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SMILE: System for Marine Invertebrate Larval Expeirmentation - Putnam Lab, URI; Ariana Huffmyer, Jill Ashey
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PROTOCOL FOR ASSEMBLY:
https://docs.google.com/document/d/13hQXY1mEwzKhxbFFZCw6-jbcCd6CUVb2G1M6lSuEELI/edit
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NUMBER OF SYSTEMS: 2
1 system = cooler + 6 squaricals (1 per temperature treatment)
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*insert desired number of systems here
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ItemQuantity per system PurposeTotal Quantity Needed ManufacturerCat NumberLink to Purchase
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HEADER TANK
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Cooler (120 qt) 1Header tank2Igloo44577https://smile.amazon.com/gp/product/B004QILD6W/ref=ppx_yo_dt_b_search_asin_title?ie=UTF8&th=1
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Float valve 1Float valve for water input to header2Tractor Supply Co. 217032299
https://www.amazon.com/Trough-Matic%C2%AE-Stock-Water-Tank/dp/B000B4GTPK/ref=sr_1_3?crid=1UCWK3H0F8YNQ&keywords=float+valve+livestock+water+tank&qid=1660255178&sprefix=float+valve+livestock+water+tank%2Caps%2C184&sr=8-3&qty=4#
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¾" barb nylon fitting (garden hose thread)1Fitting for water tubing to float valve2Lowes877102https://www.lowes.com/pd/B-K-3-4-in-Barbed-Barb-x-Garden-Hose-Adapter-Fitting/1000505049
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Mag Drive pump (Model 2) 1Delivery pump2Danner MFG or PondMasterMag Drive 02512 Model 2https://dannermfg.com/products/supreme-aqua-mag-magnetic-drive-water-pumps
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TUBING & WATER DELIVERY
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¾" interior diameter tubing (10ft) 1Tubing for water input to cooler, from pump to manifold2Lowes879267https://www.lowes.com/pd/EZ-FLO-3-4-in-Inner-Diameter-x-10-ft-PVC-Clear-Vinyl-Tubing/1000365037
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½" interior diameter tubing (10ft)1Tubing for return valve from PVC manifold to cooler2Lowes814319https://www.lowes.com/pd/EZ-FLO-1-2-in-Inner-Diameter-x-10-ft-PVC-Clear-Vinyl-Tubing/1000180551
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1" diameter (outer tubing diameter) clamps2Secure tubing if needed to coolers/structures4Lowes1414578https://www.lowes.com/pd/HoldRite-5-Pack-1-in-to-1-in-dia-Plastic-Standard-Pipe-Clamp/1001080726
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¾" diameter (outer tubing diameter) clamps2Secure tubing if needed to coolers/structures4Lowes1414577https://www.lowes.com/pd/HoldRite-10-Pack-3-4-in-to-3-4-in-dia-Plastic-Standard-Pipe-Clamp/1001080692
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Bag filter 100um 7x18"2Filter to attach to float valves to filter incoming water4McMaster Carr2514K18https://www.mcmaster.com/2514K18-2514K11/
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MANIFOLDS
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½" PVC nipple fittings 4"7Manifold for delivery system14Lowes56111https://www.lowes.com/pd/LASCO-1-2-in-x-1-2-in-x-1-2-in-dia-x-4-in-L-Schedule-80-Nipples-PVC-Fitting/3381486
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¾" to ½" barb fitting 1Attach tubing to manifold2Lowes151775https://www.lowes.com/pd/LASCO-3-4-in-x-1-2-in-Dia-Adapter-Plastic-Coil-Fitting/50139800
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½" PVC Tee's (thread-thread-thread)6Manifold outlets 12Lowes317760
