CRISPR Reagents

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	Y	Z	AA	AB	AC	AD	AE	AF	AG
1	Name	Batch #	Library Type	Species	Target	Description	Description	Total genes	Total gRNAs	Backbone	Selection	Vector system	Source	Acquisition	Location	Maxiprep	Lenti/Titer (IU/ml)	Cell pool	Design year	Representation	Notes	Library	Date	Sample	QID	Type	Viral QID	Titer IU/mL	Viral QID	Titer IU/mL

2	Calabrese		Activation	Human	Genome-wide	Set A, Genome-wide CRISPR gRNA pooled library for activation of human genes	3-6	18,885	56,762	pXPR_502	Puro	2 vector	Broad GPP genome wide	Addgene 92379	GEiC	500 ng/ul			2018			MSPH.1	20210223		337	Plasmid	214	2.30E+08
3	Calabrese		Activation	Human	Genome-wide	Set B, Genome-wide CRISPR gRNA pooled library for activation of human genes	3-6	18,843	56,476	pXPR_502	Puro	2 vector	Broad GPP genome wide	Addgene 92380	GEiC	500 ng/ul			2018			MSPH.2	20210713		217	Plasmid	238	8.21E+09
4	SAMv2		Activation	Human	Genome-wide	Genome-wide human CRISPR activation library	3	23,430	70,290	LentiSAMv2	Blast	2 vector	Feng Zhang	Addgene 1000000078	GEiC	500 ng/ul			2017			MSPH.3	20220830		215	Plasmid	240	3.00E+08	414	1.30E+07
5	M302.3		CRISPRa	Human	Metabolic S1	Reactome metab geness	4	23,430	93720													MSPH.4	20210810		218	Plasmid	242	1.95E+08	419	5.80E+08
6	M302.6		CRISPRa	Human	Metabolic S2	Metabolic atlas genes	4	23,430	93720													MSPH.5	20210810		219	Plasmid	327	5.73E+09
7	M302.9		CRISPRa	Human	Metabolic S3	Pagliarini Ext	4	765	3060													MSPH.6	20210810		220	Plasmid	244	3.00E+08
8	M302.12		CRISPRa	Human	Metabolic S4	Sabatini MSeqDR	4	765	3060													MSPH.7	20210720		338	Plasmid	216	5.90E+08	341	5.28E+07
9	M302.14		CRISPRa	Human	Mito S1	MPSH genes 1	4	765	3060													MSPH.1-4	20220929		446	Plasmid
10	M302.16		CRISPRa	Human	Mito S2	MPSH genes 2	4	765	3060													MSPH.1-7	20220929		447	Plasmid
11	M302.2		CRISPRi	Human	Metabolic S1	Reactome metab geness	4	765	3060													M302.25	20220913	41-A06	354	Plasmid	356	6.09E+05
12	M302.5		CRISPRi	Human	Metabolic S2	Metabolic atlas genes	4	765	3060													M302.26	20220913	41-A07	355	Plasmid	357	8.91E+07
13	M302.8		CRISPRi	Human	Metabolic S3	Pagliarini Ext	4	765	3060													M302.32	20220913	41-A08	445	Plasmid
14	M302.11		CRISPRi	Human	Metabolic S4	Sabatini MSeqDR	4	765	3060
15	M302.13		CRISPRi	Human	Mito S1	MPSH genes 1	4	350	1400
16	M302.15		CRISPRi	Human	Mito S2	MPSH genes 2	4	625	2500
17	Weissman Libraries		CRISPRi and CRISPRa - 2-3K genes/subpool, 7 subpools -> Kinases phosphotases and drug targets, cancer and apoptosis, stress and proteostasis, mitochondria trafficking motility, membrane proteins, gene expression, unassigned
18	Dolomiti		Inhibition	Mouse	Genome-wide	Set A, Genome-wide CRISPR gRNA pooled library for inhibition of mouse genes	3-6	22,311	67,366	pXPR_050	Puro	2 vector	Broad GPP genome wide	Addgene 104090	GEiC	500 ng/ul			2018
19	Dolomiti		Inhibition	Mouse	Genome-wide	Set B, Genome-wide CRISPR gRNA pooled library for inhibition of mouse genes	3-6	22,247	67,194	pXPR_050	Puro	2 vector	Broad GPP genome wide	Addgene 104091	GEiC	500 ng/ul			2018
20	Dolcetto		Inhibition	Human	Genome-wide	Set A, Genome-wide CRISPR gRNA pooled library for inhibition of human genes	3-6	18,901	57,050	pXPR_050	Puro	2 vector	Broad GPP genome wide	Addgene 92385	GEiC	500 ng/ul	Yes		2018
21	Dolcetto		Inhibition	Human	Genome-wide	Set B, Genome-wide CRISPR gRNA pooled library for inhibition of human genes	3-6	18,899	57,011	pXPR_050	Puro	2 vector	Broad GPP genome wide	Addgene 92386	GEiC	500 ng/ul	Yes		2018
