Anti-ECM antibodies_Rickelt et al.xlsx

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O
1	Table adapted from Rickelt S and Hynes RO, Matrix Biol, 2018
2	https://doi.org/10.1016/j.matbio.2018.04.011
3	Notes:
4	Antibodies to extracellular matrix proteins tested on formaldehyde-fixed and paraffin-embedded (FFPE) tissue samples using immunohistochemistry (IHC). This table provides an overview of all tested antibodies that showed positive staining signals. Presented are our IHC validation information and results, however, we have not validated the molecular specificity of every antibody (see text).
5	Antibody host species (gt, goat; m, mouse monoclonal antibody; rb, rabbit; rbM, rabbit monoclonal antibody; rt, rat; sh, sheep).
6	Heat-induced epitope retrieval (HIER) method used (0.05% Tween in Citric acid, pH 6.0 or Tris, pH 9.0) or protease treatment (Tris, pH 9.0 + Pepsin treatment); for details see Supplementary Materials and Methods. Individual antibody dilutions recommended, the human and mouse reactivity as well as a positive control tissue, additional notes and references are listed.
7	Additional abbreviations used: CRC, colorectal cancer; NT, not tested; PDAC, pancreatic ductal adenocarcinoma; TEB terminal end buds.
8	* Indicates the preferred HIER method ECM-affiliatedplied for the individual antibody, however, other conditions also might work.
9	** Indicates the antibody dilution we have tested; however, each antibody vial should be tested for individual ECM-affiliatedplication and tissue since lot-specific variability can occur. For additional details on the individual antibodies see also the manufacturers datasheets.
10	‡ A representative image of the staining with the antibody is shown in the Supplementary Figure Gallery.
11
12	Protein name	Gene Symbol	Matrisome Division	Matrisome Category	Host	Company	Catalog number	URL	HIER method *	Dilution **	Human reactivity	Mouse reactivity	Positive control tissue	Additional notes	Suppl. Figure Gallery page #; also available at https://doi.org/10.1016/j.matbio.2018.04.011
13	Agrin	AGRN	Core Matrisome	ECM Glycoproteins	rb	Novus Biologicals	NBP1-90209	https://www.novusbio.com/products/agrin-antibody_nbp1-90209	Tris, pH9.0 + Pepsin	1:400	YES ‡	YES ‡	kidney, lung		4, 5
14	Agrin	AGRN	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab85174	https://www.abcam.com/products/primary-antibodies/agrin-antibody-ab85174.html	Tris, pH9.0 + Pepsin	1:1000-1:2000	YES ‡	YES ‡	kidney, lung		6, 7
15	Agrin	AGRN	Core Matrisome	ECM Glycoproteins	gt	R&D Systems	AF550	https://www.rndsystems.com/products/rat-agrin-antibody_af550	Tris, pH9.0 + Pepsin	1:250-1:500	occasional basal lamina stain	YES ‡	kidney, lung	works on frozen tissues	10
16	Cartilage oligomeric matrix protein	COMP	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab74524	https://www.abcam.com/products/primary-antibodies/compcartilage-oligomeric-matrix-protein-antibody-ab74524.html?productWallTab=ShowAll	Tris, pH9.0	1:250	YES ‡	YES ‡	cartilage, CRC, PDAC		18, 19
17	Cartilage oligomeric matrix protein	COMP	Core Matrisome	ECM Glycoproteins	rt	Abcam	ab11056	https://www.abcam.com/products/primary-antibodies/compcartilage-oligomeric-matrix-protein-antibody-ma37c94-hc484d1-ab11056.html?productWallTab=ShowAll	Tris, pH9.0	1:10	YES ‡	NO	cartilage, CRC, PDAC		20
