ABCDEFGHIJKLMNOPQRSTUVWX
1
#Each row is dedicated to a fully functional hamPCR primer set.
2
#Each primer set is annotated by keywords including, but not limited to, the primary organism(s), primer names (if meaningful), and the names of the genes you are targeting.
3
#Searching by ctrl + F and keywords can help you find appropriate primer sets.
4
#Please include any comments about functionality or special considerations, quirks, etc.
5
#Submit any new primer combinations and related information as comments in an empty row. Do not forget your ID as the contributor.
6
#We will check commented submissions for completeness and then paste them in the document.
7
8
Primer SetKeywordsCommentsReferenceContributor
Paired_Forward_HM-tagging_FASTA
Paired_Reverse_HM-tagging_FASTAForward_PCR_primer (barcodes as N)Reverse_PCR_primer (barcodes as N)
9
1Arabidopsis thaliana, bacteria, V4, 16S, 515F, 799R, Gigantea
Primers for 16S rDNA microbial load on A. thaliana hosts
Lundberg et al. hamPCR
Derek Lundberg
>515_F1_G-46603
TCCCTACACGACGCTCTTCCGATCTGAGTGYCAGCMGCCGCGGTAA
>At.GI_F1_G-46602
TCCCTACACGACGCTCTTCCGATCTGTAAAGATAAATGGGTCATCTAA
>799_R1_G-46601
GGAGTTCAGACGTGTGCTCTTCCGATCTTGCMGGGTATCTAATCCKGTT
>At.GI_R502bp_G-46614
GGAGTTCAGACGTGTGCTCTTCCGATCTTCCTTCTGAACCGGTGTATTC
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
10
2Arabidopsis thaliana, bacteria, V4, 16S, 515F, 806R, Gigantea
Primers for 16S rDNA microbial load on A. thaliana hosts
Lundberg et al. hamPCR
Derek Lundberg
>515_F1_G-46603
TCCCTACACGACGCTCTTCCGATCTGAGTGYCAGCMGCCGCGGTAA
>At.GI_F1_G-46602
TCCCTACACGACGCTCTTCCGATCTGTAAAGATAAATGGGTCATCTAA
>806_R1_G-46631
GGAGTTCAGACGTGTGCTCTTCCGATCTACGGACTACNVGGGTWTCTAAT
>At.GI_R502bp_G-46614
GGAGTTCAGACGTGTGCTCTTCCGATCTTCCTTCTGAACCGGTGTATTC
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
11
3Arabidopsis thaliana, bacteria, V3, V4, 16S, 341F, 799R, Gigantea
Primers for 16S rDNA microbial load on A. thaliana hosts
Lundberg et al. hamPCR
Derek Lundberg
>341_F1_G-46605
TCCCTACACGACGCTCTTCCGATCTGACCTACGGGAGGCAGCAG
>At.GI_F1_G-46602
TCCCTACACGACGCTCTTCCGATCTGTAAAGATAAATGGGTCATCTAA
>799_R1_G-46601
GGAGTTCAGACGTGTGCTCTTCCGATCTTGCMGGGTATCTAATCCKGTT
>At.GI_R502bp_G-46614
GGAGTTCAGACGTGTGCTCTTCCGATCTTCCTTCTGAACCGGTGTATTC
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
12
4Arabidopsis thaliana, bacteria, V5, V6, V7, 16S, 799F, 1192R, Gigantea
Primers for 16S rDNA microbial load on A. thaliana hosts
Lundberg et al. hamPCR
Derek Lundberg
>799_F1_G-46628
TCCCTACACGACGCTCTTCCGATCTGTAACMGGATTAGATACCCKG
>At.GI_F1_G-46602
TCCCTACACGACGCTCTTCCGATCTGTAAAGATAAATGGGTCATCTAA
>1192_R1_G-46629
GGAGTTCAGACGTGTGCTCTTCCGATCTGTACGTCATCCCCACCTTCC
>At.GI_R466bp_G-46652
GGAGTTCAGACGTGTGCTCTTCCGATCTAAGGGTTCAGCTTTGTCAACAA
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
13
5Arabidopsis thaliana, oomycete, ITS1-o, Gigantea (502bp piece)
Primers for oomycete load on A. thaliana hosts.
