EM gridbox/sample manager

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P
1	OT1			Next box:		Free boxes:		Stored and Screened	trashed	Stored and not Screened	To Be Screened	Stored for Krios	Data collection Krios	Data collection Arctica
2	Gridbox	Gridbox position	Gridbox storage	Date frozen	Date screened	Sample	Blotting conditions	plasma cleaning	Grid type	Notes	Data collected?	Notes from CEMRC	# of images collected	Processed?	Particles	Resolution?

3	S5	1	Santi #2	25/10/17	27/10/17 (Jeol 1400)	0.6uM F-Actin 0.05% NP-40	4s (Leica)	6s 25W H2/02	400 C-flat	Thick Ice, no filaments	no
4	S5	2	Santi #2	25/10/17	27/10/17 (Jeol 1400)	0.6uM F-Actin 0.05% NP-40	3s (Leica)	6s 25W H2/02	400 C-flat	lost in transfer	no
5	S5	3	Santi #2	25/10/17	27/10/17 (Jeol 1400)	0.6uM F-Actin 0.05% NP-40	4s (Leica)	6s 25W H2/02	300 C-flat	Thick Ice, some filaments	no
6	S5	4	Santi #2	25/10/17	27/10/17 (Jeol 1400)	0.6uM F-Actin 0.05% NP-40	3s (Leica)	6s 25W H2/02	300 C-flat	lost in transfer	no
7	S6	1	Santi #2	25/10/17	27/11/17 (Arctica)	0.6uM F-Actin 0.05% NP-40	3s (Leica)	6s 25W H2/02	200 C-flat	Dirty/contaminated, old 200C grids are bad	no
8	S6	2	Santi #2	25/10/17	27/11/17 (Arctica)	0.6uM F-Actin 0.05% NP-40	4s (Leica)	6s 25W H2/02	200 C-flat	Dirty/contaminated, old 200C grids are bad	no
9	S6	3	Santi #2	25/10/17	27/11/17 (Arctica)	0.6uM F-Actin 0.05% NP-40	3s (Leica)	6s 25W H2/02	200 Au C-flat	Good ice thickness, actin density low but overall good filaments	no
10	S6	4	Santi #2	25/10/17	27/11/17 (Arctica)	0.6uM F-Actin 0.05% NP-40	4s (Leica)	6s 25W H2/02	200 Au C-flat	Too many broken squares, actin filament density is too low	no
11	S7	1	GMA05 Pos5	25/10/17		0.6uM F-Actin 0.02% NP-40	3s (Leica)	6s 25W H2/02	300 C-flat
12	S7	2	GMA05 Pos5	25/10/17		0.6uM F-Actin 0.02% NP-40	4s (Leica)	6s 25W H2/02	300 C-flat
13	S7	3	Santi #2	25/10/17	31/10/17 (Jeol 1400)	0.6uM F-Actin 0.01% NP-40	3s (Leica)	6s 25W H2/02	200 Au	Little contamination, good filaments, good ice thickness	NO
14	S7	4	Santi #2	25/10/17	31/10/17 (Jeol 1400)	0.6uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	200 Au	Little contamination, good filaments, good ice thickness	NO
15	P29	1	Santi #2	25/10/17		0.6uM F-Actin 0.02% NP-40	3s (Leica)	6s 25W H2/02	400 C-flat
16	P29	2	Santi #2	25/10/17		0.6uM F-Actin 0.02% NP-40	4s (Leica)	6s 25W H2/02	400 C-flat
17	P29	3	Santi #2	25/10/17		0.6uM F-Actin 0.02% NP-40	3s (Leica)	6s 25W H2/02	200 C-flat
18	P29	4	Santi #2	25/10/17		0.6uM F-Actin 0.02% NP-40	4s (Leica)	6s 25W H2/02	200 C-flat
19	S5	1	Santi #2	1/11/17		0.6uM F-Actin 0.05% NP-40	3s (Leica)	6s 25W H2/02	200 Au C-flat
20	S5	2	Santi #2	1/11/17		0.6uM F-Actin 0.05% NP-40	4s (Leica)	6s 25W H2/02	200 Au C-flat
21	S5	3	Santi #2	1/11/17	27/11/17	0.6uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	300 UltrAuFoil	Not enough contrast, but still able to see filaments
22	S5	4	Santi #2	1/11/17	27/11/17	0.6uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	300 UltrAuFoil	Not enough contrast, but still able to see filaments
23	P28	1	Santi #2	1/11/17	27/11/17 (Arctica)	0.6uM F-Actin 0.01% NP-40	3s (Leica)	6s 25W H2/02	200 Au C-flat	Actin density is a bit low, but good contrast and nice ice thickness (Best from screen session on 27/11/17)	NO
24	P28	2	Santi #2	1/11/17		0.6uM F-Actin 0.01% NP-40	3s (Leica)	6s 25W H2/02	200 Au C-flat
25	P28	3	Santi #2	1/11/17	27/11/17 (Arctica)	0.6uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	200 Au C-flat	Actin density is a bit low, but good contrast and nice ice thickness	NO
