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eDNA Materials List
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Organized by Purpose of Supplies
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Water Collection
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MaterialsDescriptionOrdering InformationNotes
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Disposable GlovesWorn throughout protocol from water collection through cleaning
Any brand will do; Kimberly-Clark gloves are recyclable by their home facility, however
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Field Notebook + PenRecords sampling time, location, depth, volume filtered and technician name on shift
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Whirlpak BagsHolds 1L of water from NiskinNasco: B01451
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2L Wide Plastic BottlesFor holding Whirlpaks upright during water sampling and filteringFisher Scientific: 11-815-11ACase of 6
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SharpiesLabeling of Whirlpak bags with water ID
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Milk Crate(s)Useful for transporting multiple full water samples from CTD back to lab.Amazon: Plastic Milk Crate 16qt
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Pump Setup
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MaterialsDescriptionOrdering InformationNotes
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Peristaltic Pump + Pump Heads PumpCole Palmer: Controler 7553-71
Cole Palmer: Pump head 77202-60
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Peristaltic Pump TubingPump tubingMasterFlex Tubing EW-96419-25Cole Palmer
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Swinnex Filter CartridgesHolds Filter; consists of top, bottom, and o-ringFisher Scientific: SX0002500
Clean in 10% bleach with 3x water rinse after use; let dry
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Luer Lock FittingsAttaches Filter Cartridge to tubingEW-45505-08 Cole Palmer
(25 pack) Order enough to put an Luer Lock on each piece to pump tubing
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Hose ClampsPrevents leaking of water at tube/cartridge junctionsEW-06832-03 Cole Palmer
(100 Pack) Secure's Luer Lock to pump tubing
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Cable TiesLabels tubes at each end (water sample and filter attachment) to ensure proper filter ID
Small Multicolored cable ties from any hardware store. These help color code the pump tubing to help sort out which tube is going into which water sample
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Disposable Lab PipettesAttach to the ends of pump tubing; directly goes into water sampleFisher Scientific: 13-676-10F
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1L Plastic BottlesPlastic bottles to measure filter outflowFisher Scientific: 07-201-428Case of 24
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Small plastic tubingSmall plastic tubing to connect filter outflow from filter cartridge end to plastic bottle openingCole Palmer:EW-96400-16
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eDNA Filter and Preservation
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MaterialsDescriptionOrdering InformationNotes
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Liquid Nitrogen DewareDNA Storage
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Millipore 0.22uM FilterseDNA filter, 1 per water sampleFisher Scientific: GVWP02500
Be careful to not include blue paper seperating filters in filter cartridges, this will stop filtering
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Razor BladesTo help pop open filter cartridge box; be careful!
Can insert razor blade in corners of unopened box to gently wedge open each side, otherwise box can be difficult to open without spilling filters
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20mL SyringeTo blow out excess water from filterFisher Scientific: 14-840-30
needs to have Luer-lock tipß
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2mL Cryovial TubesHolds eDNA filter after sampling for preservationThomas Scientific: 1154P75
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Cryovial LabelsTo label cryovials prior to samplingFisher Scientific: NC9650579
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Cryovial Tube RackTo organize cryovials prior to samplingUSA Scientific: 2331-5200
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ForcepsMust be cleaned between filters, used for placing filter into labeled cyrovial and also in cartridge prepMillepore: XX6200006P
Blunt end Forceps work best for eDNA filters, 3 or 4 pairs will do. Fine tip forcepts for pulling cotton swabs out of serological pipettes, 1-2 pairs.
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50mL Falcon TubesFalcon tubes will hold the following reagent washes in sequential order: 1x RNase Away, 2x MilliQ water, 1x 70% ethanolFisher Scientific: 352070
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KimwipesTo dry forceps after cleaning and prior to use on filterFisher Scientific: 34155
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Cleaning and General Lab Supplies
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MaterialsDescriptionOrdering InformationNotes
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BleachGeneral application: 10% solution for cleaning equipmentFisher Scientific: NC9724348
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EthanolGeneral application: 70% solution for cleaning equipment
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Bench PadsTo cover lab bench for sterile samplingFisher Scientific: 1420662
Change out daily
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RNase AwayUsed in cleaningThermofisher: 14-754-34
Skin irritant; some are more sensitive than others, so use gloves + longsleeves when handling
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Paper TowelsUsed in cleaning and laying out items to dry
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Bleach / Disinfectant WipesUsed in cleaning
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Autoclave BinsUsed in cleaning and laying out items to dryThermofisher: 6902-1000PK
Really, any large bin will do - these are just to hold materials during cleaning
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Ziploc BagsFor storing extra materials; may also be used in lieu of Whirlpaks…in a pinch
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Lab CoatFor dealing with bleach
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Eye ProtectionFor dealing with bleach - safety goggles, preferably
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MilliQ / DI WaterFor use in cleaning and in taking "collection blanks" each day
If not available on boat, bring a generous amount from a laboratory
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Thin forcepsFor removing cotton end from Disposable Lab PipettesFisher Scientific: 16-100-127
Long thin forceps
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Plastic TupperwareUsed in cleaning (can fill with filter cartridge pieces and shake to wash with different rinses)
example: https://ziploc.com/en/Products/Containers/Rectangle/Containers-Large-Rectangle
When shaking, either wash tops and bottoms seperately or frequently unscrew ones that have attached to ensure cleaning of all surfaces
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Plastic Graduated Cylinder (1L)To make Bleach solutions for cleaning
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Medium plastic bucketOptional, useful for holding used dirty cartridge pieces prior to cleaning
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