SEA-PHAGES FUNCTIONAL ASSIGNMENTS
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7/2/2020 14:22:09
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Last modified:5/30/2020 12:13:02(auto-updated)
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USEDo NOT useNotesExample
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terminase, small subunitTerSSisi_1
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terminaseIf there are not two obvious large and small terminase genes in the same genome, just assign the function "terminase".TM4_4
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terminase, large subunitTerLSisi_2
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terminase, large subunit (ATPase domain) Only applicable to Cluster AY genomes (8-21-18) and AT genomes (2-28-2020). AS genomes appear to have a gene 1 with some alignment to the large subunit, but it is unclear if the domains are intact. (10-21-19, 2-21-2020)Auxilium_gp2
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terminase, large subunit (nuclease domain) Only applicable to Cluster AY genomes (8-21-18) and AT genomes (2-28-2020). AS genomes appear to have a gene 1 with some alignment to the large subunit, but it is unclear if the domains are intact. (10-21-19, 2-21-2020)Auxilium_gp3
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portal proteinhead to tail connectorTM4_5
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portal and MuF-like fusion proteincluster AK arthrobacter phages
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scaffolding proteinScaffoldSisi_5
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capsid maturation proteaseSisi_4
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major capsid proteincapsidSisi_6
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minor capsid proteinother than the MuF minor capsid protein and LamD decoration protein
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MuF-like minor capsid protein
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capsid decoration protein, LamD-likeTuruncu_32
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capsid maturation protease and MuF-like fusion proteinwe are no longer using "capsid morphogenesis protein"Schubert_gp3
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major capsid and protease fusion proteinin this case, the scaffolding function is also part of the fusion, but we don't explicitly write it in the function nameCluster AN arthrobacter phages, EE microbacterium phages
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head fiber proteinBriton15_18
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head-to-tail adaptorwe are no longer calling "head-to-tail connector" or "head-to-tail connector complex protein" 3-6-19must have an HHPRED alignment to one of the following crystal structures: SPP1 15 (5A21 chain C or D in the macromolecular complex) OR must have an HHPRED alignment to one of the following crystal structures: HK97 gp6 or or Bacillus protein yqbG Please see the portal and head-to-tail connector case study at the links provided. Note: SPP1 gp17 and 17.1 are NOT h-t connectors (they are the tail terminator and major tail subunit.https://seaphages.org/meetings/33/
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head-to-tail stoppermust have an HHPRED alignment to one of the following crystal structures: SPP1 16 (5A21 chain E or F in the macromolecular complex)Please see the portal and head-to-tail connector case study at the links provided. Note: SPP1 gp17 and 17.1 are NOT h-t connectors (they are the tail terminator and major tail subunit.https://seaphages.org/meetings/33/
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tail terminatormust have an HHPRED alignment to one of the following: SPP1 17 (5A21 chain G in the macromolecular complex) or Lambda U (3FZ2_chains A through F)Please see the portal and head-to-tail connector case study at the links provided. Note: SPP1 gp17 and 17.1 are NOT h-t connectors (they are the tail terminator and major tail subunit.)https://seaphages.org/meetings/33/
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major tail proteinmajor tail subunitSisi_11
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tail assembly chaperoneTail scaffolding proteinTM4_15; 16
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tape measure proteinTape Measure, tmp, tapemeasureTM4_17
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minor tail proteintail fiber-like protein, collagen-like, glycine richIf you have significant hits to either collagen-like or glycine-rich proteins, and are in the syntenic region of minor tail proteins, you can call them minor tail proteins.Sisi_15-18, Nebs_gp4
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minor tail protein, D-ala-D-ala carboxypeptidasemust include "minor tail" as part of the functional assingmentSisi_19
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tail sheath proteinfound in contractile tailed phagesAlice_120
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tail fiber
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tailspike proteintailspike has triple beta coils. make sure you are matching the spike part of the protein and not the N-terminal tail tip binding domain.Turuncu_23
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tail needle proteinZeta1847_gp20
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baseplate J proteinAlice_133
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tail tube proteinfound in contractile tailed phagesRosiePosie_19
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baseplate wedge proteinRosiePosie_38
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capsid decoration proteinhead decoration proteinmatches LamD, for exampleAlice_107, Alice_232
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lysin ALysA, endolysin Aonly appropriate for Mycobacteriophages; or for Actino phage in which you can identify a lysin bif not a Mycobacteriophage, must have a lysin B, otherwise it is endolysinSisi_30
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lysin A, protease M15 domainsome Gordonia phages have lysin A split into two genes. make sure to label each domain.if not a Mycobacteriophage, must have a lysin B, otherwise it is endolysin, protease M15 domain
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lysin A, protease M23 domainif not a Mycobacteriophage, must have a lysin B, otherwise it is endolysin, protease M23 domain
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lysin A, protease C39 domainsome Gordonia phages have lysin A split into two genes. make sure to label each domain.if not a Mycobacteriophage, must have a lysin B, otherwise it is endolysin, protease C39 domain
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lysin A, glycosyl hydrolase domainsome Gordonia phages have lysin A split into two genes. make sure to label each domain.if not a Mycobacteriophage, must have a lysin B, otherwise it is endolysin, glycosyl hydrolase domain
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lysin A, L-Ala-D-Glu peptidase domainif not a Mycobacteriophage, must have a lysin B, otherwise it is endolysin, L-Ala-D-Glu peptidase domain
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lysin A, N-acetylmuramoyl-L-alanine amidase domain
if not a Mycobacteriophage, must have a lysin B, otherwise it is endolysin, N-acetylmuramoyl-L-alanine amidase domain
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lysin A, protease domainif not obviously M15 or C39some actinobacteriophages (not yet seen in the mycobacteriophages) have lysin A split into two genes. make sure to label each domain.if not a Mycobacteriophage, must have a lysin B, otherwise it is endolysin, protease domain
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lysin BLysB, endolysin BSisi_31
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endolysinsome arthrobacter and streptomyces phages have a single endolysin with domains not found in the Mycobacteriophages (like the CHAP domain). use "endolysin" rather than lysin A if the phage does not infect Mycobacterium and no lysin b can be identified.
