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InstagramBlogYouTube
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9/23/21
Thesis Thoughts: style, acknowledgments section, & how it feels post-submission (Hint: pretty good!)
https://bit.ly/3ADfMaghttps://bit.ly/thesisthoughts
https://youtu.be/VzphVydld0M
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9/22/21
Why I dedicated my thesis to Grammy, plus a call for senior citizen science outreach
https://bit.ly/3lMs0ashttps://bit.ly/thesisdedication
https://youtu.be/yxWmfP2uR5A
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9/21/21thesis submittedhttps://bit.ly/3tZ6932https://bit.ly/thesissubmitted
https://youtu.be/c49p2vytbYs
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9/20/21
Mendeley and other reference management tools + tips for finding & managing the correct references!
https://bit.ly/3Ar8jejhttps://bit.ly/referencesreference
https://youtu.be/VIds4W2nut8
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9/19/21intro to metabolism talkhttps://bit.ly/3zmyQIjhttp://bit.ly/letstalkmetabolism
https://youtu.be/yb9e2piqhTE
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9/18/21thermodynamics, entropy, enthalpyhttps://bit.ly/3ChbllUhttps://bit.ly/thermodynamicstalk
https://youtu.be/oX0KAJWnuog
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9/17/21How ATP stores energy - with short moviehttps://bit.ly/3khnu47https://bit.ly/ATPasenergymoney
https://youtu.be/RhpVIwWl1KM
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9/16/21the bumbling biochemist blog tourhttps://bit.ly/3EjF8MFhttps://bit.ly/bbblogtour2
https://youtu.be/gDT30nFOipo
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9/15/21AKTA; Fast Protein Liquid Chromatography (FPLC) - extended videohttps://bit.ly/39bMNhKhttps://bit.ly/aktainaction
https://youtu.be/KywmjA3uucA
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9/14/21biochemistry lab equipment, extended videohttps://bit.ly/3Ab7NB6http://bit.ly/biochemistrylabequipment
https://youtu.be/dU8ivYV1doI
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9/13/21
Ago again?! RNA interference (RNAi), microRNA, and Argonaute (Ago) – an intro
https://bit.ly/3htgC1Lhttps://bit.ly/RNAi_intro_vid
https://youtu.be/fn0WNxO3UTk
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9/12/21lab people - who's who and what they do (extended version)https://bit.ly/3z2F8N3http://bit.ly/labspeople
https://youtu.be/6JHRyO1BEf8
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9/11/219/11 thoughtshttps://bit.ly/3C4YX8k
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9/10/21lab notes and organizationhttps://bit.ly/3hkeGZahttps://bit.ly/labnotesorganization
https://youtu.be/JGzSUz88S-g
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9/9/21
baculovirus expression vector systems (BEVS) (insect cell protein expression)
https://bit.ly/3jXNSQdhttps://bit.ly/baculovirusexpression
https://youtu.be/94SvIiSCNLY
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9/8/21make experimental figures along the way for your notes and needshttps://bit.ly/3BUssdahttps://bit.ly/makeexperimentfigures
https://youtu.be/AccUD9os7wI
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9/7/21site-directed mutagenesis & protein expression construct termshttps://bit.ly/mutagenesisconstructs
https://youtu.be/vm6p5Nq2sTg
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9/6/21
Bumbling biochemist behind the scenes of bumbling & Googling her way to making an Ago intro - adobe premiere & aftereffects attempts
https://bit.ly/3BLtOXphttps://bit.ly/bumblymoviemaking
https://youtu.be/i6IYkMEO59Y
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9/5/21
1 month to my PhD thesis defense! Here's why that event means so much for me…
https://bit.ly/38F5om5https://bit.ly/1monthtodefense
https://youtu.be/sAado2XEyA0
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9/4/21c1v1=c2v2; mixing solutions, molarity, concentrationshttps://bit.ly/3zOnb65https://bit.ly/c1v1vid
https://youtu.be/rYEJTiPL3UU
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9/3/21agarose gel electrophoresis and comparison to SDS-PAGEhttps://bit.ly/3zJ8elXhttps://bit.ly/agarosegelcompare
https://youtu.be/vbuxf3rcMxg
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9/2/21thesis-zoning outhttps://bit.ly/3BAkuphhttps://bit.ly/thesiszoningout
https://youtu.be/sYY6Cgn3hYo
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9/1/21wikipedia editinghttps://bit.ly/38ysvhWhttp://bit.ly/wikieditingintro
https://youtu.be/6Z2YnYSD3k4
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8/31/21
Thesis-izing: a quick video on writing a thesis, what that means, & what I've found useful
https://bit.ly/2Y8hCRXhttps://bit.ly/thesisizing
https://youtu.be/2fb4k33F3Bk
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8/30/21hair frizz, bond types, intermolecular forces, hydrogen bondshttps://bit.ly/3zuSxyFhttp://bit.ly/frizzandmolecularattractions
https://youtu.be/CBXR7_oMT8o
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8/29/21
Using the PDB to investigate x-ray crystallography structures: resolution, R-factor, outliers & more
https://bit.ly/pdbstructures
