2015_03_31 Probe autoinduction conditions for ACS purification - vary time at RT, percent glycerol

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1	2015_03_31 Probe autoinduction conditions for ACS purification - vary time at RT, percent glycerol
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3	tube	tube mL	mL medium	mL glycerol	strain	uL glycerol	mL air	percent glycerol added	percent glycerol	inoculation time
4	1	50	2		BL21(DE3) pET ACS L641P		48		0.58	3/31/2015 15:25:00
5	2	50	10		BL21(DE3) pET ACS L641P		40		0.58	3/31/2015 15:25:00
6	3	50	25		BL21(DE3) pET ACS L641P		25		0.58	3/31/2015 15:25:00
7	4	50	40		BL21(DE3) pET ACS L641P		10		0.58	3/31/2015 15:25:00	60	percent glycerol stock
8	5	50	10	0	BL21(DE3) pET ACS L641P	0	40	0	0.58	3/31/2015 15:25:00	11/14? pg 534	glycerol stock prep date
9	6	50	10	0.05	BL21(DE3) pET ACS L641P	50	40	0.30	0.88	3/31/2015 15:25:00	autoclave	prep method
10	7	50	10	0.1	BL21(DE3) pET ACS L641P	100	40	0.59	1.17	3/31/2015 15:25:00
11	8	50	10	0.2	BL21(DE3) pET ACS L641P	200	40	1.18	1.75	3/31/2015 15:25:00
12	9	50	10	0.5	BL21(DE3) pET ACS L641P	500	40	2.86	3.43	3/31/2015 15:25:00
13	10	50	10	1	BL21(DE3) pET ACS L641P	1000	40	5.45	6.03	3/31/2015 15:25:00
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17	Note: The recipe I have been using has no magnesium. Helen is going to make a stock then test whether adding it helps.
18	Make a master stock of inoculum.
19	137	mL required
20	1.15	excess factor			Inoculum = ACS L641P restreaked from -80oC on 2/23 (~5 weeks ago!) and grown overnight at RT in LB + 2uL/mL Km
21	158	mL to prepare
22	0.01	overnight ratio
23	1.58	mL overnight			NanoDrop OD at inoculation:
24	159	final mL
25	318	uL Km
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