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Corresponding author
email addressTitleDescription
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link to draft
checked by Bioconductor editors
submitted to F1000Research by author
ready for typesetting
ready for publishing
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John Smith
johnsmith@f1000.com
title of tool/recipexxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxJohn DoeJane DoeBill DoeWeir DoeAmy Doe(google doc)YES/NOYES/NOYES/NOYES/NO
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Aaron Lun
alun@wehi.edu.au
Infrastructure for genomic interactions: Bioconductor classes for Hi-C, ChIA-PET and related experiments
The study of genomic interactions has been greatly facilitated by techniques such as chromatin conformation capture with high-throughput sequencing (Hi-C). These genome-wide experiments generate large amounts of data that require careful analysis to obtain useful biological conclusions. However, development of the appropriate software tools is hindered by the lack of basic infrastructure to represent and manipulate genomic interaction data. Here, we present the InteractionSet package that provides classes to represent genomic interactions and store their associated experimental data, along with the methods required for low-level manipulation and processing of those classes. The InteractionSet package exploits existing infrastructure in the open-source Bioconductor project, while in turn being used by Bioconductor packages designed for higher-level analyses. For new packages, use of the functionality in InteractionSet will simplify development, allow access to more features and improve interoperability between packages.
Jonathan Cairns
Aleksandra Pekowska
Csilla VarnaiNicholas Servant
Douglas Phanstiel
https://github.com/LTLA/BiocISet2016
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Gordon Smyth
smyth@wehi.edu.au
From reads to results: detecting differentially expressed genes in RNA-Seq experiments using Rsubread and the edgeR quasi-likelihood pipeline
This article describes a computational workflow for the detection of DE genes and pathways from RNA-seq data. The workflow is based primarily on R software packages from the open-source Bioconductor project and covers all steps of the analysis pipeline, from alignment of read sequences to interpretation and visualization of analysis results. In particular, the read alignment and count quantification will be conducted using the Rsubread package and the statistical analyses will be performed using the edgeR package. Analyses will be demonstrated on real mouse mammary gland RNA-Seq data. This will provide readers with practical usage examples that can be applied in their own studies.
Steve Lianoglou <lianoglou.steve@gene.com>
Ryan Thompson <rct@thompsonclan.org>
https://www.overleaf.com/5327268zgbttr
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Charity Law
law@wehi.edu.au
RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeRA workflow of an RNA-seq analysis using three Bioconductor packages - limma, Glimma and edgeR.
https://www.overleaf.com/4764408yqfzfm#/16959350/
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