FRETbiosensors

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S
1	色分け	作成中	使用禁止	予定	よく動くものは赤字で反転
2	Bank No.	MAXI	Date	Name	Target or Name	backbone	FP for N terminus	phospho-binding domain	substrate	substrate sequence	linker	FP for C terminus	targetting signal	additional features	constructed by	開発コード	comment 1	comment 2	comment 3

3			2017/03/30	pGM-001	ERK	pPBbsr2-3594NLS	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	NLS	ERK docking domain(FQFP) in the substrate	maryu
4			2017/03/30	pGM-002	ERK	pPBbsr2-3594NLS	Ypet	WW (human PIN1)	RSK1 Turn motif (T359, S363)	RTPKDSPGIPP	116 a.a linker (EV linker)	SECFP	NLS	ERK docking domain(FQFP) in the substrate	maryu
5			2017/04/16	pGM-003	ERK	3555NES	Ypet	FHA1	RSK1 Turn motif (T359, S363)	RTPKDSPGIPP	116 a.a linker (EV linker)	ECFP	NES	ERK docking domain(FQFP) in the substrate	maryu
6			2017/04/16	pGM-004	ERK	3550NES	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	ECFP	NES	ERK docking domain(FQFP) in the substrate	maryu
7			2017/06/20	pGM-005	ERK	pCAGGS-MTS-bFd-linker-bFnr-BFP	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	なし		maryu		バイオセンサー部分をpCAGGSベクターに入れ，局在シグナルはなし
8			2017/06/22	pGM-006	ERK	pGM-005	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	tubulin alpha 1		maryu
9			2017/06/22	pGM-007	ERK	pGM-005	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Shugoshin 1		maryu
10			2017/06/22	pGM-008	ERK	pGM-005	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Lamin B		maryu
11			2017/06/21	pGM-009	ERK	pCAGGS-Flag-AktPH-m1V-X / pGM-001	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	KRas-CAAX		maryu		KRasCAAXを使うためにEcoRI, XbaIで入れ替え
12			2017/06/22	pGM-010	ERK	pGM-005	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Histone H1		maryu
13			2017/06/22	pGM-011	ERK	pGM-005	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Histone H2B (dEcoRI)		maryu
14			2017/06/26	pGM-012	ERK	pPBbsr-EAv2/pGM-009	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	KRas-CAAX		maryu
15				pGM-013	ERK	pPBbsr2-MCS	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	なし		maryu		pCAGGSからpPBbsr2にEcoRI, SalIで乗せ換える
16				pGM-014	ERK	pPBbsr2-MCS	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	tubulin alpha 1		maryu			EcoRI, SalIで入れ替え可能
17				pGM-015	ERK	pGM-013	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Shugoshin 1		maryu			制限酵素での切り出しが難しい。ペンディング
18				pGM-016	ERK	pGM-013	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Lamin B		maryu			pGM008からPCRで増やしてInfusion
19				pGM-017	ERK	pGM-013	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Histone H1		maryu			pGM-010と同じくプラスミドからPCRで増やしてInfusion
20				pGM-018	ERK	pGM-013	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	Histone H2B (dEcoRI)		maryu			pGM-010と同じくプラスミドからPCRで増やしてInfusion
21				pGM-019	ERK	pGM-013	Ypet	WW (human PIN1)	RSK1 Activation Loop (T573)	LLMTPCYT	116 a.a linker (EV linker)	SECFP	HEC1		maryu		結構でかい。C末につける。	SalIサイトの後ろにInfusionで入れる。フレームシフトに注意
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