22	Brunello		Knockout	Human	Genome-wide	Genome-wide CRISPR gRNA pooled library for knockout of human genes	4	19,114	76,441	lentiGuide-Puro	Puro	2 vector	Broad GPP genome wide	Addgene 73178	GEiC	500 ng/ul			2016
23	Brie		Knockout	Mouse	Genome-wide	Genome-wide CRISPR gRNA pooled library for knockout of mouse genes	4	19,674	78,637	lentiGuide-Puro	Puro	2 vector	Broad GPP genome wide	Addgene 73633	GEiC	500 ng/ul			2016
24	Gecko		Knockout	Human	Genome-wide	Genome-wide human CRISPR knockout pooled library (GeCKO v2)	6	19,050	123,411	lentiGuide-Puro	Puro	2 vector	Feng Zhang	Addgene 1000000049	GEiC	500 ng/ul			2014		First geneation library design, low performance
25	MSPH.14		Knockout	Human	Mitochondria	Mitochondria associated genes	4	12	50	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
26	MSPH.15		Knockout	Human	Mitochondria	Mitochondria CRISPR library	4	25	105	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
27	MSPH.16		Knockout	Human	Mitochondria	Mitochondria CRISPR library	4	51	210	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
28	MSPH.17		Knockout	Human	Mitochondria	Mitochondria CRISPR library	4	99	415	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
29	MSPH.18		Knockout	Human	Mitochondria	Mitochondria CRISPR library	4	39	827	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS, iCas9 iPSC	2019
30	MSPH.19		Knockout	Human	Mitochondria	Mitochondria CRISPR library	4	393	1650	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS, iCas9 iPSC	2019
31	MSPH.20		Knockout	Human	Mitochondria	Mitochondria CRISPR library	4	614	2578	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
32	MSPH.21		Knockout	Human	BRCA1	BRCA1 library targeting clinvar variant sites	13	1	13	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul			2019
33	M715.SP8		Knockout	Human	Human Mito Addn	Mitochondria CRISPR library	4	19	76	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
34	MSPM.2		Knockout	Mouse	Mitochondria	Mitochondria CRISPR library	4	38	152	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes		2019
35	MSPM.3		Knockout	Mouse	Mitochondria	Mitochondria CRISPR library	4	51	204	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes		2019
36	MSPM.4		Knockout	Mouse	Mitochondria	Mitochondria CRISPR library	4	100	400	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes		2019
37	MSPM.5		Knockout	Mouse	Mitochondria	Mitochondria CRISPR library	4	198	792	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes		2019
38	MSPM.6		Knockout	Mouse	Mitochondria	Mitochondria CRISPR library	4	389	1556	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes		2019
39	MSPM.7		Knockout	Mouse	Mitochondria	Mitochondria CRISPR library	4	706	2824	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes		2019
40	M302.1		KO	Human	Metabolic S1	Reactome metab geness	4	450	1800
41	M302.4		KO	Human	Metabolic S2	Metabolic atlas genes	4	1403	5612
42	M302.7		KO	Human	Metabolic S3	Pagliarini Ext	4	765	3060
43	M302.10		KO	Human	Metabolic S4	Sabatini MSeqDR	4	1855	7420
44	MSPH.33		Scanning	Human	Mitochondria	MFN2 library targeting clinvar variant sites	31	1	31	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
45	MSPH.34		Scanning	Human	Mitochondria	MFN2 library targeting clinvar variant sites	340	1	340	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
46	M1213.5		Scanning	Human	Mitochondria	Scanning (SLC25A19, ATAD3A)	112.5	2	225	lentiGuide-Puro	Puro	2 vector	Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	500 ng/ul	Yes	iCas9 U2OS	2019
