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Addgene submissionNameDescriptionConcentrationDateBOXSequence
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3BicDR1-SNAPBicDR1-SNAP cloned by Mohi80 ng/ul4/23/2019LUKEAsk MOHI
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4Kinesin/HTUsed in prep LF2 (sequenced)about 50 ng/uL8/17/2015LUKEModified from KGH (seq below)
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7KGHFRANK'S (aliquot) pfc20 *Amp124 ng/uLoldLUKEhttps://drive.google.com/open?id=1Q3sOMAFSMCXGqHcov79GBgY0pkArGif3
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8889GFP-dynein (GFP on tail)254 ng/ul11/9/2016LUKESee mammalian dynein seq information folder
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9908SNAP-dynein (N-terminal) 303 ng/ul11/9/2016LUKESee mammalian dynein seq information folder
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101539BicD2-GFP (C term)100 ng/ul5/1/2018LUKEhttps://drive.google.com/open?id=1q8-HcoaVDuzHnLxJzmKZQiXaeFFyXUon
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111792BicD2-SNAP (C term)173 ng/ul11/9/2016LUKEhttps://drive.google.com/open?id=1F8x1JTLkO6RQO4OdE4TDolnaOr_W3-AX
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121794BicDR1-GFP (C term)265 ng/ul11/9/2016LUKENA
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13K560GFP from Vale lab (bacterial expression)K560 GFP
pET17_K560_GFP_6His, contains cysteins.
Cloning Vector: pET17b
ampicillin 		N/A6/21/2017LUKENA
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15Full length DCXflDCX from Houdusse lab in Paris1 ug/ul02/17LUKENA
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16Truncated DCXtDCX from Houdusse lab in Paris1 ug/ul02/17LUKENA
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17Full length Drosophila kinesinBACTERIAL EXPRESSION - Plasmid DNA - dmKHC - His tagged - Diez lab64 ng/ul06/17LUKENA
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18Full length, constitutively active human kinesin-1 from Vale lab (insect cell expression)
PfastK963, delta hinge
See Dara's paper for details. Should be located elsewhere too.
Source: Dara Friedman
Insert Name: full length kinesin with the hinge region removed as per her paper. Cloning Vector: PfastBac
ampicillin
400 ng/uL08/11/17LUKENA
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19Miniprep of phi dynein plasmid from Carter lab - p1747Human dynein - phi interface mutant225 ng/ul08/11/17LUKESee mammalian dynein seq information folder
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242N4R Tau Nogales (original from Simon)pET3D vector with 2N4R human tau 441 insert - may have restriction sites - The human tau isoform used is 441 residues long and is referred to as isoform-F
(UNIPROT P10636-8)		not knownaliquot 5/17/18LUKENA
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27TPP1 TIN2 coexpressionpetduet vector for coexpression of TPP1 Tin2 - E. coli expression - should have FLAG tag for complex purification173 ng/ulLUKEDO NOT USE
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28Tau 441 (no GFP) pOmniBacTau in the pOmniBac vector - sequence verified cloning junctions in both directions - clone one75 ng/ulLUKEhttps://drive.google.com/open?id=1xU3ME31slM46pOomk6Yy76S-1wsegTSy
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32TRF2 cDNAMGC Human TERF2 Sequence-Verified cDNA (CloneId:4346819), glycerol stock420 ng/uLDharmaconLUKESee dharmacon website
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33Rap1 cDNAMGC Human TERF2IP Sequence-Verified cDNA (CloneId:2820760), glycerol stock271 ng/ulDharmaconLUKESee dharmacon website
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34Tin2 cDNAMGC Human TINF2 Sequence-Verified cDNA (CloneId:3505009), glycerol stock211 ng/uLDharmaconLUKESee dharmacon website
