recombinant data 2017
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ABCDEFGHIJKLMNOPQRSTU
1
band sizes (kb)
2
gelGlaneplasmid #T or SSD or DDband 1band 2other bandsinterpreted recombinantComments (ud = undigested, id = incomplete digest)
3
111ld
4
1121SSD6.6pUC18 (L) + pKC7 (L)
5
1131SDD42.6
6
1142SSD4.5pUC18 (L) + GeneA
7
1152SDD2.61.8
8
1163SSD4pKC7 (L) + 30bp pUC18
9
1173SDD4
10
1184SSD6.642.6other (e.g. 4 fragments)ID
11
1194SDD2.64
12
11105SSD4.5pUC18 (L) + GeneA
13
11115SDD1.82.6
14
11126SSD6.642.6other (e.g. 4 fragments)
15
11136SDD42.6
16
11147SSD4pKC7 (L) + 30bp pUC18
17
11157SDD4
18
211ld
19
2128SSD4.5pUC18 (L) + GeneA
20
2138SDD1.82.6
21
2149SSD6pKC7 (R)
22
2159SDD42
23
21610SSD6pKC7 (R)
24
21710SDD42
25
2181TSD4.5pUC18 (L) + GeneA
26
2191TDD1.82.6
27
21102TSD4.5pUC18 (L) + GeneA
28
21112TDD1.82.6UD
29
321ld
30
3221SSD5.8pKC7 (R)
31
3231SDD41.8
32
3242SSD5.8pKC7 (R)
33
3252SDD41.8
34
3263SSD5.8pKC7 (R)
35
3273SDD41.8
36
3284SSD5.8pKC7 (R)
37
3204SDD41.8
38
32105SSD5.8pKC7 (R)
39
32115SDD41.8
40
32126SSD5.8pKC7 (R)
41
32136SDD41.8
42
32147SSD2.7pUC18 (R)This should have been a blue colony
43
32157SDD2.7
44
421ld
45
4228SSD6.5pUC18 (L) + pKC7 (L)
46
4238SDD42.7
47
4249SSDcan't tell
48
4259SDD
49
42610SDDcan't tell
50
42710SSD
51
4281TSD4.5pUC18 (L) + GeneA
52
4201TDD2.51.8
53
42102TSD4.5pUC18 (L) + GeneA
54
42112TDD2.51.8
55
51ld
56
5321SSD6pKC7 (R)
57
5331SDD41.8
58
5342SSD6.6pUC18 (L) + pKC7 (L)
59
5352SDD42.6
60
5363SSD4pKC7 (L) + 30bp pUC18
61
5373SDD4
62
5384SSD6.6pUC18 (L) + pKC7 (L)
63
5304SDD42.6
64
53105SSD6.74other (e.g. 4 fragments)2.7 + 4 + 4 + 30bp
65
53115SDD42.6
66
53126SSD4pKC7 (L) + 30bp pUC18
67
53136SDD4
68
53147SSD4.51.8other (e.g. 4 fragments)1.8 + 1.8 + 2.7 + 30 BP
69
53157SDD2.71.8
70
631ld
71
6328SSD6pKC7 (R)
72
6338SDD41.8
73
6349SSD4pKC7 (L) + 30bp pUC18
74
6359SDD4
75
63610SSD6.5pUC18 (L) + pKC7 (L)
76
63710SDD42.5
77
6381TSDcan't tellincomplete digest
78
6301TDDid
79
63102TSD4.5pUC18 (L) + GeneAincomlete digest but can tell what it is
80
63112TDD3.72.51.8
81
741ld
82
7421SSD6.54.4pKC7 (R)id, partial
83
7431SDD1.84.4id
84
7442SSD4pKC7 (L) + 30bp pUC18
85
7452SDD4
86
7463SSD4pKC7 (L) + 30bp pUC18
87
7473SDD4
88
7484SSD7.2pUC18 (L) + pKC7 (L)
89
7494SDD42.8
90
74105SSD4pKC7 (L) + 30bp pUC18
91
74115SDD4
92
74126SSD4.51.8other (e.g. 4 fragments)pUC18(L) + Gene A + Gene A + 30 bp pUC18
93
74136SDD2.71.8
94
74147SSD4.6pUC18 (L) + GeneA
95
74157SDD2.71.8
96
841ld
97
8428SSD4.4pUC18 (L) + GeneA
98
8438SDD1.82.74id
99
8449SSD4pKC7 (L) + 30bp pUC18
100
8459SDD4
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