BioE140L-Spring_2010_SequencingLog
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platewellOligoConstructdataReceivedAnalysisAdditional Read RequiredUserJCA_Note
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sbb10 Basic Parts AA1ca998pBjk2741-sbb123/10/2010perfectASaarniConfirmed
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sbb10 Basic Parts AB1ca998pBjk2741-sbb123/10/2010perfect (possible mutation in EcoR1 site)ASaarnidropped
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sbb10 Basic Parts AC1ca998pBjk2741-sbb163/10/2010perfectnoKai Mesa
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sbb10 Basic Parts AD1ca998pBca9523-sbb273/10/2010possible 1 bp insertion at BglII site, silent mutation in cds(349bp)yes
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sbb10 Basic Parts AE1ca998pBjk2741-sbb423/10/2010perfect (possible mutation in EcoR1 site)Jenna KelleherConfirmed
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sbb10 Basic Parts AF1ca998pBca9523-sbb243/10/2010perfect partial (1 silent mutation, 1 point mutation)yesCarolyn KwokSequencing 2nd clone
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sbb10 Basic Parts AG1ca998pBjk2741-sbb323/10/2010perfectCarolyn KwokConfirmed
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sbb10 Basic Parts AH1ca998pBca9523-sbb303/10/20102 point mutations (bp:79, 390)yesJose GutierrezSequencing 2nd clone
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sbb10 Basic Parts AA2ca998pBjk2741-ig1143/10/2010perfect partial (2 point mutations)Eco/Bam transfer doesn't nee reread Jose GutierrezConfirmed
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sbb10 Basic Parts BF1ca998pBca9523-sbb243/12/20102 point mutationsnoCarolyn Kwokmodify model, keep this clone
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sbb10 Basic Parts BH1ca998pBca9523-sbb303/12/2010possible deletion at bp 47, also looks like contaminated DNA, also c-t mutation at 126yesJose Gutierrez
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sbb10 Basic Parts BD1ca998pBca9523-sbb273/12/2010silent mutation (349bp A->G) Ala->Ala. sequence w/ G00101yes
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sbb10 Basic Parts AC1G00101pBjk2741-sbb163/12/2010perfectno
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sbb10 Basic Parts AB2G00101pBjk2741-sbb053/12/2010perfectnoBen BubenheimConfirmed
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sbb10 Basic Parts AC2G00101pBjk2741-sbb053/12/2010not needed
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sbb10 Basic Parts AE2G00101pBjk2741-sbb153/12/2010perfectZhen Zong HuangConfirmed
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sbb10 Basic Parts AF2G00101pBjk2741-ssb083/12/2010PerfectCarolyn Kwok
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sbb10 Basic Parts AG2G00101pBjk2741-Sbb433/12/2010Perfect =DnoDorothy Tulanont
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sbb10 Basic Parts AH2G00101pBjk2741-Bca11523/12/2010good read, not right product, possible too small for no other good clones :(
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sbb10 Basic Parts AA3G00101pBjk2741-sbb173/12/20102 point mutationsJoanna Chen
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sbb10 Basic Parts AB3G00101pBjk2741-sbb173/12/20101 bp deletionJoanna Chen
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sbb10 Basic Parts AC3G00101pBjk2741-sbb183/12/2010perfectnoJoanna Chen
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sbb10 Basic Parts AD3G00101pBjk2741-sbb183/12/2010perfectJoanna Chen
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sbb10 Basic Parts BA3G00101pBjk2741-sbb173/16/2010perfectJoanna Chen
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sbb10 Basic Parts BB3G00101pBjk2741-sbb173/16/2010perfectJoanna Chen
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sbb10 Basic Parts AH1G00101pBca9523-sbb303/16/2010perfect partialseq rev
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sbb10 Basic Parts BD1G00101pBca9523-sbb273/16/2010silent mutation (349bp A->G) Ala->Ala. but all good. :)noconfirmed
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sbb10 Basic Parts AE3G00101pBjk2741-sbb343/16/2010perfectnoJeni Lee
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sbb10 Basic Parts AF3G00101pBca1256-sbb293/16/2010possible deletion at 23 and 174, insertion at 79, and substitution at 152 (low mapping signals for first 200 or so bps)yesJenna Kelleherperfect after modifying model
