UMGC Sequencer Home


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2	HiSeq 2500 High-output Sample Queue (Samples being readied for sequencing on "Franklin").														This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
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4	1x50 Single Read Libraries							2x50 Paired-end Libraries
5	Project	# of Lanes	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB	Project	# of Lanes	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB	Explainer:
6	Langlois_009	1	7/2/2018	6/29/2018	TBD		YES	Dudley_001	1	Done	6/25/2018	Analysis		YES	The tables on the left report currently sequencing -- and future -- high-output sequencing libraries. The availability of libraries determines the timing of queued HiSeq 2500 runs, the planning of which is shown. The exact make-up of queued runs is liable to change depending on the timing of completion of libraries. INVESTIGATOR: the UMGC unique project ID, in format Investigator_### # OF LANES: the number of high-output lanes requested LIBRARY STATUS (EST’D DATE): the date when libraries are expected in the sequencing suite. These include both UMGC-created and client-created libraries, and the estimated date may change due to a number of factors. If the library is QC'd and ready to load, this cell will be marked 'Done'. QUEUED RUN NAME: the name of the run in which the libraries are or will be sequenced -- these names refer to current or queued runs shown on the 2500 High-output Runs page # LANES FILLED: the number of lanes (out of 8) that have been filled for queued runs. Green shading indicated a filled flow cell, whereas red shading indicated that there are too few libraries accumulated to permit the run to proceed. Yellow shading indicates a flow cell that is 6/8 or 7/8 full, and that we may decide to sequence if the libraries have been waiting a long time in queue. ESTIMATED RUN DATE: the date at which queued runs are estimated to be launched.
7	Vidovic_003	0.5	8/1/2018	8/8/2018	TBD		YES	Bemis_002	0.5	7/19/2018	8/3/2018	Analysis		YES
8	Finno2_015	2.5	8/24/2018		TBD		NO	Holding_010	1	8/16/2018		TBD		NO
9								Duan_007	2.5	8/17/2018		TBD		NO
10								Igarashi_010	1	8/20/2018		TBD		NO
11								Tran_002	1.5	8/20/2018		TBD		NO
12								Gordon_011	1	8/20/2018		TBD		NO
13								Jenkins_013	0.5	8/21/2018		TBD		NO
14								Georgieff_004	1	8/22/2018		TBD		NO
15								Schwertfeger_016	1	8/23/2018		TBD		NO
16								Jenkins_014	1	8/30/2018		TBD		NO
17								Georgieff_005	2	9/6/2018		TBD		NO
18								Bernlohr_055	1.5	10/10/2018
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27	10x Single-cell Paired-end SI 27x99 Libraries							2x125 Paired-end Libraries
28	Project	# of Lanes	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB	Project	# of Lanes	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB
29	Starr_059_90	1	5/17/2018	5/17/2018	Analysis		YES	Igarashi_009	1	7/17/2018	7/19/2018	Current B	8/8	YES
30	Starr_063_96	1	7/9/2018	7/9/2018	Analysis		YES	Dunny_044	0.5	7/31/2018	8/1/2018			YES
31	Starr_064_76	1	7/9/2018	7/9/2018	Analysis		YES	Phelps_022	1	7/18/2018	8/3/2018			YES
32	Starr_065_98	1	7/9/2018	7/9/2018	Analysis		YES	Behrens_004	2	8/3/2018	8/3/2018			YES
33	Starr_066_99	1	7/9/2018	7/9/2018	Analysis		YES	Knights_055 Pool 1	1	8/8/2018	8/8/2018			YES
34	Starr_067_100	1	7/9/2018	7/9/2018	Analysis		YES	Knights_055 Pool 2	1.5	8/8/2018	8/8/2018			YES
35	Starr_068_101	1	7/9/2018	7/9/2018	Analysis		YES	Hamilton2_011	1	8/9/2018	8/9/2018			YES
36	Starr_069_103	1	7/9/2018	7/9/2018	Analysis		YES	Finno2_014	3.5	8/13/2018	8/9/2018	TBD		YES
37	Starr_063_96_ReSeq	1.33	8/3/2018	8/3/2018	Analysis		YES	Tiffin_017/018	0.5	8/20/2018		TBD		NO