https://www.lowes.com/pd/LASCO-1-2-in-x-1-2-in-x-1-2-in-x-1-2-in-dia-Tee-PVC-Fitting/3447452?cm_mmc=shp-_-c-_-prd-_-plb-_-ggl-_-LIA_PLB_142_Pipe-Fittings-_-3447452-_-local-_-0-_-0&ds_rl=1286981&gclid=CjwKCAjw9-KTBhBcEiwAr19ig-ljuKveNWFA5uqwWFzKLfrxOjhxMdiDTvZycaPqb0BKuFYeYIHcIRoCR60QAvD_BwE&gclsrc=aw.ds
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Elbow thread-thread ½" PVC2Attach tubing to manifold4Lowes126788https://www.lowes.com/pd/Charlotte-Pipe-1-2-IN-SCH40-90-DEG-ELBOW/5012475131
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1/4" to 3/16" threaded barbs (part 5218K684)6Attach tubing to manifold and conicals12McMaster Carr5218K684https://www.mcmaster.com/barbed-tube-fittings/for-hose-id~3-16/material~plastic/
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½" to ¼" pvc threaded reducer6Attach tubing barbs to manifold12McMaster Carr4880K343https://www.mcmaster.com/reducers/type~bushing/material~plastic/standard-wall-plastic-pipe-fittings-for-water/
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3/16" silicon tubing (100 ft) 1Tubing to conicals 2Pentairtp30shttps://pentairaes.com/silicone-tubing-3-16.html
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Spring clamps 2-in3Hold PVC manifold to tanks6AmazonWaenlir ‎6.5 inch 6Phttps://www.amazon.com/gp/product/B086DMZ6V6/ref=sw_img_1?smid=AXP93JQNCCGB6&psc=1
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½" PVC threaded caps 1Cap manifolds if needed2Lowes23890https://www.lowes.com/pd/LASCO-1-2-in-x-1-2-in-dia-Cap-PVC-Fitting/3371572
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½" barb threaded fittings1Attach return tubing2Lowes877101https://www.lowes.com/pd/B-K-1-2-in-Barbed-Barb-x-MIP-Adapter-Fitting/1000505043
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½" male - female threaded valve1For optional flow control at end of manifold2McMaster Carr4054N111https://www.mcmaster.com/4054N111/?SrchEntryWebPart_InpBox=tubing+clamp
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Roller tubing clamps (white, plastic) 6Regulating conical flow12WPI Inc14041-60
https://www.wpiinc.com/14041-60-roller-clamp-3-16?gclid=CjwKCAjw0dKXBhBPEiwA2bmObdPwucTLsgDJmV8YZpMY5vKEa-4CGn6s1NITZBD7hGtGHeUrtVsrYBoCVwIQAvD_BwE
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BANJO FILTERS
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4" PVC (will need to be cut into slices) - in 5 ft 0.5Banjo filters 1Lowes23839https://www.lowes.com/pd/Charlotte-Pipe-4-in-x-5-ft-Sch-40-Cellcore-PVC-DWV-Pipe/3133051
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½" PVC (10 ft) - will have extra0.5Banjo filters 1Lowes23966https://www.lowes.com/pd/Charlotte-Pipe-1-2-in-dia-x-10-ft-L-600-PSI-PVC-Pipe/3133079
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½" PVC elbow slip fitting6Banjo filters 12Lowes23867https://www.lowes.com/pd/LASCO-1-2-in-x-1-2-in-x-1-2-in-dia-Slip-Elbow-PVC-Fitting/1067625
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1/2" PVC straight slip fitting6Banjo filters 12Lowes23849https://www.lowes.com/pd/LASCO-1-2-in-x-1-2-in-x-1-2-in-dia-Coupling-PVC-Fitting/3228010
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153um plankton mesh1Mesh for banjo filters (~1 yard per system) 2Wildco3-24-C36https://shop.sciencefirst.com/wildco/nitex-bolting-cloth/6414-nitex-bolting-cloth-nitex-153m.html
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Hot glue and/or sealant 1Secure banjo filters 1Amazon/any supplierAny model/typehttps://www.lowes.com/pd/Gorilla-100-Silicone-All-Purpose-Sealant-10-oz-Clear-Silicone-Caulk/1000217733
