UMGC Sequencer Home - Google Drive


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2	Illumina NGS Project Home						Questions? E-mail: next-gen@umn.edu. This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
3	This "Dashboard" page serves as a simplifed version of the UMGC Sequencer Home Sample and Run Queues. See the explainer at right for more details.						This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
4	Notice: All sequencing queues are operating normally.
5	Project	Sample Queue	Run Type	Est'd Lib Completion	Est'd Run Completion	Project Status
6	10X_Nano_Pool_138	MiSeq Standard	2x150 Micro Run (4M Reads)	7/6/2022		Library Prep
7	10X_Nano_Pool_139	MiSeq Standard	2x150 Nano Run (1M Reads)	7/22/2022		Library Prep
8	10X_Nano_Pool_140	MiSeq Standard	2x150 Nano Run (1M Reads)	7/26/2022		Library Prep
9	Baughn_029/030	NovaSeq 2x50 Paired-end	2x50 Paired-end	7/8/2022		Library Prep
10	Boeve_004	Stowaway-Seq	2x300 Paired-end	6/8/2022	6/12/2022	Post Run QC
11	Borthakur_002	Stowaway-Seq	2x300 Paired-end	7/6/2022		Library Prep
12	Charter_Service_029	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/24/2022	7/3/2022	Post Run QC
13	Costa5_002	Stowaway-Seq	2x300 Paired-end	6/21/2022	6/19/2022	Post Run QC
14	Dehm_Project_079	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/29/2022		Library Prep
15	Ebner2_003	Stowaway-Seq	2x300 Paired-end	6/10/2022	6/12/2022	Post Run QC
16	Falcao-Salles_013_ReSeq	Stowaway-Seq	2x300 Paired-end	6/8/2022	6/12/2022	Post Run QC
17	Garry_108	NovaSeq 2x50 Paired-end	2x50 Paired-end	6/1/2022		In Seq Queue
18	GBS_Group_006	NovaSeq 2x250 Paired-end	2x250 Paired-end	6/30/2022		Library Prep
19	GBS_Group_008	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/30/2022		Library Prep
20	GBS_Group_011	NextSeq Standard	P2 1x100 (360M Reads)	6/30/2022		Library Prep
21	GBS_Group_018	NovaSeq 2x150 Paired-end	2x150 Paired-end	7/27/2022		Library Prep
22	Goldstrohm_030	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/29/2022	7/13/2022	In Seq Queue
23	Gomez_031	Stowaway-Seq	2x300 Paired-end	7/6/2022		Library Prep
24	Hamilton2_032	Stowaway-Seq	2x300 Paired-end	7/6/2022		Library Prep
25	HealthPartners_Project_007	MiSeq Expedited	2x300 Version 3 Chemistry (16-22M Reads)	7/1/2022	7/3/2022	Sequencing
26	Kalan_011	Stowaway-Seq	2x300 Paired-end	7/6/2022		Library Prep
27	Kashyap_017_Batch6_NanoQC	MiSeq Standard	2x150 Nano Run (1M Reads)	7/18/2022		Library Prep
28	Kashyap_017_Batch_6	NovaSeq 2x150 Paired-end	2x150 Paired-end	7/25/2022		Library Prep
29	Kelekar_006	NovaSeq 2x50 Paired-end	2x50 Paired-end	7/22/2022		Library Prep
30	Kimball_Project_009	NovaSeq 2x150 Paired-end	2x150 Paired-end	5/1/2022	7/3/2022	Post Run QC
31	Kinkel_050_Run_1	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
32	Kinkel_050_Run_6	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
33	Kinkel_050_Run_7	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
34	Kinkel_050_Run_8	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
35	Kinkel_050_Run_9	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
36	Klatt_038_Pilot	MiSeq Standard	2x150 Nano Run (1M Reads)	7/5/2022		Library Prep
37	Klatt_045	Stowaway-Seq	2x300 Paired-end	6/21/2022	6/19/2022	Post Run QC
38	Lillo_011	Stowaway-Seq	2x300 Paired-end	6/21/2022	6/19/2022	Post Run QC
39	Lin2_006	Stowaway-Seq	2x300 Paired-end	7/5/2022		Library Prep
40	Marchan-Rivandeneira_002	Stowaway-Seq	2x300 Paired-end	6/17/2022		In Seq Queue
41	Marks_063	NovaSeq 2x150 Paired-end	2x150 Paired-end	7/13/2022		Library Prep
42	May_020	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	6/28/2022		Library Prep
43	McGaugh2_Project_024_Pool_1-2	NovaSeq 1x100 Single Read	1x100 Single Read	6/27/2022	6/30/2022	Post Run QC
44	McGaugh2_Project_024_Pool_3-4	NovaSeq 1x100 Single Read	1x100 Single Read	7/12/2022		Library Prep
