365 Days Of Science
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DateTopicInstagram linkblog link
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8/21/19standard curveshttp://bit.ly/2Nu9dAS
http://bit.ly/2NoXzHA
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8/20/19serial dilutionshttp://bit.ly/2P4GcOR
http://bit.ly/2ZpLPLC
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8/19/19diluting strong acidshttp://bit.ly/2Z3q1pn
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8/18/19
isolectric point (pI); weak acids & bases; protein charge
http://bit.ly/30qzHH6
http://bit.ly/2Z3rAnC
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8/17/19
acids, bases, pH, and pKa - cookie jar analogy
http://bit.ly/2KPzBCn
http://bit.ly/2Zj3mFe
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8/16/19agarose gel electrophoresis
http://bit.ly/2MmWsJ0
http://bit.ly/2Z6vmeR
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8/15/19PCR primer designhttp://bit.ly/2KC9DDJ
http://bit.ly/30ejiWo
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8/14/19PCR (polymerase chain reaction)
http://bit.ly/31KkGAt
http://bit.ly/31GrYoJ
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8/13/19vector plasmids
http://bit.ly/2H7dnLb
http://bit.ly/2YI8PWq
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8/12/19
protein expression slides - bakery analogy
http://bit.ly/300UPUk
link to slides
http://bit.ly/2Tpsdlh
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8/11/19PCR purification, spin columns
http://bit.ly/2ySEftU
http://bit.ly/2yO5BBt
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8/10/19minipreps (alkaline lysine)
http://bit.ly/2MNGJ50
http://bit.ly/31yTlkK
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8/9/19SDS-PAGE
http://bit.ly/2GZc3tG
http://bit.ly/2OYbc38
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8/8/19SDS PAGE (animated); TEV purificationhttp://bit.ly/2TmpSr9
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8/7/19
baculovirus expression vector systems video, insect cell expression
http://bit.ly/2YQtPFJ
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8/6/19clear vs colorlesshttp://bit.ly/31lrvbl
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8/5/19
reporting error, measurement uncertainty, standard deviation
http://bit.ly/33hkCd8
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8/4/19
standard deviation and standard error of the mean
http://bit.ly/2yE7PTS
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8/3/19significant figures (sig figs)http://bit.ly/2T1zN5r
http://bit.ly/2LYEoEz
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8/2/19
accuracy vs. precision, types and sources of error
http://bit.ly/2Ke3gpX
http://bit.ly/2YFJYle
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8/1/19stock & working solutionshttp://bit.ly/2Mxl40P
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7/31/19relative concentrations, % w/vhttp://bit.ly/2OwH4LJ
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7/30/19
dimensional analysis, SI units, unit conversion
http://bit.ly/2LR4CZA
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7/29/19stock solutions, M1V1
http://bit.ly/2yogj1i
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7/27/19
insect cell protein expression - baculovirus expression vector systems (BEVS)
http://bit.ly/2SLCt6N
http://bit.ly/2K8LmDF
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7/26/19
RNA interference (RNAi); microRNA (miRNA)
http://bit.ly/2MgXTHL
http://bit.ly/2JViyjc
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7/25/19
hydrogen deuterium exchange mass spectrometry (HDX-MS)
http://bit.ly/2JUgdVP
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7/24/19WAVE bioreactor; insect cell expressionhttp://bit.ly/2OhWL9t
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7/23/19desiccantshttp://bit.ly/2YfkyXf
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7/22/19humidity and hair frizzhttp://bit.ly/2K03iQM
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7/21/19humidityhttp://bit.ly/2Y58uYH
http://bit.ly/2JYPZQR
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7/20/19analytical size exclusion chromatographyhttp://bit.ly/2xWH7VZ
http://bit.ly/2GmWknW
c
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7/19/19states of matter, liquid nitrogenhttp://bit.ly/30JJBni
http://thebumblingbiochemist.com/365-days-of-science/liquid-nitrogen-states-of-matter/