26	P28	4	Santi #2	1/11/17		0.6uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	200 Au C-flat
27	L29	1	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	3s (Vitrobot)	15s 50W air	200 Au C-flat	Concentration of LATS too high	NO
28	L29	2	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	4s (Vitrobot)	15s 50W air	200 Au C-flat	Concentration of LATS too high	NO
29	L29	3	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	5s (Vitrobot)	15s 50W air	200 Au C-flat	Concentration of LATS too high	NO
30	L29	4	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	6s (Vitrobot)	15s 50W air	200 Au C-flat	Concentration of LATS too high	NO
31	L17	1	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	3s (Vitrobot)	15s 50W air	300 C-flat	Concentration of LATS too high	NO
32	L17	2	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	4s (Vitrobot)	15s 50W air	300 C-flat	Concentration of LATS too high	NO
33	L17	3	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	5s (Vitrobot)	15s 50W air	300 C-flat	Concentration of LATS too high	NO
34	L17	4	Santi #2	15/11/17	27/11/17 (Arctica)	0.2mg/mL LATS	6s (Vitrobot)	15s 50W air	300 C-flat	Concentration of LATS too high	NO
35	L29	1	GMA05 Pos3	30/11/17		0.1mg/mL LATS	3s (Vitrobot)	15s 50W air	300 Au C-flat
36	L29	2	GMA05 Pos3	30/11/17		0.1mg/mL LATS	3s (Vitrobot)	15s 50W air	300 Au C-flat
37	L29	3	GMA05 Pos3	30/11/17		0.1mg/mL LATS	5s (Vitrobot)	15s 50W air	300 Au C-flat
38	L29	4	GMA05 Pos3	30/11/17		0.1mg/mL LATS	5s (Vitrobot)	15s 50W air	300 Au C-flat
39	23	1	GMA05 Pos10	30/11/17		0.05mg/mL LATS	3s (Vitrobot)	15s 50W air	300 Au C-flat
40	23	2	GMA05 Pos10	30/11/17		0.05mg/mL LATS	3s (Vitrobot)	15s 50W air	300 Au C-flat
41	23	3	GMA05 Pos10	30/11/17		0.05mg/mL LATS	5s (Vitrobot)	15s 50W air	300 Au C-flat
42	23	4	GMA05 Pos10	30/11/17		0.05mg/mL LATS	5s (Vitrobot)	15s 50W air	300 Au C-flat
43	S10	1	Santi #2	30/11/17		0.05mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat
44	S10	2	Santi #2	30/11/17		0.05mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat
45	S10	3	Santi #2	30/11/17		0.0125mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat
46	S10	4	Santi #2	30/11/17		0.0125mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat
47	S11	1	Santi #2	30/11/17	11/12/17 (Krios)	1uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	300 Au C-flat	Too many broken squares. Possibility that sample was applied on wrong side of grid	NO
48	S11	2	Santi #2	30/11/17	11/12/17 (Krios)	1uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	300 Au C-flat	Too many broken squares. Possibility that sample was applied on wrong side of grid	NO
49	S11	3	Santi #2	30/11/17	11/12/17 (Krios)	1uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	300 Au C-flat	Too many broken squares. Possibility that sample was applied on wrong side of grid	NO
50	S12	1	GMA05 Pos12	7/12/17	11/12/17 (Krios)	1uM F-Actin 0.01% NP-40	3s (Leica)	6s 25W H2/02	300 Au C-flat	Best grid that I have plunged. Great F-actin concentration, Ice is not too thick, and contrast is good. Used one week old actin	YES	Stored in Yellow-1 (Rui 11/12/18)
51	S12	2	GMA05 Pos12	7/12/17	11/12/17	1uM F-Actin 0.01% NP-40	3s (Leica)	6s 25W H2/02	300 Au C-flat			Stored in Yellow-2 (Rui 11/12/18)
52	S12	3	GMA05 Pos12	7/12/17	11/12/17	1uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	300 Au C-flat			Stored in Yellow-3 (Rui 11/12/18)
53	S12	4	GMA05 Pos12	7/12/17	11/12/17	1uM F-Actin 0.01% NP-40	4s (Leica)	6s 25W H2/02	300 Au C-flat			Stored in Yellow-4 (Rui 11/12/18)
