Manual QC Symphony Fortessa X50 Stay Puft Marshmallow Man
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ABCDEFGHIJKLMNOPQRSTUVWXYZAAABACADAEAFAGAHAIAJAKALAMANAOAPAQARASATAUAVAWAXAYAZBABBBCBDBEBFBGBHBIBJBKBLBMBNBOBPBQBRBS
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Manual QC
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Symphony Fortessa X50 Stay Puft Marshmallow Man
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Select a tube under you experiment and under Cytometer window, select the Laser tab
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Update everything highlighted below on the most recent date, unless you have custom settings or Flow Core says otherwise.
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Date:8/20/20188/17/20188/16/20188/15/20188/14/20188/13/20188/10/20188/9/20188/8/20188/7/20188/2/20188/1/20187/31/20187/30/20187/27/20187/26/20187/25/20187/24/20187/20/20187/19/20187/17/20187/16/20184/3/20181/26/20181/25/201812/15/201712/7/201712/6/20179/20/20179/5/20177/25/20177/24/20175/12/20173/3/20173/2/20172/24/201710/11/20169/27/20169/27/20169/23/20169/23/20169/22/20169/21/20169/20/20169/20/20169/19/2016
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Time:8:40 AM8:30 AM8:10 AM8:27 AM10:00 AM10:50 AM8:30 AM8:30 AM8:50 AM8:50 AM8:22 AM8:42 AM8:50 AM8:50 AM8:30 AM10:00 AM9:38 AM9:10 AM9:00 AM9:00 AM9:25 AM11.30AM11:30 AM4:30 PM4:30 PM9:10 AM9:05 AM10:21 AM3:00 PM5:00 PM1:00 PM1:00 PM4:50 PM9:00 AM4:30 PM1:45 PM10:29 AM11:19 AM10:00 AM7:00 PM10:35 AM1:20 PM5:00 PM4:57 PM3:00 PM12:40 PM
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LOT#80206802068020680206802068020680206802068020680206802068020680206802068020680296802968029680296802968029670106701067010670106701067010670106701067010670106701066252767701067010670106
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Run by:LanaLanaLanaLanaLanaTomokoTomokoTomokoLanaLanaTomokoLanaClaudiaClaudiaTomoko
Courtney/ Tomoko
AshleyAshley TomokoTomokoTomokoClaudiaClaudiaMike LeeMike LeeClaudiaClaudiaMike LeeMike LeeMike LeeMike LeeMike LeeMike LeeMike LeeMichel DuPageMike LeeSerenaSerenaSerenaMike LeeSerenaSerenaSerenaSerenaMike LeeMike Lee
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Laser Delay (Trigger on FSC)
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Blue0.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.000.00
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Red64.4964.1064.7464.4864.8864.0764.4464.4465.4565.4564.1264.0764.3264.5762.4364.7662.1462.8862.2062.0962.6062.3562.7964.4764.6967.4374.4067.0064.9966.4164.9664.3861.8469.2568.4066.8967.4966.4366.4765.9064.2565.6365.0164.9365.6766.41
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Violet-35.98-35.80-36.27-36.16-36.45-35.87-35.92-35.92-36.19-36.42-35.99-36.12-36.13-36.07-35.11-36.29-35.21-35.27-34.71-34.76-34.98-34.76-34.58-32.57-32.20-33.29-36.99-34.00-33.30-33.48-32.67-32.64-35.91-29.97-29.90-29.29-30.15-30.05-30.05-30.19-29.50-30.13-29.79-30.10-30.25-30.62
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UV-68.90-68.33-69.22-68.86-69.65-68.64-68.74-68.74-69.76-69.76-68.64-68.72-68.98-69.01-66.98-69.30-67.26-67.46-66.38-66.49-67.09-66.74-66.80-64.85-64.13-67.11-74.90-68.00-66.96-67.71-66.16-66.19-64.25-67.52-67.08-65.92-66.92-66.50-66.71-66.12-64.66-65.97-65.11-65.11-66.02-66.58
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violet2-35.98-35.80-36.27-36.16-36.45-35.87-35.92-35.92-36.19-36.42-35.99-36.12-36.13-36.07-35.11-36.29-35.21-35.27-34.71-34.76-34.76-34.76-34.58-32.57-32.20-33.29-37.50-34.00-33.30-33.48-32.67-32.64-35.91-29.97-29.90-29.29-30.15-30.05-30.05-30.19-29.50-30.13-29.79-30.10-30.25-30.62
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YelGreen30.71-30.5930.8230.6430.4430.4630.7330.7431.2131.2130.5530.4530.3630.6329.6530.7929.1729.6029.3529.3129.4829.5629.7631.1631.1432.6935.6232.2530.6031.5130.7530.6931.0037.5436.8036.2935.7835.2735.5734.5933.9634.6634.0833.7534.6534.70
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Area Scaling
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Blue0.970.960.950.950.950.960.950.950.960.960.960.960.970.960.980.950.980.980.980.980.980.980.980.991.000.990.900.980.970.960.970.981.010.981.071.010.990.970.990.970.990.980.990.990.980.97
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Red0.770.770.770.770.770.780.770.770.770.770.770.770.780.770.780.770.780.780.800.800.800.800.800.800.810.770.720.800.770.770.780.780.990.770.790.800.780.790.800.800.810.800.810.810.800.80
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Violet.0.760.760.770.760.780.770.750.750.760.760.740.740.770.770.760.750.790.790.790.780.790.780.730.730.750.690.630.710.720.730.740.740.840.760.780.770.780.800.800.820.830.820.840.840.830.83
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UV0.970.980.970.970.930.970.970.970.970.970.970.970.970.970.990.970.980.97110.9911.001.000.970.970.910.960.980.980.990.991.211.000.951.011.011.001.010.981.021.001.010.980.990.98
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violet20.760.760.770.760.780.770.750.750.760.760.740.740.770.770.760.750.790.790.790.780.780.780.730.730.750.690.630.710.720.730.740.740.840.760.780.770.780.800.800.820.830.820.840.840.830.83
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YelGreen0.930.930.930.930.9330.460.930.930.930.930.930.930.940.930.940.930.950.940.950.950.950.800.950.960.960.940.870.940.940.930.950.941.040.920.930.960.930.940.940.940.950.950.960.960.940.93
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Window Extension
10.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.0010.00
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FSC Area Scaling0.790.800.790.800.800.800.800.800.790.790.800.790.800.790.810.790.810.800.810.810.810.810.800.810.810.790.750.810.800.800.800.810.830.860.860.870.870.870.880.870.880.870.880.880.870.86
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Notes: New configurations for CST and running samples created. Ran new baseline. Red C , UV G, F & E are out of rangeRed C , UV G, F & E are out of rangeUV G, F and E are out of range.UV G, F and E are out of range.UV G, F and E are out of range.
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