38	Starr_065_98_ReSeq	1.33	8/3/2018	8/3/2018	Analysis		YES	Blekhman_029	13	8/8/2018		TBD		NO
39	Starr_064_76_ReSeq	1.33	8/3/2018	8/3/2018	Analysis		YES	Saqui-Salces_002	2	8/20/2018		TBD		NO
40	Starr_070	1	8/20/2018		TBD		NO	Hozalski_006	1	8/15/2018		TBD		NO
41	Starr_073	1	8/20/2018		TBD		NO	Yang5_005	2	8/16/2018		TBD		NO
42	Starr_071	1	8/20/2018				NO	Azarin_001	1	8/17/2018		TBD		NO
43	Starr_074	1	8/20/2018				NO	Dehm_048	1	8/30/2018		TBD		NO
44	Perlingeiro_052_T1	0.3	8/20/2018					Koob_003	2	8/31/2019		TBD		NO
45	Perlingeiro_052_T2	0.3	8/20/2018					O'Connell_009	1	9/4/2018		TBD		NO
46	Perlingeiro_052_T3	0.3	8/20/2018					Banerjee2_008	1	8/15/2018		TBD		NO
47	Schwertfeger_017_Tumor	1	8/20/2018
48	Schwertfeger_017_Lung	1	8/20/2018
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3	Standard Libraries (First-come, First-served, MiSeqs #1-#2) Standard runs are ordinarily scheduled on MiSeq #1 or #2, but may be run on MiSeqs #3- #5 if those instruments are open.							Veterinary Diagnostic Lab (VDL) Libraries (MiSeq #3) VDL samples receive priority on MiSeq #3, which is owned by the VDL and operated by the UMGC. Doug Marthaler of the VDL (marth027@umn.edu) is the gatekeeper for all VDL libraries. MiSeq #3 is used for "Standard" runs only when the VDL does not have libraries ready for sequencing.								Expedited Libraries (MiSeq #4) For clients who need more rapid turnaround, MiSeq #4 -- un unsubsidized instrument -- is reserved for their use. Although runs on MiSeq #4 are about 50% more expensive than on MiSeq #1 or #2, there is typically no queue to get on the instrument. MiSeq #4 is used for "Standard" runs only when there are no expedited libraries ready for sequencing.							Harris Lab Libraries (MiSeq #5) Harris Lab samples receive priority on MiSeq #5, which is owned by Dr. Harris and operated by the UMGC. MiSeq #5 is used for "Standard" runs only when the Harris lab does not have libraries ready for sequencing.
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5	Project	Run Type	# of Lanes	Estimated Library Completion	Date Libraries received in CCRB	Run Status	MiSeq #	Project	Run Type	# of Lanes	Estimated Library Completion	Date Libraries received in Snyder	VDL Priority	Run Status	MiSeq #	Project	Run Type	# of Lanes	Estimated Library Completion	Date Libraries received in CCRB	Run Status	MiSeq #	Project	Run Type	# of Lanes	Estimated Library Completion	Date Libraries received in CCRB	Run Status	MiSeq #
6	PMS2_001_002	2x150 Nano Run (1M Reads)	1	8/17/2018	8/9/2018	1. Running	MiSeq #2	Mor_063/Mor_064/Mor_069 (3 samples)	2x300 Version 3 Chemistry (16-22M Reads)	1	8/1/2018		2	Data Analysis	MiSeq #3
7	MiSeq_Stowaway_051	2x300 Version 3 Chemistry (16-22M Reads)	1	8/17/2018		2. Queued Run #1	MiSeq #4	Mor_067/Mor_068	2x250 Version 2 Chemistry (8-12M Reads)	1			3	TBD	MiSeq #3
8	MiSeq_Stowaway_052	2x300 Version 3 Chemistry (16-22M Reads)	1	8/17/2018		2. Queued Run #1	MiSeq #5	Mor_070/Mor_071	2x250 Version 2 Chemistry (8-12M Reads)	1			4	TBD	MiSeq #3
9	LifeEGX_MnIP_004_Run_1	2x250 Nano Run (1M Reads)	1	7/25/2018	7/26/2018	Data Analysis		Mor_072/Mor_073	2x250 Version 2 Chemistry (8-12M Reads)	1	8/22/2018
10	LifeEGX_MnIP_004_Run_2	2x250 Nano Run (1M Reads)	1	7/25/2018	7/26/2018	Data Analysis		Mor_074/Mor_075	2x250 Version 2 Chemistry (8-12M Reads)	1	8/22/2018
11	Lin2_002	2x300 Version 3 Chemistry (16-22M Reads)	1	8/1/2018	8/1/2018	Data Analysis
12	Miller6_001_Steffenson_012_Nano_QC	2x300 Version 3 Chemistry (16-22M Reads)	1	8/7/2018	8/10/2018	Data Analysis
13	Perlingeiro_049/051+Perlingeiro_050_Nano_QC	2x150 Nano Run (1M Reads)	1	8/10/2018	8/10/2018	Data Analysis
14	Kashyap_012	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
15	LaPara_037_Run_1	2x250 Version 2 Chemistry (8-12M Reads)	1			TBD