18	Dermatopontin	DPT	Core Matrisome	ECM Glycoproteins	rb	ABclonal	A8643	https://abclonal.com/catalog-antibodies/DPTRabbitpAb/A8643	Citric, pH6.0	1:1000-1:2000	YES ‡	NT	liver, placental cord		41
19	EGF-containing fibulin-like extracellular matrix protein 1	EFEMP1	Core Matrisome	ECM Glycoproteins	rb	Thermo Fisher Scientific	PA5-29347	https://www.thermofisher.com/antibody/product/Fibulin-3-Antibody-Polyclonal/PA5-29347	Tris, pH9.0	1:250	YES ‡	NT	kidney, lung		42
20	EGF-containing fibulin-like extracellular matrix protein 2	EFEMP2	Core Matrisome	ECM Glycoproteins	rbM	Abcam	ab125073	https://www.abcam.com/products/primary-antibodies/fibulin-4-antibody-epr6842-ab125073.html?productWallTab=ShowAll	Tris, pH9.0	1:500	YES ‡	NO	colon, pancreas		43
21	Elastin	ELN	Core Matrisome	ECM Glycoproteins	m	Abcam	ab77804	https://www.abcam.com/products/primary-antibodies/elastin-antibody-10b8-ab77804.html?productWallTab=ShowAll	Citric, pH6.0	1:250-1:500	YES ‡	NT	colon		44
22	Elastin	ELN	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab21610	https://www.abcam.com/products/primary-antibodies/elastin-antibody-ab21610.html?productWallTab=ShowAll	Citric, pH6.0	1:250-1:500	YES	NT	colon
23	Elastin microfibril interfacer 1	EMILIN1	Core Matrisome	ECM Glycoproteins	rb	Sigma-Aldrich	HPA002822	https://www.sigmaaldrich.com/US/en/product/sigma/hpa002822	Tris, pH9.0	1:500-1:1000	YES ‡	NO	endometrium, intestine		45
24	Fibulin 1	FBLN1	Core Matrisome	ECM Glycoproteins	m	Abcam	ab211536	https://www.abcam.com/products/primary-antibodies/fibulin-1-antibody-cl0337-ab211536.html?productWallTab=ShowAll	Tris, pH9.0	1:50-1:75	YES - but weak ‡	NO	CRC, lung		65
25	Fibulin 2	FBLN2	Core Matrisome	ECM Glycoproteins	rb	Novus Biologicals	NBP1-33479	https://www.novusbio.com/products/fibulin-2-antibody_nbp1-33479	Tris, pH9.0	1:500	YES ‡	NO	lung		66
26	Fibulin 5	FBLN5	Core Matrisome	ECM Glycoproteins	rbM	Abcam	ab109428	https://www.abcam.com/products/primary-antibodies/fibulin-5-antibody-epr4506-ab109428.html?productWallTab=ShowAll	Tris, pH9.0	1:750	YES ‡	YES ‡	intestine, lung		67, 68
27	Fibronectin 1	FN1	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab2413	https://www.abcam.com/products/primary-antibodies/fibronectin-antibody-ab2413.html?productWallTab=ShowAll	Tris, pH9.0	1:10000	YES ‡	YES ‡	CRC, placenta		52, 53
28	Fibronectin 1 - EIIIA/ED1	FN1	Core Matrisome	ECM Glycoproteins	m	Abcam	ab6328	https://www.abcam.com/products/primary-antibodies/fibronectin-antibody-ist-9-bsa-and-azide-free-ab6328.html?productWallTab=ShowAll	Tris, pH9.0	1:100	YES ‡	NO - unclear due to background stain	placenta / CRC		56
29	Fibronectin 1 - EIIIA/ED1	FN1	Core Matrisome	ECM Glycoproteins	m	Sigma-Aldrich	F6140	https://www.sigmaaldrich.com/US/en/product/sigma/f6140	Tris, pH9.0	1:400	YES ‡	NT	placenta / CRC		57
30	Laminin (pan)	-	Core Matrisome	ECM Glycoproteins	rb	Novus Biologicals	NB300-144	https://www.novusbio.com/products/laminin-antibody_nb300-144	Tris, pH9.0	1:500	YES ‡	YES ‡	CRC, intestine, pancreas		77, 78
31	Laminin (pan)	-	Core Matrisome	ECM Glycoproteins	rb	Sigma-Aldrich	L9393	https://www.sigmaaldrich.com/US/en/product/sigma/l9393	Tris, pH9.0	1:100-1:200	NT	YES ‡	CRC, intestine, pancreas		79