47	MSPH.1	LSS11				Hs Mito Gene KO		12	50	https://wustl.box.com/s/uft4txddqgeml6qo49gxkke2u61d47m6	Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	6.7 X 10^8 (1-4 mix); 5.6 X 10^8 (1-7 mix)			https://wustl.box.com/s/autj94vl69dd1mdecewchxx1osva5w16	Josh langmade took 6 vials on 09/15/22
48	MSPH.2	LSS12				Hs Mito Gene KO		25	105	https://wustl.box.com/s/uft4txddqgeml6qo49gxkke2u61d47m6	Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	6.7 X 10^8 (1-4 mix); 5.6 X 10^8 (1-7 mix)			https://wustl.box.com/s/44wznr97jzpz52rd8zznqnetvxenh5ui	Josh langmade took 6 vials on 09/15/22
49	MSPH.3	LSS13				Hs Mito Gene KO		50	210	https://wustl.box.com/s/uft4txddqgeml6qo49gxkke2u61d47m6	Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	6.7 X 10^8 (1-4 mix); 5.6 X 10^8 (1-7 mix)			https://wustl.box.com/s/0os1gmn9igu60q3ff0c3nga5yt5w3x0u	Josh langmade took 6 vials on 09/15/22
50	MSPH.4	LSS14				Hs Mito Gene KO		100	415	https://wustl.box.com/s/uft4txddqgeml6qo49gxkke2u61d47m6	Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	6.7 X 10^8 (1-4 mix); 5.6 X 10^8 (1-7 mix)			https://wustl.box.com/s/7pudlptk9wx19px1fqaferlrzvqjqn8d	Josh langmade took 6 vials on 09/15/22
51	MSPH.5	LSS9				Hs Mito Gene KO		200	827	https://wustl.box.com/s/uft4txddqgeml6qo49gxkke2u61d47m6	Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	1.2 X 10^9 IU/ml			https://wustl.box.com/s/vexeqblxrd34dzuk2z7fin0nh5mspxmx	Josh langmade took 6 vials on 09/30/22
52	MSPH.6	LSS15				Hs Mito Gene KO		400	1650	https://wustl.box.com/s/uft4txddqgeml6qo49gxkke2u61d47m6	Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	1.2 X 10^9 IU/ml			https://wustl.box.com/s/1h1nukb9uro8zo8mjpu6cpt2agujfr7b	Josh langmade took 6 vials on 09/30/22
53	MSPH.7	LSS10				Hs Mito Gene KO		623	2578	https://wustl.box.com/s/uft4txddqgeml6qo49gxkke2u61d47m6	Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	1.4 X 10^9 IU/ml			https://wustl.box.com/s/vvzreht19xh2pxh7v3xn8n8qf7mv00au	Josh langmade took 6 vials on 09/30/22
54	M302.1	LSS4							1346 (Confirm with Willie)	https://wustl.box.com/s/x8m5otjz1kcpmxlxif92ctyjsdcaxhc7	Puro/BFP		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	1.1 X 10^9 IU/ml			https://wustl.box.com/s/ytlm4vs6hssro56lwe5gx2eezn7wjz8k
55	M302.28	LSS4							5871 (Confirm with Willie)	https://wustl.box.com/s/x8m5otjz1kcpmxlxif92ctyjsdcaxhc7	Puro/BFP		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul	sent to Mingjie on 10/20/22			https://wustl.box.com/s/91c3qb35co715k7pda2w78bzbyy5vkoc
56	M302.25	LSS4							2292 (Confirm with Willie)	https://wustl.box.com/s/x8m5otjz1kcpmxlxif92ctyjsdcaxhc7	Puro/BFP		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	837 ng/ul	sent to Mingjie on 10/20/22			https://wustl.box.com/s/7fny0axw2bt3cs27wx0sz418409yng1s
57	M302.12	LSS17							5354 (Confirm with Wille)	https://wustl.box.com/s/x8m5otjz1kcpmxlxif92ctyjsdcaxhc7	Puro/BFP		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul				https://wustl.box.com/s/oiwlx6pzqqabmqki6znwhu2zdzmm5gnj
58	M302.29	LSS18							2190 (Confirm with Willie)	https://wustl.box.com/s/x8m5otjz1kcpmxlxif92ctyjsdcaxhc7	Puro/BFP		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul				https://wustl.box.com/s/sw69hh5nak07m0r1nut1ak5mihzrsht1
59	M302.30	LSS19							4488 (Confirm with Willie)	https://wustl.box.com/s/x8m5otjz1kcpmxlxif92ctyjsdcaxhc7	Puro/BFP		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	1000 ng/ul				https://wustl.box.com/s/iitpxwkauqqz90ymozsmtw6gxn8yy4pl