45	McGaugh2_Project_024_Pool_5-6	NovaSeq 1x100 Single Read	1x100 Single Read	7/12/2022		Library Prep
46	Modiano_Project_060	NovaSeq 2x100 Paired-end	2x100 Paired-end	7/1/2022		Library Prep
47	Modiano_Project_060	NextSeq Standard
48	Modiano_Project_060	NextSeq Standard
49	Mor_282	MiSeq VDL	2x300 Version 3 Chemistry (16-22M Reads)	6/30/2022	7/3/2022	Post Run QC
50	Offer_010	NovaSeq 2x250 Paired-end	2x250 Paired-end	7/13/2022		Library Prep
51	Otto_007	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/17/2022		In Seq Queue
52	Palloni_001_Run_2	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
53	Piccini_002/Hu2_005	Stowaway-Seq	2x300 Paired-end	6/21/2022	6/19/2022	Post Run QC
54	Ponder_Project_013	Stowaway-Seq	2x300 Paired-end	6/24/2022		In Seq Queue
55	Povero_Project_003	NovaSeq 2x100 Paired-end	2x100 Paired-end	7/1/2022		Library Prep
56	Povero_Project_003	NextSeq Standard
57	Rebollar_005_Run_1	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
58	Rebollar_005_Run_3	MiSeq Standard	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep
59	Seibold_018	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/24/2022	7/13/2022	In Seq Queue
60	Seibold_019	NovaSeq 2x150 Paired-end	2x150 Paired-end	7/14/2022		Library Prep
61	Singh4_001	NovaSeq 2x150 Paired-end	2x150 Paired-end			Delayed
62	Singh6_001	NextSeq Standard	P1 2x150 (90M Reads)	6/28/2022	6/29/2022	Post Run QC
63	Singh6_Project_002	MiSeq Standard	2x75 Version 3 Chemistry (16-22M Reads)	6/9/2022	6/10/2022	Post Run QC
64	Skinner_019	NovaSeq 2x50 Paired-end	2x50 Paired-end			Delayed
65	Skinner_022	NovaSeq 2x50 Paired-end	2x50 Paired-end	7/15/2022		Library Prep
66	Skinner_023	NextSeq Standard	P1 2x150 (90M Reads)	7/15/2022		#REF!
67	Staley_079	Stowaway-Seq	2x300 Paired-end	5/4/2022	6/12/2022	Post Run QC
68	Staley_080	Stowaway-Seq	2x300 Paired-end	5/4/2022	6/12/2022	Post Run QC
69	Staley_081	Stowaway-Seq	2x300 Paired-end	7/5/2022		Library Prep
70	Staley_082	Stowaway-Seq	2x300 Paired-end	7/6/2022		Library Prep
71	Starr_155	Stowaway-Seq	2x300 Paired-end	6/1/2022	6/12/2022	Post Run QC
72	Starr_156	Stowaway-Seq	2x300 Paired-end	6/1/2022	6/12/2022	Post Run QC
73	Steinacker_002	Stowaway-Seq	2x300 Paired-end	7/6/2022		Library Prep
74	Stupar_054	NovaSeq 2x150 Paired-end	2x150 Paired-end	7/15/2022		Library Prep
75	Subramanian_052	Stowaway-Seq	2x300 Paired-end	6/8/2022	6/12/2022	Post Run QC
76	Subramanian_052_ReSeq	Stowaway-Seq	2x300 Paired-end	6/21/2022		Post Run QC
77	Thum_009	NovaSeq 2x150 Paired-end	2x150 Paired-end	7/8/2022	7/13/2022	In Seq Queue
78	Tsai_075_Pool_2	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/23/2022	7/13/2022	In Seq Queue
79	Tsai_075_Pool_3	NovaSeq 2x150 Paired-end	2x150 Paired-end	6/29/2022		Library Prep
80	UMGC_IL_065	MiSeq Standard	2x150 Nano Run (1M Reads)	7/8/2022		Library Prep
81	Webber_009	NovaSeq 2x150 Paired-end	2x150 Paired-end	7/18/2022		Library Prep
82	Webber_009_NanoQC	MiSeq Standard	2x150 Nano Run (1M Reads)	7/18/2022		Library Prep
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1
2	NovaSeq Run Calendar			Questions? E-mail: next-gen@umn.edu
3
4	Flow cell position: A	Flow cell position: B		This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
5	Idle	Idle
6
7	Current Run	Current Run		Explainer: The tables on the left report on current and queued runs in the two flow cell positions (A and B) on the Illumina NovaSeq 6000 Sequencer. The sequencer can run two flow cells at a time. SP, S1 and S2 flow cells have 2 lanes, while S4 flow cells have 4. One lane generates between 375M and 2,250M reads, depending on which flow cell is used (SP, S1, S2 or S4) resulting in the lowest-cost NGS data available. To view the queue of samples that are currently being prepared for sequencing on Nova, click on the NovaSeq Sample Queue tab above.