32	Laminin (pan)	-	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab11575	https://www.abcam.com/products/primary-antibodies/laminin-antibody-ab11575.html?productWallTab=ShowAll	Tris, pH9.0	1:1000-1:2000	NT	YES ‡	CRC, intestine, pancreas		80
33	Laminin, α1	LAMA1	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91117	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lama1-antibody-amab91117/?q=&t=	Citric, pH6.0	1:250	YES - but weak ‡	NO	CRC, PDAC		81
34	Laminin, α2	LAMA2	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91166	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lama2-antibody-amab91166/?q=&t=	Tris, pH9.0	1:100	YES	NO	CRC, PDAC, placenta
35	Laminin, α2	LAMA2	Core Matrisome	ECM Glycoproteins	m	Novus Biologicals	H00003908-M01	https://www.novusbio.com/products/laminin-alpha-2-antibody-2d4_h00003908-m01	Tris, pH9.0	1:750-1:1000	YES ‡	NO	CRC, PDAC, placenta		82
36	Laminin, α3	LAMA3	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91123	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lama3-antibody-amab91123/?q=&t=	Citric, pH6.0	1:250	YES ‡	NO	CRC, PDAC		83
37	Laminin, α4	LAMA4	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91133	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lama4-antibody-amab91133/?q=&t=	Tris, pH9.0	1:250-1:500	YES ‡	NO	CRC, PDAC		84
38	Laminin, α5	LAMA5	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91124	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lama5-antibody-amab91124/?q=&t=	Tris, pH9.0	1:250-1:500	YES ‡	NO	CRC, PDAC		85
39	Laminin, β1	LAMB1	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91092	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lamb1-antibody-amab91092/?q=&t=	Citric, pH6.0	1:100-1:200	YES ‡	NO	CRC, PDAC		86
40	Laminin, β2	LAMB2	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91096	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lamb2-antibody-amab91096/?q=&t=	Tris, pH9.0	1:1500	YES ‡	NO	CRC, PDAC		87
41	Laminin, β3	LAMB3	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91161	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lamb3-antibody-amab91161/?q=&t=	Tris, pH9.0	1:250	YES ‡	NO	CRC, placental membrane		88
42	Laminin, γ1	LAMC1	Core Matrisome	ECM Glycoproteins	m	Atlas Antibodies	AMAb91137	https://www.atlasantibodies.com/products/antibodies/primary-antibodies/precisa-monoclonals/lamc1-antibody-amab91137/?q=&t=	Citric, pH6.0	1:100	YES ‡	NT	CRC, PDAC		89
43	Latent transforming growth factor β binding protein 1	LTBP1	Core Matrisome	ECM Glycoproteins	m	R&D Systems	MAB388	https://www.rndsystems.com/products/human-latent-tgf-beta-bp1-antibody-35409_mab388	Tris, pH9.0 + Pepsin	1:20	YES ‡	NT	lung		92
44	Latent transforming growth factor β binding protein 2	LTBP2	Core Matrisome	ECM Glycoproteins	rb	Novus Biologicals	NBP1-88411	https://www.novusbio.com/products/latent-tgf-beta-bp2-ltbp-2-antibody_nbp1-88411	Tris, pH9.0	1:200	YES ‡	YES	CRC, lung		94
45	Latent transforming growth factor β binding protein 2	LTBP2	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab121193	https://www.abcam.com/products/primary-antibodies/ltbp2-antibody-ab121193.html?productWallTab=ShowAll	Tris, pH9.0	1:100-1:200	YES	NT	CRC, lung
