Gibson assembly

	A	B	C	D	E
1	5x Isothermal Reaction Mix
2	Reagent	Concentration	Unit	Volume stock / mass (ul)
3	Tris-HCL	500	mM	1000
4	MgCl2	50	mM	50
5	PEG-8000	25	%	625	g
6	NAD	5	mM	200	Total desired volume (ml)
7	DTT	50	mM	1
8	dNTPs	1	mM	20	2
9	PCR grade H20	NA	NA	104
10			Grand total (ml)	2
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12	1.33x Gibson assembly master mix
13	Reagent	Concentration	Unit	Volume stock (ul)
14	Taq ligase	5.3	units/ul	10.6
15	5x isothermal buffer	1	X	16	Total desired volume (ul)
16	DNA polymerase	0.03	units/ul	0.48	80
17	T5 exonuclease	0.005	units/ul	0.4	Total Desired volume (ml)
18	PCR grade H20	NA	NA	52.52	0.08
19			Grand Total	80
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24	Recipes taken from
25	https://openwetware.org/wiki/Gibson_Assembly
26	https://www.protocols.io/view/homemade-gibson-mastermix-n9xdh7n?step=1
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