16	LaPara_037_Run_2	2x250 Version 2 Chemistry (8-12M Reads)	1			TBD
17	LaPara_037_Run_3	2x250 Version 2 Chemistry (8-12M Reads)	1			TBD
18	LaPara_037_Run_4	2x250 Version 2 Chemistry (8-12M Reads)	1			TBD
19	Hieken_002	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
20	Chun_003_Run_1	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
21	Chun_003_Run_2	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
22	Chia_009	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
23	Walther-Antonio_002_Run_1	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
24	Walther-Antonio_002_Run_2	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
25	Tetel_001	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
26	Hamilton2_012	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
27	Walther-Antonio_003_Run_1	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
28	Walther-Antonio_003_Run_2	2x300 Version 3 Chemistry (16-22M Reads)	1			TBD
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47		# Future Lanes	23						# Future Lanes	5							# Future Lanes	0						# Future Lanes	0


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2	NovaSeq Run Calendar				Questions? E-mail: next-gen@umn.edu
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4	Flow cell position: A		Flow cell position: B		This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
5	Idle		Running
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7	Current Run		Current Run		Explainer: The tables on the left report on current and queued runs in the two flow cell positions (A and B) on the Illumina NovaSeq 6000 Sequencer. The sequencer can run two flow cells at a time. S1 and S2 flow cells have 2 lanes, while S4 flow cells have 4. One lane generates between 750M and 2250M reads, depending on which flow cell is used (S1, S2 or S4) resulting in the lowest-cost NGS data available. To view the queue of samples that are currently being prepared for sequencing on Nova, click on the NovaSeq Sample Queue tab above.
8	Start		Start	8/16/2018
9	End		End	8/18/2018
10	Elapsed		Elapsed	Has been running for 1 day.
11	Run Type		Run Type	S4 2x150-bp Paired-End DI
12	Run ID		Run ID	NovaSeq_Run_0030_08_16_18
13	Lane 1		Lane 1	Morrell_008
14	Lane 2		Lane 2	Morrell_008
15	Lane 3		Lane 3	Morrell_008
16	Lane 4		Lane 4	Morrell_008
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18	Queued Run A1		Queued Run B1		Queued Run Notes: Note 1: For queued runs, the Est'd Start date will be entered once all lanes are filled, or at such time that we decide to sequence the flow cell despite the fact that it is only partially filled. Note 2: Clients may be able to accelerate the running of a partially-filled flow cell by requesting an expansion of their sequencing to fill the empty lanes. To discuss adding additional lanes to a queued flow cell, E-mail: next-gen@umn.edu. Note 3: Libraries shown in yellow are not yet on hand in the sequencer suite, but are expected imminently, and have therefore been mapped to a run. Note 4: Libraries shown in purple are CLIA libraries
19	Est'd Start	8/17/2018	Est'd Start	8/20/2018
20	Est'd End	8/20/2018	Est'd End	8/22/2018
21	Run Type	S4 2x150-bp Paired-End DI	Run Type	S1 2x150-bp Paired-End DI
22	Lane 1	Morrell_009	Lane 1	Perlingeiro_049/050/051
23	Lane 2	Morrell_009	Lane 2	Magli_008
24	Lane 3	Morrell_009	Lane 3	NA
25	Lane 4	Morrell_009	Lane 4	NA
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27	Queued Run A2		Queued Run B2
28	Est'd Start		Est'd Start
29	Est'd End		Est'd End
30	Run Type		Run Type
31	Lane 1		Lane 1
32	Lane 2		Lane 2
33	Lane 3		Lane 3
34	Lane 4		Lane 4
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36	Queued Run A3		Queued Run B3
