Next-Generation-Sequencing.v1.1.0
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PlatformReads x run: (M) Read length: (paired-end*, Half of data in reads**)Run time: (d) Yield: (Gb) Rate: (Gb/d) Reagents: ($K)per-Gb: ($)hg-30x: ($)Machine: ($)Install baseMax theoretical output per day (Gb)Max total output per day (Gb) from current installbase#PlatformInstall baseMax theoretical output per day (Gb)Max total output per day (Gb) from current installbase#Platformlengthyield
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iSeq 100 1fcell4250*077-1.281.2-21.560.6255216250019.9K101.5615.6--iSeq 100 1fcell10216--iSeq 100 1fcell250*1.2-2
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MiniSeq 1fcell25150*17.57.51.752332800049.5K6007.54500--MiniSeq 1fcell60084500--MiniSeq 1fcell150*7.5
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MiSeq 1fcell25300*2157.5166800099K60007.545000--MiSeq 1fcell6000845000--MiSeq 1fcell300*15
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NextSeq 550 1fcell400150*1.21201005505000250K2400100240000--NextSeq 550 1fcell2400100240000--NextSeq 550 1fcell150*120
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HiSeq 2500 RR 2fcells600100*1.125120106.66.14551.26144740K0106.60--HiSeq 2500 V4 2fcells1500166249000--HiSeq 2500 RR 2fcells100*120
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HiSeq 2500 V3 2fcells3000100*116005523.4739.14692690K0550--HiSeq 4000 2fcells400400160000--HiSeq 2500 V3 2fcells100*600
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HiSeq 2500 V4 2fcells4000125*6100016629.931.73804690K1500166249000--HiSeq X 2fcells400600240000--HiSeq 2500 V4 2fcells125*1000
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HiSeq 4000 2fcells5000150*3.51500400--20.52460900K400400160000--NovaSeq S4 2fcells2851200342000--HiSeq 4000 2fcells150*1500
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HiSeq X 2fcells6000150*31800600--7.08849.61M400600240000--Illumina PacBio RSII1503420--HiSeq X 2fcells150*1800
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NovaSeq S1 2fcells3300150*1.66100060018.7518.751800999K06000--Illumina PacBio Sequel 16cells v6.0 2018275246655--NovaSeq S1 2fcells150*1000
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NovaSeq S2 2fcells6600150*1.66200012003517.51564999K000--MinION Mk 1B 1fcell60001590000--NovaSeq S2 2fcells150*2000
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NovaSeq S4 2fcells20000150*1.83600036006410.67700999K2851200342000--GridION X5 5fcells100757500--NovaSeq S4 2fcells150*6000
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Illumina PacBio RSII0.8820K**4.3122.82.420024000695K1502.8420--PromethION 48fcells455769259615--Illumina PacBio RSII20K**12
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Illumina PacBio Sequel 16cells v6.0 20186.445K**6.6160-32024-48--809600350K27524.26655--MGISEQ T7 4fcells160006000--Illumina PacBio Sequel 16cells v6.0 201845K**160-320
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Illumina PacBio Q1 2019--45K**--192--16.61000350K000--BGISEQ 5007003725900-- Illumina PacBio Q1 201945K**192
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5500 XL14006071803010.558.337000595K000--BGISEQ 505000--5500 XL60180
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Ion S5 510 1chip2 - 3200-4000.2114.80.9595011400065K000--MGISEQ 200010048048000--Ion S5 510 1chip200-4001
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Ion S5 520 1chip3 - 6200-6000.2314.315006000065K000--MIGSEQ 2002030600--Ion S5 520 1chip200-6001
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Ion S5 530 1chip20200-6000.29413.81.21501800065K000----Ion S5 530 1chip200-6004
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Ion S5 540 1chip802000.421535.71.493.31119665k000----Ion S5 540 1chip20015
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Ion S5 550 1chip1302000.525501.6766.8801665k000----Ion S5 550 1chip20025
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SmidgION 1fcell--500-2000000TBCTBCTBCTBCTBC----000----SmidgION 1fcell500-2000000TBC
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Flongle 1fcell--500-200000010.1/1.8-3.3----90-308100-2700--000----Flongle 1fcell500-20000000.1/1.8-3.3
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MinION Mk 1B 1fcell--500-2000000317/30-50----50-12.52700-1125--60001590000----MinION Mk 1B 1fcell500-200000017/30-50
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GridION X5 5fcells--500-2000000385/150-250----47.5/15.70-71575-675--100757500----GridION X5 5fcells500-200000085/150-250
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PromethION 48fcells--500-20000002.63000/7000-15000----14/7-3.51400-315--455769259615----PromethION 48fcells500-20000003000/7000-15000
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QiaGen GeneReader48100-150--7.2--0.5------000----QiaGen GeneReader100-1507.2
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MGISEQ T7 4fcells20000150*1600060004.8??0.8??100??--160006000MGISEQ T7 4fcells150*6000
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BGISEQ 5001600150*726037----600?240K7003725900----BGISEQ 500150*260
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BGISEQ 50160050*0.4820--------5000----BGISEQ 5050*8
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MGISEQ 20003600150*314404807.25600320K10048048000----MGISEQ 2000150*1440
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MIGSEQ 200300100*26030------160K2030600----MIGSEQ 200100*60
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Total (Gb/day)1725205.985
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Human genomes (30x)/day19,169
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Human genomes (30x)/year6,996,669
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Years to sequence 7.6B people1086 yrs
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Number of NovaSeqs or PromethIONs needed to sequence 7.6B people in 10 years156,164
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