| A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | AA | |
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1 | experimentalist | date | time | project | experimentType | experimentalID | task | instrument | outcome | dataLocation | completed(n/y) | nextStep | nextStepDate | materialUsed | extra note | animal_inventory_filledOut | tissue_inventory_filledOut | IHC_form_filledOut | imaging_form_filledOut | orderingNeeds | protocols_References | ||||||
2 | nick | 1/7/24 | 5:00 PM | SANGs | reconstitution | sangs_010724_1 | reconstituted siRNA EGFR in labeling buffer at 100uM | none | aliquoted into 2 100ul @ 100uM 2 used for 647 labeling rx | fridge | yes | na | na | siRNA lot number: ASO2L783 refNum: 4390826 | 1 will be the unlabeled I give to Elisa from Dahlman lab | na | na | na | na | ||||||||
3 | nick | 1/7/24 | 5:00 PM | SANGs | conjugation | sangs_010724_2 | conjugate siRNA EGFR to AF647 dye via Ulysis Rx | thermocycler | conjugated 200ul of 100uM siRNA with all of the 647 kit | fridge | yes | clean up with p30 gel colum | done | ulysis 647 kit and 200uL of EGFR siRNA from sangs010724_1 | final concentration is diluted down to 66.7uM | na | na | na | na | https://drive.google.com/file/d/1-2WWJylxzClJBQehxLegXzt3xMdhywB8/view?usp=share_link | |||||||
4 | nick | 1/8/24 | 9:00 AM | SANGs | biodistribution | sangs_010824_1 | iv delivery 5mg 488-sangs to p7 | none | jugular delivery with saline flush | none | yes | evaluate tissue ditribution | na | sangs-488 with saline lot number fk8409 | 5mg delivered | yes | na | na | na | ||||||||
5 | nick | 1/8/24 | 11:30 AM | neuroTumor | imaging | neuroTumor_010824_1 | image HEY cells 24h post lenti-gfp transfection | 900c | cells transfected | onedrive | yes | na | na | 5ul lenti GFP | na | na | na | na | na | ||||||||
6 | jing | 1/8/24 | 3:00 PM | neuroTumor | imaging | neuroTumor_010824_1 | image ovary slices | 900B | neurofilament and TUJ1 did not colocalize | onedrive | yes | test with different Ab | slide 1 (TUJ1_647; TH_cy3; Neurofilament_FITC), slide 2 (TUJ1_647; PGP 9.5_cy3; Neurofilament_FITC); 1:500, primary overnight, wash 3 times, secondary overnight, wash 3 times | na | na | na | na | na | |||||||||
7 | jing | 1/9/24 | 3:00 PM | neuroTumor | genotyping | neuroTumor_010924_1 | genotyping 50 mice m119-m168 | DAR Prep Room | listed on animal mapping sheet | animal mapping sheet | yes | waiting for response from Transnetyx | na | na | na | na | na | na | |||||||||
8 | jing | 1/10/24 | 3:00 PM | neuroTumor | dissection | m170_011024_1 | dissect sympathetic nervous system, ovary m 169 | rig room 2 | failed to find sympathetic, found spinal nerves | none | yes | do another one | CD-1 female m169 | na | na | na | na | na | |||||||||
9 | nick | 1/11/24 | 3:00 PM | CTCs | imaging | ctcs_011124_1 | image CTCs on filter | 900c | 2-3 good images particularly on 63x | 900c | yes | na | na | na | na | na | na | na | yes | ||||||||
10 | jing | 1/11/24 | 2:00 PM | neuroTumor | dissection | m170_011124_1 | dissect sympathetic nervous system, ovary, sc, brain m 170 | rig room 2 | yellow part (sym trunk) extend towards the center towards the caudal direction, collect ovary, sc, brain, sym trunk (l, m, r) | fridge (hood side) | yes | sucrose | CD-1 female m 170 | na | na | na | na | na | |||||||||
11 | nick | 1/12/2024 | 3:00 PM | neuroTumor | dissection | m0087_011224_1 | dissection | na | r ovary isolated and l ovary on FT | RT rocker | no | move to sucrose for sectioning 1/13 ot 1/14 | done | 4%PFA | na | yes | yes | na | na | ||||||||