46	Milk fat globule-EGF factor 8 protein	MFGE8	Core Matrisome	ECM Glycoproteins	m	R&D Systems	MAB27671	https://www.rndsystems.com/search?keywords=+MAB27671	Citric, pH6.0	1:250-1:500	YES ‡	NT	endometrium, pancreas		109
47	Nidogen 2	NID2	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab14513	https://www.abcam.com/products/primary-antibodies/nidogen-2nid-2-antibody-ab14513.html?productWallTab=ShowAll	Tris, pH9.0	1:2000-1:4000	NT	YES ‡	mouse mammary tissue, TEB		115
48	Periostin	POSTN	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab14041	https://www.abcam.com/products/primary-antibodies/periostin-antibody-ab14041.html?productWallTab=ShowAll	Tris, pH9.0	1:500-1:1000	YES ‡	YES ‡	CRC, intestine		116, 117
49	Secreted protein, acidic, cysteine-rich (osteonectin)	SPARC	Core Matrisome	ECM Glycoproteins	rbM	Cell Signaling	8725	https://www.cellsignal.com/products/primary-antibodies/sparc-d10f10-rabbit-mab/8725	Citric, pH6.0	1:100	YES ‡	NT	cartilage, CRC, endometrium	the indivudual antibodies show varying staining pattern	131
50	Secreted protein, acidic, cysteine-rich (osteonectin)	SPARC	Core Matrisome	ECM Glycoproteins	rb	Sino Biological	10929-RP02	https://www.sinobiological.com/antibodies/human-sparc-10929-rp02	Tris, pH9.0	1:500	YES ‡	NT	cartilage, CRC, endometrium	the indivudual antibodies show varying staining pattern	131
51	Secreted protein, acidic, cysteine-rich (osteonectin)	SPARC	Core Matrisome	ECM Glycoproteins	gt	R&D Systems	AF942	https://www.rndsystems.com/products/mouse-sparc-antibody_af942	Tris, pH9.0	1:200	NO	YES	cartilage, CRC, endometrium	the indivudual antibodies show varying staining pattern
52	Secreted phosphoprotein 1 (osteopontin)	SPP1	Core Matrisome	ECM Glycoproteins	m	Abcam	ab166709	https://www.abcam.com/products/primary-antibodies/osteopontin-antibody-7c5h12-ab166709.html?productWallTab=ShowAll	Tris, pH9.0	1:200	YES ‡	NT	bone, cartilage, CRC, kidney		133
53	secreted phosphoprotein 1 (osteopontin)	SPP1	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab8448	https://www.abcam.com/products/primary-antibodies/osteopontin-antibody-ab8448.html?productWallTab=ShowAll	Citric, pH6.0	1:1000-1:2000	YES ‡	NT	bone, cartilage, CRC, kidney		134
54	Sushi-repeat-containing protein, X-linked 2	SRPX2	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab91584	https://www.abcam.com/products/primary-antibodies/srpx2-antibody-ab91584.html?productWallTab=ShowAll	Tris, pH9.0	1:5000	YES ‡	YES	endometrium, placenta		138
55	Transforming growth factor, β-induced, 68kDa	TGFBI	Core Matrisome	ECM Glycoproteins	rb	Sigma-Aldrich	HPA017019	https://www.sigmaaldrich.com/US/en/product/sigma/hpa017019	Tris, pH9.0	1:250:1-500	YES ‡	NT	CRC, placenta		150, 151
56	Thrombospondin 2	THBS2	Core Matrisome	ECM Glycoproteins	m	BD Biosciences	611150	https://www.bdbiosciences.com/en-us/products/reagents/microscopy-imaging-reagents/immunofluorescence-reagents/purified-mouse-anti-mouse-thrombospondin-2.611150	Tris, pH9.0	1:200-1:500	YES - matrix and epithelial cells	NT	CRC, PDAC
57	Thrombospondin 2	THBS2	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab112543	https://www.abcam.com/products/primary-antibodies/thrombospondin-2-antibody-ab112543.html?productWallTab=ShowAll	Tris, pH9.0	1:100-1:200	YES	NT	CRC, PDAC