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holinmust be adjacent to lysins and have mutliple transmembrane domainsSisi_32
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serine integraseBxb1_35
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tyrosine integraseSisi_43
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tyrosine homologous recombinasefor phages that have an apparent integrase, but none of the rest of the machinery to be temperate is identifiable iin the genome (like an immunity repressor, attP, excise, etc)Settecandela_38
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serine homologous recombinase
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ParA-like dsDNA partitioning proteinRedRock_37
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ParB-like dsDNA partitioning proteinDo not label anything a ParB or having a ParB partitioning domain without the presence of a ParA partner in the genomeRedRock_38
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ParB-like nuclease domaindoes not have to have a ParA partner
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RepA-like replication initiator
Rachaly_36
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immunity repressorRepressorlikely to have an HHPred match to C1 protein in lambdaSisi_45
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exciseExcisionase, Xis, only one per phage;check to see if CRODo not call a protein excise unless you can identify the integrase and the immunity repressor in the phage. A more general "helix-turn-helix DNA binding protein" might be more appropriate if you can't distinguish otherwise.BPs_34
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recombination directionality factorRDFRedRock_58
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Cro (control of repressor’s operator)Do not call a protein Cro unless you can identify the integrase, excise, and the immunity repressor in the phage.Che9c_47
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antirepressorSisi_47
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DnaE-like DNA polymerase III (alpha)Spud_203
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DNA polymerase ILuchador_50
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DNA polymerase III sliding clamp (Beta)Corndog_84
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DnaC-like helicase loaderAlice_189
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DNA helicaseATP-dependent helicaseChah_54
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DnaB-like dsDNA helicaseRedRock_68
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RepA-like helicaseSour_51
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DNA primaseSpud_199
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DNA primase/helicasemake sure it has both partsSchubert_31
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DNA primase/polymerasemake sure it has both partsRosebush_54
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DNA primase/polymerase/helicasemake sure it has all three partsGreenHearts_47
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DnaQ-like (DNA polymerase III subunit)DNAQ is the exonuclease of Pol III (epsilon subunit)Sisi_35
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Nucleotidyl transferaseSpud_3
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polynucleotide kinasepnkSpud_250
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Lsr2-like DNA bridging proteinLsr2Omega_61
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RecA-like DNA recombinaseSpud_205
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RecB-like exonuclease/helicaseIf both a helicase and nuclease domain are present, the RecB label should be used.RedRock_72
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Cas4 family exonucleaseThis family of exonucleases is similar to the exonuclease domain of RecB. The Cas4 label should be used if the gene includes only the exonuclease region. IF the gene also includes a helicase domain, the RecB label should be used. Cas4 family nucleases tend to have alignments to the crystal structure 3H4R_A and to the PD-(D/E)XK nuclease superfamily (PF12705.7, among others).Baby16_68
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Ku-like dsDNA break-binding proteinKuOmega_206
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MRE11 double-strand break endo/exonuclease
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DprA-like DNA processing chain A
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RNA ligaseCjw1_93
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rtcB-like RNA ligaseAnaya_88
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DNA ligaseBenedict_29
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RNaseE
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WhiB family transcription factorWhiBSisi_55,57
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RNA polymerase sigma factorA misinterpretation of crystal structure chains lead to the mis-assignment of Zetzy1847 gene 42 as DNA directed RNA polymerase.Nerujay_52 , Zeta1847 gp42
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ASC-1 transciption coactivator
Zombie_38
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ribonucleotide reductaseRedRock_53
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SprT-like proteasePeaches_79
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cysteine proteaseSaguaro_6
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pyrimidine deaminaseDaVinci_40
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nucleoside deaminasePeaches_35
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deoxycytidylate deaminasedCMP deaminase
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nucleoside deoxyribosyltransferase
EniyanLRS_84
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ThyX-like thymidylate synthaseThyXRedRock_51
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thymidylate synthasewhen you can't determine if it is ThyX or ThyADismas_55
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