12 | nick | 1/12/2024 | 3:00 PM | neuroTumor | dissection | m0088_011224_2 | dissection | na | r ovary isolated and l ovary on FT | RT rocker | no | move to sucrose for sectioning 1/13 ot 1/14 | done | 4%PFA | na | yes | yes | na | na | ||||||||
13 | nick | 1/12/2024 | 3:00 PM | neuroTumor | dissection | m0089_011224_3 | dissection | na | r ovary isolated and l ovary on FT | RT rocker | no | move to sucrose for sectioning 1/13 ot 1/14 | done | 4%PFA | na | yes | yes | na | na | ||||||||
14 | jing | 1/12/2024 | 1:00 PM | neuroTumor | IHC | neuroTumor_011224_1 | slicing m169 ovary 50 um, block 1hr, primary overnight | Leica, wellplate with strainer | 50 um floating section | none | yes | IHC | Leica, 10% BSA, PGP 9.5 1:500, Neurofilament 1:500 | na | yes | yes | yes | no | TH | ||||||||
15 | jing | 1/12/2024 | 12:30 PM | neuroTumor | weaning | neuroTumor_011224_2 | weaning litter from phox2b_2 & tdTom_5 | na | Phox2b-tdtomato cage 7 | mouse breeder housing | yes | none | none | na | no | no | na | na | |||||||||
16 | jing | 1/12/2024 | 12:00 PM | neuroTumor | sucrose | neuroTumor_011224_3 | m170 sym trunk, sc, brain, ovary equilibrate in sucrose overnight | na | equilibrium | fridge (hood side) | yes | slicing, IHC | sucrose solution | na | yes | yes | no | no | |||||||||
17 | jing | 1/13/24 | 11:00 AM | neuroTumor | IHC | neuroTumor_011324_1 | wash 3*20min, secondary overnight | wellplate with strainer | finished | none | yes | IHC | 1X PBS, chic fitc 1:500, rb cy3 1:500 | na | yes | yes | yes | no | |||||||||
18 | nick | 1/14/24 | 11:00 AM | SANGs | cell transfection | sangs_011424_1 | aliquot mRNA mCherry | na | 500ng/ul (down from 1000ng/ul or 1mg/ml) 20x5ul 5x10ul 2x25ul | -80 | yes | na | trilink mRNA mCherry 100ug | na | na | na | na | na | |||||||||
19 | nick | 1/14/24 | 3:00 PM | SANGs | cell transfection | sangs_011424_2 | transfect mRNA mcherry | na | transfected 1- 4 well @100ng 2- 4 well @500ng 3- NG alone 4- NG+mRNA low 5- NG+mRNA high 6- rAAV mcherry 7- gcamp 8- gcamp 9- empty mRNA mCherry | nones=1. | no | image | 2x 5ul aliquots 1x10ul aliquots 1x25ul aliquots | na | na | na | na | na | https://drive.google.com/file/d/1oSGRIq5UfmLY26ohqEXiRr0BKh4Dq-n1/view?usp=share_link | ||||||||
20 | jing | 1/14/24 | 6:00 PM | neuroTumor | IHC | neuroTumor_011424_1 | wash 3*20min, mount | wellplate with strainer | mounted | fridge (hood side) | yes | image | 1X PBS, vector shield | na | yes | yes | yes | no | |||||||||
21 | jing | 1/14/24 | 6:30 PM | neuroTumor | procedure card | neuroTumor_011424_2 | add genotype procedure card for all litters | none | done | mouse breeder housing | yes | none | proceudre card | na | yes | yes | na | na | |||||||||
22 | jing | 1/15/24 | 12:00 PM | neuroTumor | IHC | m170_011524_1 | m170 sym trunk (r, m, l) slicing 50 um, block 1hr, primary overnight | Leica, wellplate with strainer | 50 um floating section | none | yes | IHC | Leica, 10% BSA, TH 1:500, Neurofilament 1:500, TUJ1 1:500 | na | yes | yes | yes | no | |||||||||
23 | nick | 1/16/24 | 3:00 PM | SANGs | tumor induction | m171 to m175 | heya8f8 cell induction 500k r LQ | na | 5 dosed | none | yes | 12 days check BLI | 500k hey cells per animal | na | yes | na | na | na | |||||||||
24 | jing | 1/16/24 | 12:00 PM | neuroTumor | IHC | m170_011624_1 | m170 sym trunk (r, m, l), wash 3*2o min, secondary overnight | wellplate with strainer | done | none | yes | IHC | 1X PBS, chic fitc 1:500, rb cy3 1:500 | na | yes | yes | yes | no | |||||||||