8	Start	Start
9	End	End
10	Elapsed	Elapsed
11	Run Type	Run Type
12	Run ID	Run ID
13	Lane 1	Lane 1
14	Lane 2	Lane 2
15	Lane 3	Lane 3
16	Lane 4	Lane 4
17
18	Queued Run A1	Queued Run B1		Queued Run Notes: Note 1: For queued runs, the Est'd Start date will be entered once all lanes are filled, or at such time that we decide to sequence the flow cell despite the fact that it is only partially filled. Note 2: Clients may be able to accelerate the running of a partially-filled flow cell by requesting an expansion of their sequencing to fill the empty lanes. To discuss adding additional lanes to a queued flow cell, E-mail: next-gen@umn.edu. Note 3: Libraries shown in yellow are not yet on hand in the sequencer suite, but are expected imminently, and have therefore been mapped to a run. Note 4: Libraries shown in purple are CLIA libraries
19	Est'd Start	Est'd Start	7/11/2022
20	Est'd End	Est'd End	7/13/2022
21	Run Type	Run Type	S4 2x150-bp Paired-End DI
22	Lane 1	Lane 1	Goldstrohm_030
23	Lane 2	Lane 2	Seibold_018
24	Lane 3	Lane 3	Thum_009
25	Lane 4	Lane 4	Tsai_075_Pool_2
26
27	Queued Run A2	Queued Run B2
28	Est'd Start	Est'd Start
29	Est'd End	Est'd End
30	Run Type	Run Type
31	Lane 1	Lane 1
32	Lane 2	Lane 2
33	Lane 3	Lane 3
34	Lane 4	Lane 4
35
36	Queued Run A3	Queued Run B3
37	Est'd Start	Est'd Start
38	Est'd End	Est'd End
39	Run Type	Run Type
40	Lane 1	Lane 1
41	Lane 2	Lane 2
42	Lane 3	Lane 3
43	Lane 4	Lane 4
44
45	Queued Run A4	Queued Run B4
46	Est'd Start	Est'd Start
47	Est'd End	Est'd End
48	Run Type	Run Type
49	Lane 1	Lane 1
50	Lane 2	Lane 2
51	Lane 3	Lane 3
52	Lane 4	Lane 4


1
2	NovaSeq Sample Queue (Projects sequencing, or in queue for sequencing).																						This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
3
4	2x150-bp Paired-end											2x50-bp Paired-end
5	Project	# of Lanes	Flow Cell Type	Loading Protocol	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Run Queue Position	# Lanes Filled	Est'd Run Date	Est'd Run Completion	Project	# of Lanes	Flow Cell Type	Loading Protocol	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Run Queue Position	# Lanes Filled	Est'd Run Date	Est'd Run Completion	Explainer:
6	Tsai_075_Pool_2	1	S4	XP	6/23/2022	6/23/2022	In Seq Queue	B1	4/4	7/11/2022	7/13/2022	Skinner_019	1	SP	XP			Delayed	Unassigned				The tables on the left report currently sequencing -- and future -- NovaSeq sequencing libraries. We are currently only selling NovaSeq runs as single lanes or full flow cells. Fractional lanes are not allowed except for special UMGC internal service runs The availability of libraries determines the timing of queued NovaSeq runs, the planning of which is shown. The exact make-up of queued runs is liable to change depending on the timing of completion of libraries. PROJECT: the UMGC unique project ID, in format Investigator_### # OF LANES: the number of NovaSeq lanes requested EST'D LIB COMPLETION: the date when libraries are expected to complete library preparation and final QC. These include both UMGC-created and client-created libraries, and the estimated date may change due to a number of factors. DATE LIBRARIES R'CVD IN CCRB: the date on which the libraries were received in CCRB and made available for sequencing. Field turns yellow when date is 2 weeks past receipt and red when 3 weeks past receipt. If 3 weeks past and sequencing run has not launched, PI and Submitter will receive alternate options for sequencing via email. PROJECT STATUS: The current status of the libraries for the project. Status is described in detail on ILLUMINA NGS PROJECT HOME page. To view current sequencing run queue, see SEQUENCER RUNS tab for each platform at top of this page. # LANES FILLED: the number of lanes (out of 2 or 4 depending on flow cell type) that have been filled for queued runs. Green shading indicates a filled flow cell, whereas red shading indicates that there are too few libraries accumulated to permit the run to proceed. ESTIMATED RUN DATE: the date on which queued runs are estimated to be launched. ESTIMATED RUN COMPLETION: the date on which queued runs are estimated to complete. Data delivery is generally within 1-4 business days after completion.
7	Seibold_018	1	S4	XP	6/24/2022	6/22/2022	In Seq Queue	B1	4/4	7/11/2022	7/13/2022	Garry_108	1	S1	XP	6/1/2022	6/2/2022	In Seq Queue	Unassigned	1/2
8	Goldstrohm_030	1	S4	XP	6/29/2022	6/29/2022	In Seq Queue	B1	4/4	7/11/2022	7/13/2022	Kelekar_006	1	S1	XP	7/22/2022		Library Prep	Unassigned
9	Thum_009	1	S4	XP	7/8/2022	6/30/2022	In Seq Queue	B1	4/4	7/11/2022	7/13/2022	Baughn_029/030	1	SP	XP	7/8/2022		Library Prep	Unassigned
10	Kimball_Project_009	1	S4	XP	5/1/2022	6/21/2022	Post Run QC	History	4/4	7/1/2022	7/3/2022	Skinner_022	1	SP	XP	7/15/2022		Library Prep	Unassigned
11	Charter_Service_029	2	S4	XP	6/24/2022	6/28/2022	Post Run QC	History	4/4	7/1/2022	7/3/2022
12	Singh4_001	3	S4	XP			Delayed	Unassigned
13	Otto_007	1	SP	XP	6/17/2022	6/20/2022	In Seq Queue	Unassigned	1/2
