ABCDEFGHIJKLMNOPQRSTUVWXYZAAABACADAEAFAG
1
ttitles and junk
2
17/Dec/24
q63_S099_gfp splice repeat
4ul template10 ul reactionPK67rxn total
qPCRBIO SyGreen 1-Step Detect
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
3
<>123456789101112
Target/primer pair
Sample categoryAssay variableLabel_finalFunctionformula (add '=')
4
FRP nameAfresh-pBBR1_spliced_311
old-cloDF_spliced_186
fresh-pBBR1_GFP unspl._311
old-cloDF_GFP unspl._186
fresh-pBBR1_Ribo unspl._311
old-cloDF_Ribo unspl._186
old-cloDF_spliced_C+-89
old-cloDF_Ribo unspl._C+-89
old-cloDFsplicedC+-89old-cloDF_spliced_C+-89Letter additionCHAR(code(T219) + 1)
Pos-gfp-/103_1.1
gfpTest_89.1
5
Bfresh-pBBR1_spliced_311
old-cloDF_spliced_186
fresh-pBBR1_GFP unspl._311
old-cloDF_GFP unspl._186
fresh-pBBR1_Ribo unspl._311
old-cloDF_Ribo unspl._186
old-cloDF_spliced_C+-89
old-cloDF_Ribo unspl._C+-89
old-cloDFsplicedC+-89old-cloDF_spliced_C+-89Letter and number
CHAR(code(R341) + 1) & "." & 1
Pos-gfptest/186_1.1
16sU64Test_295.1
6
Cfresh-pBBR1_spliced_311
old-cloDF_spliced_186
fresh-pBBR1_GFP unspl._311
old-cloDF_GFP unspl._186
fresh-pBBR1_Ribo unspl._311
old-cloDF_Ribo unspl._186
old-cloDF_spliced_C+-89
old-cloDF_Ribo unspl._C+-89
old-cloDFsplicedC+-89old-cloDF_spliced_C+-89
Pos-gfp+/89_1.1
16sU1Test_297.1
7
D
fresh-pBBR1_spliced_C-314
old-cloDF_spliced_C-103
fresh-pBBR1_GFP unspl._C-314
old-cloDF_GFP unspl._C-103
fresh-pBBR1_Ribo unspl._C-314
old-cloDF_Ribo unspl._C-103
old-cloDF_spliced_ntc
old-cloDF_Ribo unspl._ntc
old-cloDFsplicedntcold-cloDF_spliced_ntc
Pos-16sU64/295_1.1
16sU8Test_298.1
8
Flags belowE
fresh-pBBR1_spliced_C-314
old-cloDF_spliced_C-103
fresh-pBBR1_GFP unspl._C-314
old-cloDF_GFP unspl._C-103
fresh-pBBR1_Ribo unspl._C-314
old-cloDF_Ribo unspl._C-103
old-cloDF_GFP unspl._C+-89
old-cloDFsplicedntcold-cloDF_spliced_ntc
Pos-16sU1/297_1.1
16sU34Test_299.1
9
F
fresh-pBBR1_spliced_C-314
old-cloDF_spliced_C-103
fresh-pBBR1_GFP unspl._C-314
old-cloDF_GFP unspl._C-103
fresh-pBBR1_Ribo unspl._C-314
old-cloDF_Ribo unspl._C-103
old-cloDF_GFP unspl._C+-89
old-cloDFsplicedntcold-cloDF_spliced_ntc
Pos-16sU8/298_1.1
16sU73Test_300.1
10
Gfresh-pBBR1_spliced_ntc
old-cloDF_spliced_ntc
fresh-pBBR1_GFP unspl._ntc
old-cloDF_GFP unspl._ntc
fresh-pBBR1_Ribo unspl._ntc
old-cloDF_Ribo unspl._ntc
old-cloDF_GFP unspl._C+-89
old-cloDFsplicedntcold-cloDF_spliced_ntc
Pos-16sU34/299_1.1
old16sU64Test_295.1
11
Hbad-pBBR1_spliced_311
bad-pBBR1_spliced_311
bad-pBBR1_spliced_311
bad-pBBR1_GFP unspl._311
bad-pBBR1_GFP unspl._311
bad-pBBR1_GFP unspl._311
bad-pBBR1_Ribo unspl._311
bad-pBBR1_Ribo unspl._311
bad-pBBR1_Ribo unspl._311
old-cloDF_GFP unspl._ntc
bad-pBBR1spliced311bad-pBBR1_spliced_311
Pos-16sU73/300_1.1
old16sU1Test_297.1
12
13
12/Nov/24
q62_S097b_rpoB_v2v3-2_Makenzi
4ul template10 ul reactionPK72rxn total
qPCRBIO SyGreen 1-Step Detect
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
23
24
12/Nov/24
q59b_hamilton test2_std59
4ul template10 ul reactionPK46rxn total
qPCRBIO SyGreen 1-Step Detect
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
34
35
24/Oct/24
q61_S097_rpoB_v2v3_test
4ul template10 ul reactionPK58rxn total