58	Thrombospondin 2	THBS2	Core Matrisome	ECM Glycoproteins	rb	Thermo Fisher Scientific	PA5-50843	https://www.thermofisher.com/antibody/product/Thrombospondin-2-Antibody-Polyclonal/PA5-50843	Tris, pH9.0	1:500	YES ‡	YES ‡	CRC, PDAC		144, 145
59	Tenascin C	TNC	Core Matrisome	ECM Glycoproteins	rbM	Abcam	ab108930	https://www.abcam.com/products/primary-antibodies/tenascin-c-antibody-epr4219-ab108930.html?productWallTab=ShowAll	Tris, pH9.0	1:1500	YES ‡	YES ‡	CRC, placenta		139, 140
60	Tenascin C	TNC	Core Matrisome	ECM Glycoproteins	rt	Sigma-Aldrich	T3413	https://www.sigmaaldrich.com/US/en/product/sigma/t3413	Tris, pH9.0	1:250-1:500	NO	YES ‡	mouse tumor tissues	specific for mouse tissues	141
61	Tenascin N	TNN	Core Matrisome	ECM Glycoproteins	rb	Abcam	ab121887	https://www.abcam.com/products/primary-antibodies/tenascin-n-antibody-ab121887.html?productWallTab=ShowAll	Tris, pH9.0	1:250	YES ‡	NT	skin		142
62	Tenascin X	TNX	Core Matrisome	ECM Glycoproteins	sh	R&D Systems	AF6999	https://www.rndsystems.com/products/human-tenascin-x-antibody_af6999	Tris, pH9.0	1:100	YES - but weak	NT	CRC, lung mesothelioma
63	Collagen, type I (pan)	-	Core Matrisome	Collagens	m	Abcam	ab88147	https://www.abcam.com/products/primary-antibodies/collagen-i-antibody-3g3-bsa-and-azide-free-ab88147.html?productWallTab=ShowAll	Tris, pH9.0	1:500	YES ‡	NO	intestine, liver, lung		25
64	Collagen, type I (pan)	-	Core Matrisome	Collagens	rb	Abcam	ab21286	https://www.abcam.com/products/primary-antibodies/collagen-i-antibody-ab21286.html?productWallTab=ShowAll	Tris, pH9.0	1:2000	NT	YES ‡	intestine, liver, lung		26
65	Collagen, type I (pan)	-	Core Matrisome	Collagens	rb	MilliporeSigma	AB765P	https://www.sigmaaldrich.com/US/en/product/mm/ab765p	Tris, pH9.0	1:100-1:250	NO	YES - but weak	mouse intestine, lung
66	Collagen, type IV (pan)	-	Core Matrisome	Collagens	rb	Abcam	ab6586	https://www.abcam.com/products/primary-antibodies/collagen-iv-antibody-ab6586.html?productWallTab=ShowAll	Tris, pH9.0 + Pepsin	1:2000-1:4000	YES ‡	YES ‡	most tissues		29, 30
67	Collagen, type VI (pan)	-	Core Matrisome	Collagens	rb	MilliporeSigma	AB7821	https://www.sigmaaldrich.com/US/en/product/mm/ab7821	Tris, pH9.0	1:2000-1:4000	YES ‡	YES ‡	kidney, intestine, ovary		31, 32
68	Endostatin (Collagen XVIII fragment)	-	Core Matrisome	Collagens	gt	R&D Systems	AF1098	https://www.rndsystems.com/products/human-endostatin-antibody_af1098	Tris, pH9.0 + Pepsin	1:300-1:500	YES ‡	NT	CRC, endometrium		48
69	Collagen, type XIII, α1	COL13A1	Core Matrisome	Collagens	rb	Novus Biologicals	NBP2-13854	https://www.novusbio.com/products/collagen-xiii-alpha-1-antibody_nbp2-13854	Tris, pH9.0	1:250	YES ‡	YES ‡	mammary tissue, skin		34, 35
70	Collagen, type I, α1	COL1A1	Core Matrisome	Collagens	sh	R&D Systems	AF6220	https://www.rndsystems.com/products/human-pro-collagen-i-alpha1-antibody_af6220	Tris, pH9.0	1:75	YES ‡	YES - but weak	endometrium, PDAC		27
71	Collagen, type I, α1	COL1A1	Core Matrisome	Collagens	rb	Rockland Immunochemicals	600-401-D19	https://www.rockland.com/categories/primary-antibodies/collagen-i-alpha-1-propeptide-antibody-600-401-D19/	Tris, pH9.0 + Pepsin	1:250	YES	NO	endometrium, PDAC
72	Collagen, type XXII, α1	COL22A1	Core Matrisome	Collagens	rb	Abcam	ab121846	https://www.abcam.com/products/primary-antibodies/col22a1-antibody-ab121846.html?productWallTab=ShowAll	Tris, pH9.0	1:200	NO	YES ‡	mouse muscle-tendon junction		36