37	Est'd Start		Est'd Start
38	Est'd End		Est'd End
39	Run Type		Run Type
40	Lane 1		Lane 1
41	Lane 2		Lane 2
42	Lane 3		Lane 3
43	Lane 4		Lane 4


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2	NovaSeq Sample Queue (Projects sequencing, or in queue for sequencing).																		This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
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4	1x100 Single Read Libraries									2x50 Paired-end Libraries
5	Project	# of Lanes	Flow Cell Type	Loading Protocol	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB	Project	# of Lanes	Flow Cell Type	Loading Protocol	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB	Explainer
6	Pepsico_203	2	S2	Standard	8/7/2018		TBD	2/2	NO										The tables on the left report currently sequencing -- and future -- NovaSeq sequencing libraries. We are currently only selling NovaSeq runs as single lanes or full flow cells. Fractional lanes are not allowed except for special UMGC internal service runs The availability of libraries determines the timing of queued NovaSeq runs, the planning of which is shown. The exact make-up of queued runs is liable to change depending on the timing of completion of libraries. PROJECT: the UMGC unique project ID, in format Investigator_### # OF LANES: the number of lanes requested LIBRARY STATUS (EST’D DATE): the date when libraries are expected in the sequencing lab. These include both UMGC-created and client-created libraries, and the estimated date may change due to a number of factors. If the library is QC'd and ready to load, the date received will be marked int the column immediately to the right. QUEUED RUN NAME: the name of the run in which the libraries are or will be sequenced -- these names refer to current or queued runs shown on the NovaSeq Runs page # LANES FILLED: the number of lanes (of 2 or 4, depending on flow cell type) that have been filled for queued runs. Green shading indicated a filled flow cell, whereas red shading indicates that there are too few libraries accumulated to permit the run to proceed. ESTIMATED RUN DATE: the date at which queued runs are estimated to be launched.
7	GBS_Group_G	2	S1	Standard	8/15/2018		TBD	2/2	NO
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28	2x100 Paired-end Libraries									2x150 Paired-end ibraries
29	Project	# of Lanes	Flow Cell Type	Loading Protocol	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB	Project	# of Lanes	Flow Cell Type	Loading Protocol	Library Status (Est'd Date)	Date Libraries received in CCRB	Status	# Lanes Filled	Libraries Received in CCRB
30										UMGC_Project_532_Set_2	1	S2	XP	8/3/2018	8/3/2018	Analysis	2/2	YES
31										TSOE_048_049	1	S2	XP	8/10/2018	8/9/2018	Analysis	2/2	YES
32										Morrell_008	4	S4	Standard	8/7/2018	8/9/2018	Current B	4/4	YES
33										Morrell_009	4	S4	Standard	8/16/2018		Queued A1	4/4	NO
34										Perlingeiro_049/050/051	1	S1	XP	8/10/2018		Queued B2	2/2	NO
35										Magli_008	1	S1	XP	8/10/2018	8/13/2018	Queued B2	2/2	YES
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2	Stowaway-Seq Run Calendar.
3	Stowaway-Seq (2x300 PE MiSeq Runs) Stowaway-Seq is a service designed to provide shallow (≤ 2M reads), low-cost ($299.09) sequencing for small-scale pilot experiments. We achieve this by a) pooling projects from many different users, b) managing the addition of indexing barcodes to ensure that pooled libraries are compatible, and c) running all projects using a read length (2x300-bp PE) that encompasses any desired sequencing flavor. Please read FAQs below for more information, contact Corbin Dirkx (micbiom@umn.edu, 16S samples or amplicons) or Aaron Becker (becke044@umn.edu, other library types).
