BioTechBuilder - MA CTE Chapter 74 Biotech Framework (2014) Alignment

	A	B	C	D	E	F	G	H	I	J
1	BioTechBuilder Curriculum Alignment to MA Chapter 74 Biotech Framework					(June 2014 version)
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3	The detailed alignment of the BTB curriculum to Strand 2 is shown below. Students completing Module 1 of BioTechBuilder curriculum will also learn to identify and utilize Safety Data Sheet (SDS) information. They will also learn to explain the handling of hazardous materials and be able to respond to an emergency in a lab (Strand 1 frameworks). Oral and written communication skills are emphasized throughout the curriculum as students work in pairs or in small groups, documenting their results and discussions, and receiving feedback from peers and instructors. The curriculum also includes opportunities to present an experimental outcome or a research project (Strand 4 frameworks).
4	LAB SKILLS			FOUNDATIONS		APPLICATIONS		STRAND 2 TECHNICAL KNOWLEDGE AND SKILLS
5	Building a Solid Foundation	Making Solutions	Growing Cells	Analyzing DNA	Analyzing Proteins	Biomanufacturing	Synthetic Biology	2.A SAFETY IN THE BIOTECHNOLOGY LABORATORY
6								2.A.01	Complete and obtain a 10-hr OSHA certification
7									2.A.01.01	Complete the requirements of OSHA 10 hour certification course and receive a course completion card
8								2.A.02	Complete and obtain a CPR and FIrst Aid Training card/credential.
9									2.A.02.01	Complete American Red Cross or American Heart Association Heart saver First Aid and CPR AED certification and receive a course completion card
10								2.A.03	Follow safety and emergency procedures according to OSHA standards
11									2.A.03.01	Practice work habits that provide personal safety, safety for others, and protect the safety and security of the external environment
12									2.A.03.02	Select and use appropriate personal protective equipment at all times
13									2.A.03.03	Maintain a sanitary and clutter-free work environment
14									2.A.03.04	Monitor, use, store, and dispose of materials according to established procedures
15								2.B BASIC BIOTECHNOLOGY KNOWLEDGE AND SKILLS
16								2.B.01	Demonstrate knowledge of biotechnology industry fundamentals
17									2.B.01.01	Describe the major application areas of biotechnology and their products
18									2.B.01.02	Describe the life cycle of products (e.g., research and development to production).
19									2.B.01.03	Describe the organizational structures of biotechnology companies
20									2.B.01.04	Summarize the historical development of biotechnology
21									2.B.01.05	Describe the social, legal, and ethical issues that affect the application of biotechnology
22									2.B.01.06	Use and interpret information resources relevant to biotechnology (e.g., journals, databases, website, etc.)
23									2.B.01.07	Explain the different career paths in biotechnology and the jobs associated with them
24								2.B.02	Demonstrate knowledge of regulatory affairs.
25									2.B.02.01	Explain good documentation practices (e.g., signatures, dating, use of indelible ink, witnessing requirements)
26									2.B.02.02	Explain the history of pharmaceutical regulations and the Food and Drug Administration (FDA)
27									2.B.02.03	Explain the organization of the FDA (e.g., the roles of Center for Drug Evaluation and Research (CDER) and Center for Biologics Evaluation and Research (CBER))
28									2.B.02.04	Explain the life cycle of medical products (e.g., discovery through clinical trials, New Drug Application (NDAs), Investigational New Drug (INDs)
29									2.B.02.05	Explain current Good Manufacturing Practice (cGMP) and Good Laboratory Practice (GLP) regulations
30									2.B.02.06	Explain the regulatory agencies at the local, state and federal levels
31									2.B.02.07	Document assay procedures and results
32									2.B.02.08	Evaluate results of assays (e.g., determine amount of analyte in quantitative assay).
33									2.B.02.09	Prepare results in written technical reports (e.g., designated report format is used, all resources are referenced, graphs and tables are clearly labeled and explained, data is accurately analyzed) and present orally
34									2.B.02.10	Explain the roles of the IBC and IACUC
35									2.B.02.11	Explain United States Department of Agriculture (USDA) an Public Health Service (PHS) Policy on the humane care and use of laboratory animals and the roles of the American Association for Laboratory Animal Science (AALAS) and Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC).
