20151109 - RLOv ISH Probe PCRs
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pCR2.1/RLOv_membrane_gene_1DIG Probe
Unlabeled Control
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CiCfVol. (uL)Vol. (uL)
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H2O10.648.14
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PCR Buffer (vial 3)1012.502.50
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DIG Probe Synthesis Mix (vial 2)1012.50
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dNTP Stock Solution (vial 4)1012.50
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Pf (RLOv_membrane_1_F)1012.502.50
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Pr (RLOv_membrane_1_R)1012.502.50
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Enzyme Mix (vial 1)66.610.380.38
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pCR2.1/RLOv_membrane_gene_1 (pg/uL)
2.5110.43.983.98
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Control Template (vial 5)1012.50
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Total2525
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pCR2.1RLOv_membrane_gene_2
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DIG Probe
Unlabeled Control
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CiCfVol. (uL)Vol. (uL)
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H2O11.098.59
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PCR Buffer (vial 3)1012.502.50
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DIG Probe Synthesis Mix (vial 2)1012.50
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dNTP Stock Solution (vial 4)1012.50
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Pf (RLOv_membrane_2_F)1012.502.50
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Pr (RLOv_membrane_2_R)1012.502.50
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Enzyme Mix (vial 1)66.610.380.38
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pCR2.1/RLOv_membrane_gene_2 (pg/uL)
2.8260.43.543.54
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Control Template (vial 5)1012.50
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Total2525
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pCR2.1RLOv_tail_fiber
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DIG Probe
Unlabeled Control
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CiCfVol. (uL)Vol. (uL)
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H2O10.367.86
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PCR Buffer (vial 3)1012.502.50
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DIG Probe Synthesis Mix (vial 2)1012.50
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dNTP Stock Solution (vial 4)1012.50
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Pf (RLOv_tail_fiber_F)1012.502.50
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Pr (RLOv_tail_fiber_R)1012.502.50
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Enzyme Mix (vial 1)66.610.380.38
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pCR2.1/RLOv_tail_fiber (pg/uL)2.3440.44.274.27
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Control Template (vial 5)1012.50
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Total2525
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