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5	Run Date and Status			Submission Cutoff Dates For Client-made Libraries		Submission Cutoff Dates For Amplicons or 16S Samples (Important: See FAQ #1!)		Run Details
6	Scheduled Run Date	Days Until Run	Status	Cutoff Date	Days Remaining Until Cutoff Date	Cutoff Date	Days Remaining Until Cutoff Date	Project	# of 1/8th Lanes Requested	# of 1/8th Lanes Granted	Date Libraries received in CCRB	MiSeq #
7	Saturday, 07-28-18	Will launch in -20 days.	Completed	Saturday, 07-14-18	Cutoff date is past. This Stowaway-Seq run is closed.	Saturday, 06-30-18	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Gale_015	1	1	7/27/2018	MiSeq #4
8								Hunter_018	1	2	7/24/2018
9								Kalan_001	1	1	7/24/2018
10								Clandinin_006	1	2	7/26/2018
11								Chen13_005	1	1	7/24/2018
12								Olson3_001	1	1	7/24/2018
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15	Friday, 08-17-18	Will launch in 0 days.	Next Up	Friday, 08-03-18	Cutoff date is past. This Stowaway-Seq run is closed.	Friday, 07-20-18	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Grywacz_001	1	1	8/3/2018	MiSeq #4
16								Hackel_019	1	1	8/16/2018
17								Hackel_020	1	1	8/16/2018
18								UMGC_IL_007	1	1
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23	Friday, 08-17-18	Will launch in 0 days.	Next Up	Friday, 08-03-18	Cutoff date is past. This Stowaway-Seq run is closed.	Friday, 07-20-18	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Staley_008	1	1		MiSeq #5
24								Baughn_019	1	1
25								Ruan2_002	1	1
26								Mansky_033	1	1
27								Herzberg_009	4	4
28								-	-	-
29								-	-	-
30								-	-	-
31	Friday, 09-07-18	Will launch in 21 days.		Friday, 08-24-18	You have 7 days remaining to submit client-made libraries.	Friday, 08-10-18	Cutoff date is past. This Stowaway-Seq run is closed for amplicon/16S sample submission.	Gorr_026	1	1
32								Pepsico_207	1	1
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39	Friday, 09-28-18	Will launch in 42 days.		Friday, 09-14-18	You have 28 days remaining to submit client-made libraries.	Friday, 08-31-18	You have 14 days remaining to submit amplicons or 16S samples.
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48	FAQ:
49	1) How much space can I request?
50	You may request increments of 1/8th of a MiSeq Lane to a maximum of 4 "lanes", if space allows. UMGC staff reserves the right to limit lane requests to a single "lane" per run, per user when high request volumes occur. If a large number of submissions are received, we will create multiple stowaway runs for a given submission period.
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52	2) What are the charges?
53	UMGC will charge users in increments of 1/8th of a lane ($299.09 per 1/8 th of a lane) requested.
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55	3) What samples are acceptable?
56	Libraries that have been indexed and pooled will be accepted two weeks before the Stowaway-Seq run date. We may accept samples for library creation for Stowaway service (e.g., amplicons or 16S samples), but ONLY when prior approval has been obtained, with enough time for the library creation to occur.
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58	4) Do you perform QC on libraries?
59	Yes. We perform Kapa QC on samples to ensure functional library.
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61	5) What is the deadline for submission?
62	For client-made libraries, the cut-off for submission is at least 14 days prior to the scheduled run. For amplicons and 16S samples, the cut-off for submission is at least 28 days prior to the scheduled run.
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64	6) Can I request Expedited Service for a Stowaway run?
65	No. The purpose of Stowaway-Seq is not speed, but low cost. The runs are scheduled in advance and guaranteed to run on the scheduled date, in order to provide a reliable, low-cost option for all.
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67	7) How do I request this service?
68	Please refer to this Google Doc to see the availibility of the next run.
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70	8) How can I know what barcodes to use for my samples – so that they do not overlap with another researcher’s barcodes?
71	Please email next-gen@umn.edu with your intended barcodes. We will check it against others currently in the run and notify you.
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73	9) What if I miss the deadline for submission?
74	UMGC will try to accommodate your request on the next scheduled run provided there is no barcode conflict.
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76	10) This is great, but I can't seem to get on a Stowaway-Seq run because they fill up too fast, and my submission type is of low priority according to your policies!!!