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sbb10 Basic Parts AG3G00101pBjk2741-sbb143/16/2010garbage
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sbb10 Basic Parts AH3G00101pBjk2741-sbb023/16/2010Perfect Partialneeds ca998 and pot. internal read
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sbb10 Basic Parts AA4G00101pBjk2741-sbb063/16/2010Deletion: ~85 bp
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sbb10 Basic Parts AB4G00101pBjk2741-sbb063/16/2010Deletion: ~85 bp
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sbb10 Basic Parts AC4G00101pBjk2741-sbb333/16/2010missense mutation. bp354 C->T. aa98 R->Kyes, but a different cloneRaffi Hagopianhold for genR test
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sbb10 Basic Parts AD4G00101pBjk2741-sbb093/16/2010Read looks weird for the part around BamHI. Need to resequence this sample.yes, on the same cloneRaffi Hagopianseq 2nd clone
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sbb10 Basic Parts AE4G00101pBjk2741-sbb193/16/2010PerfectnoMaziar Behtash
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sbb10 Basic Parts AF4G00101pBca9145-sbb233/16/2010good read, weird stuff is going onno, part is being redoneMaziar Behtash
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sbb10 Basic Parts AG4G00101pBca1256-sbb283/16/2010doesn't contain entire part - missing first 200 or so bpsyesJenna Kelleherdeletion on terminus, do clone B
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sbb10 Basic Parts AH4G00101pBjk2741-sbb013/16/2010read is somewhere random in the plasmid near the spec resistance, need to resequenceyesBen Bubenheim
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sbb10 Basic Parts AA5G00101pBca1256-sbb253/16/2010point deletion at bp339yesDorothy Tulanont
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sbb10 Basic Parts AB5G00101pBca1256-sbb263/16/201050bp deletion (from 340 to 389)yesDorothy Tulanont
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sbb10 Basic Parts AC5G00101pBjk2741-sbb113/16/2010PerfectnoWeiChunHsu
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sbb10 Basic Parts AD5G00101pBjk2741-sbb133/16/2010Unacceptable read quality. Try another clone regardless.yes, different clone (product too big for just one read anyways)WeiChunHsumixed read, read A-G5
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sbb10 Basic Parts AH1ca998pBca9523-sbb303/19/2010perfect with 600101 read
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sbb10 Basic Parts AH3ca998pBjk2741-sbb023/19/2010read internalall but patch in middle confirmed
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sbb10 Basic Parts AG5G00101pBjk2741-sbb133/19/2010Perfect partial (out to ~900 or so)yes, forward (ca998)WeiChunHsu
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sbb10 Basic Parts AE5G00101pBjk2741-sbb223/19/2010
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sbb10 Basic Parts AA6G00101pBjk2741-sbb143/19/2010No match, redo
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sbb10 Basic Parts AB6G00101pBjk2741-sbb203/19/2010No part
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sbb10 Basic Parts BD1ca998pBca9523-sbb273/19/2010perfect
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sbb10 Basic Parts BD2G00101pBjk2741-sbb313/19/2010
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sbb10 Basic Parts BA4G00101pBjk2741-sbb063/19/2010
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sbb10 Basic Parts BB4G00101pBjk2741-sbb063/19/2010
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sbb10 Basic Parts BD4G00101pBjk2741-sbb093/19/2010<<need to look at trace
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sbb10 Basic Parts BG4G00101pBca1256-sbb283/19/2010read for
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sbb10 Basic Parts BH4G00101pBjk2741-sbb013/19/2010
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sbb10 Basic Parts BA5G00101pBca1256-sbb253/19/2010Both A and B clones identical (a point deletion at base pair 339.), modifying map
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sbb10 Basic Parts BB5G00101pBca1256-sbb263/19/2010maps crazy, modifying, going with clone A (read error over long stretch, but A's matches original model the best