qPCRBIO SyGreen 1-Step Detect
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
45
46
23/Oct/24
q60_S094b_mScarlet stability check
4ul template10 ul reactionPK64rxn total
qPCRBIO SyGreen 1-Step Detect
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
56
57
5/Aug/24
q58_S093-RAM spiked qPCR3
4ul template10 ul reactionPK32rxn total
qPCRbio probe 1-step Go
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
67
68
2/August/24
q57_S093-RAM test qPCR2
4ul template10 ul reactionPK32rxn total
qPCRbio probe 1-step Go
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
78
79
23/July/24q56_S093_RAM test 14ul template10 ul reactionPK32rxn total
qPCRbio probe 1-step Go
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
89
90
13/June/24
q55_S093_Shyams leak mSc
4ul template22 ul reactionPK32rxn total
qPCRBIO SyGreen 1-Step Detect
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
100
101
25/May/24
S091b_marine 4,5-ddPCR
4ul template22 ul reactionPK96rxn totalddPCR no dUTPTemplate type =
HindIII dig Lysate (1e4-1e3;10x), MB, ytss cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
102
<>123456789101112
Target/primer pair
Sample categoryAssay variableLabel_finalFunctionformula (add '=')
103
FRP nameADs_J23100Rd_J23100
Ds_high-growth
Ds_highDs_highRd_J23100Rp_highDs_lowDs_WTDs_WTDs_WT
Ds_low-growth
DsWTDs_WTLetter additionCHAR(code(T219) + 1)
Pos-gfp-/103_1.1
gfpTest_89.1
104
16S duxBDs_J23100Pi_J23100
Rp_med-growth
Rp_medRp_medRd_J23100Rd_highRp_lowRp_WTRp_WTRp_WT
Rp_low-growth
RpWTRp_WTLetter and number
CHAR(code(R341) + 1) & "." & 1
Pos-gfptest/186_1.1
16sU64Test_295.1
105
CDs_J23100Pi_J23100
Rp_high-growth
Rp_highRp_highRd_J23100
Negative_NTC
Ds_lowRd_WTRd_WTRd_WT
Rd_low-growth
RdWTRd_WT
Pos-gfp+/89_1.1
16sU1Test_297.1
106
Flags belowDRp_J23100Pi_J23100
Rd_high-growth
Rd_highRd_high
Rp-ytss_J23100
Negative_blank
Rp_lowPi_WTPi_WTPi_WT
Pi_low-growth
PiWTPi_WT
Pos-16sU64/295_1.1
16sU8Test_298.1
107
ERp_J23100
Rp-ytss_J23100
Pi_high-growth
Pi_highPi_high
Negative_NTC
Negative_NTC
Negative_NTC
Rp-ytss_WTRp-ytss_WTRp-ytss_WT
Rp-ytss_low-growth
Rp-ytssWTRp-ytss_WT
Pos-16sU1/297_1.1
16sU34Test_299.1
108
FRp_J23100
Rp-ytss_J23100
Rp-ytss_med-growth
Rp-ytss_medRp-ytss_med
Negative_blank
Ds_WT
Negative_blank
Rd_lowRd_low
Negative_blank
Negative_blank
NegativeblankNegative_blank
Pos-16sU8/298_1.1
16sU73Test_300.1
109
GRd_J23100
Rp-ytss_J23100
Rp-ytss_high-growth
Rp-ytss_highRp-ytss_high
Ec-hmb_J23100
Pi_WTRd_lowPi_lowPi_lowEc-lb_WTEc-hmb_WTEc-hmbWTEc-hmb_WT
Pos-16sU34/299_1.1
old16sU64Test_295.1
110
HRd_J23100
Negative_blank
Negative_blank
Ec-lb_J23100Ec-lb_J23100Ec-lb_J23100Rp-ytss_WTPi_lowRp-ytss_lowRp-ytss_lowEc-lb_WTEc-lb_WTEc-lbWTEc-lb_WT
Pos-16sU73/300_1.1
old16sU1Test_297.1
111
Strip marker11, e412, e413, e33.[1-7], e13.[1-7], e1sel 11,12. e3
selected. e1/ 10 ul templ
4.7,8 e114.[1-5], e414.[1-5], e414, e415, e3
112
5/May/24S091_marine 4-ddPCR4ul template22 ul reactionPK40rxn totalddPCR no dUTPTemplate type =
HindII dig Lysate (1e5-10x), MB, ytss cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
113
<>12345
Target/primer pair
Sample categoryAssay variableLabel_finalFunctionformula (add '=')
