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ABCDEFG
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P20015 mL conicalP20015 mL conical
2
Solution
# samples
Required Volume (μL)(μL) per sample
3
Methanol2200.02000100.001000
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.1% CF3COOH, 70% ACN in water
2100.0100050.00500
5
.1% CF3COOH in water4400.04000100.001000
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70% ACN in water2100.0100050.00500
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[ ]Desalting of Peeptides.
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[ ]Place a 3 ml strata C18 cartridge in and 15 ml conical tube
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[ ]Add 1 ml of CH3OH and centrifuge at 2000 x g for 1 min.
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[ ]Add 0.5 ml of 0.1% CF3COOH, 70% CH3CN in water and centrifuge at 2000 x g for 1 min.
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[ ] Add 0.5 ml of 0.1% CF3COOH in water and centrifuge at 2000 x g for 1 min.
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[ ]Load the filtrate and centrifuge at 1000 x g for 3 min
15
[ ]Add 0.5 ml of 0.1% CF3COOH in water and centrifuge at 1000 g for 3 min.
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[ ]Transfer the cartridge into a new tube, add 0.5 ml 70% CH3CN in water and centrifuge at 1000
g for 3 min.
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Stage Tip Solutions
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Buffers
BSA digestion
MBP