https://youtu.be/IZtHsUFbyes
https://youtu.be/IZtHsUFbyes
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8/28/21RNAi - an intro work in progress in need of much more workhttps://bit.ly/3mH7XMzhttps://bit.ly/RNAiintrodraft
https://youtu.be/iDSfPjkEgCI
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8/27/21some study suggestions for schoolhttps://bit.ly/3mFBKFyhttps://bit.ly/studysuggestions
https://youtu.be/UR0uF_aKAZg
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8/26/21
optimizing and troubleshooting protein expression, inclusion bodies, second video
https://bit.ly/3gAxYJDhttp://bit.ly/wherestheprotein
https://youtu.be/jTQxHK3o3lE
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8/25/21mRNA splicing and alternative splicinghttps://bit.ly/3jdrYbshttps://bit.ly/mRNAsplicing
https://youtu.be/0P6OAljhv0c
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8/24/21ion exchange chromatography, isolectric point (pI)https://bit.ly/38aEsdAhttp://bit.ly/ionexchangechromatography
https://youtu.be/XCiPRYUBo34
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8/23/21
UniProt, ProtParam, and other useful free online protein database tools - the basics
https://bit.ly/uniprotprotparam
https://youtu.be/6oBsTykEeGI
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8/22/21
How proteins take shape - amino acids, peptide bonds, & layers of protein structure
https://bit.ly/3DasgHWhttps://bit.ly/proteinstructure
https://youtu.be/-9F7B_A7Lqk
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8/21/21protein structure prediction software (AlphaFold & Rosetta)https://bit.ly/3gp4GNQhttps://bit.ly/proteinpredictionponderings
https://youtu.be/uKSgTdLxQQw
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8/20/21Radiometric kinase assays with scintillation countinghttps://bit.ly/2XIQkSahttps://bit.ly/kinaseassays
https://youtu.be/qG2iGtbAhw0
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8/19/21ChimeraX's webcam feature https://bit.ly/chimeraxwebcam
https://youtu.be/E42aImuftCw
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8/18/21Argonaute, RNAi, and how structural biology helps us spy!https://bit.ly/agostructurestuff
https://youtu.be/IvDfTU6rsK4
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8/17/21intro to PyMol movies & alignmenthttps://bit.ly/pymolmovies
https://youtu.be/BQVpI0m10Os
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8/16/21intro to PyMolhttps://bit.ly/3jX7S4thttps://bit.ly/pymolintro
https://youtu.be/GpxCGQAhgi8
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8/15/21mRNA vaccine biochemistry videohttp://bit.ly/mRNAvaccinebiochem
https://youtu.be/dXmzKVBcuD0
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8/14/21
Kinases & phosphatases make a great team! A random animation to illustrate how I use Illustrator!
https://bit.ly/3jUbEeMhttps://bit.ly/kinaseanimationexample
https://youtu.be/PPPpbvAhZmc
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8/13/21
Radioactivity from a biochemistry perspective: nuclear isotopes, nuclear decay and radiolabeling
https://bit.ly/3yMIephhttps://bit.ly/radioactivitybiochem
https://youtu.be/RopVjOniwxM
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8/12/21SDS-PAGE theory & practice: into the buffer and behind the scenes!https://bit.ly/sdspagepractical
https://youtu.be/FgXlPAKVSGY
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8/11/21Adobe Illustrator - key features & tips for getting by https://bit.ly/adobeillustratortips
https://youtu.be/r0F1-a-RTPE
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8/10/21
Making Geeky Graphics - vector graphics programs (esp. Adobe Illustrator) explained by a non-expert
https://bit.ly/geekygraphics
https://youtu.be/Yir8oUxL8aw
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8/9/21Tsuneko Okazaki & Okazaki fragments - video addedhttps://bit.ly/2X6M5iNhttps://bit.ly/tsunekookazaki
https://youtu.be/BWF3A1DOV8U
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8/8/21Tsuneko Okazaki & Okazaki fragmentshttps://bit.ly/37veuBkhttps://bit.ly/tsunekookazaki
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8/7/21western blot "lecture" (more formal look than a couple days ago)http://bit.ly/westernblotwalkthrough
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8/6/21virtual scientific conferenceshttps://bit.ly/37qjbMMhttps://bit.ly/virtualscientificconferences
https://youtu.be/OE4OSVV5Bn8
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8/5/21western blot - behind the scenes (practical version)https://bit.ly/2VjYXlrhttps://bit.ly/westernblotpractical
https://youtu.be/yQgUgR38KDg
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8/4/21
Slot-blot-a-lot-a-lotting...filter binding assays & general thoughts on biochemistry experimenting
https://bit.ly/slotblotalot
https://youtu.be/fcfcsIxVB-E
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8/3/21How rapid coronavirus tests work (ID NOW, antigen, etc.) - videohttps://bit.ly/3zYyR62https://bit.ly/rapidcoronavirustests
https://youtu.be/KHKJJVbWG9Q
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8/2/21
Types of coronavirus tests - the Cliff's notes version - molecular, antigen, antibody, etc.