Lundberg et al. hamPCR
Derek Lundberg
>ITS1-O_F1_G-46636
TCCCTACACGACGCTCTTCCGATCTcggaaggatcattaccac
>At.GI_F1_G-46602
TCCCTACACGACGCTCTTCCGATCTGTAAAGATAAATGGGTCATCTAA
>5.8s-O_R1_G-46637
GGAGTTCAGACGTGTGCTCTTCCGATCTagcctagacatccactgctg
>At.GI_R502bp_G-46614
GGAGTTCAGACGTGTGCTCTTCCGATCTTCCTTCTGAACCGGTGTATTC
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
14
6Arabidopsis thaliana, oomycete, ITS1-o, Gigantea (466bp piece)
Primers for oomycete load on A. thaliana hosts
Lundberg et al. hamPCR
Derek Lundberg
>ITS1-O_F1_G-46636
TCCCTACACGACGCTCTTCCGATCTcggaaggatcattaccac
>At.GI_F1_G-46602
TCCCTACACGACGCTCTTCCGATCTGTAAAGATAAATGGGTCATCTAA
>5.8s-O_R1_G-46637
GGAGTTCAGACGTGTGCTCTTCCGATCTagcctagacatccactgctg
>At.GI_R466bp_G-46652
GGAGTTCAGACGTGTGCTCTTCCGATCTAAGGGTTCAGCTTTGTCAACAA
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
15
7Arabidopsis thaliana, oomycete, Hyaloperonospora, actin, Gigantea (502bp piece), V4, 16S
Primers for HPA load on A. thaliana host, with V4 16S rDNA at the same time.
Lundberg et al. hamPCR
Derek Lundberg
>515_F1_G-46603
TCCCTACACGACGCTCTTCCGATCTGAGTGYCAGCMGCCGCGGTAA
>Ha.Actin_F1_G-46716
TCCCTACACGACGCTCTTCCGATCT cgcgctgccgcacgcgattgt
>At.GI_F1_G-46602
TCCCTACACGACGCTCTTCCGATCTGTAAAGATAAATGGGTCATCTAA
>799_R1_G-46601
GGAGTTCAGACGTGTGCTCTTCCGATCTTGCMGGGTATCTAATCCKGTT
>Ha.Actin_R1_G-46717
GGAGTTCAGACGTGTGCTCTTCCGATCTcgccaaagccgtcagttccttc
>At.GI_R502bp_G-46614
GGAGTTCAGACGTGTGCTCTTCCGATCTTCCTTCTGAACCGGTGTATTC
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
16
8Triticum aestivum, fungus, ITS1F - ITS2, RNA polymerase I gene (PolA1)
Primers for fungal load on wheat plants
Lundberg et al. hamPCR
Derek Lundberg
>Ta.PolA1_F1_G-46750
TCCCTACACGACGCTCTTCCGATctgatgttgtggaaggaattgaa
>ITS1_F1_G-46622
TCCCTACACGACGCTCTTCCGATCTGGcttggtcatttagaggaagtaa
>Ta.PolA1_R1_G-46751
GGAGTTCAGACGTGTGCTCTTCCGAtctgcatcagctcccaagtc
>ITS2_R1_G-46623
GGAGTTCAGACGTGTGCTCTTCCGATCTCGgctgcgttcttcatcgatgc
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
17
9Pristionchus pacificus, Calsequestrin gene (csq-1), bacteria, V5, V6, V7, 16S, 799F, 1192R
Primers for bacterial load in Pristionchus pacificus
Lundberg et al. hamPCR
Derek Lundberg
>Pp.csq-1_F1_G-46691
TCCCTACACGACGCTCTTCCGATCTcatcgagaatatatggccgat
>799_F1_G-46628
TCCCTACACGACGCTCTTCCGATCTGTAACMGGATTAGATACCCKG
>Pp.csq-1_R1_G-46692
GGAGTTCAGACGTGTGCTCTTCCGATCTtgagttatgccccactattcaa
>1192_R1_G-46629
GGAGTTCAGACGTGTGCTCTTCCGATCTGTACGTCATCCCCACCTTCC
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
18
10Capsicum annuum, bacteria, V4, 16S, 515F, 799R, Gigantea
Primers for 16S rDNA microbial load on C. annuum hosts
Lundberg et al. hamPCR
Derek Lundberg
>515_F1_G-46603
TCCCTACACGACGCTCTTCCGATCTGAGTGYCAGCMGCCGCGGTAA
>Ca.GI_F1_G-46626
TCCCTACACGACGCTCTTCCGATCTcaaatgattcatctattgagcta
>799_R1_G-46601
GGAGTTCAGACGTGTGCTCTTCCGATCTTGCMGGGTATCTAATCCKGTT
>Ca.GI_R1_G-46627
GGAGTTCAGACGTGTGCTCTTCCGATCTctcctttagaactggtgcatg
>PCR_F_G-40610
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
>PCR_hamPCR_R
CAAGCAGAAGACGGCATACGAGATNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTC
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100