54	S13	1	Santi #2	13/12/17	15/1/18 (Arctica)	0.1mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat	Most squares broken few viable squares but seem to contain LATS at good density		Stored in Orange-1 (15/01/18)
55	S13	2	Santi #2	13/12/17	15/1/18 (Arctica)	0.05mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat	Most squares broken few viable squares but seem to contain LATS at good density		Stored in Orange-2 (15/01/18)
56	S13	3	Santi #2	13/12/17	15/1/18 (Arctica)	0.1mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	Most squares broken few viable squares but seem to contain LATS at good density
57	S13	4	Santi #2	13/12/17	15/1/18 (Arctica)	0.05mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	Most squares broken few viable squares but seem to contain LATS at good density
58	S11	1	Santi #2	13/12/17	26/03/18 (Arctica)	0.1mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	not screened yet		Stored in White-1 (Ayala 22/03/18)
59	S11	2	Santi #2	13/12/17	26/03/18 (Arctica)	0.05mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	not screened yet		Stored in White-2 (Ayala 22/03/18)
60	S11	3	Santi #2	13/12/17	26/03/18 (Arctica)	0.025mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat	not screened yet		Stored in White-3 (Ayala 22/03/18)
61	S11	4	Santi #2	13/12/17	26/03/18 (Arctica)	0.025mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	not screened yet		Stored in White-4 (Ayala 22/03/18)
62	S12	1	Santi #2	15/12/17	15/1/18 (Arctica)	1 uM F-Actin + Myo VI 13 uM 0.01% NP-40	3s (Leica)	6s 25W H2/02	300 Au C-flat	Myosin concentration was too high; filaments were still present
63	S12	2	Santi #2	15/12/17		1 uM F-Actin + Myo VI 13 uM 0.01% NP-40	3s (Leica)	6s 25W H2/02	300 Au C-flat	Too bent to be screened
64	S12	3	Santi #2	15/12/17	15/1/18 (Arctica)	1 uM F-Actin + Myo VI 13 uM 0.01% NP-40	5s (Leica)	6s 25W H2/02	300 Au C-flat	Myosin concentration was too high; filaments were still present
65	S12	4	Santi #2	15/12/17	15/1/18 (Arctica)	1 uM F-Actin + Myo VI 13 uM 0.01% NP-40	5s (Leica)	6s 25W H2/02	300 Au C-flat	Myosin concentration was too high; filaments were still present
66	S14	1	Santi #2	11/1/18	15/1/18 (Arctica)	0.2 mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat	Too high density of LATS but grids fairly well conserved		Stored in Orange-3 (15/01/18)
67	S14	2	Santi #2	11/1/18	15/1/18 (Arctica)	0.2 mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	Too high density of LATS but grids fairly well conserved		Stored in Orange-4 (15/01/18)
68	S14	3	Santi #2	11/1/18	15/1/18 (Arctica)	0.1 mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat	Most squares are broken no sign of intact LATS protein in holes
69	S14	4	Santi #2	11/1/18	15/1/18 (Arctica)	0.1 mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	Most squares are broken no sign of intact LATS protein in holes
70	S15	1	Santi #2	11/1/18	15/1/18 (Arctica)	0.05mg/mL LATS	4s (Leica)	6s 25W H2/02	300 Au C-flat	Not good grid quality, most squares broken or LATS was destroyed during freezing process
71	S15	2	Santi #2	11/1/18	15/1/18 (Arctica)	0.05mg/mL LATS	5s (Leica)	6s 25W H2/02	300 Au C-flat	Not good grid quality, most squares broken or LATS was destroyed during freezing process
72	S15	3	Santi #2	11/1/18	15/1/18 (Arctica)	0.05mg/mL LATS	3s (Leica)	6s 25W H2/02	300 Au C-flat	Not good grid quality, most squares broken or LATS was destroyed during freezing process
73	S15	4	Santi #2	11/1/18	15/1/18 (Arctica)	0.1 mg/mL LATS	5s (Leica)	6s 25W H2/02	300 Au C-flat	Not good grid quality, most squares broken or LATS was destroyed during freezing process