25 | jing | 1/16/24 | 1:00 PM | neuroTumor | dissection + post-fix | m176_011624_1, m177_011624_1 | dissect ovary, sym trunk | na | in post-fix | shaker | yes | sucrose | 4% PFA | na | yes | yes | no | no | |||||||||
26 | jing | 1/16/24 | 4:00 PM | neuroTumor | dissection + post-fix | m112_011624_1, m125_011624_1 | dissect ovary, pancreas, sym trunk, sc, brain | na | in post-fix | shaker | yes | sucrose | 4% PFA | na | yes | yes | no | no | |||||||||
27 | jing | 1/17/24 | 8:30 AM | neuroTumor | survival sugery | m158_011724_1, m162_011724_1, m163_011724_1, m164_011724_1, m167_011724_1, m168_011724_1 | adeno-cre injection in right ovary (f) or pancreas (m) | OR | done | ABSL-II room | yes | cancer development | adeno-cre vector withh methylene blue 10 ul | na | yes | yes | no | no | |||||||||
28 | jing | 1/17/24 | 5:00 PM | neuroTumor | dissection | m178_011724_1, m179_011724_1 | ovary dissection | na | done | runner | yes | sucrose | 4% PFA | na | yes | yes | no | no | |||||||||
29 | jing | 1/17/24 | 2:00 PM | neuroTumor | IHC | m170_011724_1 | add nissil | na | shake overnighht | shaker | yes | IHC | nissil 1:500 | na | yes | yes | yes | no | |||||||||
30 | olivia | 1/18/2024 | 3:00 PM | neuroTumor | dissection | m180_011824_1 | dissect DRG | rig room 2 | done | none | yes | culture cells | dissection tools, fume hood, isoflurane, | na | yes | yes | yes | no | |||||||||
31 | olivia | 1/18/24 | 4:00 PM | neuroTumor | culture | m180_011824_2 | culture DRG | Incubator | growing | inucbator | yes | grow cells | Incubator, fume hood, R10 media | na | yes | yes | yes | no | |||||||||
32 | jing | 1/18/24 | 2:00 PM | neuroTumor | IHC | m170_011724_1 | wash 20min *3 | shaker | done | slide in fridge neat hood side | yes | imaging | 2/2/24 | na | na | yes | yes | yes | no | ||||||||
33 | jing | 1/18/24 | 5:00 PM | neuroTumor | dissection | m136_011824_1 | ovary, sc, brain, nodose dissection | rig room 2 | in post-fix | runner | yes | sucrose | 4% PFA | na | yes | yes | no | no | |||||||||
34 | jing | 1/19/24 | 10:00 AM | neuroTumor | survival sugery | m123_011924_1, m131_011924_1, m143_011924_1, m145_011924_1 | lentivirus injection in right ovary | OR | done | ABSL-II room | yes | cancer development | lentivirus cocktail with methylene blue 10-15 ul right ov | na | yes | na | na | na | |||||||||
35 | jing | 1/19/24 | 3:00 PM | neuroTumor | dissection | m138_011924_1 | sc, brain, nodose dissection | rig room 2 | in post-fix | runner | yes | sucrose | 4% PFA | na | yes | na | na | na | |||||||||
36 | jing | 1/20/24 | 12:00 PM | neuroTumor | IHC | m176_012024_1 | slicing 50 um, blocking, primary | cryostat | overnight | slide | yes | IHC | NDS, TH, Neurofilament | na | yes | yes | yes | no | |||||||||
37 | jing | 1/21/24 | 12:00 PM | neuroTumor | IHC | m176_012024_1 | wash, secondary+nissl | na | overnight | slide | yes | mount | cy3, 647, nissl | na | yes | yes | yes | no | |||||||||
38 | jing | 1/22/24 | 11:00 AM | neuroTumor | IHC | m176_012024_1 | wash 20min *3 | na | done | slide | yes | imaging | PBST | na | yes | yes | yes | no | |||||||||
39 | nick | 1/22/24 | 3:00 PM | neuroTumor | tumor check | m171 to m175 | IVIS check | IVIS | m171, m172, m173 and m175 have tumors but low induciton on m174 | onedrive | yes | check 1 week | 1/28/24 | d-luciferin | na | na | na | na | yes | ||||||||
40 | jing | 1/22/24 | 1:00 PM | neuroTumor | tissue sectioning | m112_012224_1, m125_012224_1, m136_012224_1, m138_012224_1 | slicing brain, sc, sym | cryostat | on slide | neurocancer slide box 1 | yes | imgaing | none | na | yes | yes | na | no | |||||||||
