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The following fields are required to be the first four columns in the *.qptiff.channels.csv file (order matters). Data providers are welcome to include additional columns that further describe the channels. This file should include one row per channel in the accompanying QPTIFF file.
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Field NameField TypeDefinitionOptions:
required, optional
NOTESVALUES for CATEGORICAL FIELDS
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is channel used for nuclei segmentationis channel used for cell segmentationis antibodychannel quality
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channel idtextStructure of the identifier depends on the acquisition system. Whenever possible this should exactly match the channel ID in the QPTIFF file. For example the channel ID in an QPTIFF might be something like "Channel:0:13" which would then be the value entered here.requiredYesYesYesgood
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is channel used for nuclei segmentationbooleanSpecifies if this channel can be used by algorithms performing nuclei segmentation ("Yes" or "No").requiredIn data types for which a HIVE pipeline performs cell and nuclear segmentation, exactly one channel must have value "Yes".NoNoNofair
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is channel used for cell segmentationbooleanSpecifies if this channel can be used by algorithms performing cell segmentation ("Yes" or "No").requiredIn data types for which a HIVE pipeline performs cell and nuclear segmentation, exactly one channel must have value "Yes".Not applicableNot applicableNot applicablepoor
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is antibodybooleanDoes this channel encode image intensities associated with an antibody ("Yes" or "No")? If "Yes" then the relevant antibody is expected to be listed in the antibodies.tsv file with the same channel ID.required
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channel qualitycategoricalThis is a rough metric of the quality of the individual channel within the image. If any channels are denoted as "poor" quality, then the reason for including the poor channel(s) must be noted in the abstract for this dataset.optional
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default for displaybooleanThis denotes if this channel should be enabled by default in the data portal visuals.optional
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thresholdintegerThis denotes the maximum threshold value for this channel when being displayedoptional
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minimum thresholdintegerThis denotes the minimum threshold value for this channel when being displayed.optional
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