74	S16	1	Santi #2	11/1/18		0.3uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat
75	S16	2	Santi #2	11/1/18		0.3uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat
76	S16	3	Santi #2	11/1/18	07/02/18 (Arctica)	0.6uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat
77	S16	4	GMA03 Pos8	11/1/18	07/02/18 (Arctica)	0.6uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat			Stored in Black-1 (Santi Arctica 02-07-18)
78	S17	1	GMA05 Pos8	11/1/18		0.3uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat
79	S17	2	GMA05 Pos8	11/1/18		0.3uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat
80	S17	3	GMA05 Pos8	11/1/18		0.6uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat
81	S17	4	GMA05 Pos8	11/1/18		0.6uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat
82	S18	1	GMA02 Pos7	12/1/18	05/03/18 (Krios)	1.0uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat			Stored in Red-1 (Santi 02-21-18)
83	S18	2	GMA02 Pos7	12/1/18	05/03/18 (Krios)	1.0uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat			Stored in Red-2 (Santi 02-21-18)
84	S18	3	GMA03 Pos8	12/1/18	07/02/18 (Arctica)	1.3uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat	Ice seems too thick even in squares which appear to have thin ice; some contamination		Stored in Black-2 (Santi Arctica 02-07-18)
85	S18	4	GMA03 Pos8	12/1/18	07/02/18 (Arctica)	1.3uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat	Ice seems too thick even in squares which appear to have thin ice; some contamination		Stored in Black-3 (Santi Arctica 02-07-18)
86	S19	1	GMA02 Pos10	12/1/18	05/03/18 (Krios)	1.0uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat			Stored in Yellow-1 (Santi 02-21-18)
87	S19	2	GMA02 Pos10	12/1/18	05/03/18 (Krios)	1.0uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat	Krios data collected with this grid, other grids in this Krios session are all saved. -Lin		Stored in Yellow-2 (Santi 02-21-18)
88	S19	3	GMA02 Pos10	12/1/18	05/03/18 (Krios)	1.3uM F-Actin + 10uM Mvt	3s (Leica)	6s 25W H2/02	300 Au C-flat	Not screened; but put in Krios (NEED TO BE CLIPPED)		Stored in Yellow-3 (Santi 02-21-18)
89	S19	4	GMA02 Pos10	12/1/18	05/03/18 (Krios)	1.3uM F-Actin + 10uM Mvt	5s (Leica)	6s 25W H2/02	300 Au C-flat	Not screened; but put in Krios (NEED TO BE CLIPPED)		Stored in Yellow-4 (Santi 02-21-18)
90	S6	1	Santi #2	18/1/18		0.3uM F-Actin + 10uM MvtP	3s (Leica)	6s 25W H2/02	300 Au C-flat
91	S6	2	Santi #2	18/1/18		0.3uM F-Actin + 10uM MvtP	3s (Leica)	6s 25W H2/02	300 Au C-flat
92	S6	3	Santi #2	18/1/18	07/02/18 (Arctica)	0.6uM F-Actin + 10uM MvtP	3s (Leica)	6s 25W H2/02	300 Au C-flat	Many squares intact, ice thickness looks okay; however, no filaments can be observed
93	S6	4	Santi #2	18/1/18		0.6uM F-Actin + 10uM MvtP	3s (Leica)	6s 25W H2/02	300 Au C-flat
94	S12	1	Santi #2	18/1/18		0.3uM F-Actin + 10uM MvtP	5s (Leica)	6s 25W H2/02	300 Au C-flat
95	S12	2	Santi #2	18/1/18		0.3uM F-Actin + 10uM MvtP	5s (Leica)	6s 25W H2/02	300 Au C-flat
96	S12	3	Santi #2	18/1/18	07/02/18 (Arctica)	0.6uM F-Actin + 10uM MvtP	5s (Leica)	6s 25W H2/02	300 Au C-flat	Many squares intact, ice thickness looks okay; however, no filaments can be observed
97	S12	4	Santi #2	18/1/18		0.6uM F-Actin + 10uM MvtP	5s (Leica)	6s 25W H2/02	300 Au C-flat
98	S4	1	GMA02 Pos6	26/01/18		1.0uM F-actin + 10uM MvtP	5s (Leica)	9s 25W H2/02	300 Au C-flat
99	S4	2	GMA02 Pos6	26/01/18		1.0uM F-actin + 10uM MvtP	5s (Leica)	9s 25W H2/02	300 Au C-flat
100	S4	3	GMA02 Pos6	26/01/18		1.3uM F-actin + 10uM MvtP	5s (Leica)	9s 25W H2/02	300 Au C-flat