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CONICALS
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Food safe containers 6Squaricals (can be approx. 18-22 qt)12Amazon20 or 22 qt Rubbermaid clear https://www.amazon.com/gp/product/B000R8JOWK/ref=ppx_yo_dt_b_asin_title_o01_s00?ie=UTF8&psc=1
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TEMPERATURE CONTROL
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500W heaters3Heaters for header - 2 for cooler, 1 for water bath or extra6Bulk Reef Supply209870https://www.bulkreefsupply.com/finnex-th-deluxe-titanium-heating-element-with-plastic-guard.html
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Apex base control unit1Apex system2Bulk Reef Supply211503https://www.bulkreefsupply.com/apex-controller-base-unit-neptune-systems.html
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Apex EB81Apex system2Bulk Reef Supply210221https://www.bulkreefsupply.com/energybar-832-neptune-systems.html
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Apex PM15Apex system10Bulk Reef Supply207494https://www.bulkreefsupply.com/ph-orp-probe-module-pm1-neptune-systems.html
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Apex temperature probe6Apex temp probes - 1 per squarical12Saltwater AquariumNA
https://www.saltwateraquarium.com/apex-temperature-probe-neptune-systems/?gclid=Cj0KCQjwjbyYBhCdARIsAArC6LLzh-Sbm2wXo_WQcIjP35SbN6wUkp2_AO8ISIw-oNer3eT_RRctvnkaAoufEALw_wcB
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Temperature loggers (pendants)6Temp loggers - 1 per squarical12OnsetUA-002-64
https://www.onsetcomp.com/products/data-loggers/ua-002-64?creative=178258167515&keyword=&matchtype=&network=g&device=c&gclid=Cj0KCQjwjbyYBhCdARIsAArC6LI2osRTm9feKBR_y-QJ2ZbdlVqqHMfHgIVkUAk5X4awg9vf1jTaqfEaAi2VEALw_wcB
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CONSUMABLES/MISC
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Stainless steel tubing clamps 8Secure tubing to each barb and pump16Amazon WINL WL31126https://www.amazon.com/gp/product/B08TW6L32G/ref=sw_img_1?smid=A1FIDH9D8GO0ME&psc=1
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Teflon tape2Sealing all manifold connections4Lowes456833https://www.lowes.com/pd/Oatey-0-6-in-x-43-ft-Plumber-s-Tape/4751313
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Stainless Steel Nuts 6Attach to temperature loggers if needed 12Lowes817718https://www.lowes.com/pd/Hillman-1-2-in-x-13-Stainless-Steel-Hex-Nut/1000213001
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Zip ties (assorted sizes) 1General use1Lowes 875390https://www.lowes.com/pd/Utilitech-500-Pack-Cable-Ties/1000865854
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Screws stainless steel exterior 1General use, secure tubing clamps1Lowes3710817https://www.lowes.com/pd/Simpson-Strong-Tie-6-x-1-2-in-Pan-Head-Stainless-Steel-Marine-Screw-25-Qty/5001609125
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Hole saw1To drill holes in squaricals/banjos - need ½-1" in set 1Lowes 1061480https://www.lowes.com/pd/CRAFTSMAN-7-Piece-Bi-Metal-Non-Arbored-Hole-Saw-Set/1000594041
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Sand paper1Sand squaricals and banjos after hole saw1Lowes788454https://www.lowes.com/pd/3M-22-Pack-9-in-W-x-11-in-L-Multi-grade-Pack-Grit-Commercial-Sandpaper/1000065531
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Drill1Drill if wanted, need at least a screwdriver for in field use1LowesExample, any drill OKhttps://www.lowes.com/pd/BLACK-DECKER-20-Volt-Max-3-8-in-Cordless-Drill-Charger-Included/1000036981
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