41 | jing | 1/23/24 | 10:00 AM | neuroTumor | tissue clear | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | dehydration: 0&4 MeOH in water gradient, 1&2&3 MeOH in B1N gradient | runner | done | runner | no | rehydration: 0&4 MeOH in water gradient, 1&2&3 in B1N gradient | MeOH, DI water, B1N | na | yes | yes | na | no | |||||||||
42 | jing | 1/24/24 | 12:00 PM | neuroTumor | tissue clear | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | rehydration | runner | done | runner | no | wash | MeOH, DI water, B1N | na | yes | yes | na | no | |||||||||
43 | jing | 1/24/24 | 5:30 PM | neuroTumor | dissection + post-fix | m134_012424_1 | ovary, pancreas dissection + post-fix | rig room 2 | done | runner | yes | sucrose | 4% PFA | na | yes | yes | no | no | |||||||||
44 | jing | 1/24/24 | 2:00 PM | neuroTumor | tissue sectioning | m136_012424_1 | ovary, pancreas slicing | cryostat | done | slide | yes | imgaing | none | na | yes | yes | na | no | |||||||||
45 | nick | 1/24/24 | 5:00 PM | CTCs | confocal | ctcs_012524_1 | image fliter with Hey gfp cells | 900d | done | onedrive in aref nick shared folder | yes | na | na | none | na | na | na | na | yes | ||||||||
46 | nick | 1/25/24 | 3:00 PM | neuroTumor | survival sugery | m185-m189 | right ovary induction with Hey-A8-F8-gfp | OR | done | none | yes | ivis in one week | 2/1/24 | new hey-a8-f8-gfp cells | na | yes | na | na | na | ||||||||
47 | jing | 1/25/24 | 11:00 AM | neuroTumor | sucrose | m134_012424_1 | change into sucrose | na | runner | runner | yes | slicing, IHC | sucrose solution | na | yes | yes | na | na | |||||||||
48 | jing | 1/25/24 | 11:30 AM | neuroTumor | tissue clearing | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | wash, blocking overnight | runner | done | runner | no | IHC | PBxwT, 10% NDS | na | yes | yes | na | na | |||||||||
49 | jing | 1/25/24 | 1:00 PM | neuroTumor | survival sugery follow-up | m158_011724_1, m162_011724_1, m163_011724_1, m164_011724_1, m167_011724_1, m168_011724_1, m123_011924_1, m131_011924_1, m143_011924_1, m145_011924_1 | new skin or clamp fix | PrepR | done | ABSL-II room | yes | na | new skin, clamp, saline | na | yes | yes | na | na | |||||||||
50 | jing | 1/25/24 | 3:00 PM | neuroTumor | genotyping | m190-m208_012524_1 | genotype 19 mice | PrepR | done | none | yes | na | na | na | yes | na | na | na | |||||||||
51 | jing | 1/25/24 | 5:00 PM | neuroTumor | sucrose | m87-89_012524_1 | change into sucrose | runner | done | runner | yes | slicing, IHC | na | na | yes | yes | no | no | |||||||||
52 | jing | 1/26/24 | 12:00 PM | neuroTumor | tissue clearing IHC | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | primary | runner | equilibrate | runner | no | wash | NFH, TH, CHat 1:500 | na | yes | yes | yes | na | |||||||||
53 | jing | 1/26/24 | 1:00 PM | neuroTumor | tissue sectioning | m134_012624_1 | slicing pancreas, ovary 50 um, mount | cryostat | done | slide | yes | imaging | na | na | yes | yes | na | yes | |||||||||
54 | nick | 1/26/24 | 1:00 PM | SANGs | biodistribution | p1 through p4 | iv dosing 488 sangs | na | done | in pig | yes | ex vivo confirmation with ivis and slice | ng488010724 | 4mg total dose | yes | na | na | na | |||||||||
55 | nick | 1/27/24 | 8:00 AM | oncopig | MRI | p1 through p4 | abdomino-pelvic MRI | CABI 3T | MRI confirmed tumors in 3 pigs | in oncopig folder one drive and NAS | yes | na | na | 4ml gadolinium per pig | na | yes | na | na | yes | ||||||||
