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Description Registry Name Part Size Vector (size)Plasmid size (vector + part)Origin Marker Transformed?MiniprepGlycerol StockSequenced?DigestPCR
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P1 Low promoter+RBS +GFP+termJ23113/I20259919pSB3K3 (2750)3669p15AKanin S1 and TOP10Yes for both strainsYes for both strains.S1P1A_A, P1E2 (tube labeled E1A)XP (purified)
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P2 Med promoter+RBS+ GFP+termJ23150/I20269919pSB3K3 (2750)3669p15AKanin TOP10, not S1YesP2E2 (tube labeled 2B)
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P3 High promoter+RBS+ GFP+termJ23151/I20270919pSB3K3 (2750)3669p15AKanin TOP10 and S1Yes for both strains.Yes for both strains.P3E2 (tube labeled E3B), S1P3A_A
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P4 Low-Medium copy vectorpACYC duet4008p15aCmin S1 and TOP10Yes, for both strains.Yes for both strains.
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P5 Low-Medium copy vector (w/ death gene)pSB3K32750p15aKanin E3YesYes (B- done 6/30. A-done 7/1)
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P11 Light responsive system, dual regulationBBa_M301094333pSB1AC3 (3055)7388pMB1Amp/CmFailedfailed
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P13 Low copy plasmidpCDFDuet-13781CloDF13Smin S1Yes, 435 ng/uLYesPCR product- XP (purified)yes (with BB ends, purified)
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P15 pTet+RBS+GFP+termBBa_I13522937BBa_J23006 (3279)ColE1Ampin E2YesNoP15A, P15BXP (purified)
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P16 Tet repressible promoterBBa_R004054pMB1Ampin E2YesNo
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P17 Inverter (TetR w/o promoter and Tet promoter)BBa_Q04400902pSB2K3 (4425)5327OriS, P1 lytic, F1Kanin E1BADBADcells from MIT correctES purified, XP purifiedyes (purified)
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P18 cI regulated promoterR005149pMB1Ampin E2YesYes, 7/4P18A, P18B appear so (20 bp align at end, but ref is 49 bp, so this isn't bad)SP (purified), as vector (?)
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P20 GFP (LVA tagged) with Lac promoterBBa_J044311122pMB1Amp, Kanin E2YesNo?! P20A: ~100 bp of beginning of F read aligned to ref; R read did notXP (purified)
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P21 pACYC (P4) w/ biobricksA1 (pACYC)?????????in S1 and TOP10YesYes, for both strains.
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P22 ompR controlled promoter for ompC (long)BBa_R0082108pMB1AmpWRONGWRONGWRONG: DID NOT ALIGN TO REF
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P23 ompR controlled promoter for ompC (short)BBa_R008378pMB1Ampin E2YesNoWRONGWRONGWRONG: DID NOT ALIGN TO REFEX (purified)
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P24 ompR controlled promoter for ompFBBa_R0084108pMB1Ampin E2YesNoWRONGWRONGWRONG: DID NOT ALIGN TO REFEX (purified)
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P25 Promoter activated by OmpR-P with the reporter GFPBBa_J58102991pMB1AmpWRONGWRONGWRONG: DID NOT ALIGN TO REF
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P26 Mutated promoter activated by OmpR-P with the reporter GFPBBa_J58103961pMB1Ampin E2YesNoWRONGWRONGWRONG: DID NOT ALIGN TO REFas insert (?)failed
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P27 repressor plasmid w/ constitutive expression of LacI and TetRpZS4int2pSC101SmYesYes (6/26)Yes (6/26)
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P28 GFP w/ PLtctO-1pZE21ColE1KanYesYes (6/26)Yes (6/26)
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P29 Venus (mutant YFP) PL (Plambda)pZS*2RpSC101*KanYesYes (6/26)Yes (6/26)
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P30 YFP w/ PLlacO-1pZA32p15ACmYesYes (6/26)Yes (6/26)
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P31 Venus w/ PLlacO-1pZS*1RpSC101*AmpYesYes (6/26)Yes (6/26)
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P32 Venus w/ PLlacO-1pZA32p15ACmYesYes (6/26)Yes (6/26)
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P33Venus w/ PLtctO-1pZA31p15ACmYesYes (6/26)Yes (6/26)