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7/18/19alkali metals, lyeshttp://bit.ly/2xWfPzk
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7/17/19salt solubility; potassium in SDS-PAGEhttp://bit.ly/2XW4IRh
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7/16/19silver staininghttp://bit.ly/2JGHuto
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7/15/19alkaline batterieshttp://bit.ly/2JHuAeE
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7/14/19
batteries, galvonic (voltaic) cells vs electrolytic cells
http://bit.ly/2xJpXLE
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7/13/19diabetes, insulin, glucometershttp://bit.ly/2Gcy1t3
http://bit.ly/2NU2S3y
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7/12/19carbohydrateshttp://bit.ly/2JwIuRV
http://thebumblingbiochemist.com/365-days-of-science/carbohydrates/
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7/11/19protease inhibitors, PMSF, serine proteaseshttp://bit.ly/2SgSLEM
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7/10/19RNaseA, DEPChttp://bit.ly/2XHJKWa
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7/9/19
metals, metalloproteins, coordinate covalent bonds
http://bit.ly/2XDSLjd
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7/8/19
redox vs acid/base; electronegativity; oxidation numbers
http://bit.ly/2XBhNUQ
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7/7/19chemistry of glowstickshttp://bit.ly/2NEid8w
http://bit.ly/2JjmKZF
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7/6/19
hydrogen peroxide, disproportionation reactions, decomposition, electronegativity
http://bit.ly/2JFRLGr
http://bit.ly/2NCcrEs
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7/5/19lipidshttp://bit.ly/2Yx1Lb0
http://bit.ly/2XvaxVT
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7/4/19free energy couch analogy; fireworkshttp://bit.ly/2XrKuUi
http://bit.ly/2JaqYTw
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7/3/19electrolytic cells, gel electrophoresishttp://bit.ly/2XMLMbG
http://thebumblingbiochemist.com/365-days-of-science/electrolytic-cells-gel-electrophoresis/
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7/2/19proteases, nucleaseshttp://bit.ly/2XLvArd
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7/1/19perms, rubber bands, vulcanizationhttp://bit.ly/2FJUXPU
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6/30/19
acrylamide polymerization; polyacrylamide; APS; TEMED; free radicals
http://bit.ly/2FFI9dd
http://bit.ly/2NkBoUt
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6/29/19redox, reducing agents, disulfide bonds
http://bit.ly/2Yiya50
http://bit.ly/2FTnVx5
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6/28/19
stripping & regenerating IMAC resin, Ni-NTA, His tag
http://bit.ly/2XGQiZ6
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6/27/19fusion proteins, GST, SUMOhttp://bit.ly/2LoRzOg
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6/26/19precipitationhttp://bit.ly/2Xws1VP
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6/25/19affinity tagshttp://bit.ly/2XBu9eI
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6/24/19
His-tag, IMAC, Ni-NTA, EDTA, chelation, affinity chromatography
http://bit.ly/2Fs8taY
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6/23/19monoclonal & polyclonal antibodies
http://bit.ly/2ZIatna
http://bit.ly/2Md7nEb
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6/22/19antbodieshttp://bit.ly/31PvrlO
http://thebumblingbiochemist.com/365-days-of-science/antibodies/
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6/21/19translation
http://bit.ly/31IwofL
http://thebumblingbiochemist.com/365-days-of-science/translation/
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6/20/19
spectroscopy purity, RNA, DNA, protein, 260/230, 260/280
http://bit.ly/2KtUbL3
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6/19/19NanoDrop, spectroscopy
http://bit.ly/2KZ8vKQ
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6/18/19Beer-Lambert law, spectroscopyhttp://bit.ly/2RmRHyo
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6/17/19rust, metals, oxidationhttp://bit.ly/31MPb9w
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6/16/19
chain terminators - reverse transcriptase inhibitors, dye terminators, HIV, AZT
http://bit.ly/2Xb5LAL
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6/15/19senior citizen science engagementhttp://bit.ly/2KvFPJX
