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Research in Diagnostics - Collection
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A combined effort of the Open Bioeconomy Lab at University of Cambridge (Jenny Molloy, Chiara Gandini), the Lab de tecnologías Libres at iBio/PUC Chile (Fernan Federici, Isaac Núñez, Tamara Matute, Anibal Arce), the Free Genes Project (Isaac Larkin) and Open Science Network Society (Scott Pownall)
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This file has been updated with information available on 14 Aug 2020
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The available DNA parts can be pre ordered from FreeGenes here. The here listed DNA parts are distribued under the FreeGenes collections "Molecular diagnostic toolkit" and "E. coli protein expression toolkit"
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This collection would like to enable researchers worldwide to express the enzymes useful to undertake research in diagnostic, at the time of a global pandemic and supply chain disruptions.
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We are workign together with Ginkgo Bioworks to make this collection a reality.To know more about the initiative, read the signed Letter to Ginkgo here
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The collection wants to provide researchers with:
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1) a set of ready-to-use expression vectors for E. coli: express and purify the enzymes of your choice that can be used to undertake research in diagnostic (e.g. RT, PCR, LAMP, RPA)
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2) a set of DNA parts ready to be assembled using MoClo standards, to enbable researchers to create expression vectors tailored to the resources available in their own lab
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Note: all the DNA sequences are off-patent and distributed under OpenMTA
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Sheets
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Schematic assembly of DNA parts using MoClo syntax is now color coded referring to parts availability in 14 August 2020
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Expression Cassettes is an updated list of expression cassettes that includes information on DNA parts availability in 14 Aug 2020
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Note: Parts not yet available did fail synthesis at Twist, we are working to make them available as soon as possible
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