20150319 - qPCR WS cDNA test
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ABCDEFGHIJKLMNOPQRST
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Master Mix Calcs
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CiCfVol. (uL)# RxnsTotal Vol (uL)
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Template1
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Promega 2x GoTaq Probe Master Mix2112.511137.5
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WSN1 F100.320.8118.8
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WSN1 R100.320.8118.8
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RLP_P (ABI)100.20.5115.5
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BSA200.600.75118.25
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H2O8.651195.15
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Total25Add 24uL to each well
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CiCfVol. (uL)# RxnsTotal Vol (uL)
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Template1
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Promega 2x GoTaq qPCR Master Mix2112.520250
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EUB A100.320.82016
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EUB B100.320.82016
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BSA200.600.752015
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H2O9.1520183
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Total25Add 24uL to each well
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