14	GBS_Group_008	1	S1	XP	6/30/2022		Library Prep	Unassigned
15	Webber_009	2	S1	Standard	7/18/2022		Library Prep	Unassigned
16	Tsai_075_Pool_3	1	S4	XP	6/29/2022		Library Prep	Unassigned
17	Dehm_Project_079	1	S4	XP	6/29/2022		Library Prep	Unassigned
18	Marks_063	2	S4	XP	7/13/2022		Library Prep	Unassigned
19	Seibold_019	1	S4	XP	7/14/2022		Library Prep	Unassigned
20	Stupar_054	2	S4	XP	7/15/2022		Library Prep	Unassigned
21	Kashyap_017_Batch_6	1	S4	XP	7/25/2022		Library Prep	Unassigned
22	GBS_Group_018	2	S4	XP	7/27/2022		Library Prep	Unassigned
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43	2x100-bp Paired-end											1x100-bp Single Read
44	Project	# of Lanes	Flow Cell Type	Loading Protocol	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Run Queue Position	# Lanes Filled	Est'd Run Date	Est'd Run Completion	Project	# of Lanes	Flow Cell Type	Loading Protocol	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Run Queue Position	# Lanes Filled	Est'd Run Date	Est'd Run Completion
45	Povero_Project_003	1	SP	XP	7/1/2022		Library Prep	Unassigned				McGaugh2_Project_024_Pool_1-2	2	S1	XP	6/27/2022	6/28/2022	Post Run QC	History	2/2	6/29/2022	6/30/2022
46	Modiano_Project_060	1	SP	XP	7/1/2022		Library Prep	Unassigned				McGaugh2_Project_024_Pool_3-4	2	S1	XP	7/12/2022		Library Prep	Unassigned
47												McGaugh2_Project_024_Pool_5-6	2	S1	XP	7/12/2022		Library Prep	Unassigned
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67	2x250-bp Paired-end											Reserved For Future Run Configuration
68	Project	# of Lanes	Flow Cell Type	Loading Protocol	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Run Queue Position	# Lanes Filled	Est'd Run Date	Est'd Run Completion	Project	# of Lanes	Flow Cell Type	Loading Protocol	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Run Queue Position	# Lanes Filled	Est'd Run Date	Est'd Run Completion
69	GBS_Group_006	2	SP	Standard	6/30/2022		Library Prep	Unassigned
70	Offer_010	2	SP	Standard	7/13/2022		Library Prep	Unassigned
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1
2		NextSeq 2000	Questions? E-mail: next-gen@umn.edu
3		Idle	This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
4
5	Current Run	Start
6		End
7		Read Length	Explainer: The table on the left reports current and queued runs on the NextSeq 2000. The NextSeq can run one flow cell at a time in various flexible length configurations and outputs (High-output P3, Mid-output P2 paired-end or single read for the 100 cycle kit). Each flow cell will be assigned a single client project except in the case of GBS pilot runs. These runs are assigned as shared runs by UMGC staff. NextSeq 2K runs have a duration of 1 to 2 days and are ideal for small to mid-output NGS projects. Expedited runs are available on the platform for an expedited fee. To view the queue of samples that are currently being prepared for sequencing, click on the NextSeq Sample Queue tab above.
8		Queue Type
9		Projects
10
11	Queued Run #1	Start
12		End
13		Read Length
14		Queue Type
15		Projects
16
17	Queued Run #2	Start
18		End
19		Read Length
20		Queue Type
21		Projects
22
23	Queued Run #3	Start
24		End
25		Read Length
26		Queue Type
27		Projects


1
2	NextSeq 2000 Standard Sample Queue									This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
3
4	Project	Run Type	# of Lanes	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Queue Position	Est'd Run Date	Est'd Run Completion	Explainer:
5	Singh6_001	P1 2x150 (90M Reads)	1	6/28/2022	6/24/2022	Post Run QC	History	6/28/2022	6/29/2022	The tables on the left report currently sequencing -- and future -- NextSeq sequencing libraries. We are currently only selling NextSeq runs as full flow cells (i.e. no shared runs or fractional runs). The availability of libraries determines the timing of queued NextSeq runs, the planning of which is shown. PROJECT: the UMGC unique project ID, in format Investigator_### # OF LANES: currently always 1. EST'D LIB COMPLETION: the date when libraries are expected to complete library preparation and final QC. These include both UMGC-created and client-created libraries, and the estimated date may change due to a number of factors. DATE LIBRARIES R'CVD IN CCRB: the date on which the libraries were received in CCRB and made available for sequencing. Field turns yellow when date is 2 weeks past receipt and red when 3 weeks past receipt. If 3 weeks past and sequencing run has not launched, PI and Submitter will receive alternate options for sequencing via email. PROJECT STATUS: The current status of the libraries for the project. Status is described in detail on ILLUMINA NGS PROJECT HOME page. To view current sequencing run queue, see SEQUENCER RUNS tab for each platform at top of this page. ESTIMATED RUN DATE: the date on which queued runs are estimated to be launched. ESTIMATED RUN COMPLETION: the date on which queued runs are estimated to complete. Data delivery is generally within 2-4 business days after completion.