114
FRP nameARd_J23100
Negative_blank
Negative_blank
Rp_low
Negative_NTC
duplexTravis1duplex_Travis_1Letter additionCHAR(code(T219) + 1)
Pos-gfp-/103_1.1
gfpTest_89.1
115
16S duxBRd_J23100
Rp-ytss_J23100
Rp-ytss_highDs_low
Negative_NTC
duplexTravis2duplex_Travis_2Letter and number
CHAR(code(R341) + 1) & "." & 1
Pos-gfptest/186_1.1
16sU64Test_295.1
116
(Freshly made)CRp_J23100
Rp-ytss_J23100
Rp-ytss_med
Negative_blank
Negative_NTC
duplexTravis3duplex_Travis_3
Pos-gfp+/89_1.1
16sU1Test_297.1
117
Remove duplex from the analysis pipeline? / make it optional
DRp_J23100
Rp-ytss_J23100
Pi_highRp-ytss_WT
Negative_NTC
duplexTravis4duplex_Travis_4
Pos-16sU64/295_1.1
16sU8Test_298.1
118
Unexpected plasmid amplification
ERp_J23100Pi_J23100Rd_highPi_WT
Negative_blank
duplexTravis5duplex_Travis_5
Pos-16sU1/297_1.1
16sU34Test_299.1
119
too few droplets
FDs_J23100Pi_J23100Rp_highRd_WTRp-ytss_lowduplexNegative502duplex_Negative_502
Pos-16sU8/298_1.1
16sU73Test_300.1
120
No amplification
GDs_J23100Pi_J23100Rp_medRp_WTPi_lowduplexNegativeNTCduplex_Negative_NTC
Pos-16sU34/299_1.1
old16sU64Test_295.1
121
Weird clusterHDs_J23100Rd_J23100Ds_highDs_WTRd_lowduplexNegativeNTCduplex_Negative_NTC
Pos-16sU73/300_1.1
old16sU1Test_297.1
122
123
6/Mar/24
q54_S079-RNA2_Travis-1-5_RAM
4ul template22 ul reactionPK32rxn total
qPCRbio probe 1-step Go + qPCRBIO Sygreen Blue mix (not meant for RT)
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
133
134
20/Dec/23
q52_D19-20_new RAM repression_shorter bc
4ul template22 ul reactionPK62rxn total
qPCRbio probe 1-step Go + qPCRBIO Sygreen Blue mix (not meant for RT)
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
144
145
27/May/23
q50_S080-RAM repression 80a, mjd61
4ul template22 ul reactionPK43rxn total
qPCRbio probe 1-step Go + qPCRBIO Sygreen Blue mix (not meant for RT) + qPCRbio RT mix
Template type =
DNAsed RNA extract / 10x dil
Labelling helper
Don't use "_" in assay variable
Formula helper
155
156
27/May/23
S075_1e4 dil marine 16S all
4ul template22 ul reactionPK24rxn totalddPCR no dUTPTemplate type =
Lysate (1e3-4), MB cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
166
167
17/May/23
q49_Std49 triplexes_v23 v0
4ul template22 ul reactionPK52rxn totalddPCR no dUTPTemplate type =
Lysate (1e3-4), MB cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
177
178
2/May/23
S074b_1e4 dil marine 16S all_16-5-23
4ul template22 ul reactionPK16rxn totalddPCR no dUTPTemplate type =
Lysate (1e3-4), MB cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
188
189
2/May/23
S072b_marine 16S hindIII-HF
4ul template22 ul reactionPK8rxn totalddPCR no dUTPTemplate type =
Lysate (1e3-4), MB cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
199
200
26/Apr/23
S072dd_marine 16S tst_26-4-23
4ul template22 ul reactionPK16rxn totalddPCR no dUTPTemplate type =
Lysate (1e3-4), MB cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
210
211
26/Apr/23q48d_S071_d84ul template10 ul reactionPK72rxn totalqPCRbio probeTemplate type =
Lysate (1/5x), M9 cultures spun
Labelling helper
Don't use "_" in assay variable
Formula helper
221
222
