20170406_qPCR_WSN1_capstone

	A	B	C	D	E	F
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5	Plate #1 master mix
6		Ci	Cf	Vol. (uL)	# Rxns	Total Vol (uL)
7
8	Template			2
9	Promega 2x GoTaq Probe Master Mix	2	1	12.5	106	1325
10	WSN1 F	10	0.32	0.8	106	84.8
11	WSN1 R	10	0.32	0.8	106	84.8
12	RLP_P (biosearch)	10	0.2	0.5	106	53
13	BSA	20	0.60	0.75	106	79.5
14	H2O			7.65	106	810.9
15	Total			25		Add 23uL to each well
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19	Plate #2 master mix
20		Ci	Cf	Vol. (uL)	# Rxns	Total Vol (uL)
21
22	Template			2
23	Promega 2x GoTaq Probe Master Mix	2	1	12.5	60	750
24	WSN1 F	10	0.32	0.8	60	48
25	WSN1 R	10	0.32	0.8	60	48
26	RLP_P (biosearch)	10	0.2	0.5	60	30
27	BSA	20	0.60	0.75	60	45
28	H2O			7.65	60	459
29	Total			25		Add 23uL to each well
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