56 | nick | 1/29 | 8:00 AM | oncopig | terminal | p1 through p4 | necropsy | na | all tissues in 10%NBF tumor confirmed in 3/4 | T3 in 10%nbf | no | pick up tissues | 2/1/24 | na | na | yes | yes | na | na | ||||||||
57 | jing | 1/29/24 | 12:00 PM | neuroTumor | tissue sectioning | m125_012924_1, m134_012924_1, m136_012924_1 | brain, sc, ov, pancreas, nodose, sym trunk 50um | cryostat | done | slide | yes | IHC | na | none | na | yes | yes | yes | na | ||||||||
58 | jing | 1/29/24 | 12:30 PM | neuroTumor | IHC | m112_012224_1, m125_012224_1, m136_012224_1, m138_012224_1, m134_012424_1 | take off coverlip, primary | na | done | slide | yes | IHC | na | dsred | na | yes | yes | yes | no | ||||||||
59 | jing | 1/29/24 | 6:00 PM | neuroTumor | IHC | m125_012924_2, m134_012924_2, m136_012924_2 | block, primary | na | done | slide | yes | IHC | na | dsred, TUJ1, NFH | na | yes | yes | yes | no | ||||||||
60 | jing | 1/30/24 | 12:00 PM | neuroTumor | IHC | m112_012224_1, m125_012224_1, m136_012224_1, m138_012224_1, m134_012424_1 | wash, secondary, wash, mount | na | done | slide | yes | IHC | na | PBST | na | yes | yes | yes | no | ||||||||
61 | jing | 1/30/24 | 5:00 PM | neuroTumor | IHC | m112_012224_1, m125_012224_1, m136_012224_1, m138_012224_1, m134_012424_1 | imaging check | rig room 2 microscope | done | slide | yes | na | na | na | |||||||||||||
62 | jing | 1/30/24 | 12:00 PM | neuroTumor | IHC | m125_012924_2, m134_012924_2, m136_012924_2 | wash, secondary | na | done | slide | yes | IHC | na | PBST, dsred-cy3, TUJ1-647, NFH-FITC | na | yes | yes | yes | no | ||||||||
63 | nick | 1/30/23 | 3:00 PM | neuroTumor | terminal | m0095 through m0097 | terminal dissection | IVIS room | no obvious tumors but m0095 had tdTomato expression on IVIS imaging | m0095 | yes | IHC | slice | 4%pfa | yes | ||||||||||||
64 | jing | 1/30/24 | 3:30 PM | neuroTumor | survival surgery follow-up | m158_011724_1, m162_011724_1, m163_011724_1, m164_011724_1, m167_011724_1, m168_011724_1, m123_011924_1, m131_011924_1, m143_011924_1, m145_011924_1 | clamp off, new skin apply | PrepR | done | ABSL-II room | yes | na | na | na | na | yes | na | na | na | ||||||||
65 | jing | 1/31/24 | 12:00 PM | neuroTumor | IHC | m125_012924_2, m134_012924_2, m136_012924_2 | wash | na | done | slide | yes | imaging | na | PBS | na | yes | yes | yes | no | ||||||||
66 | jing | 1/31/24 | 1:00 PM | neuroTumor | tissue clear IHC | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | wash | incushaker | done | incushaker | yes | IHC | na | PBST | na | yes | yes | yes | na | ||||||||
67 | jing | 2/1/24 | 3:00 PM | neuroTumor | tissue sectioning | m97_020124_1 | left and right ovary slicing 50 um | cryostat | done | -20 (hood side) | yes | IHC | na | na | |||||||||||||
68 | jing | 2/1/24 | 12:00 PM | neuroTumor | tissue clear IHC | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | secondary | incushaker | done | incushaker | no | IHC | na | NFH-488/fitc, TH-cy3, CHat-647 1:500 | na | yes | yes | yes | no | ||||||||
69 | william | 2/2/2024 | 1:00 PM | neuroTumor | imaging | m0083_020224_1 | confocal imaging of ovary with NfH and pgp9.5 | 900d | done | onedrive | yes | na | na | na | na | na | yes | ||||||||||
70 | olivia | 2/2/2024 | 3:00 PM | neuroTumor | dissection | m209_020224_1 | dissect 13 DRGs | rig room 2 | done | na | yes | culture | 2/2/24 | dissection tools, fume hood, isoflurane | na | yes | yes | no | no | ||||||||
