RNA Polymerases

	A	B
1	Open Enzyme Collection Registry: RNA Polymerases
2	Project Collaborators: Open Bioeconomy Lab (University of Cambridge), Kumasi Hive Individual Contributors: Jenny Molloy (University of Cambridge), Harry Akligoh (Kumasi Hive), Chiara Gandini (University of Cambridge) Supported by: Shuttleworth Foundation This spreadsheet documents open RNA polymerase sequences whereby the patent covering the enzyme or sequence itself has expired. The uses of that enzyme for molecular biology and biotechnology protocols and applications may still be covered by patents in various countries and we are compiling a list of these separately.
3
4
5
6
7
8	Number of Open Enzymes Synthesised	3
9	Number of Open Enzymes Not Yet Synthesised	0
10	Total Open Enzymes in Collection	3
11
12	Key to column headings
13	DNA Polymerase	Name or commonly used description of the DNA polymerase. Equivalent trademarked names are provided for reference where applicable.
14	Patent Expired	Whether the patent covering the enzyme or its sequence has expired.
15	Features and Applications	Features of the polymerase and its applications in molecular biology and biotechnology
16	Original Paper	The paper where the enzyme is initially described, in some cases this is the original discovery and in some cases the first characterisation of the recombinant form or where possible both cases are provided.
17	Original Patent Family	The first patent family covering the enzyme, which may not include all subsequent modifications.
18	US Filing/Application Date	Priority date for the first US patent covering the enzyme. This is the date at which protection for the invention begins.
19	US Issue Date	Issue date for the first US patent covering the enzyme.
20	US Expiration Date	In the United States, for utility patents filed on or after June 8, 1995, the term of the patent is 20 years from the earliest filing date of the application on which the patent was granted and any prior U.S. or Patent Cooperation Treaty (PCT) applications from which the patent claims priority (excluding provisional applications). For patents filed prior to June 8, 1995, the term of patent is either 20 years from the earliest filing date as above or 17 years from the issue date, whichever is longer. We have used this formula except in cases where i) an extension to term or a termination has been specified in the patent; ii) the patent is listed as expired due to lack of payment of fees.
21	Organism	Organism from which the wild-type sequence was sourced.
22	DNA Sequence
23	Protein Sequence
24	Notes	Any additional notes relevant to the enzyme or patents
25	Twist synthesis score	Is the score that the sequence will reach if submitted to Twist for synthesis. It could be standard, Difficult, Impossible or Too Short (impossible sequences are adjusted to standard e.g. tandem of A are broke changing codon)
26	Internal Project Notes
27	Purification/Affinity Tags Used
28	Notes on Benchling
29
30
31	not in the Open Enzyme collection yet
32	present in the Open Enzyme collection
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100