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6/14/19nucleic acids
http://bit.ly/2FqasfN
http://thebumblingbiochemist.com/365-days-of-science/nucleic-acids/
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6/13/19ion exchange chromatography
http://bit.ly/2WKgxyg
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6/12/19
buffers - pH, pKa, Henderson-Hasselbach equation, Tris, TBS
http://bit.ly/2X4Yhz7
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6/11/19
detergents - and soaps & phospholipids; tween vs SDS
http://bit.ly/2XKuhWs
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6/10/19western blot - blocking (BSA, milk)
http://bit.ly/2wIULM2
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6/9/19western blot - wet electrotransfer
http://bit.ly/2XzNRV3
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6/8/19western blot overview
http://bit.ly/2Iy5b6r
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6/7/19levels of protein structure
http://bit.ly/2KBFRiS
http://thebumblingbiochemist.com/365-days-of-science/layers-of-protein-structure/
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6/6/19desalting columns, buffer exchange, dialysishttp://bit.ly/2KxDEVF
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6/5/19
beta-lactam antibiotics (cephalosporins, penicillins)
http://bit.ly/31beK3X
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6/4/19locked nucleic acidshttp://bit.ly/2KuxZiX
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6/3/19solid-state oligonucleotide synthesishttp://bit.ly/2We8e8W
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6/2/19blue-white screeninghttp://bit.ly/2Xm6prR
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6/1/19
RNA extraction (TRIzol/ acid guanidinium thiocyanate–phenol–chloroform extraction)
http://bit.ly/2Xj4Zyc
http://thebumblingbiochemist.com/365-days-of-science/rna-extraction/
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5/31/19
molecules - elements, atoms, covalent bonds, atomic radius
http://bit.ly/2wvkWWv
http://thebumblingbiochemist.com/365-days-of-science/molecules/
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5/30/19
nusinersen; SMA; RNA splicing regulation; antisense oligonucleotides
http://bit.ly/2K9G4tk
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5/29/19acids, bases, & pH - cookie jar analogyhttp://bit.ly/2KcZTjk
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5/28/19ultrasonic DNA shearinghttp://bit.ly/2JIJ6W7
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5/27/19
workflow for expressing & purifying a recombinant protein - with emphasis on cloning and expression
http://bit.ly/2JY6cWi
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5/26/19
workflow for expressing & purifying a recombinant protein
http://bit.ly/2VMELTe
http://bit.ly/2JPROAz
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5/25/19
FPLC (fast protein liquid chromatography) - AKTA
http://bit.ly/30LklxG
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5/24/19intro to me - 1st post for the IUBMBhttp://bit.ly/2WoDBSb
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5/23/19protein chromatography
http://bit.ly/2VKBJz0
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5/22/19binding affinity, Kdhttp://bit.ly/2JyBbuj
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5/21/19
size exclusion chromatography & SDS-PAGE comparison
http://bit.ly/2EphbGt
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5/20/19FLex recombinationhttp://bit.ly/2Hx23J7
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5/19/19Cre-loxP recombination
http://bit.ly/2HtdAcm
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5/18/19
CRISPR/Cas9 as a bacterial immune system vs. our immune systems
http://bit.ly/2JQ1Baq
http://thebumblingbiochemist.com/365-days-of-science/the-real-crispr-cas-how-it-compares-to-our-immune-systems/
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5/17/19forward vs reverse geneticshttp://bit.ly/2W5hGzt
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5/16/19
genetic engineering - cell biology vs. biochemistry
http://bit.ly/2EfCycW
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5/15/19PAM sequences - Crisprhttp://bit.ly/2HlCKJG
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5/14/19CRISPR/Cas9 vs. RNAi
http://bit.ly/30mHjuE
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