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P34 GFP w/ PLlacO-1pZE12ColE1AmpYesYes (6/26)Yes (6/26)
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P35 accessory pLDR8YesYes (6/26)Yes (6/26)
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P36 same as P20BBa_I763004Kan/Amp
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P37 Constructive expression LacI w/ pTetR promoterBBa_I722007AmpFailed (liquid cultures didn't grow)
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P38 High constitutive promoterBBa_J2311435Amp2007 in E1 (DH5a), 2008 Failed, MITYes BUT funny digestion pattern2007 (B- done 6/30. A- done 7/1)SP (purified, but potentially problematic)failed
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P39 Low constitutive promoterBBa_J2311335Amp2008 (A grew), 2007 (A grew), MITYes BUT funny digestion pattern2008 (A- done 6/30) 2007 (A-done 6/30)SP (purified, but potentially problematic)failed
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P40 Ribosome Binding SiteBBa_B003213Amp2008 (A grew), 2007 (B grew)Yes.2007 (B -done 6/30) 2008 (A-done 6/30)SP (purified)
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P41 Transcriptional TerminatorBBa_B0010Amp2007 (A and B grew, 7/2), 2008 (B grew, 7/2)2007 (A and B done 7/4), 2008 (B done 7/4)
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P42 LacI coding regionBBa_C0012
1128AmpFailed (liquid cultures didn't grow)failed
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P43 TetR coding regionBBa_C0040660Amp2007 (A grew)Yes2007 (A-done 6/30)P43A E1 07XP (purified)
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P44 cI lambdaBBa_C0051
750Amp2007 (A grew)Yes2007 (A-done 6/30)P44A E1 07XP (purified)
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P45 GFP only w/ RBS & terminatorBBa_E0240876pSB1A2 (2079)2955pMB1Amp2007 (A and B grew)Yes2007 (A and B -done 6/30)P45A, P45B >E1 07 for bothSP (purified), XP (not purified, potentially problematic, used)
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P46 Base VectorBBa_I51020AmpA and B grew.YesA and B -done 6/30
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P47 Kan Resistance CassetteBBa_P1003967Kan2007 (A and B grew, but spilled)Yes2007 (A and B -done 7/1)P47A, P47B >E1 07 for both
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P48 Cm Resistance CassetteBBa_P1004769pSB1A1 (2708)3477Cm2007 (A and B grew, but spilled)Yes2007 (A and B -done 7/1). Duplicate done 7/4.P48B E1 07XP (purified)
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P49Amp Resistance CassetteBBa_P1002943pSB1A1 (2708)3651Amp2008 (A grew), 2007 (A and B grew)Yes2008 (A-done 6/30) 2007 (A and B -done 6/30)P49a, P49b > both E1 07XP (purified)
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P50 Tet Resistance CassetteBBa_P10051283Tet2007 (A and B grew)Yes2007 (A and B- done 7/1)P50A E1 07, P50B E1
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P51LacI QPI w/ RBS & promoterBBa_Q041211370pSB2K3 (4425)5795pMB1Kancells from MIT correctXP purifiedyes (purified)
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P52cI QPI w/ RBS & promoterBBa_Q04510987pSB2K3 (4425)5412pMB1KanXP purifiedyes (purified, potentially problematic)
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P53PoPS --> TetRBBa_P0440840Amp2007 (B grew), 2008 (A grew)Yes. (7/3).2007 (B done 7/3), 2008 (A done 7/3)ES (purified)
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P54PoPS --> LacIBBa_P04121308Amp2007 (A grew)Yes. (7/3).2007 (A done 7/3)ES (purified, potentially problematic)
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P55RBS.Lambda.cI. LVA.TerminatorBBa_P0455896A/C2007 (A and B grew). 2008 (A grew)Yes for A and B (7/3).2007 (A and B done 7/3). 2008 (A grew)
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P56RBS+TetR coding region (P40 and P43)AmpYes in E1.Yes for A and B (7/3).A and B done 7/3EX (purified), as insert (?), XP (purified)
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P57RBS+lambda cI coding region (P40 and P44)CompositeAmpYes in E1.Yes for A and B (7/3).A and B done 7/3XP (purified), EX (unpurified), as insert (?)