6	GBS_Group_011	P2 1x100 (360M Reads)	1	6/30/2022		Library Prep	Unassigned
7	Skinner_023	P1 2x150 (90M Reads)	1	7/15/2022		Library Prep	Unassigned
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30		# Future Lanes	3


1
2		MiSeq #1 (Standard Sample Queue)	MiSeq #2 (Standard Sample Queue)	MiSeq #3 (Priority: Expedited Runs)		MiSeq #4 (VDL MiSeq) (Priority: VDL)	MiSeq #5 (Standard Sample Queue)
3		Idle	Under Repair	Running		Idle	Idle
4
5	Current Run	Start	Start	Start	7/1/2022	Start	Start
6		End	End	End	7/3/2022	End	End
7		Read Length	Read Length	Read Length	2x300 Version 3 Chemistry (16-22M Reads)	Read Length	Read Length
8		Queue Type	Queue Type	Queue Type	Expedited Sample Queue	Queue Type	Queue Type
9		Projects	Projects	Projects	HealthPartners_Project_007	Projects	Projects
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11	Queued Run #1	Start	Start	Start		Start	Start
12		End	End	End		End	End
13		Read Length	Read Length	Read Length		Read Length	Read Length
14		Queue Type	Queue Type	Queue Type		Queue Type	Queue Type
15		Projects	Projects	Projects		Projects	Projects
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17	Queued Run #2	Start	Start	Start		Start	Start
18		End	End	End		End	End
19		Read Length	Read Length	Read Length		Read Length	Read Length
20		Queue Type	Queue Type	Queue Type		Queue Type	Queue Type
21		Projects	Projects	Projects		Projects	Projects
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23	Queued Run #3	Start	Start	Start		Start	Start
24		End	End	End		End	End
25		Read Length	Read Length	Read Length		Read Length	Read Length
26		Queue Type	Queue Type	Queue Type		Queue Type	Queue Type
27		Projects	Projects	Projects		Projects	Projects
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29	Queued Run #4	Start	Start	Start		Start	Start
30		End	End	End		End	End
31		Read Length	Read Length	Read Length		Read Length	Read Length
32		Queue Type	Queue Type	Queue Type		Queue Type	Queue Type
33		Projects	Projects	Projects		Projects	Projects


2
3	Standard Libraries (First-come, First-served, MiSeqs #1-#3, #5) Standard runs are ordinarily scheduled on MiSeq #1 or #2, but may be run on MiSeqs #3-#5 if those instruments are open.									Veterinary Diagnostic Lab (VDL) Libraries (MiSeq #4) VDL samples receive priority on MiSeq #4, which is owned by the VDL and operated by the UMGC. MiSeq #4 is used for "Standard" runs only when the VDL does not have libraries ready for sequencing.										Expedited Libraries For clients who need more rapid turnaround, MiSeq #3 -- un unsubsidized instrument -- is reserved for their use. Although runs on MiSeq #3 are about 50% more expensive than on MiSeq #1-#2 & #4-#5, there is typically no queue to get on the instrument. MiSeq #3 is used for "Standard" runs only when there are no expedited libraries ready for sequencing.
4
5	Project	Run Type	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Queue Position	Est'd Run Date	Est'd Run Completion	MiSeq #	Project	Run Type	Est'd Lib Completion	Date Libraries R'cvd in Snyder	Priority	Project Status	Queue Position	Est'd Run Date	Est'd Run Completion	MiSeq #	Project	Run Type	# of Lanes	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Queue Position	Est'd Run Date	Est'd Run Completion	MiSeq #
6	Singh6_Project_002	2x75 Version 3 Chemistry (16-22M Reads)	6/9/2022	6/9/2022	Post Run QC	History	6/9/2022	6/10/2022	MiSeq #3	Mor_282	2x300 Version 3 Chemistry (16-22M Reads)	6/30/2022	6/30/2022	Priority 1	Post Run QC	History	7/1/2022	7/3/2022	MiSeq #1	HealthPartners_Project_007	2x300 Version 3 Chemistry (16-22M Reads)	1	7/1/2022	7/1/2022	Sequencing	1. Running	7/1/2022	7/3/2022	MiSeq #3
7	May_020	2x300 Version 3 Chemistry (16-22M Reads)	6/28/2022		Library Prep	Unassigned
8	Klatt_038_Pilot	2x150 Nano Run (1M Reads)	7/5/2022		Library Prep	Unassigned
9	10X_Nano_Pool_138	2x150 Micro Run (4M Reads)	7/6/2022		Library Prep	Unassigned
10	UMGC_IL_065	2x150 Nano Run (1M Reads)	7/8/2022		Library Prep	Unassigned
11	Palloni_001_Run_1	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
12	Palloni_001_Run_2	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
13	Rebollar_005_Run_1	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
14	Rebollar_005_Run_2	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
15	Rebollar_005_Run_3	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
16	Kinkel_050_Run_1	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
17	Kinkel_050_Run_2	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
18	Kinkel_050_Run_3	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
19	Kinkel_050_Run_4	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
20	Kinkel_050_Run_5	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
21	Kinkel_050_Run_6	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
22	Kinkel_050_Run_7	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
23	Kinkel_050_Run_8	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
24	Kinkel_050_Run_9	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
25	Kinkel_050_Run_10	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
26	Kinkel_050_Run_11	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
27	Kinkel_050_Run_12	2x300 Version 3 Chemistry (16-22M Reads)	7/8/2022		Library Prep	Unassigned
28	Marks_063_NanoQC	2x150 Nano Run (1M Reads)	7/11/2022		Library Prep	Unassigned
29	Stupar_054_NanoQC	2x150 Nano Run (1M Reads)	7/11/2022		Library Prep	Unassigned
30	Kashyap_017_Batch6_NanoQC	2x150 Nano Run (1M Reads)	7/18/2022		Library Prep	Unassigned
31	Webber_009_NanoQC	2x150 Nano Run (1M Reads)	7/18/2022		Library Prep	Unassigned
32	10X_Nano_Pool_139	2x150 Nano Run (1M Reads)	7/22/2022		Library Prep	Unassigned
33	10X_Nano_Pool_140	2x150 Nano Run (1M Reads)	7/26/2022		Library Prep	Unassigned
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1
2		iSeq #1 (Standard Sample Queue)	Questions? E-mail: next-gen@umn.edu
3		Idle	This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
4
5	Current Run	Start
6		End
7		Read Length	Explainer: The table on the left reports current and queued runs on the iSeq. The iSeq can run one flow cell at a time in a single 2x151-bp configuration. A 2x250-bp kit is expected to be available soon. iSeq runs have a duration of approximately 20 hours and are ideal for amplicon sequencing, targeted NGS applications and other low output NGS projects. Expedited runs are available on the platform for an expedited fee. To view the queue of samples that are currently being prepared for sequencing, click on the iSeq Sample Queue tab above.
