OLD_hUGDH Crystal Structures

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S
1	Space Group	Unit Cell Constants(a,b,c,α,β,γ)						PDB ID	Construct	Ligands	Resolution (Å)	UGDH Conformation	ASU Contents	Twinning	Solved By	Crystallization Technique	Cryo	Crystals/ time to appear	Crystallization Conditions (technique,ppt, additive, pH, temp )
2	C 1 2 1	176.98	113.96	97.12	90	116.91	90	4RJT	WT	apo	2.7	open	trimer	No	A. Sidlo	Hanging drop			15% PEG 3350, 0.05 M Sodium Formate, 0.1 M HEPES, pH 7.0, 20 degrees
3	C 1 2 1	178.19	114.07	97.24	90	116.85	90	4QEJ	Del-C	apo	2.65	open	trimer	No	G. Custer	1.0 mm Capillary			5 uL of 10.4 mg/ml enzyme and 200 uL of 16% PEG 3350, 100 mM MgCl2, 100 mM MES pH 6.2, 20 degrees
4	C 1 2 1	180.03	116.93	96.34	90	115.93	90	3TF5	Del-V132, FL	apo	2.3	open	trimer	No	N. Sennett	Hanging drop		Triangle plates, 1 week	12% PEG3350, 0.1 mM piperine, 2% DMSO, 125 mM magnesium acetate, 0.1 M sodium glycine, pH 9.4, 20 degrees
5	C 1 2 1	174.46	113.16	97.14	90	117.23	90		I109V+A136M	apo	1.93	E	trimer	No	B. Pioso	Hanging drop	20% EDG		10% PEG 3350, 0.1 M Na Formate, 0.1 M MES (pH 6.8)
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9	P 1 2₁ 1	89.1	196.67	111.74	90	111.88	90	3PTZ	WT	5 mM UDP-Xyl, 5 mM NAD+	2.5	Horseshoe	Hexamer	No	R. Kadirvelraj	Hanging drop			10% PEG3350, 6% heptanediol, 0.1 M HEPES pH 7.5, 25 degrees
10	P 1 2₁ 1	93.4	194.942	109.508	90	110.713	90		A225L, FL	5 mM UDP-Xyl	2.2	Horseshoe	Hexamer	No	P. Gross (incomplete)	Hanging drop	18% 2:2:1 DMSO:ethylene glycol:glycerol	rhomboid wedges, 2 days	10% PEG-3350, 16% 1,5-pentanediol, 100mM HEPES pH 7.4, 20 degrees
11	P 1 2₁ 1	88.89	196.39	111.02	90	111.94	90		I109V+A136M	5 mM UDP-Xylose	1.9	horseshoe	hexamer	No	B. Pioso	Hanging drop, Seeding	5% EDG		8% PEG 3350, 1.4 M 1,6-hexanediol, 0.1 M HEPES (pH 7.0) Seed conditions: 10 mg/mL I109V, 5 mM UDP-Xylose; 10% PEG 3350, 1.4 M 1,6-hexanediol, 0.1 M HEPES (pH 7.0)
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14	C 1 2 1	403.839	112.676	184.804	90	98.5	90		A104L, FL	3 mM UDP-Glc, 5mM NADH			2 Hexamers	No	A. Sidlo	Hanging drop			16% PEG 3350, 0.2 M Ammonium Sulfate, 0.3M NaCl, 1% 1,2 propanediol, 0.1M Tris pH 8.6, 20 degrees
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17	P 2 2₁ 2₁	93.77	199.8	200.82	90	90	90		A225L, Del-C	UDP-Glc, NADH			Hexamer	No (looks tetragonal)	P. Gross (incomplete)	Hanging drop	25% glycerol		20% PEG-3350, 50mM potassium phosphate, 100mM glycine pH 8.8
18	P 2 2₁ 2₁	94.65	200.05	200.45	90	90	90		Del-C	1 mM UDP-Glc, 1 mM NADH, 200 mM betaine			Hexamer	No	P. Gross (incomplete)	Hanging drop	18% 2:2:1 DMSO:ethylene glycol:glycerol	pitted rectangular prisms, 2 days	27% PEG-3350, 1M betaine, 0.1M glycine pH 9.25, 20 degrees
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21	P 2₁ 2₁ 2₁	111.79	160.64	205.86	90	90	90	3PRJ	WT	5 mM UDP-Xyl, 5 mM NADH	3.1	Horseshoe	Hexamer	No	R. Kadirvelraj	Hanging drop			10% PEG3350, 0.9 M hexanediol, 0.1 M HEPES pH 7, 25 degrees
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24	P 3₁ 2 1	113.5	113.5	480.4	90	90	120		Del-C	1 mM UDP-Glc, 1 mM NADH, 200 mM betaine	3.67		Hexamer	No	P. Gross	Hanging drop	20% 2:2:1 DMSO:ethylene glycol:glycerol	flat trapezoidal plates, 1 day	21% PEG-3350, 0.5M KSCN, 0.2M betain, 100mM β-mercaptoethanol, 100mM glycine pH 9.25, 20 degrees
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27	P 1 2₁ 1	88.66	151.07	88.74	90	107.01	90	4EDF	K94E, FL	5 mM UDP-Glc	2.08	Open, Dimer	4 dimers	YES	N. Sennett	Sitting drop			10% PEG20000, 200 mM magnesium chloride, 100 mM citrate tribasic dihydrate, pH 5.0, 25 degrees
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