PCR Reactions

	A	B	C	D	E	F	G	H	I	J	K
1	Sample	Polymerase	Fwd Primer	Rev Primer	Template	Template Prep date	Template Volume	Sample Volume	Annealing temp	Cycles	Extension (s)
2	1	PhU	pFC9BisP3Fwd	pFC9BisP4Rev	pFC9 Denatureing Bisulfite	1/19/22	1	25	50	40	15
3	2	PhU	pFC9BisP3Fwd	pFC9BisP4Rev	pFC9 Denatureing Bisulfite	1/19/22	1	25	48.1	40	15
4	3	PhU	pFC9BisP3Fwd	pFC9BisP4Rev	pFC9 Denatureing Bisulfite	1/19/22	1	25	43.3	40	15
5	4	PhU	pFC9BisP3Fwd	pFC9BisP4Rev	pFC9 Denatureing Bisulfite	1/19/22	1	25	40.9	40	15
6	5	PhU	pFC9BisP3Fwd	pFC9BisP4Rev	pFC9 Denatureing Bisulfite	1/19/22	0	25	40	40	15
7
8
9	Master Mix
10	Total Volume	5x HF buffer	10 uM Fwd Primer	10 uM Rev Primer	dNTP (10 mM)	PhusionU Pol
11	125	25	6.25	6.25	1.25	1.25
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