60	M302.26	Rusiko									Puro		Buchser/GEiC	FIVE/GEIC	FIVE/GEIC	need amplification	sent to Mingjie on 10/20/22			Ask Willie
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62	Haney/Bassik knockout libraries (https://www.addgene.org/pooled-library/bassik-human-crispr-knockout/)
63	101927	6-22-23	knockout	Human	DTK	CRISPR Deletion Library - Drug targets, kinases, phosphatases†	Bassik 2_DTK	2333	24569	https://www.addgene.org/89359/	Puro/mCherry		2				8.6 X 10^8				Josh Langmade has all frozen aliquots
64	101926	7-24-23	knockout	Human	ACOC	CRISPR Deletion Library - Apoptosis and cancer†	Bassik 1_ACOC	3015	31324	https://www.addgene.org/89359/	Puro/mCherry		1				1.8 X 10^9				Josh Langmade has all frozen aliquots
65	101928	7-24-23	knockout	Human	GEEX	CRISPR Deletion Library - Gene expression†	Bassik 3_GEEX	2345	24449	https://www.addgene.org/89359/	Puro/mCherry		3				1.2 X 10^9				Josh Langmade has all frozen aliquots
66	101931	7-24-23	knockout	Human	TMMO	CRISPR Deletion Library - Trafficking, mitochondrial, motility†	Bassik 6_TMMO	2252	23615	https://www.addgene.org/89359/	Puro/mCherry		6				2 X 10^8				Josh Langmade has all frozen aliquots
67	101929	7-27-23	knockout	Human	MEPR	CRISPR Deletion Library - Membrane proteins†	Bassik 4_MEPR	1098	12334	https://www.addgene.org/89359/	Puro/mCherry		4				8.8 X 10^8				Josh Langmade has all frozen aliquots
68	101930	8-10-23	knockout	Human	PROT	CRISPR Deletion Library - Proteostasis†	Bassik 5_PROT	2927	30423	https://www.addgene.org/89359/	Puro/mCherry		5				1 X 10^9				Josh Langmade has all frozen aliquots
69	101932	8-10-23	knockout	Human	UNA1	CRISPR Deletion Library - Unassigned 1†	Bassik 7_UNA1	1954	20482	https://www.addgene.org/89359/	Puro/mCherry		7				1.2 X 10^9				Josh Langmade has all frozen aliquots
70	101933	8-17-23	knockout	Human	UNA2	CRISPR Deletion Library - Unassigned 2†	Bassik 8_UNA2	2771	28187	https://www.addgene.org/89359/	Puro/mCherry		8				1 X 10^9				Josh Langmade has all frozen aliquots
71	101934	8-17-23	knockout	Human	UNA3	CRISPR Deletion Library - Unassigned 3†	Bassik 9_UNA3	1854	17438	https://www.addgene.org/89359/	Puro/mCherry		9				1.2 X 10^9				Josh Langmade has all frozen aliquots
72	NEW PREPS
73	101927	5-23-24	knockout	Human	DTK	CRISPR Deletion Library - Drug targets, kinases, phosphatases†	Bassik 2_DTK	2333	24569	https://www.addgene.org/89359/	Puro/mCherry		2				8.8 X 10^8				Josh Langmade has all frozen aliquots
74	101931	5-30-24	knockout	Human	TMMO	CRISPR Deletion Library - Trafficking, mitochondrial, motility†	Bassik 6_TMMO	2252	23615	https://www.addgene.org/89359/	Puro/mCherry		6				1.2 X 10^9				Josh Langmade has all frozen aliquots
75	101929	5-23-24	knockout	Human	MEPR	CRISPR Deletion Library - Membrane proteins†	Bassik 4_MEPR	1098	12334	https://www.addgene.org/89359/	Puro/mCherry		4				3.7 X 10^8				Josh Langmade has all frozen aliquots
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79	Druggable libraries
80	M928.1	1-26-23	knockout	Human		13 Positive Controls, Best Drug Targets, Human		1302	5464		Puro						8.4 X 10^8				Josh Langmade has all frozen aliquots (4 as of 8/1/24)
81	M928.2	1-26-23	knockout	Human		13 Positive Controls, 2nd Best Drug Targets, Human		2228	9353		Puro						8.6 X 10^8				Josh Langmade has all frozen aliquots (10 as of 8/1/24)
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83	M928.1	2-1-24	knockout	Human		13 Positive Controls, Best Drug Targets, Human		1302	5464		Puro						6.8 X 10^8				Josh Langmade has all frozen aliquots (15 as of 8/1/24)
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