77	We have the ability to increase the frequency of Stowaway-Seq runs, and will do so if we find that demand justifies it.
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79	11) I still have questions. Who can I contact?
80	Please contact the Aaron Becker (Illumina Sequencing Services Manager) at becke044@umn.edu for questions regarding client-made library submission, or Corbin Dirkx (High-throughput Sequencing Services Manager) at micbiom@umn.edu for questions regarding amplicon or 16S sample submission.


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2	High-output HiSeq 2500 ("Franklin") Run Calendar			Questions? E-mail: next-gen@umn.edu
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4	Flow cell position: A	Flow cell position: B		This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
5	Under Repair	Running
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7	Current Run	Current Run
8	Start	Start	8/15/2018
9	End	End	8/21/2018
10	Elapsed	Elapsed	Has been running for 2 days.
11	Run Type	Run Type	2x125-bp Paired-End DI
12	Run ID	Run ID	HS4_FC_B_08_14_18
13	Lane 1	Lane 1	Igarashi_009
14	Lane 2	Lane 2	Phelps_022
15	Lane 3	Lane 3	Behrens_004 (0.66)/Hamilton2_011 (0.33)
16	Lane 4	Lane 4	Behrens_004 (0.66)/Hamilton2_011 (0.33)
17	Lane 5	Lane 5	Behrens_004 (0.66)/Hamilton2_011 (0.33)
18	Lane 6	Lane 6	Knights_055 Pool 1
19	Lane 7	Lane 7	Knights_055 Pool 2
20	Lane 8	Lane 8	Knights_055 Pool 2/Dunny_044*
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22	Queued Run A1	Queued Run B1
23	Est'd Start	Est'd Start
24	Est'd End	Est'd End
25	Run Type	Run Type
26	Lane 1	Lane 1
27	Lane 2	Lane 2
28	Lane 3	Lane 3
29	Lane 4	Lane 4
30	Lane 5	Lane 5
31	Lane 6	Lane 6
32	Lane 7	Lane 7
33	Lane 8	Lane 8
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35	Queued Run A2	Queued Run B2
36	Est'd Start	Est'd Start
37	Est'd End	Est'd End
38	Run Type	Run Type
39	Lane 1	Lane 1
40	Lane 2	Lane 2
41	Lane 3	Lane 3
42	Lane 4	Lane 4
43	Lane 5	Lane 5
44	Lane 6	Lane 6
45	Lane 7	Lane 7
46	Lane 8	Lane 8
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48	Queued Run A3	Queued Run B3
49	Est'd Start	Est'd Start
50	Est'd End	Est'd End
51	Run Type	Run Type
52	Lane 1	Lane 1
53	Lane 2	Lane 2
54	Lane 3	Lane 3
55	Lane 4	Lane 4
56	Lane 5	Lane 5
57	Lane 6	Lane 6
58	Lane 7	Lane 7
59	Lane 8	Lane 8
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61	Queued Run A4	Queued Run B4
62	Est'd Start	Est'd Start
63	Est'd End	Est'd End
64	Run Type	Run Type
65	Lane 1	Lane 1
66	Lane 2	Lane 2
67	Lane 3	Lane 3
68	Lane 4	Lane 4
69	Lane 5	Lane 5
70	Lane 6	Lane 6
71	Lane 7	Lane 7
72	Lane 8	Lane 8


1
2		MiSeq #1 (Standard Sample Queue)	MiSeq #2 (Standard Sample Queue)		MiSeq #3 (VDL MiSeq) (Priority: VDL)		MiSeq #4 (Priority: Expedited Runs)		MiSeq #5 (Harris Lab MiSeq) (Priority: Harris Lab)
3		Idle	Running		Idle		Idle		Idle
4
5	Current Run	Start	Start	8/17/2018	Start		Start		Start
6		End	End	8/18/2018	End		End		End
7		Read Length	Read Length	2x250 Nano Run (1M Reads)	Read Length		Read Length		Read Length
8		Queue Type	Queue Type	Standard Sample Queue	Queue Type		Queue Type		Queue Type
9		Projects	Projects	PMS2_001/002	Projects		Projects		Projects
10
11	Queued Run #1	Start	Start		Start	8/16/2018	Start	8/17/2018	Start	8/17/2018
12		End	End		End	8/18/2018	End	8/20/2018	End	8/20/2018
