Next-Generation-Sequencing.v1.5.4 @albertvilella
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PlatformReads x run: (M) Read length: (paired-end*, Half of data in reads**)Run time: (d) Yield: (Gb) Rate: (Gb/d) Reagents: ($)per-Gb: ($)hg-30x: ($)Machine: ($)Install baseMax theoretical output per day (Gb)Max total output per day (Gb) from current installbase#PlatformInstall baseMax theoretical output per day (Gb)Max total output per day (Gb) from current installbase#Platformlengthyield
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ILMN iSeq 100 1fcell4150*077-1.281.20.796505425416719.9K8601.561341.6--ILMN iSeq 100 1fcell86021342--ILMN iSeq 100 1fcell150*1.2
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ILMN MiniSeq 1fcell25150*17.57.517502182180049.5K11007.58250--ILMN MiniSeq 1fcell110088250--ILMN MiniSeq 1fcell150*7.5
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ILMN MiSeq 1fcell25300*17/01/2020157.517501171170099K74007.555500--ILMN MiSeq 1fcell7400855500--ILMN MiSeq 1fcell300*15
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ILMN NextSeq 550 1fcell400150*1.21201005125434300250K3600100360,000--ILMN NextSeq 550 1fcell3600100360000--ILMN NextSeq 550 1fcell150*120
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ILMN HiSeq 2500 RR 2fcells600100*1.125300106.613920464600740K0106.60--ILMN HiSeq 2500 V4 2fcells1300166215800--ILMN HiSeq 2500 RR 2fcells100*300
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ILMN HiSeq 2500 V3 2fcells3000100*116005535300595900690K0550--ILMN HiSeq 4000 2fcells400400160000--ILMN HiSeq 2500 V3 2fcells100*600
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ILMN HiSeq 2500 V4 2fcells4000125*2.5100016633800343400690K1300166215,800--ILMN HiSeq X 2fcells400600240000--ILMN HiSeq 2500 V4 2fcells125*1000
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ILMN HiSeq 4000 2fcells5000150*3.5150040034450232300900K400400160,000--ILMN NovaSeq S4 2fcells*92036003312000--ILMN HiSeq 4000 2fcells150*1500
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ILMN NextSeq 2000 P3 1fcell (late 2020)1000150*23001506000202000335K01500PACB RSII1003280
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ILMN HiSeq X 2fcells6000150*31800600284401616001M-1.2M400600240,000--PACB 8M Sequel II v2.0 chem 1 flowcell12565081250--ILMN HiSeq X 2fcells150*1800
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ILMN NovaSeq SP 2fcells1600250*1.58800505.311700151500985K06000ONT MinION Mk 1B 1fcell45001567500
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ILMN NovaSeq S1 2fcells3200150*1.66100060014000141400985K06000--ONT GridION X5 5fcells100757500--ILMN NovaSeq S1 2fcells150*1000
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ILMN NovaSeq S2 2fcells6600150*1.662500150632400131300985K000--ONT PromethION 48fcells455769259615--ILMN NovaSeq S2 2fcells150*2500
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ILMN NovaSeq S4 2fcells*20000150*1.8360003600653607.00700985K92036003,312,000--MGI DNBSEQ-T7 4fcells206000--ILMN NovaSeq S4 2fcells*150*6000
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PACB RSII0.8820K**4.3122.8240020024000695K1002.8280--MGI DNBSEQ-G400RS 2fcells1130--PACB RSII20K**12
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PACB 8M Sequel II v2.0 chem 1 flowcell445K-185K**130-18025-650130043.3-7.23897-648350K12565081,250--DNBSEQ-G50 2fcells13030--PACB 8M Sequel II v2.0 chem 1 flowcell45K-185K**30-180
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PACB 48M chip R&D 1 flowcell2445K-185K**1180-1080--13007.2-1.2648-108350K010800--Genapsys 1M Chip13030--PACB 48M chip R&D 1 flowcell45K-185K**180-1080
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TMO SOLID 5500 XL1400607180301050058.337000595K000--Genapsys 16M Chip13030--TMO SOLID 5500 XL60180
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TMO Ion S5 510 1chip2 - 3200-4000.2114.895095011400065K000--Genapsys 144M Chip (2020)13030--TMO Ion S5 510 1chip200-4001
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TMO Ion S5 520 1chip3 - 6200-6000.2314.310005006000065K000----TMO Ion S5 520 1chip200-6001
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TMO Ion S5 530 1chip20200-6000.29413.812001501800065K000----TMO Ion S5 530 1chip200-6004
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TMO Ion Genexus GX5 1chip48-60200-400--9.6-24--3500104.2-364.61119665k000----TMO Ion Genexus GX5 1chip200-4009.6-24
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TMO Ion S5 540 1chip802000.421535.7140093.31119665k000----TMO Ion S5 540 1chip20015
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TMO Ion S5 550 1chip1302000.52550167066.8801665k000----TMO Ion S5 550 1chip20025
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ONT Plate Flongle 1fcell=1well (2020)--500-200000030.5/1--25/50100-25----000
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ONT SmidgION 1fcell--500-2000000--------------000----ONT SmidgION 1fcell500-20000001-2
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ONT Flongle 1fcell--500-20000000.71-21.39090-458100-2700--000----ONT Flongle 1fcell500-2000000#REF!
