1.1kb Anchor PCR (pRL574 ) to test OpenPCR
 Share
The version of the browser you are using is no longer supported. Please upgrade to a supported browser.Dismiss

 
View only
 
 
Still loading...
ABCDEFGHIJKLMNOPQRST
1
Final AmountsMasterMix12345
2
1.5ng/ul pRL574.3ng/ul5.2511111
3
10uM R19850.5uM13.1252.52.52.52.52.5
4
10uM F834D0.5uM13.1252.52.52.52.52.5
5
10x Buffer1x26.2555555
6
50mM MgCl21.5mM7.8751.51.51.51.51.5
7
10mM dNTP.2mM5.2511111
8
5U/ul Taq 1U1.050.20.20.20.20.2
9
H2Ofill to100ul190.57536.336.336.336.336.3
10
Total100ul total262.55050505050
11
12
13
ProgrampRL1Tin Ant directory
14
Stage 192C3min1 cycle
15
Stage 292C30sec
16
55C30sec
17
72C1min20sec35cycle
18
Stage 372C5min1 cycle
19
12Chold
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
Loading...
 
 
 
Sheet1