13		Read Length	Read Length		Read Length	2x250 Version 2 Chemistry (8-12M Reads)	Read Length	2x300 Version 3 Chemistry (16-22M Reads)	Read Length	2x300 Version 3 Chemistry (16-22M Reads)
14		Queue Type	Queue Type		Queue Type	VDL Sample Queue	Queue Type	Standard Sample Queue	Queue Type	Standard Sample Queue
15		Projects	Projects		Projects	Mor_067/Mor_068	Projects	MiSeq_Stowaway_051	Projects	MiSeq_Stowaway_052
16
17	Queued Run #2	Start	Start		Start		Start		Start
18		End	End		End		End		End
19		Read Length	Read Length		Read Length		Read Length		Read Length
20		Queue Type	Queue Type		Queue Type		Queue Type		Queue Type
21		Projects	Projects		Projects		Projects		Projects
22
23	Queued Run #3	Start	Start		Start		Start		Start
24		End	End		End		End		End
25		Read Length	Read Length		Read Length		Read Length		Read Length
26		Queue Type	Queue Type		Queue Type		Queue Type		Queue Type
27		Projects	Projects		Projects		Projects		Projects
28


1
2		NextSeq #1		Questions? E-mail: next-gen@umn.edu
3		Running
4				This is a web-published Google Sheet best viewed using Google Chrome or Apple Safari browsers. Firefox is not recommended.
5	Current Run	Start	8/16/2018
6		End	8/17/2018
7		Read Length	2x150 Mid (130M Reads)	Explainer: The table on the left reports current and queued runs on the NextSeq. The NextSeq can run one flow cell at a time in various flexible length configurations and outputs (High-output, Mid-output, paired-end and single read). Each flow cell will be assigned a single client project except in the case of GBS pilot runs. These runs are assigned as shared runs by UMGC staff. NextSeq runs have a duration of 1 to 2 days and are ideal for small to mid-output NGS projects. Expedited runs are available on the platform for an expedited fee. To view the queue of samples that are currently being prepared for sequencing, click on the NextSeq Sample Queue tab above.
8		Queue Type	Standard Sample Queue
9		Projects	Barker2_001
10
11	Queued Run #1	Start	8/17/2018
12		End	8/18/2018
13		Read Length	2x150 Mid (130M Reads)
14		Queue Type	Standard Sample Queue
15		Projects	LifeEGX_MnIP
16
17	Queued Run #2	Start
18		End
19		Read Length
20		Queue Type
21		Projects
22
23	Queued Run #3	Start
24		End
25		Read Length
26		Queue Type
27		Projects


1
2	NextSeq Standard Sample Queue							NextSeq Expedited Sample Queue
3
4	Project	Run Type	# of Lanes	Millions of Reads	Estimated Library Completion	Date Libraries received in CCRB	Run Status	Project	Run Type	# of Lanes	Millions of Reads	Estimated Library Completion	Date Libraries received in CCRB	Run Status
5	Barker2_001	2x150 Mid (130M Reads)	1	130	8/16/2018	8/15/2018	1. Running
6	LifeEGX_MnIP	2x150 Mid (130M Reads)	1	130	8/17/2018		2. Queued Run #1
7	Pankow_004_2	2x150 High (400M Reads)	1	400	7/4/2018	7/13/2018	Analysis
8	Pankow_004_2_Re_Seq	2x150 High (400M Reads)	1	400	7/18/2018	7/18/2018	Analysis
9	Pankow_004_3	2x150 Mid (130M Reads)	1	130	7/11/2018		TBD
10	UMGC_IL_Project_005	2x150 High (400M Reads)	1	400	8/17/2018		TBD
11	Thompson3_Project_002	1x150 High (400M Reads)	1	400	8/21/2018		TBD
12	Moeller_Project_005	1x150 High (400M Reads)	1	400	8/22/2018		TBD
13	Bielinsky_Project_008	2x75 Mid (130M Reads)	1	130	8/31/2018		TBD
14
15
16
17
18
19
20
21
22
23
24
25		# Future Lanes	9						# Future Lanes	0