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35TPP1 cDNAMGC Human ACD Sequence-Verified cDNA (CloneId:4400396), glycerol stock115 ng/uLDharmaconLUKESee dharmacon website
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36Pot1 cDNAMGC Human POT1 Sequence-Verified cDNA (CloneId:3955573), glycerol stock244 ng/ulDharmaconLUKESee dharmacon website
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38TEV protease plasmidPlasmid from the macro lab - originally addgene Plasmid #8827 - Encodes optimal tev protease with MBP tag : TEV linker : HIS tag : TEV protein - AMP resistant - got from Mohi70 ng/ulLUKEhttps://www.addgene.org/8827/
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40Dharmacon MAP7MGC Human MAP7
Sequence-Verified cDNA
(CloneId:5222987)
376 ng/uLLUKESee dharmacon website
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41MBP fastbacLIC MBP pFastBac (addgene: 30116)285 ng/uLLUKESee addgene
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42MAP7 - pOmniBacFull length MAP7 (insert from Dharmacon), no GFP, pOmnibac - clone 1 - F sequence verified, and PCR looked good130 ng/ulLUKEhttps://drive.google.com/open?id=1a4LWu11SnX--i7N-C51eZQaqZ6klIrQT
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43pLIB - MBP TIN2clone 5, sequence F verified, PCR correct size100 ng/ulBIGBACLUKEhttps://drive.google.com/open?id=1JSldw5YD83qJ26pfFulzTsgyM5_oRp73
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44pLIB - MBP TPP1clone 10, sequence F verified, PCR correct size80 ng/ulBIGBACLUKEhttps://drive.google.com/open?id=1MtEj1D7j3-Z791NlrI-mUjyRMnl8XxSA
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45pLIB - ZZ POT1N-terminal ZZ tag on pot1140 ng/ulBIGBACLUKEhttps://drive.google.com/open?id=13L6wkEZStIcoMwYK1JyI0woBETFdFcWN
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46pLIB - TPP1no MBPlowBIGBACLUKEDO NOT USE (for expression - MBP is required)insert fine tho
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47pLIB - TIN2no MBPlowBIGBACLUKEDO NOT USE (for expression - MBP is required)insert fine tho
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48pLIB - TRF2lowBIGBACLUKEDO NOT USE (for expression - ZZ is required)insert fine tho
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49pLIB - RAP1lowBIGBACLUKEhttps://drive.google.com/open?id=1w24y89DZ1S_h-M9si96qQQMQUv5I6ZKm
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51pBIG1aAMP and SPEC !! - https://www.addgene.org/80611/ - not digested 92ng/ulLUKE2See addgene
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52Full assembly BigBac MBP clone 4TRF2, Rap1, His:MBP:TEV:Tin2, His:MBP:TEV:Tpp1, ZZ-POT1 - used this one in the prepBIGBACLUKE2NA
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53Full assembly BigBac MBP clone 5TRF2, Rap1, His:MBP:TEV:Tin2, His:MBP:TEV:Tpp1, ZZ-POT1 - backup clone - digested fine, but there was a faint extra band that may be a problem.BIGBACLUKE2NA
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54SFP for ybbR tag labellingAddgene 75015, Sfp pet29b C-terminal His Tag50 ng/ulAddgeneLUKE2See addgene
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55pLIB - ZZ TRF2clone 15, right R sequence, right sizeLUKE2https://drive.google.com/open?id=1XW1Z0DcB6tFlywR_6ZVJaEKkqPMpgH9sinsert seq only
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YESpOmnibac_ZZ_linker_ybbR_Tau56pOmnibac-ybbR-TauZZ - TEV - ybbr - Tau 44135 ng/ulLUKE2https://drive.google.com/open?id=16Dsorfup8ZW5wt6WShG_UnnylTpffTochttps://benchling.com/s/seq-dh27dLt3oQqI6hyQ9orN?m=slm-T9dj23XT0amA8V0ztyB8
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YESpOmnibac_ZZ_linker_ybbR_MAP757pOmnibac-ybbR-MAP7ZZ - TEV - ybbr - MAP7 FL45 ng/ulLUKE2https://drive.google.com/open?id=16vWg8jyhBhdh0aE6G2sMXnzLqxGDV7tM