8		Queue Type
9		Projects
10
11	Queued Run #1	Start
12		End
13		Read Length
14		Queue Type
15		Projects
16
17	Queued Run #2	Start
18		End
19		Read Length
20		Queue Type
21		Projects
22
23	Queued Run #3	Start
24		End
25		Read Length
26		Queue Type
27		Projects


1
2	iSeq Standard Libraries									iSeq Expedited Libraries For clients who need more rapid turnaround, this queue can be accessed for an additional fee.									This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
3
4	Project	Run Type	# of Lanes	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Queue Position	Est'd Run Date	Est'd Run Completion	Project	Run Type	# of Lanes	Est'd Lib Completion	Date Libraries R'cvd in CCRB	Project Status	Queue Position	Est'd Run Date	Est'd Run Completion	Explainer:
5																			The tables on the left report currently sequencing -- and future -- iSeq sequencing libraries. We are currently only selling NextSeq runs as full flow cells (i.e. no shared runs or fractional runs). The availability of libraries determines the timing of queued iSeq runs, the planning of which is shown. PROJECT: the UMGC unique project ID, in format Investigator_### # OF LANES: currently always 1. EST'D LIB COMPLETION: the date when libraries are expected to complete library preparation and final QC. These include both UMGC-created and client-created libraries, and the estimated date may change due to a number of factors. DATE LIBRARIES R'CVD IN CCRB: the date on which the libraries were received in CCRB and made available for sequencing. Field turns yellow when date is 2 weeks past receipt and red when 3 weeks past receipt. If 3 weeks past and sequencing run has not launched, PI and Submitter will receive alternate options for sequencing via email. PROJECT STATUS: The current status of the libraries for the project. Status is described in detail on ILLUMINA NGS PROJECT HOME page. To view current sequencing run queue, see SEQUENCER RUNS tab for each platform at top of this page. ESTIMATED RUN DATE: the date on which queued runs are estimated to be launched. ESTIMATED RUN COMPLETION: the date on which queued runs are estimated to complete. Data delivery is generally within 1-3 business days after completion.
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26		# Future Lanes	0								# Future Lanes	0


1
2	Stowaway-Seq Run Calendar.
3	Stowaway-Seq (2x300 PE MiSeq Runs) Stowaway-Seq is a service designed to provide shallow (≤ 2M reads), low-cost ($322.14) sequencing for small-scale pilot experiments. We achieve this by a) pooling projects from many different users, b) managing the addition of indexing barcodes to ensure that pooled libraries are compatible, and c) running all projects using a read length (2x300-bp PE) that encompasses any desired sequencing flavor. Please read FAQs below for more information, contact Corbin Dirkx (micbiom@umn.edu, 16S samples or amplicons) or Aaron Becker (next-gen@umn.edu, other library types).