73	Collagen, type III, α1	COL3A1	Core Matrisome	Collagens	rb	Abcam	ab7778	https://www.abcam.com/products/primary-antibodies/collagen-iii-antibody-ab7778.html?productWallTab=ShowAll	Tris, pH9.0 + Pepsin	1:500-1:1000	YES ‡	NT	endometrium, skin		28
74	Collagen, type VII, α1	COL7A1	Core Matrisome	Collagens	rb	Abcam	ab93350	https://www.abcam.com/products/primary-antibodies/collagen-vii-antibody-bsa-and-azide-free-ab93350.html?productWallTab=ShowAll	Tris, pH9.0 + Pepsin	1:250	YES ‡	NO	skin		33
75	Heparan sulfate proteoglycan 2 (perlecan)	HSPG2	Core Matrisome	Proteoglycans	rb	Boster	PB9277	https://www.bosterbio.com/anti-hspg2-picoband-trade-antibody-pb9277-boster.html	Tris, pH9.0	1:500-1:750	YES ‡	YES ‡	CRC, intestine, kidney		73, 74
76	Versican	VCAN	Core Matrisome	Proteoglycans	rb	MilliporeSigma	AB1033	https://www.sigmaaldrich.com/US/en/product/mm/ab1033	Citric, pH6.0	1:250	NO	YES ‡	mouse cartilage, TEB	specific for mouse tissues	152
77	Chondroitin sulfate	-	Matrisome Associated	ECM-affiliated	m	Sigma-Aldrich	C8035	https://www.sigmaaldrich.com/US/en/product/sigma/c8035	Citric, pH6.0	1:1000	YES ‡	YES - but weak	placenta		23
78	Annexin A5	ANXA5	Matrisome Associated	ECM-affiliated	rbM	Abcam	ab108321	https://www.abcam.com/products/primary-antibodies/annexin-vanxa5-antibody-epr3979-ab108321.html?productWallTab=ShowAll	Tris, pH9.0	1:200-1:400	YES ‡	NO	colon, CRC		12
79	Annexin A5	ANXA5	Matrisome Associated	ECM-affiliated	rb	Abcam	ab14196	https://www.abcam.com/products/primary-antibodies/annexin-vanxa5-antibody-ab14196.html?productWallTab=ShowAll	Tris, pH9.0	1:1000	YES	NT	colon, CRC
80	Annexin A5	ANXA5	Matrisome Associated	ECM-affiliated	rb	Boster	PA1008	https://www.bosterbio.com/anti-annexin-v-antibody-pa1008-boster.html	Tris, pH9.0	1:200	YES	NT	colon, CRC
81	Complement component C1qA	C1QA	Matrisome Associated	ECM-affiliated	rbM	Abcam	ab189922	https://www.abcam.com/products/primary-antibodies/c1qa-antibody-epr14634-ab189922.html?productWallTab=ShowAll	Tris, pH9.0	1:200-1:400	YES ‡	NT	CRC, kidney		37
82	C1q and TNF related protein 5	C1QTNF5	Matrisome Associated	ECM-affiliated	rb	Abcam	ab36893	https://www.abcam.com/products/primary-antibodies/ctrp5-antibody-ab36893.html?productWallTab=ShowAll	Tris, pH9.0	1:1500	YES ‡	NT	colon, pancreas		16
83	Chondroitin sulfate proteoglycan 4 (NG2)	CSPG4	Matrisome Associated	ECM-affiliated	rb	MilliporeSigma	AB5320	https://www.sigmaaldrich.com/US/en/product/mm/ab5320	Tris, pH9.0	1:50-1:100	YES ‡	NT	CRC		24
84	Endomucin	EMCN	Matrisome Associated	ECM-affiliated	rt	Invitrogen	14-5851	https://www.thermofisher.com/antibody/product/Endomucin-Antibody-clone-eBioV-7C7-V-7C7-Monoclonal/14-5851-82	Tris, pH9.0	1:25	YES - but weak ‡	YES ‡	kidney, lung		46, 47
85	Glypican 1	GPC1	Matrisome Associated	ECM-affiliated	m	MilliporeSigma	MAB2600	https://www.sigmaaldrich.com/US/en/product/mm/mab2600	Tris, pH9.0	1:100	YES ‡	NO	CRC, PDAC		69
86	Glypican 4	GPC4	Matrisome Associated	ECM-affiliated	rb	Abcam	ab118911	https://www.abcam.com/products/primary-antibodies/glypican-4-antibody-ab118911.html?productWallTab=ShowAll	Tris, pH9.0	1:250	YES ‡	YES	kidney, pancreas		70
