iPSC room stock and maintenance

	A	B	C	D	E	F	G	H
1	For Coating
2	Reagent name	No. of unopened stock	Currently in order sheet	Date last ordered	Person who last ordered	Cat #	Location	Lot no.
3	DMEM/F12	1	4			Gibco 11320033	TC 4C
4	DMEM/F12 without phenol red		4			Gibco 21041025	TC 4C
5	Cultrex						iPSC room -20C freezer (aliquoted), freezer#3 -80C Rack 1 (2 boxes)
6	Phenol-Red Free Matrigel	6				Corning 356237	TC room -20C freezer
7	Matrigel gfr	2
8	matrigel hesc	0	3
9	PDL	2
10	For passaging/plating cells
11	Reagent name	No. of unopened stock	Currently in order sheet	Date last ordered	Person who last ordered	Cat #	Location	Lot no.
12	StemMacs	4				miltenyibiotec 130-104-688	RT (lower rack)
13	Accutase	6				stem cell, # 07922	iPSC room -20C (Aliquoted)
14	Dispase	4
15	For iPSC
16	Reagent name	No. of unopened stock	Currently in order sheet	Date last ordered	Person who last ordered	Cat #	Location	Lot no.
17	mTeSR Plus	1				stem cell, # 100-0276	iPSC room 4C (1 bottle), TC 4C (2), cold room
18	mTeSR Plus 5x supp	5				stem cell, # 100-0276	TC -20C
19	1% P/S bottles	3				15140122	iPSC room -20C (aliquoted)
20	LipofectamineSTEm	1				15140122
21	For pre-differentiation & differentiation neurons, imicroglia
22	Reagent name	No. of unopened stock	Currently in order sheet	Date last ordered	Person who last ordered	Cat #	Location	Lot no.
23	stempro	1	1
24	e8	1	1
25	xvivo		1
26	DMEM/F12 KO	1	4			12660012	TC 4C
27	CTS DMEM/F12 KO	1
28	Neurobasal A	5				10888022	TC 4C
29	N2	4				Gibco, 17502048	iPSC room -20C
30	MEM NEAA	1				Gibco 11140-050	iPSC room -20C
31	Glutamax	1					RT
32	B27	6				Gibco, 17502048	iPSC room -20C
33	BNDF	1				76303-662	iPSC room -20C
34	NT3	0	1			PHC7036	iPSC room -20C
35	Laminin	2					Freezer#3 -80C
36	Doxycycline	2					iPSC room 4C
37	Y compound	1					iPSC RT
38	Optimem	1
39	For freezing & thawing cells
40	Reagent name	No. of unopened stock	Currently in order sheet	Date last ordered	Person who last ordered	Cat #	Location	Lot no.
41	mFreSR	1					iPSC room -20C
42	TZV (rock inhibitor)	1					iPSC room -20C
43
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45	Others
46	Reagent name	No. of unopened stock	Currently in order sheet	Date last ordered	Person who last ordered	Cat #	Location	Lot no.
47	Cell lifters	1					RT
48	Trypan blue	1 opened vial					RT
49	PBS	0, shared with main TC					RT
50	Countless slides	4 boxes					RT
51	PDL lypholized	1					TC -20C
52	optiMEM	1					iPSC 4C
53	10 cm dish	1 opened box					RT
54	6 cm dish	1					RT
55	water	3					RT
56	pure 200 pipet tips	2 boxes					RT	vwr-72830-456
57	Individually wrapped 5mL						RT
58	Individually wrapped 10mL						RT
59	Individually wrapped 25mL						RT
60	Individually wrapped 2mL						RT
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65	stock = unopened
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