4
5	Run Date and Status			Submission Cutoff Dates For Client-made Libraries		Submission Cutoff Dates For Amplicons or 16S Samples (Important: See FAQ #1!)		Run Details
6	Scheduled Run Date	Days Until Run	Status	Cutoff Date	Days Remaining Until Cutoff Date	Cutoff Date	Days Remaining Until Cutoff Date	Project	Project Status	# of 1/8th Lanes Requested	Est'd Lib Completion	Date Libraries received in CCRB	Est'd Run Completion	MiSeq #
7	Thursday, 06-09-22	Will launch in -25 days.	Completed	Thursday, 05-26-22	Cutoff date is past. This Stowaway-Seq run is closed.	Thursday, 05-12-22	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Ebner2_003	Post Run QC	1	6/10/2022	6/10/2022	6/12/2022	MiSeq #5
8								Falcao-Salles_013_ReSeq	Post Run QC	1	6/8/2022	6/8/2022	6/12/2022
9								Staley_079	Post Run QC	1	5/4/2022	5/4/2022	6/12/2022
10								Staley_080	Post Run QC	1	5/4/2022	5/4/2022	6/12/2022
11								Starr_155	Post Run QC	1	6/1/2022	6/8/2022	6/12/2022
12								Starr_156	Post Run QC	1	6/1/2022	6/8/2022	6/12/2022
13								Subramanian_052	Post Run QC	1	6/8/2022	6/8/2022	6/12/2022
14								Boeve_004	Post Run QC	1	6/8/2022	6/8/2022	6/12/2022
15	Tuesday, 06-21-22	Will launch in -13 days.	Completed	Tuesday, 06-07-22	Cutoff date is past. This Stowaway-Seq run is closed.	Friday, 05-27-22	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Costa5_002	Post Run QC	1	6/21/2022	6/17/2022	6/19/2022	MiSeq #3
16								Piccini_002/Hu2_005	Post Run QC	1	6/21/2022	6/17/2022	6/19/2022
17								Klatt_045	Post Run QC	2	6/21/2022	6/17/2022	6/19/2022
18								Lillo_011	Post Run QC	4	6/21/2022	6/17/2022	6/19/2022
19								Subramanian_052_ReSeq	Post Run QC	1	6/21/2022	6/17/2022	6/19/2022
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23	Wednesday, 07-06-22	Will launch in 2 days.	Next Up	Wednesday, 06-15-22	Cutoff date is past. This Stowaway-Seq run is closed.	Wednesday, 06-08-22	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Hamilton2_032	Library Prep	2	7/6/2022
24								Borthakur_002	Library Prep	1	7/6/2022
25								Staley_082	Library Prep	2	7/6/2022
26								Steinacker_002	Library Prep	1	7/6/2022
27								Kalan_011	Library Prep	1	7/6/2022
28								Gomez_031	Library Prep	2	7/6/2022
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31	Friday, 07-22-22	Will launch in 18 days.		Friday, 07-08-22	You have 4 days remaining to submit client-made libraries.	Friday, 06-24-22	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Ponder_Project_013	In Seq Queue	3	6/24/2022	6/24/2022
32								Marchan-Rivandeneira_002	In Seq Queue	1	6/17/2022	6/24/2022
33								Lin2_006	Library Prep	2	7/5/2022
34								Staley_081	Library Prep	1	7/5/2022
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39	Friday, 08-12-22	Will launch in 39 days.		Friday, 07-29-22	You have 25 days remaining to submit client-made libraries.	Friday, 07-15-22	You have 11 days remaining to submit amplicons or 16S samples.
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47
48	FAQ:
49	1) How much space can I request?
50	You may request increments of 1/8th of a MiSeq Lane to a maximum of 4 "lanes", if space allows. UMGC staff reserves the right to limit lane requests to a single "lane" per run, per user when high request volumes occur. If a large number of submissions are received, we will create multiple stowaway runs for a given submission period.
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52	2) What are the charges?
53	UMGC will charge users in increments of 1/8th of a lane ($322.14 per 1/8 th of a lane) requested.
54
55	3) What samples are acceptable?
56	Libraries that have been indexed and pooled will be accepted two weeks before the Stowaway-Seq run date. We may accept samples for library creation for Stowaway service (e.g., amplicons or 16S samples), but ONLY when prior approval has been obtained, with enough time for the library creation to occur.
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58	4) Do you perform QC on libraries?
59	Yes. We perform Kapa QC on samples to ensure functional library.
60
61	5) What is the deadline for submission?
62	For client-made libraries, the cut-off for submission is at least 14 days prior to the scheduled run. For amplicons and 16S samples, the cut-off for submission is at least 28 days prior to the scheduled run.
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64	6) Can I request Expedited Service for a Stowaway run?
65	No. The purpose of Stowaway-Seq is not speed, but low cost. The runs are scheduled in advance and guaranteed to run on the scheduled date, in order to provide a reliable, low-cost option for all.
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67	7) How do I request this service?
68	Please refer to this Google Doc to see the availibility of the next run.
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70	8) How can I know what barcodes to use for my samples – so that they do not overlap with another researcher’s barcodes?
71	Please email next-gen@umn.edu with your intended barcodes. We will check it against others currently in the run and notify you.
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73	9) What if I miss the deadline for submission?
74	UMGC will try to accommodate your request on the next scheduled run provided there is no barcode conflict.
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76	10) This is great, but I can't seem to get on a Stowaway-Seq run because they fill up too fast, and my submission type is of low priority according to your policies!!!
77	We have the ability to increase the frequency of Stowaway-Seq runs, and will do so if we find that demand justifies it.
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79	11) I still have questions. Who can I contact?
80	Please contact the Aaron Becker (Illumina Sequencing Services Manager) at becke044@umn.edu for questions regarding client-made library submission, or Corbin Dirkx (High-throughput Sequencing Services Manager) at micbiom@umn.edu for questions regarding amplicon or 16S sample submission.


