Guide toOBC microscopes and general Cellular Structure-Specific Dyes and Probes: Compatibility, Excitation, and Imaging Techniques

	A	B	C	D	E	F
1	Microscope Model	Lasers (nm) and SpectraViewer	Detection Systems	Additional Capabilities	Live Imaging Capabilities	Ideal for

2	Zeiss Z1 Lightsheet	405, 488, 561, 631	2 Cameras	Additonal Mesoscale chamber by Translucence Bio for organically cleared samples.	YES	Volumetric imaging with minimal phototoxicity; cleared tissue samples. Has multiple imaging chambers for organice and water
3	Elyra 7 Lattice SIM	405, 488, 561, 633	2 Cameras	Super-Resolution down to 60nm with Lattice SIM mode, 20nm with SMLM. TIRF	YES	Super-resolution imaging; Structures near or below the diffraction limit
4	LSM 780 Confocal	405, 458, 488, 514, 561, 633	32 Channel Spectral Detectors 2 PMTs	Two-photon microscopy	YES	High-resolution imaging; Fluorescence spectral unmixing; Deep tissue penetration
5	LSM 980 with Airyscan 2	405, 488, 561, 633	32 Channel Spectral Detector, 2 GaAsp detectors, 1 Aiyrscan , 2 NDDs	Airyscan 2 for enhanced resolution and sensitivity	YES	High-sensitivity and super-resolution; Quantitative imaging with spectral unmixing
6	LSM 900 with Airyscan 2	405, 488, 561, 633	2 GaAsp Detectors, 1 Airyscan detector	Airyscan 2 for enhanced resolution and sensitivity. 2NDD detectors for improved image quality and increased signal intensity from deep within specimens	NO	Super-resolution imaging; High-sensitivity detection in live and fixed samples
7	Leica Sp8 Confocal	405, 458, 488, 514,561, 633	4 Detectors (2 PMTs, 2 HyDs)	4 detectors for fast multicolor imaging	NO	Flexible imaging with high sensitivity; Suitable for a wide range of fluorescence applications
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