71 | olivia | 2/2/2024 | 4:00 PM | neuroTumor | culture | m209_020224_2 | culture DRG | incubator | in progress | incubator | no | fix | 2/6/24 | incubator, fume hood, R10 media | na | yes | yes | yes | yes | ||||||||
72 | jing | 2/2/2024 | 1:00 PM | neuroTumor | euthanize | na | ethanize CD-1 litter from foster | CO2 | done | na | yes | na | na | CO2 | na | na | na | na | na | ||||||||
73 | jing | 2/2/2024 | 2:00 PM | neuroTumor | survival surgery follow-up | m123_011924_1, m131_011924_1, m143_011924_1, m145_011924_1 | clamp off, new skin apply | PrepR | done | ABSL-II room | yes | na | na | na | na | yes | yes | na | na | ||||||||
74 | nick | 2/3/24 | 9:00 AM | SANGs | ivis | m185-m189 | check tumor induciton 9 days post r ovary injeciton of Hey | IVIS | all have in vivo detectable tumors | onedrive | yes | transcardial perfusion | 2/4/24 | d-luciferin | m185 had measurably smaller tumors | yes | na | na | yes | ||||||||
75 | nick | 2/3/24 | 11:00 AM | neuroTumor | ivis | m171 to m175 | check tumor induciton 18 days post IP injeciton of Hey | IVIS | all have in vivo detectable tumors | onedrive | yes | treat with Elisa LNP and SANGs | 4/8/24 | d-luciferin | m173 and m174 had smaller tumors, they were imaged independently | yes | na | na | yes | ||||||||
76 | nick | 2/3/24 | 12:00 PM | neuroTumor | ivis | m164 | check gfp in pancreas and palpation | IVIS | there does look to be hot spot on left flank of GFP which coincides with palpable solidification | onedrive | yes | check in 1 weel | 2/10/24 | na | na | yes | na | na | yes | ||||||||
77 | nick | 2/3/24 | 12:00 PM | neuroTumor | ivis | m167 | check gfp in pancreas and palpation | IVIS | there does look to be hot spot on left flank of GFP which coincides with palpable solidification | onedrive | yes | check in 1 weel | 2/10/24 | na | na | yes | na | na | yes | ||||||||
78 | nick | 2/3/24 | 1:00 PM | neuroTumor | ivis | r226 | check tumor induciton | IVIS | large R lower lung tumor | onedrive | yes | transcardial perfusion | 2/4/24 | d-luciferin | na | yes | na | na | yes | ||||||||
79 | jing | 2/5/2024 | 12:00 PM | neuroTumor | euthanize | m113_020524_1, m119_020524_1, m120_020524_1, m122_020524_1, m129_020524_1, m140_020524_1, m141_020524_1, m153_020524_1, m157_020524_1 | ethanize cre negative males | CO2 | done | na | yes | na | na | CO2 | na | yes | na | na | na | ||||||||
80 | jing | 2/5/2024 | 2:00 PM | neuroTumor | IHC | m97_020124_1 | block, primary | slide | incubate overnight | slide | yes | IHC | 02/06/2024 | NDS, PGP 9.5, TH | na | yes | yes | yes | no | ||||||||
81 | jing | 2/6/24 | 12:00 PM | neuroTumor | tissue clear IHC | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | wash 3*1hr | incushaker | done | fridge (hood side) | yes | dehydration | na | PBST | na | yes | yes | yes | no | ||||||||
82 | jing | 2/6/24 | 12:10 PM | neuroTumor | IHC | m97_020124_1 | wash, secondary, wash, mount | slide | done | slide | yes | na | na | NFH, TH, CHat 1:500 | na | yes | yes | yes | no | ||||||||
83 | jing | 2/6/24 | 5:00 PM | neuroTumor | tissue sectioning | m209_020624_1 | brain, sc, 50um slicing | cryostat | done | slide | yes | wash and mount | na | na | na | yes | yes | na | no | ||||||||
84 | william | 2/6/24 | 3:00 PM | neuroTumor | imaging | m0082_02062024_1 | confocal imaging of ovary with NfH and pgp9.5 | 900b | done | onedrive | yes | yes | |||||||||||||||
85 | william | 2/6/24 | 3:00 PM | neuroTumor | imaging | m0082_02062024_2 | confocal imaging of ovary with NfH and S100 | 900b | done | onedrive | yes | na | na | na | na | na | yes | ||||||||||
