How much potential does celery decellularization have for relatively cheap and efficient scale up?

Do +90' and -90' aligned cells behave similarly? If so, would the 'flattened' histogram infact appear to be highly aligned at 90'?

Do you think you could seed two cell types into your matrix and have them self sort (for ex endothelial and skeletal cells)

How feasible is co culturing endothelial cells to grow within the celery vasculature, and muscle cells surrounding the vasculature?

Was this at all inspired by the research presented at New Harvest 2018 where a spinach leaf was decellularized to be used as a scaffold?

Was there fusion between scaffold/myocytes, or did fusion simply happen within the scaffold?

Beautiful orientation and differentiation! What cell type do you use for these images?

Was there a “flow” of media along the celery and would that have assisted the alignment?

Does the structure of the substrate effect the potential texture of cells, as a future consumer product?

How do you anticipate it would behave for cellular adhesion, proliferation and migration?

How customizable is the process of culturing meat in celery or plant scaffolding? Would it theoriericallg work with all cellular meat types?

What do the energy inputs look like for maintaining a bioreactor/ are they comparable to those of traditional livestock ag?

Is this an energy-intensive process? Would it be feasible possible to use Solar PV to supply the energy or would that be too costly?

David Smith (University of the West Indies,)

How much work has been done in perfusion mode in terms of cultured meat so far?

How expensive is it to scale the required volume of media and scaffolding as bioreactor size increases?

How expensive is it to scale the required volume of media and scaffolding as bioreactor size increases?

Is there differences in difficulty of measurement/automation-capability between bioreactor design?

Who don’t we see more HFBRs in recombinant protein and cell therapy industry? HFBR seems to be a great solution

Which type of bioreactor is employed most commonly for commercialization currently?

How effective has passage on hollow fiber bioreactors been in collection, and subsequent seeding?

How does the contamination risk in a Hollow Fiber Bioreactor compare to a stirred tank reactor?

Most bioreactors in the industry look to be stirred tanks. Are there any drawbacks of HFB?

Can the polystyrene tunes in a hollow fiber system be used with extra cellular matrix components (collagen, fibronectin, etc)?

How soon will we see large scale cell-based meat production? And will it use hollow fibre bioreactors?

Sophie Kevany, Journalist

What interventions (antimicrobials/processing steps) have you considered for controlling microorganisms in scaled-up reactors?

Zein is super hydrophobic - how did you overcome this to get cell attachment and proliferation?

Do you think openness to eating cell-cultured insect meat would first require experience eating bugs the traditional way? Or could we skip that step?

Aren't you swimming upstream with culturing insect cells, since you're competing with the most sustainable animal?

If insects are usually consumed whole, how can we compete with that culturing their tissues?

Does it make sense from financial and energy concerns to culture insect cells vs. farming whole insects?

Do you eat insects in your daily/weekly diet? Is it hard to convince your lab mates and friends to eat your insect meat?

Do you need less energy to culture insect than vertebrate meat? Is it easier to grow their muscles since they have rudimentary circulatory systems?

David Smith (University of the West Indies,)

How is the taste of skeletal-like muscle cells different from cells from fat or connective tissues?

Would cells harvested from insects be more or less prone to contamination as compared to chordates?

How are insect cells influenced to be in suspension growth and then to adherent growth?

Are you the only person working on culturing insect cells or do you have any collaborators at Tufts or elsewhere?

jasonketola@gmail.com

It sounds like culturing insect cells is way easier than mammalian cells. What are the cellular differences that make it so?

Are the caterpillar and fruit fly cells you're growing meant to be for human consumption?

can insect muscle cells effectively immitate mammalian cells in the final consumer product?

Do insect cells grow better at low oxygen condition since their natural oxygen transport mechanisms are so different?

Given insects are already much cheaper, scalable, and sustainable over other animal meats, what is the value proposition of lab grown insect meat?

What are the key features of insect cells that allow them to grow on (low amounts of) serum-free media that other type of cells typically require?

What is the extent that the insect cells are affected by media, could it change cell properties?