26/Apr/23q48c_S071_d24ul template10 ul reactionPK72rxn totalqPCRbio probeTemplate type =
Lysate (1/5x), M9 cultures spun
Labelling helper
Don't use "_" in assay variable
Formula helper
232
233
24/Apr/23S072_16S frp test4ul template10 ul reactionPK8rxn totalqPCRbio probeTemplate type =Lysates, in MBLabelling helper
Don't use "_" in assay variable
Formula helper
243
244
23/Apr/23q48b_S071_d5, -14ul template10 ul reactionPK96rxn totalqPCRbio probeTemplate type =
Lysate (1/5x), M9 cultures spun
Labelling helper
Don't use "_" in assay variable
Formula helper
254
255
22/Apr/23q48a_S071_d0, -14ul template10 ul reactionPK96rxn totalqPCRbio probeTemplate type =
Lysate (1/5x), M9 cultures spun
Labelling helper
Don't use "_" in assay variable
Formula helper
265
266
14/Apr/23q47y_S070_Ara 44ul template10 ul reactionPK52rxn totalqPCRbio probeTemplate type =
Lysate (1/5x), M9 cultures spun
Labelling helper
Don't use "_" in assay variable
Formula helper
276
277
30/Mar/23
q46b_S057j_lysate touchup
4ul template10 ul reactionPK16rxn totalqPCRbio probeTemplate type =
Lysate, M9 cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
287
288
28/Mar/23q45_S067_top3 till d24ul template10 ul reactionPK89rxn totalqPCRbio probeTemplate type =
Lysate, M9 cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
298
299
23/Mar/23
q44_S068 best lysis_S057i marine lysate
4ul template10 ul reactionPK70rxn totalqPCRbio probeTemplate type =
Lysate, M9 cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
309
310
7/Mar/23q43_S065_M9 test4ul template10 ul reactionPK48rxn totalqPCRbio probeTemplate type =
Lysate, M9 cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
320
321
11/Jan/23
q42_wild memory-prelim
4ul template10 ul reactionPK70rxn totalqPCRbio probeTemplate type =
Lysate, M9 cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
331
332
2/Dec/22
q40e_S050_colony-2d -ves
4ul template10 ul reactionPK30rxn totalqPCRbio probeTemplate type =
Lysate, M9 cultures
Labelling helper
Don't use "_" in assay variable
Formula helper
342
343
18/Nov/22
q40d_S050_fresh transformation_-ves_memory
4ul template10 ul reactionPK16rxn totalqPCRbio probeTemplate type =
Plasmids + Lysate, LB culture
Labelling helper
Don't use "_" in assay variable
Formula helper
353
Changed the labelling format above this
354
1/Nov/22
q41_S050_pilot-3_pSS079
4ul template10 ul reactionPK35rxn totalqPCRbio probeTemplate type =
Lysate, M9 cultures
Labelling helper
Don't use "-",".","_" in assay variable
Formula helper
364
365
1/Nov/22
q0401c_S050_followup 3 -ves_memory
4ul template10 ul reactionPK21rxn total
qPCRbio probe 1-step Go
Template type =
Plasmids + Lysate, M9 culture
Labelling helper
Don't use "-",".","_" in assay variable
Formula helper
375
376
29/Oct/22
q040b_S050_re-followup -ves_memory
4ul template10 ul reactionPK14rxn total
qPCRbio probe 1-step Go
Template type =
Lysate, M9 cultures
Labelling helper
Don't use "-",".","_" in assay variable
Formula helper
386
387
28/Oct/22
q40_S050_followup -ves_memory
4ul template10 ul reactionPK8rxn total
qPCRbio probe 1-step Go
Template type =PlasmidsLabelling helper
Don't use "-",".","_" in assay variable
Formula helper
397