86 | nick | 2/7/24 | 9:00 AM | neuroTumor | tissue sectioning | neuroTumor_020724_1 | section tumors for xenium | microtome A | done | on slide | yes | send to Duke | 2/9/24 | xenium slides | na | na | na | yes | na | ||||||||
87 | nick | 2/7/24 | 12:00 PM | SANGs | conjugation | sangs_020724_1 | conjugate siRNA EGFR to AF647 dye via Ulysis Rx | thermocycler | conjugated 200ul of 100uM siRNA with all of the 647 kit | fridge | yes | clean up with p30 gel colum | 2/7/24 | ulysis 647 kit rest of 100uL of EGFR siRNA from sangs010724_1 | final concentration is diluted down to 66.7uM | na | na | na | na | https://drive.google.com/file/d/1-2WWJylxzClJBQehxLegXzt3xMdhywB8/view?usp=share_link | |||||||
88 | jing | 2/7/24 | 11:00 AM | neuroTumor | tissue clear IHC | m176_012324_1, m176_012324_2, m177_012324_1, m177_012324_2, m178_012324_1 | dehydration | runner | done | runner | yes | clearing | na | 20%-100% MeOH in H2O | see adipose clear and iDisco+ protocols | yes | yes | na | na | ||||||||
89 | jing | 2/7/24 | 11:10 AM | neuroTumor | tissue sectioning | m209_020624_1 | wash OCT off, mount | slide | done | slide | yes | imaging | na | PBS | na | yes | yes | na | no | ||||||||
90 | jing | 2/7/24 | 4:00 PM | neuroTumor | dissection | r0243_020724_1 | 4% PFA perfusion, sc, tumor dissection | rig room 2 | done | shaker | yes | na | na | 4% PFA | na | yes | yes | na | na | ||||||||
91 | jing | 2/8/24 | 8:00 AM | neuroTumor | survival surgery | m117_020824_1, m118_020824_1, m147_020824_1, m148_020824_1 | tracer cocktail injection 5-10ul in right ov | OR | done | ABSL-II room | yes | na | na | fast blue, methylene blue, AAV2 | na | yes | na | na | no | ||||||||
92 | jing | 2/9/24 | 10:00 AM | neuroTumor | survival surgery follow-up | m158_011724_1, m162_011724_1, m163_011724_1, m164_011724_1, m167_011724_1, m168_011724_1 | tumor check | NPR | done | ABSL-II room | yes | na | na | ||||||||||||||
93 | william | 2/9/24 | 4:00 PM | neuroTumor | imaging | m0082_020924_1 | confocal imaging of ovary with NfH and TH | 900A | done | onedrive | yes | na | na | na | na | na | yes | ||||||||||
94 | jing | 2/12/24 | 2:00 PM | neuroTumor | dissection | m147_021224_1 | drg, sc, ovary, nodose, sympathetic trunk | fix tools | post-fix | runner | yes | sucrose | done | 4% PFA | na | yes | na | na | na | ||||||||
95 | jing | 2/13/24 | 11:00 AM | neuroTumor | dissection | m148_021324_1 | drg, sc, ovary, nodose, sympathetic trunk | fix tools | post-fix | runner | yes | sucrose | done | 4% PFA | na | yes | na | na | na | ||||||||
96 | olivia | 2/13/24 | 11:00 AM | neuroTumor | dissection | m210_021324_1 | DRG | rig room 2 | done | na | yes | culture | 2/13/24 | dissection tools, fume hood, isoflurane, | na | yes | yes | no | no | ||||||||
97 | olivia | 2/13/24 | 12:00 PM | neuroTumor | cell culture | m210_021324_1 | DRG | incubator | in progress | na | yes | culture | 2/18/24 | Incubator, fume hood, R10 media | na | yes | yes | yes | yes | ||||||||
98 | william | 2/13/24 | 10:00 AM | neuroTumor | imaging | m83_021324_1 | confocal imaging of lung with NfH and S100 | 900A | done | onedrive | yes | na | na | na | na | na | na | na | yes | ||||||||
99 | william | 2/13/24 | 12:00 PM | neuroTumor | imaging | m83_021324_2 | confocal imaging of lung with NfH and PGP9.5 | 900A | done | onedrive | yes | na | na | na | na | na | na | na | yes | ||||||||
100 | william | 2/14/24 | 11:00 AM | neuroTumor | imaging | m83_021424_1 | confocal imaging of lung with NfH and TH | 900A | done | onedrive | yes | na | na | na | na | na | na | na | yes |