| A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Use this template for mastermix preparation. Make a new tab and rename it with the date. | ||||||||||||||||
2 | Who? | Björn | <-- | State your name | |||||||||||||
3 | Volume | 2000 | µL | <-- | change the volume if necessary | ||||||||||||
4 | Enzyme | Taq | Homemade | <-- | State which enzyme was used | ||||||||||||
5 | |||||||||||||||||
6 | PCRMastermix-2X | ||||||||||||||||
7 | | | Component | | | µL | | | stock | | | 2xmm | | | final | | | <-- | This is a markdown table | ||||
8 | | | ---------- | | | ----- | | | ------- | | | ------ | | | ------ | | | copy this table | |||||
9 | | | Water | | | 1260 | | | - | | | - | | | - | | | format it with | |||||
10 | | | Buffer(xC) | | | 400 | | | 10.00 | | | 2 | | | 1 | | | Markdown Table Formatter | |||||
11 | | | MgCl2(mM) | | | 160 | | | 50.0 | | | 4 | | | 2 | | | Copy it back to wikidpad | |||||
12 | | | dNTPs(mM) | | | 80 | | | 10.0 | | | 0.4 | | | 0.2 | | | The table can also be viewed on | |||||
13 | | | DMSO(100%) | | | 0 | | | 100% | | | 0.0% | | | 0.0% | | | StackEdit | |||||
14 | | | Taq | | | 100 | | | 100% | | | 5.0% | | | 2.5% | | | ||||||
15 | | | Total | | | 2000 | | | - | | | - | | | - | | | ||||||
16 | |||||||||||||||||
17 | See more about PCR | ||||||||||||||||
18 | protocol-online | ||||||||||||||||
19 | This mastermix was based on the description of FERMENTAS 2xmm: | ||||||||||||||||
20 | Taq DNA polymerase (0.05 U/µL), reaction buffer, 4 mM MgCl2, and 0.4 mM of each dNTP | ||||||||||||||||