https://bit.ly/3rMQO4yhttps://bit.ly/coronavirustesttypes
https://youtu.be/pIzSUxFTpc8
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8/1/21
microRNA or siRNA + Argonaute (Ago) = the ultimate RISC! RNA interference (RNAi) & Ago awesomeness
https://bit.ly/3ylTCIghttps://bit.ly/awesomeAgo
https://youtu.be/xrEDCPI5NAc
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7/31/21burnout in grad school and competitionhttps://bit.ly/3ln9maBhttps://bit.ly/bbburnout
https://youtu.be/RWG9tWtf73o
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7/30/21How coronavirus tests work (the RT-PCR ones) - videohttps://bit.ly/3zKAUumhttps://bit.ly/coronavirustesttalk
https://youtu.be/mLa6Tcd27jQ
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7/29/21
Neutralizing antibody biochemistry - what they are, why they're awesome, and how they're tested for
https://bit.ly/neutralizingantibodybiochemistry
https://youtu.be/Z5vAP1Z1oSM
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7/28/21coronavirus Spike protein biochemistry part 2https://bit.ly/spikebiochemistryp2
https://youtu.be/22M2Wh6rQmA
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7/27/21coronavirus Spike protein biochemistry part 1https://bit.ly/3rA6vvWhttps://bit.ly/spikebiochemistry
https://youtu.be/BJ7eN-94j94
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7/26/21bumbly cryo-EM introhttps://bit.ly/cryoEMbumblyintro
https://youtu.be/qcS6oZlEtH0
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7/25/21Proline-based stabilization of coronavirus Spike proteinshttps://bit.ly/prolineperforms
https://youtu.be/chScI8lPpAE
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7/24/21kinase mechanismhttps://bit.ly/3i0M8EQhttps://bit.ly/kinasesandphosphorylation
https://youtu.be/lBqPOAeI2d0
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7/23/21kinases and phosphorylationhttps://bit.ly/3x12tOfhttps://bit.ly/kinasesandphosphorylation
https://youtu.be/OjuVoDBlUes
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7/22/21
open access, journal articles, preprints, reading critically, browser extensions
https://bit.ly/3ruh9nPhttp://bit.ly/openaccesspreprints
https://youtu.be/vjr3ANdiYHo
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7/21/21flash freeze dance, vitrification, cryoprotectantshttps://bit.ly/2V1pX8Lhttps://bit.ly/cryoprotectantsandvitrification
https://youtu.be/vjr3ANdiYHo
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7/20/21ultrasonication, DNA shearing, lysishttps://bit.ly/3rmenRshttps://bit.ly/ultrasonicshearing
https://youtu.be/cmN9COt3ikM
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7/19/21
Journal club group meetings (& more "random" stuff about papers & science twitter)
https://bit.ly/3rjoc2Lhttps://bit.ly/journalclubmeetings
https://youtu.be/cmN9COt3ikM
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7/18/21nothing newhttps://bit.ly/3zfOuFV
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7/17/21
Intro to PDB (Protein Data Bank) crystal structure entries & crystal contents & terms
https://bit.ly/3ine1G5https://bit.ly/pdbstructures
https://youtu.be/Re2gwi-_OEw
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7/16/21Alexandra Newton president of IUBMBhttps://bit.ly/3kFpMe7https://bit.ly/congratspresnewton
https://youtu.be/Re2gwi-_OEw
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7/15/21crystal harvesting and cryoloop preparation and recyclinghttps://bit.ly/3enlXX0https://bit.ly/crystalharvest
https://youtu.be/E8RH3OFiM4s
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7/14/21Behind the scenes of crystal screenshttps://bit.ly/36CmtMghttps://bit.ly/crystallizingproteins
https://youtu.be/E8RH3OFiM4s
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7/13/21
Protein crystallization - Give your protein a nice cocktail!: precipitants, crystallization diagrams, screening and optimization
https://bit.ly/2Vz3xf3https://bit.ly/crystallizingproteins
https://youtu.be/E8RH3OFiM4s
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7/12/21
Intro to protein crystallization (the actually getting crystals stuff and why it's so hard)
https://bit.ly/3AZjg7Ihttps://bit.ly/gettingcrystals
https://youtu.be/E8RH3OFiM4s
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7/11/21
Using the PDB to investigate x-ray crystallography structures: resolution, R-factor, outliers & more
