Restriction Enzymes

	A	C
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2	Project Collaborators: Open Bioeconomy Lab (University of Cambridge), Kumasi Hive Individual Contributors: Jenny Molloy (University of Cambridge), Harry Akligoh (Kumasi Hive), Chiara Gandini (University of Cambridge) Supported by: Shuttleworth Foundation This spreadsheet documents REs sequences whereby the patent covering the enzyme or sequence itself has expired. The uses of that enzyme for molecular biology and biotechnology protocols and applications may still be covered by patents in various countries and we are compiling a list of these separately.
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7	IMPORTANT: SYNTHESIS STATUS INFORMATION (see colour of cells in Restriction Enzymes sheet)
8	not in the Open Enzyme DNA collection yet
9	present in the Open Enzyme DNA collection
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11	Number of Open Enzymes Synthesised	4
12	Number of Open Enzymes Not Yet Synthesised	20
13	Total Open Enzymes in Collection	24
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15	Key to column headings
16	Restriction Enzymes (RE)	Name or commonly used description of the REs. Equivalent trademarked names are provided for reference where applicable.
17	Patent Expired	Whether the patent covering the enzyme or its sequence has expired.
18	Features and Applications	Features of the polymerase and its applications in molecular biology and biotechnology
19	Original Paper	The paper where the enzyme is initially described, in some cases this is the original discovery and in some cases the first characterisation of the recombinant form or where possible both cases are provided.
20	Original Patent Family	The first patent family covering the enzyme, which may not include all subsequent modifications.
21	US Filing/Application Date	Priority date for the first US patent covering the enzyme. This is the date at which protection for the invention begins.
22	US Issue Date	Issue date for the first US patent covering the enzyme.
23		In the United States, for utility patents filed on or after June 8, 1995, the term of the patent is 20 years from the earliest filing date of the application on which the patent was granted and any prior U.S. or Patent Cooperation Treaty (PCT) applications from which the patent claims priority (excluding provisional applications). For patents filed prior to June 8, 1995, the term of patent is either 20 years from the earliest filing date as above or 17 years from the issue date, whichever is longer. We have used this formula except in cases where i) an extension to term or a termination has been specified in the patent; ii) the patent is listed as expired due to lack of payment of fees.
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28	Organism	Organism from which the wild-type sequence was sourced.
29	DNA Sequence
30	Protein Sequence
31	Notes	Any additional notes relevant to the enzyme or patents
32	Twist synthesis score	Is the score that the sequence will reach if submitted to Twist for synthesis. It could be standard, Difficult, Impossible or Too Short (impossible sequences are adjusted to standard e.g. tandem of A are broke changing codon)
33	Internal Project Notes
34	Purification/Affinity Tags Used
35	Notes on Benchling
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38	Useful database is REBASE
39	It is a database that collects restriction enzymes sequences, their isoschizomers and methyltransferases
40	Unless stated, the methyltransferases genes are part of the patent indicated for the companion enzyme
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