20131203 - cPCR WS Full Length
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20131203 - cPCR WS Full Length
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Master Mix Calcs
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CiCfVol. (uL)# RxnsTotal Vol (uL)
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Template2
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5x GoTaq Flexi Buffer (Promega)5x1x1011110
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WS_16s_1_F10uM0.5uM2.51127.5
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WS_16s_1501_R10uM0.5uM2.51127.5
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MgCl225mM1.5mM31133
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BSA10mg/mL0.4ug/mL21122
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dNTPs (Bioline)10mM0.2mM11111
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GoTaq Flexi (Promega)5U/uL0.5115.5
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H2O26.511291.5
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Total50Add 48uL to each
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