ABCDEFGHIJKLMNOPQRSTUVWXYZ
1
Standard #Total ul needed for qPCR + making next Stdul of the previous Std #ul of MQFactors
2
1--V of template/qPCR reaction, ul5
3
Std #1 diluted by the dilution factor
2222.219.8Number of tech reps3
4
Std #2 diluted by the dilution factor
3222.219.8
Tech error for serial dilution pipetting, ul
5
5
Std #3 diluted by the dilution factor
4222.219.8Dilution factor10
6
Std #4 diluted by the dilution factor
5222.219.8
7
Std #5 diluted by the dilution factor
6222.219.8
8
Std #6 diluted by the dilution factor
720218
9
8NTC
10
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100