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58MAP7 p123 (Brooks)MAP7 (aa 175-316) pOmniBac, N-terminal ybbRMacro lab madeLUKE2https://drive.google.com/open?id=1JCplUsyxyuLS86JteJ78xrV4gziz6Q3W
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59MAP7 MTBD (Brooks)MAP7 (aa 60-170) pOmniBac, N-terminal ybbRMacro lab madeLUKE2https://drive.google.com/open?id=1mBszAu3TjE5Pi08pZPDToRVuXaZKHaBK
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60MAP7 C-terminal (Brooks)MAP7 (aa 307 - 749) pOmniBac, N-terminal ybbRMacro lab madeLUKE2https://drive.google.com/open?id=1Q-u_5sMTuAuvEge_UZDdnemSt6c5-zoV
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YESpOmnibac_ZZ_linker_ybbR_Tau MTBD61Tau MTBD (Brooks)Tau (aa 242 - 367) pOmniBac, N-terminal ybbRMacro lab madeLUKE2https://drive.google.com/file/d/14HHkZwbO3cdpHduAPi09bBqIl1psTqhN/view?usp=sharing
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YESpOmnibac_ZZ_linker_K560_GFP_SNAP62K560 pOmnibac (Brooks)KGH (cysteine's included) K560-GFP-SNAPMacro lab madeLUKE2https://drive.google.com/file/d/1B5rJH2_gJ5ORFMEP6eNbsE9QL1FU7Iwg/view?usp=sharing
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YESpOmnibac_ZZ_linker_K490_GFP_SNAP 63K490 pOmnibacKGH (cysteine's included) K490-GFP-SNAP150 ng/ulLUKE2https://drive.google.com/file/d/1PAPVvNxYJD2N3-BdBYUF1pS6Pb9H1xiX/view?usp=sharing
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64N-ybbR 1xR2 only Tau pOmniBacNOTE - NO PGGG LINKER N-ybbR 1xR2 only Tau pOmniBac 150 ng/ulLUKE2https://drive.google.com/open?id=1JcOC_qjw9C4YwW9DXH3b16fXJZpSSJPh
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65N-ybbR PGGG LINKER 1xR2 only Tau pOmniBacN-ybbR PGGG LINKER 1xR2 only Tau pOmniBac150 ng/ulLUKE2https://drive.google.com/open?id=1mXMf1dE2KYvPvDXFzWdUhqOT3QesCs6n
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66Miniprep of Dharmacon TTLL7Nicole164 ng/ulLUKE2See Dharmacon website
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YESpOmnibac_ZZ_linker_MAP7_N67N-ybbR MAP7 N-terminal onlyN-ybbR MAP7 N-terminal only - pOmnibac - includes MTBD and P123150 ng/ulLUKE2https://drive.google.com/open?id=1XhQBUPBYQuzQ9Fr2LUCnoPWziwH-oJCd
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69Traptavidin pET21a-Traptavidin (addgene 24753)100 ng/ulLUKE2See addgene, amp reshttps://benchling.com/s/seq-xcaq1zMeliI5JwJbUHkE
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70Stu2 1-590pGEX-6P-1 Stu2 1-590 (addgene 38314)80 ng/ulLUKE2see addgene
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71ANP32A from DharmaconMGC Human ANP32A Sequence-Verified cDNA, miniprep150 ng/uLLUKE2See Dharmacon website
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72TTLL7 1-518 (final)TTLL7 1-518 (aa 161 now P, as in Mecak sequence) - sequenced F and R, including AA 16180 ng/ulLUKE2https://drive.google.com/open?id=1e1nnqzRHBKHTG-eCkQna4KF6q6Axj6vn
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73Monomeric KinesinpBJL27 Kif5B 1-360 E236A (amp, IPTG) (Actually 1-349)100 ng/ulAMPLUKE2https://benchling.com/s/seq-VSnfxH67gj1BENRTptfj?m=slm-Pjn7SZdortftzlGq2CBQhttps://drive.google.com/drive/folders/1kZ71BbqIoi69RW8b0dAfuTwGXuUnypcK?usp=sharingNOTE: this plasmid has a few AA changes other than E236A relative to the KIF5B sequence we use. But it's documented on Addgene #60909 from the Vale lab, so I assume it's fine
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74C-terminal : BicDR1 SNAP K491-560 BicKin pOmnibac - N and C terminal sequence junctions130 ng ulLUKE2
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75N-terminal : BicDR1 SNAP K491-560 Kin490-560 then BICDR1 pOmnibac - N and C terminal sequence junctions - clone 2LUKE2
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76hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIhttps://www.addgene.org/83928/110 ng/ulAMPLUKE2https://www.addgene.org/83928/