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sbb10 Basic Parts BG4ca998pBca1256-sbb283/20/2010Close enough, modifying mapBG4
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sbb10 Basic Parts AG5ca998pBjk2741-sbb133/20/2010completes phiC31 over 3 reads is 100% correct1AG5
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sbb10 Basic Parts AG5CA1645pBjk2741-sbb133/20/2010phiC312AG5
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sbb10 Basic Parts AH3CA1471pBjk2741-sbb023/20/2010That completes N15, it's 100% correctAH3
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sbb10 Basic Parts AE6G00101pBjk2741-sbb073/20/2010tn5 perfect over 2 readsAE6R
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sbb10 Basic Parts AE6ca998pBjk2741-sbb073/20/2010tn5AE6F
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sbb10 Basic Parts AF6G00101pBjk2741-sbb103/20/2010sb perfect over 2 readsAF6R
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sbb10 Basic Parts AF6ca998pBjk2741-sbb103/20/2010sbAF6F
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sbb10 Basic Parts CA2G00101pBjk2741-sbb063/24/2010perfect
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sbb10 Basic Parts CA3G00101pBjk2741-sbb203/24/2010perfect
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sbb10 Basic Parts CA4G00101pBjk2741-Bca17453/24/2010pt mutation, seq other cloneseq other clone
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sbb10 Basic Parts CA5G00101pBjk2741-sbb043/24/2010pt mutation, seq other clone
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sbb10 Basic Parts CA6G00101pBjk2741-Bca11523/24/2010perfect
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sbb10 Basic Parts CA7G00101pBjk2741-Bjh18583/24/2010perfect
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sbb10 Basic Parts CE1G00101pBca1256-jtk26253/24/2010perfect partial, in wrong vectorca998
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sbb10 Basic Parts CE2G00101pBjk2741-sbb013/24/2010perfect partialca998
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sbb10 Basic Parts CE3G00101pBjk2741-sbb213/24/2010perfect
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sbb10 Basic Parts CE4G00101pBjk2741-sbb143/24/2010perfect partialca998
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sbb10 Basic Parts CE5G00101pBca9523-sbb363/24/2010perfect
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sbb10 Basic Parts CE6G00101pBjk2741-Bjh13803/24/2010perfect
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sbb10 Basic Parts CE7G00101pBjk2741-Bth80623/24/2010perfect
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sbb10 Basic Parts CE8G00101pBca9145-sbb233/24/2010mssing BglII at end of zfn site, otherwise correctseq other clone
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sbb10 Basic Parts CB8G00101pBca9145-sbb033/24/20101 pt del in tos, otherwise correctmap more, seq 2 more
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sbb10 Basic Parts CB4G00101pBjk2741-Bca17453/25/2010errors not in template, seq more
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sbb10 Basic Parts CB5G00101pBjk2741-sbb043/25/2010errors not in template, seq more
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sbb10 Basic Parts CE1ca998pBca1256-jtk26253/25/2010gave a reverse read, need another -- it's in the wrong vector, see xhoi site
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sbb10 Basic Parts CE2ca998pBjk2741-sbb013/25/2010pt mut in forward, seq another
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sbb10 Basic Parts CE4ca998pBjk2741-sbb143/25/2010region around BglII is wrong, but should still work, modifying map
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sbb10 Basic Parts CF8G00101pBca9145-sbb233/25/2010perfect
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sbb10 Basic Parts CC4G00101pBjk2741-Bca1745perfect partial, seq forward
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sbb10 Basic Parts CD4G00101pBjk2741-Bca1745perfect partial, seq forward
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sbb10 Basic Parts CC5G00101pBjk2741-sbb04perfect partial, seq forward
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sbb10 Basic Parts CD5G00101pBjk2741-sbb04perfect partial, seq forward
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sbb10 Basic Parts CE1ca998pBca1256-jtk2625looks to be in pBca9145
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