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ONT MinION Mk 1B 1fcell--500-2000000317/30-50--900-47553-9.54770-885--45001567,500----ONT MinION Mk 1B 1fcell500-200000017/30-50
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ONT GridION X5 5fcells--500-2000000385/150-250--900-47553-9.54770-88550K100757,500----ONT GridION X5 5fcells500-200000085/150-250
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ONT PromethION 48fcells--500-20000002.63000/7000-15000--2000-62520-31400-315285K455769259,615----ONT PromethION 48fcells500-20000003000/7000-15000
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MGI DNBSEQ-Tx 8fcells (2020)230000PE150*3.57000020000700001100--0200000MGI DNBSEQ-Tx 8fcells (2020)PE150*70000
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MGI DNBSEQ-T7 4fcells20000PE150*1600060003500055001M206000
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MGI DNBSEQ-G400RS 2fcells3600PE200*1.61440900--7700360K1130--
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DNBSEQ-G50 FCL 2fcells (2020Q2)500PE150*2.515060------------------DNBSEQ-G50 FCL 2fcells (2020Q2)PE150*150
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DNBSEQ-G50 2fcells300PE100*260302160036043200320K13030----DNBSEQ-G50 2fcellsPE100*60
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Genapsys 1M Chip11501.170.20.2100500600009.9K13030--
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Genapsys 16M Chip10 - 121501.171.2-2.1NA299142170869.9K13030--
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Genapsys 144M Chip (2020)144PE150*1.1730NA5992023969.9K13030--
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Genapsys 144M Chip (2020) long144PE250*250NA5991214389.9K13030--
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QiaGen GeneReader48100-150--7.2--500------000----QiaGen GeneReader100-1507.2
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Total (Gb/day) all sequencers4,769,066.98
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Human genomes (30x)/day52,99090
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Human genomes (30x)/year19,341,216365
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Years to sequence 7700000000 people398 yrs7,700,000,000
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Number of NovaSeqs or PromethIONs or MGIT7s needed to sequence 7700000000, people in 10 years9493 - 5274010
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Number of @illumina NovaSeqs needed to sequence 131M newborns per year (and fold increase of current install base)8,973(9.8x)
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Number of @MGI_BGI T7s needed to sequence 131M newborns per year (and fold increase of current install base)1,615#DIV/0!
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Platformper-Gb: ($)Gigs per $1000 lowerGigs per $1000 higher
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ILMN iSeq 100 1fcell5421.8461538461.846153846
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ILMN MiniSeq 1fcell2184.5871559634.587155963
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ILMN MiSeq 1fcell1178.5470085478.547008547
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ILMN NextSeq 550 1fcell4323.2558139523.25581395
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ILMN HiSeq 2500 RR 2fcells4621.7391304321.73913043
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ILMN HiSeq 2500 V3 2fcells5916.9491525416.94915254
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ILMN HiSeq 2500 V4 2fcells3429.4117647129.41176471
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ILMN HiSeq 4000 2fcells2343.4782608743.47826087
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ILMN NextSeq 2000 P3 1fcell (late 2020)205050
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ILMN HiSeq X 2fcells1662.562.5
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ILMN NovaSeq SP 2fcells1566.6666666766.66666667
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ILMN NovaSeq S1 2fcells1471.4285714371.42857143
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ILMN NovaSeq S2 2fcells1376.9230769276.92307692
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ILMN NovaSeq S4 2fcells*7.00142.8571429142.8571429
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PACB RSII20055
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PACB 8M Sequel II v2.0 chem 1 flowcell43.3-7.2#VALUE!#VALUE!
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PACB 48M chip R&D 1 flowcell7.2-1.2#VALUE!#VALUE!
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TMO SOLID 5500 XL58.3317.1438367917.14383679
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TMO Ion S5 510 1chip9501.0526315791.052631579
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TMO Ion S5 520 1chip50022
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TMO Ion S5 530 1chip1506.6666666676.666666667
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TMO Ion Genexus GX5 1chip104.2-364.6#VALUE!#VALUE!
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TMO Ion S5 540 1chip93.310.7181136110.71811361
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TMO Ion S5 550 1chip66.811.1111111133.33333333
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ONT Plate Flongle 1fcell=1well (2020)100-2510.0040.00
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ONT SmidgION 1fcell--#VALUE!#VALUE!
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ONT Flongle 1fcell90-4511.1122.22
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ONT MinION Mk 1B 1fcell53-9.5#VALUE!#VALUE!
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ONT GridION X5 5fcells53-9.5#VALUE!#VALUE!
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ONT PromethION 48fcells20-330-1230-12
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MGI DNBSEQ-Tx 8fcells (2020)110001000
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MGI DNBSEQ-T7 4fcells5200200
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MGI DNBSEQ-G400RS 2fcells7142.8571429142.8571429
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DNBSEQ-G50 FCL 2fcells (2020Q2)--#VALUE!#VALUE!
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DNBSEQ-G50 2fcells3602.7777777782.777777778
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Genapsys 1M Chip50022
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Genapsys 16M Chip142
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Genapsys 144M Chip (2020)20
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Genapsys 144M Chip (2020) long12
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QiaGen GeneReader--
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Total (Gb/day) all sequencers
94
Human genomes (30x)/day
95
Human genomes (30x)/year
96
Years to sequence 7700000000 people
97
Number of NovaSeqs or PromethIONs or MGIT7s needed to sequence 7700000000, people in 10 years
98
Number of @illumina NovaSeqs needed to sequence 131M newborns per year (and fold increase of current install base)
99
Number of @MGI_BGI T7s needed to sequence 131M newborns per year (and fold increase of current install base)
100
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