RNA Mini Prep Buffers.xlsx

	A	B	C	D	E	F
1	GuSCN Lysis Buffer				Final mL:
2	Component	Stock Conc	Final Conc	Unit	50
3	Guanidinium thiocyanate	118.16	4	g/mol; M	23.632	g GuSCN (s)
4	Tris-HCl, pH 7.5	1000	55	mM	2.75
5	EDTA	500	25	mM	2.5
6	Triton X-100	20	3	%	7.5
7	Water	-	-	-	to final
8
9	Wash Buffer 1				Final mL:
10	Component	Stock Conc	Final Conc	Unit	50
11	Guanidinium thiocyanate	118.16	0.9	g/mol; M	5.3172
12	Ethanol	100	20	%	10
13	Tris-HCl, pH 7.5	1000	10	mM	0.5
14	Water	-	-	-	to final
15
16	Wash Buffer 2				Final mL:
17	Component	Stock Conc	Final Conc	Unit	50
18	NaCl	5000	100	mM	1
19	Ethanol	100	80	%	40
20	Tris-HCl, pH 7.5	1000	10	mM	0.5
21	Water	-	-	-	8.5
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