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77SRS-dynein stalk MTBD fusion (85/82) MonomerMiniprep of pFC7, can seq with T7 --- should map to genbank FJ039886 --- from Structure and Functional Role of Dynein’s Microtubule-Binding Domain --- actually seems to be codon optimized and cys light - but the amino acid sequence is correct80 ng/ulKANLUKE2
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79Addgene GFP nanobody SNAPhttps://www.addgene.org/82711/ pBiex-1 GFPNanobody_SNAP110 ng/ulLUKE2https://www.addgene.org/82711/
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80SNAP-Lis1From Simon Bullock - insect cell expression - Mohi says this plasmid works200LUKE2NA
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81K490 stalkpBiex_K490 stalk_GFP_His for E. coli expression - clone 42180 ng/ulAMPLUKE2https://drive.google.com/open?id=1qffwbDFZRIy-RkQigQ-PQi_8vwmdgqho
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82K560 stalkpBiex_K560 stalk_GFP_His for E. coli expression - clone 52180 ng/ulAMPLUKE2https://drive.google.com/open?id=1lJl7IyZy2u3nP4oj6oDoDX1WcYwYltOU
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83DCX-GFP (addgene)human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II144 ng/uLAMPLUKE2http://www.addgene.org/83918/
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84Lis1 (addgene)mammalian expression of PAFAH1B1 (Lis1) - to be used for subcloningAMPLUKE2https://www.addgene.org/92430/
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85ybbR -ANP32ApOmnibac N-ybbr ANP32A -- fully sequenced - clone 1200 ng/uLGENLUKE2https://drive.google.com/open?id=1rziqoQwu8Tozj7DiDK55U2MnbTi4Pym5
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86Jip1 cDNA from DharmaconJip1 cDNA from Dharmacon166 ng/ulAMP LUKE2
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87pHAT-ZZ-TEV-ybbRE. coli protein expression vector - ZZ and ybbr - pHAT backbone - fully sequenced clone 250 ng/ulAMPLUKE2https://drive.google.com/open?id=1L4yNnVgKOyptgJmo8ZxH3_8VA0RjacY3
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88pHAT ZZ Random PosInsert 30K 30R 20G 20S - fully sequenced - pHAT backbone with ZZ Tev Ybbr on N terminus - 100 aa insert - clone 3250 ng/uLAMPLUKE2https://drive.google.com/open?id=1DMak68GADdPhuPx2a7YpDwvIZjCl5BEr
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YESpOmnibac_ZZ_linker_K963_GFP_SNAP 89FL KIF5B-GFP-SNAPJohn plasmid 78 - Full length KIF5B GFP SNAP - no point mutation - pOMNIBAC1 ng/uLGENLUKE2
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90R2 tau scramblepHAT ZZ ybbr R2 scramble - seq confirmed250 ng/ulAMPLUKE2
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91M7 mtbd scramblepHAT ZZ ybbr MAP7 MTBD scramble - seq confirmed250 ng/ulAMPLUKE2
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92M7 Nterm scramblepHAT ZZ ybbr MAP7 N-terminal scramble - seq confirmed250 ng/ulAMPLUKE2
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93INSERT - tau MTBD scramble Genscript - corresponds to "Genscript fragment (LF93)" in the benchling file - insertion of scrambled sequence in the full length protein40 ng/uLAMPLUKE2https://benchling.com/s/seq-jAmaJmP2td6UuQ5VhYI7
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94INSERT - MAP7 Nterm scrambleGenscript - corresponds to "Genscript fragment (LF94)" in the benchling file - insertion of scrambled sequence in the full length protein40 ng/uLAMPLUKE2https://benchling.com/s/seq-U3UyznXoWNL924MRVbZA
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95bicdr-snap-nano without gs linkerDO NOT USE, cloning intermediateLUKE2
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96mohi phi29 polymerase
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