Restriction Enzymes
 Share
The version of the browser you are using is no longer supported. Please upgrade to a supported browser.Dismiss

 
£
%
123
 
 
 
 
 
 
 
 
 
ABCDEFGHIJKLMNOPQRSTUVWXYZ
1
2
Project Collaborators: Open Bioeconomy Lab (University of Cambridge), Kumasi Hive
Individual Contributors: Jenny Molloy (University of Cambridge), Harry Akligoh (Kumasi Hive), Chiara Gandini (University of Cambridge)
Supported by: Shuttleworth Foundation

This spreadsheet documents REs sequences whereby the patent covering the enzyme or sequence itself has expired.
The uses of that enzyme for molecular biology and biotechnology protocols and applications may still be covered by patents in various countries and we are compiling a list of these separately.
3
4
5
6
7
8
Key to column headings
9
Restriction Enzymes (RE)Name or commonly used description of the REs. Equivalent trademarked names are provided for reference where applicable.
10
Patent ExpiredWhether the patent covering the enzyme or its sequence has expired.
11
Features and ApplicationsFeatures of the polymerase and its applications in molecular biology and biotechnology
12
Original PaperThe paper where the enzyme is initially described, in some cases this is the original discovery and in some cases the first characterisation of the recombinant form or where possible both cases are provided.
13
Original Patent FamilyThe first patent family covering the enzyme, which may not include all subsequent modifications.
14
US Filing/Application DatePriority date for the first US patent covering the enzyme. This is the date at which protection for the invention begins.
15
US Issue DateIssue date for the first US patent covering the enzyme.
16
US Expiration Date
In the United States, for utility patents filed on or after June 8, 1995, the term of the patent is 20 years from the earliest filing date of the application on which the patent was granted and any prior U.S. or Patent Cooperation Treaty (PCT) applications from which the patent claims priority (excluding provisional applications). For patents filed prior to June 8, 1995, the term of patent is either 20 years from the earliest filing date as above or 17 years from the issue date, whichever is longer. We have used this formula except in cases where i) an extension to term or a termination has been specified in the patent; ii) the patent is listed as expired due to lack of payment of fees.
17
OrganismOrganism from which the wild-type sequence was sourced.
18
DNA Sequence
19
Protein Sequence
20
NotesAny additional notes relevant to the enzyme or patents
21
Twist synthesis scoreIs the score that the sequence will reach if submitted to Twist for synthesis. It could be standard, Difficult, Impossible or Too Short (impossible sequences are adjusted to standard e.g. tandem of A are broke changing codon)
22
Internal Project Notes
23
Purification/Affinity Tags Used
24
Notes on Benchling
25
26
27
Useful database is REBASE
28
It is a database that collects restriction enzymes sequences, their isoschizomers and methyltransferases
29
Unless stated, the methyltransferases genes are part of the patent indicated for the companion enzyme
30
31
32
not in the Open Enzyme collection yet
33
present in the open enzyme collection
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
Loading...