87	Hemopexin	HPX	Matrisome Associated	ECM-affiliated	m	R&D Systems	MAB4490	https://www.rndsystems.com/products/human-hemopexin-antibody-698813_mab4490	Tris, pH9.0	1:1000-1:2000	YES ‡	NT	liver		72
88	Hemopexin	HPX	Matrisome Associated	ECM-affiliated	rbM	Abcam	ab124935	https://www.abcam.com/products/primary-antibodies/hemopexin-antibody-epr5610-ab124935.html?productWallTab=ShowAll	Tris, pH9.0	1:200-1:500	YES	NT	liver
89	Galectin 3 (Llectin, galactoside-binding, soluble, 3)	LGALS3	Matrisome Associated	ECM-affiliated	gt	R&D Systems	AF1154	https://www.rndsystems.com/products/human-galectin-3-antibody_af1154	Tris, pH9.0	1:1250	YES ‡	NT	colon, endometrium		96
90	Mucin 1	MUC1	Matrisome Associated	ECM-affiliated	rb	Abcam	ab15481	https://www.abcam.com/products/primary-antibodies/muc1-antibody-ab15481.html?productWallTab=ShowAll	Tris, pH9.0	1:400	YES ‡	YES ‡	lung		110, 111
91	Mucin 2	MUC2	Matrisome Associated	ECM-affiliated	rb	Santa Cruz Biotechnology	sc-15334	https://www.scbt.com/p/mucin-2-antibody-h-300?requestFrom=search	Citric, pH6.0	1:200	YES ‡	NO	colon		112
92	Mucin 16	MUC16	Matrisome Associated	ECM-affiliated	rbM	Abcam	ab110640	https://www.abcam.com/products/primary-antibodies/muc16-antibody-epr10202-ab110640.html?productWallTab=ShowAll	Tris, pH9.0	1:250-1:500	YES ‡	NT	endometrium		114
93	Plexin A2	PLXNA2	Matrisome Associated	ECM-affiliated	rb	Abcam	ab39357	https://www.abcam.com/products/primary-antibodies/plexin-a2-antibody-ab39357.html?productWallTab=ShowAll	Tris, pH9.0	1:200	YES ‡	YES ‡	colon, kidney, PDAC		118, 119
94	Syndecan 2	SDC2	Matrisome Associated	ECM-affiliated	rb	ABclonal	A1810	https://abclonal.com/catalog-antibodies/SDC2RabbitpAb/A1810	Tris, pH9.0	1:100	YES - but weak	NT	liver, lung
95	ADAM metallopeptidase 12	ADAM12	Matrisome Associated	ECM Regulators	rb	Proteintech	14139-1-AP	https://www.ptglab.com/products/ADAM12-Antibody-14139-1-AP.htm	Tris, pH9.0	1:200	YES ‡	YES	CRC, mouse TEB		2
96	ADAMTS metallopeptidase 17	ADAMTS17	Matrisome Associated	ECM Regulators	m	Novus Biologicals	H00170691-M01	https://www.novusbio.com/products/adamts17-antibody-3b7_h00170691-m01	Tris, pH9.0	1:3000	YES ‡	YES - but background stain	CRC, mouse TEB		3
97	Thrombin (coagulation factor II)	F2	Matrisome Associated	ECM Regulators	m	Abcam	ab17199	https://www.abcam.com/products/primary-antibodies/thrombin-antibody-5g9-ab17199.html?productWallTab=ShowAll	Tris, pH9.0	1:100	YES	NT	liver
98	Lysyl oxidase-like 2	LOXL2	Matrisome Associated	ECM Regulators	rb	Abcam	ab140833	https://www.abcam.com/products/primary-antibodies/loxl2-antibody-ab140833.html	Tris, pH9.0	1:200	YES ‡	YES ‡	cartilage, ovarian cancer	the indivudual antibodies show varying staining pattern	97, 98
99	Lysyl oxidase-like 2	LOXL2	Matrisome Associated	ECM Regulators	rb	Abcam	ab96233	https://www.abcam.com/products/primary-antibodies/loxl2-antibody-ab96233.html?productWallTab=ShowAll	Tris, pH9.0	1:250-1:500	YES	NT	cartilage, ovarian cancer	the indivudual antibodies show varying staining pattern
100	Lysyl oxidase-like 2	LOXL2	Matrisome Associated	ECM Regulators	rb	Novus Biologicals	NBP1-32954	https://www.novusbio.com/products/lysyl-oxidase-homolog-2-loxl2-antibody_nbp1-32954	Tris, pH9.0	1:250-1:500	YES	NT	cartilage, ovarian cancer	the indivudual antibodies show varying staining pattern