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P58GFP under pTet (with RBS and term) on plasmid with p15A origin (P1 vector + P15 part)Composite3016KanYes in E1.Yes for A and B (7/3).A and B done 7/3EX (purified)
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P59GFP under pLac (with RBS and term) on plasmid with p15A origin (P1 vector + P20 part)Composite3201KanYes in E1.Yes for A and B (7/3).A and B done 7/3EX (purified)
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P60Composite
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P61TerminatorBBa_B00112008 (A grew.)Yes for 2008 A.2008 (A done 7/4)
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P62TerminatorBBa_B00122007 (A and B grew). 2008 ((Plate yielded 1 colony only- failed to grow)Yes for A and B 2007.2007 (A and B done 7/4)
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P63Double terminator (P62 and P61)BBa_B001495pSB1AK3 (3189)3284Amp/Kan2007 (A and B grew). 2008 (Plate yielded 1 colony only- failed to grow)Yes for A and B 2007.2007 (A and B done 7/4)yes with ES; EX
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P64Double terminator (P60 and P62)BBa_B00152007 (A grew)2007 (A done 7/4)
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P65Double terminator (P62 and P60)BBa_B0025Failed (liquid cultures didn't grow)
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P66hi promoter+RBS+TetR coding region (P38+P56)Composite
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P67Low promoter+RBS+TetR Composite
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P68hi promoter +RBS+lambda cI coding region (P38+P57)Composite
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P69Low promoter+RBS+lambda cI repressorComposite
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P70P66 and termComposite
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P71P67 and termComposite
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P72P68 and termComposite
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P73P69 and termComposite
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P74lambda cI promoter+RBS+GFP+term (P45 + P18)CompositeYes.yes
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P75P74 + P1Compositeyes in both S1 and E1yes 7/22
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P76P17+P45Tet QPI+GFP1778pSB1A2 (2079)3857pMB1AmpYesYesYesEX (purified)
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P77RBS + LacI + terminatorsBBa_P03121307Failed (liquid cultures didn't grow)ES purified, XP purifiedyes (purified)
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P78RBS + LacI + terminatorsBBa_P01121310Failed (liquid cultures didn't grow)failed
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P79P90+ P48Composite
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P80P90 + P49Compositeno- wrong
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P83P45+mtrA (w/ Pst1)Composite
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P84heat sensitive Lac- YFPJ069122314pSB1A2 (2079)pMB1AmpYes, in E1.Yes, 7/9
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P85heat sensitive Lac-YFPJ069112314pSB1A2 (2079)pMB1AmpYes, in E1.yes
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P86pPL-PCB from UT Austinn/aP15aAmpYes in S1
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P87pPL-cph1-envZ-pprotetn/aColE1CmNo
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P88light-sensing EnvZBBa_I150102238pSB2K3 (4425)F', P1 lyticKanfailedfailed
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P89RBS + LacI + terminators + pLacBBa_Q011211372pSB1A2 (2079)3451pMB1KanfailedES purified, XP purifiedyes (purified)
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P90CDF in P1 vectorpSB3K3 (2750)p15AKanEX (purified)
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P91P89+P45: lac QPI+complete GFP4327AmpEX (purified)
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P92P84 + P1CompositeQ04510
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P93P85+P1Composite
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P94P40 + mtrBComposite>4000pSB1A2 (2079)Amp
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P95RBS + cph8BBa_S03419Yes
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P96RBS + cph8 + terminatorsBBa_S03417Yes
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P97Strong RBSBBa_B003412pSB1A2 (2079)2.1 kbpMB1AmpYesYes
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P99cell deathBBa_P1010Part from MIT aligned to ref
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P101pSB4C5 (with death gene)pSB4C5/I520021090pSB4C5 (3221)4311pSC101CmYesYesYesPart from MIT aligned to ref
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P102P108+P45 (lac induced GFP) in p15a vector2281pSB3K3 (2750)5031P15AKanYesYesYesYes, along with 108+45#41
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P103P108+P45 (lac induced GFP) in pSC101 vector2281pSB4C5 (3221)5502pSC101CmYesYesYesP103 # 41, 45
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P104P75 + P63Yes 1 colonyyes 7/28
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P105P87 w/o PstI site (mutated Cph)223520224257ColE1CmyesE2P105A, E5P105D
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p106pER21 - plasmid for genetic recombinationn/a : from DadGentamycinYesYes
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p107pKOV - plasmid for genetic recombinationn/a: from George ChurchCmYesYes
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P108P3 + (P39+51) beta (lac QPI)composite1405pSB3K3 (2750)4155p15AKanyesyesyesVerified
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P109RBS+full lacZI7320173093pSB1A2 (2079)5172pMB1Ampyesyesyescorrect from MIT
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P110RBS+lacZalpha+termE0435534pSB4K5 (3419)3953pSC101Kanyesyesyescorrect from MIT
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P111RBS+lacZalpha+termE0435534pSB4C5 (3221)3755pSC101Cmyesyesyescorrect from MIT
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