36								2.B.03	Demonstrate basic lab management skills
37									2.B.03.01	Inventory supplies according to established procedures
38									2.B.03.02	Obtain required materials
39									2.B.03.03	Schedule work functions in an organized manner
40									2.B.03.04	Clean and sterilize glassware and counters according to pre established procedures
41									2.B.03.05	Ensure that equipment is cleaned and maintained according to established preventative maintenance procedures
42									2.B.03.06	Document lab support functions using logbooks, computer systems, forms, and other methods, according to established procedures (e.g., autoclave, pH meter, incubators and freezers).
43								2.C BIOMANUFACTURING FUNDAMENTALS
44								2.C.01	Demonstrate and apply manufacturing process management techniques
45									2.C.01.01	Identify, create and use standard operating procedures (SOPs).
46									2.C.01.02	Explain how process control data is used to monitor processes
47									2.C.01.03	Test product to verify that it meets specifications and regulations
48									2.C.01.04	Explain biomanufacturing lean techniques as they pertain to improved efficiency, reduction of waste and increased productivity
49									2.C.01.05	Identify and apply the concepts of total quality management appropriate to the field
50									2.C.01.06	Develop a comprehensive product development plan for a biotechnology product
51								2.D SOLUTION PREPARATION
52								2.D.01	Perform basic calculations.
53									2.D.01.01	Perform calculations relating to measurements.
54									2.D.01.02	Perform calculations relating to reagent and media formulation and dilution
55									2.D.01.03	Perform calculations relating to data acquisition and analysis
56									2.D.01.04	Perform calculations relating to products, processing and quality control monitoring
57									2.D.01.05	Perform calculations relating to growing cells and analyzing their growth rate
58									2.D.01.06	Determine concentrations of solutions
59									2.D.01.07	Graph and interpret data using electronic spreadsheet programs
60									2.D.01.8	Perform calculations relating to dosage levels for animal studies (e.g., mg/kg, ml/kg).
61								2.D.02	Prepare solutions.
62									2.D.02.01	Prepare percent, molar and molal solutions
63									2.D.02.02	Perform dilutions and calculate resulting concentrations
64									2.D.02.03	Perform serial dilutions
65									2.D.02.04	Prepare agar plates and broths using appropriate techniques (filtration or autoclave).
66									2.D.02.05	Label and store reagents, solutions, and media according to established procedures
67								2.E INSTRUMENTATION AND LAB ASSAYS
68								2.E.01	Metrology: Use measurement instrumentation.
69									2.E.01.01	Calibrate and maintain measuring instruments (e.g., balances, pH meters, thermometers, pipettes, spectrophotometers)
70									2.E.01.02	Make weight measurements with acceptable accuracy and precision
71									2.E.01.03	Make volume measurements with acceptable accuracy and precision
72									2.E.01.04	Make pH measurements with acceptable accuracy and precision
73									2.E.01.05	Make temperature measurements with acceptable accuracy and precision
74									2.E.01.06	Make spectrophotometric measurements with acceptable accuracy and precision
75								2.E.02	Use microscopes
76									2.E.02.01	Identify microscope parts
77									2.E.02.02	Demonstrate use and care of microscopes.
78									2.E.02.03	Differentiate between the different types of microscopes and describe their uses (compound, inverted, scanning electron, fluorescent, and stereo).
79									2.E.02.04	Prepare a wet mount slide
80									2.E.02.05	Prepare a slide with a cross section
81									2.E.02.06	Demonstrate cleaning of the microscope and lenses.
82									2.E.02.07	Demonstrate basic cell staining techniques to observe cell morphology
83									2.E.02.08	Count cells using a hemocytometer
84									2.E.02.09	Identify and describe functions of cellular organelles
85								2.E.03	Perform standard lab assays/techniques
86									2.E.03.01	Isolate DNA
87									2.E.03.02	Determine DNA concentration and purity
88									2.E.03.03	Perform enzyme-linked immunosorbent assay (ELISA).
89									2.E.03.04	Perform western blotting
90									2.E.03.05	Perform DNA fingerprinting
91									2.E.03.06	Perform Polymerase Chain Reaction (PCR).
92									2.E.03.07	Perform gram staining
93									2.E.03.08	Determine protein concentration
94									2.E.03.09	Determine enzyme activity
95								2.E.04	Perform separation techniques
96									2.E.04.01	Operate centrifuges
97									2.E.04.02	Use filtration devices and systems
98									2.E.04.03	Use electrophoresis systems (e.g., SDS PAGE and agarose gel electrophoresis).
99									2.E.04.04	Use chromatography (e.g., size exclusion, affinity, HIC, ion exchange).
100									2.E.04.05	Compare and contrast various chromatography methods. (including size exclusion, HPLC, reverse phase, affinity, HIC, ion exchange, etc.).