowing affinity purification of associated miRNAs. Live mouse colony currently in stock and intercrossed with Ago2fl/fl allele from the Tarakhovsky group at The Rockefeller University.
Mark.Ansel@ucsf.edu
unpublished intercross. Original mice in PMID 22243745 and PMID 17626790
87
BlellochRobert Blellochhappy to share via collaborationRWPE myr-AKT (untransformed prostate epithelial line overexpressing myr-AKT)Oncogene introduced as a retrovirus and purified by puro selection.Robert.Blelloch@ucsf.eduunpublished
88
BlellochRobert Blellochhappy to share via collaborationRWPE HRasV12 (untransformed prostate epithelial line overexpressing HRas V12)Oncogene introduced as a retrovirus and purified by puro selection.Robert.Blelloch@ucsf.eduunpublished
89
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A MYC 5X cells (human MYC over-expression ~ 5-fold by western blot)Andrei.Goga@ucsf.eduPMCID: PMC4407699
90
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A myr-AKT (cells over-expressing myr-AKT)Andrei.Goga@ucsf.edu
PMCID: PMC4407699
91
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A AURKB (cells stably over-expressing Aurora Kinas B)Andrei.Goga@ucsf.edu
PMCID: PMC4407699
92
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A HRAS V12 expressionAndrei.Goga@ucsf.edu
PMCID: PMC4407699
93
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A activated beta-catenin stable expressionAndrei.Goga@ucsf.edu
PMCID: PMC4407699
94
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A NOTCH (activated Notch intracellular domain overexpression)Andrei.Goga@ucsf.edu
PMCID: PMC4407699
95
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A Puro / Blast control cellsAndrei.Goga@ucsf.edu
PMCID: PMC4407699
96
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A Src-ER (conditional SRC activation)Andrei.Goga@ucsf.edu
PMCID: PMC4407699
97
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A BRAF V600E activated alleleAndrei.Goga@ucsf.edu
PMCID: PMC4407699
98
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A PIK3CA activated allele stabkle over-expressionAndrei.Goga@ucsf.edu
PMCID: PMC4407699
99
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A CCND1 OverexpressionAndrei.Goga@ucsf.edu
PMCID: PMC4407699
100
BlellochAndrei Gogacells happy to share; gene expression data happy to share via collaborationMCF10A NeuNT stable overexpressionAndrei.Goga@ucsf.edu
PMCID: PMC4407699
Loading...
 
 
 
U19 Reagents
U19 Methods
 
 
Main menu