1
2	Charter Service RNA-Seq Calendar
3	Charter Service RNA-Seq (2x150 PE NovaSeq Runs) Charter Service is the UMN Genomics Center's new RNA-Seq service at $179/sample (UMN rate, library prep + sequencing) that provides a monthly shared NovaSeq run that departs on the 1st business day of the month with data arriving the 1st business day of the following month. Carry-on luggage is restricted to 20 million reads/sample on a 150 PE run, but additional space can be purchased in 20-million-read increments at $100/sample. More details at http://genomics.umn.edu/ngs-library-rna.php
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5	Departure Date and Status			Cutoff Dates For Seat Reservation		Flight Arrival (Data Delivery)		Seating Chart and Flight Details
6	Scheduled Flight Date	Days Until Flight	Status	Cutoff Date	Days Remaining Until Cutoff Date	Arrival Date	Days Remaining Until Flight Arrival	Project	Project Status	# Of Seats Assigned (8 samples/seat)	Passengers Due In Terminal	Est'd Passenger Delivery (Data Release)
7	Tuesday, 03-01-22	Will depart in -125 days.	Arrived	Tuesday, 02-15-22	Cutoff date is past. This Charter-Seq flight is closed.	Friday, 04-01-22	Flight arrives in -94 days.	O'Connor_011	Departed	2
8								Aliota_003	Departed	5
9								Scott_004	Departed	6
10								Lou2_039	Departed	2
11								Leung2_012	Departed	3
12								Urriola_004	Departed	4
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19	Friday, 04-01-22	Will depart in -94 days.	Arrived	Friday, 03-18-22	Cutoff date is past. This Charter-Seq flight is closed.	Monday, 05-02-22	Flight arrives in -63 days.	VanBerlo_015	Departed	2
20								Li_005	Departed	2
21								Cvetanovic_016	Departed	2
22								Ingolfsland_005	Departed	5
23								Farrar_051	Departed	2
24								Prins_005	Departed	3
25								Kamdar_001	Departed	3
26								Saydam_001	Departed	2
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30	Monday, 05-02-22	Will depart in -63 days.	Arrived	Monday, 04-18-22	Cutoff date is past. This Charter-Seq flight is closed.	Thursday, 06-02-22	Flight arrives in -32 days.	Venteicher_028 (TB)	Departed	2
31								Marchant_029	Departed	6
32								French_001	Departed	3
33								Robbins2_035	Departed	3
34								Nelson3_011	Departed	3
35								Subramanian_051	Departed	4
36								Robertson3_025	Departed	2
37								Busta2_004	Departed	2
38								Dehm_082	Departed	2
39								Staley_084	Departed	3
40								Dougherty_004 (TB)	Departed	3
41								Thielen_007 (TB)	Departed	2
42								Stromnes_016	Departed	4
43								Stanton_003	Departed	2
44	Wednesday, 06-01-22	Will depart in -33 days.	In Flight	Friday, 05-13-22	Cutoff date is past. This Charter-Seq flight is closed.	Friday, 07-01-22	Flight arrives in -3 days.	Miller_044	Departed	5
45								Kassie_013	Departed	3
46								Malvick_002	Departed	2
47								Zordoky_064	Departed	2
48								O'Connor_012	Departed	4
49								Hackel_063	Departed	2
50								Ruan_028	Departed	3
51								Liu9_001	Departed	2
52								Sachs2_028	Departed	4
53								Farrar_053	Departed	2
54	Friday, 07-01-22	Will depart in -3 days.	Taking Reservations	Friday, 06-17-22	Cutoff date is past. This Charter-Seq flight is closed.	Monday, 08-01-22	Flight arrives in 28 days.	Klein_003	Pre-Flight Checks	3
55								Lange_034	Departed	5
56								Slattery_022	Pre-Flight Checks	4
57								Masopust_052	Departed	2
58								Huang9_002	Pre-Flight Checks	6
59								Nakato_004	Departed	2
60								Robertson3_026	Pre-Flight Checks	5
61								Slattery_023	Pre-Flight Checks	2
62								O'Connor_013	Departed	2
63								Moriarity_051	Pre-Flight Checks	6
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66	Monday, 08-01-22	Will depart in 28 days.		Monday, 07-18-22	You have 14 days remaining to submit samples for flight.	Thursday, 09-01-22	Flight arrives in 59 days.
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77
78	FAQ:
79	1) How do I reserve my seats and how many seats can I request?
80	Reserve your seats by emailing your request to next-gen@umn.edu. You can request as many seats as you need as long as you are purchase a minimum of 2 seats (16 samples) and groups of 8 samples thereafter. The flight has a maximum of 48 seats (384 samples).
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82	2) What are the charges?
83	Library preparation and sequencing @ 20M read depth is $179 for UofMN researchers. External users can board the flight for $205. Extra baggage (additional 20M reads) costs $100.
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85	3) What samples are acceptable?
86	We accept eukaryotic total RNA samples only for this service. No bacterial or Ribozero service is currently being offered.
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88	4) Do you perform QC on samples?
89	Yes, the UMGC provides QC services for all RNA-seq samples. Samples MUST be pre-validated before they are accepted for the Charter Service.
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91	5) What is the deadline for submission?
92	2 weeks (10 working days) prior to the flight date. This allows UMGC the opportunity to perform sample QC and obtain replacement samples, if necessary.
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94	6) Can I request Express Service for Charter-Seq?
95	There is no Express service. The dates are set.
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97	7) How do I request this service?
98	Please refer to this Google Doc to see the availibility of the next run.
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100	8) What if I miss the deadline for submission?
101	You will have the option of having your samples processed using our standard RNA-seq service and purchasing a separate lane of sequencing. Or, you can wait until the next Charter Service flight.
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103	9) This is great, but I can't seem to get on a Charter-Seq run because they fill up too fast.
104	We have the ability to increase the frequency of Charter Service runs, and will do so if we find that demand justifies it.
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106	10) I still have questions. Who can I contact?
107	Please contact Elyse Froehling or Aaron Becker at next-gen@umn.edu for questions regarding this service.