https://bit.ly/2TTa442https://bit.ly/pdbstructures
https://youtu.be/IZtHsUFbyes
https://youtu.be/IZtHsUFbyes
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7/10/21jargon watch - assay, residue, aliquothttps://bit.ly/36pVTFZhttps://bit.ly/biochemjargon
https://youtu.be/ZffY6f6pxvE
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7/9/21PKU (phenylketonuria)https://bit.ly/3AOdOnJhttp://bit.ly/pkustoryandscience
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7/8/21protein concentrating; centrifugal ultrafiltrationhttps://bit.ly/3hthO5qhttps://bit.ly/spinconcentrators
https://youtu.be/jIcDEZN2BT0
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7/7/21
IMAC (immobilized metal affinity chromatography) for His-tag purification
https://bit.ly/3hQyN0Shttps://bit.ly/histagIMAC
https://youtu.be/oX0KAJWnuog
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7/6/21thermodynamics, entropy, enthalpyhttps://bit.ly/3hkXZNEhttps://bit.ly/thermodynamicstalk
https://youtu.be/oX0KAJWnuog
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7/5/21family stuffhttps://bit.ly/3dTtDzV
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7/4/21fireworks, thermodynamicshttps://bit.ly/3jEZ2K4https://bit.ly/fireworksscience
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7/3/21protein translationhttps://bit.ly/proteintranslationwedding
https://youtu.be/L7AKk9X7GDY
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7/2/21getting through rough days in sciencehttps://bit.ly/2V55fVphttps://bit.ly/sciencestrugglesandstrength
https://youtu.be/E-VJWiq54Ag
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7/1/21get vaccinated for grandmas
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6/30/21AKTA; Fast Protein Liquid Chromatography (FPLC)https://bit.ly/aktainaction
https://youtu.be/E-VJWiq54Ag
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6/29/21
size exclusion chromatography (SEC), aka gel filtration, analytical vs. preparative, types of resin
https://bit.ly/3x5Ii2h
https://bit.ly/sizeexclusionchromatographytalk
https://youtu.be/4-3fYDZ9mUY
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6/28/21strep tag purification - streptavidin, avidin, biotinhttps://bit.ly/2URb74Fhttps://bit.ly/streptagaffinity
https://youtu.be/x9RraY_W9kY
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6/27/21bacterial growth media; LB, TB, SOB, SOC, etc.https://bit.ly/3gVAg6Ghttps://bit.ly/bacterialgrowthmedia
https://youtu.be/Yjb1jvplywA
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6/26/21Novavax COVID-19 vaccine biochemistryhttps://bit.ly/35XUoPjhttps://bit.ly/novavaxvaccine
https://youtu.be/xmJX5t2g3KE
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6/25/21
baculovirus expression vector systems (BEVS) (insect cell protein expression)
https://bit.ly/2U27DvJhttps://bit.ly/baculovirusexpression
https://youtu.be/94SvIiSCNLY
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6/24/21blue-white screeninghttps://bit.ly/3qqdM0whttps://bit.ly/bluewhitescreening
https://youtu.be/Zyt42aIu7Bc
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6/23/21antibiotic selectionhttps://bit.ly/3xQMFORhttps://bit.ly/antibioticselectionscience
https://youtu.be/RYgifzHe61g
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6/22/21colony PCR chathttps://bit.ly/3x2hkcdhttp://bit.ly/colonyPCRchat
https://youtu.be/Ve4I9ELBleA
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6/21/21lab people - who's who and what they dohttps://bit.ly/35F4NiPhttp://bit.ly/labspeople
https://youtu.be/94SvIiSCNLY
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6/20/21what "expression" means and ways it is measured, videohttps://bit.ly/2SHBCZA
http://bit.ly/expressionmeaningandmeasuring
https://youtu.be/5NqPYiZ8qhw
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6/19/21Alice Ball, chaulmoogra oil, leprosy, videohttps://bit.ly/3iTzMPZhttp://bit.ly/aliceballmethod
https://youtu.be/5NqPYiZ8qhw
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6/18/21
The importance of (and current lack of) diversity in medical research & how Dr. Namandje Bumpus is getting people to take notice
https://bit.ly/3q5oYQghttps://bit.ly/diversitybumpus
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6/17/21
optimizing and troubleshooting protein expression, inclusion bodies, video
https://bit.ly/35xcSpKhttp://bit.ly/wherestheprotein
https://youtu.be/QyVmWYDLzUo