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1 | plate | well | Oligo | Construct | dataReceived | Analysis | Additional Read Required | User | JCA_Note | ||||||||||||||

2 | sbb10 Basic Parts A | A1 | ca998 | pBjk2741-sbb12 | 3/10/2010 | perfect | ASaarni | Confirmed | |||||||||||||||

3 | sbb10 Basic Parts A | B1 | ca998 | pBjk2741-sbb12 | 3/10/2010 | perfect (possible mutation in EcoR1 site) | ASaarni | dropped | |||||||||||||||

4 | sbb10 Basic Parts A | C1 | ca998 | pBjk2741-sbb16 | 3/10/2010 | perfect | no | Kai Mesa | |||||||||||||||

5 | sbb10 Basic Parts A | D1 | ca998 | pBca9523-sbb27 | 3/10/2010 | possible 1 bp insertion at BglII site, silent mutation in cds(349bp) | yes | ||||||||||||||||

6 | sbb10 Basic Parts A | E1 | ca998 | pBjk2741-sbb42 | 3/10/2010 | perfect (possible mutation in EcoR1 site) | Jenna Kelleher | Confirmed | |||||||||||||||

7 | sbb10 Basic Parts A | F1 | ca998 | pBca9523-sbb24 | 3/10/2010 | perfect partial (1 silent mutation, 1 point mutation) | yes | Carolyn Kwok | Sequencing 2nd clone | ||||||||||||||

8 | sbb10 Basic Parts A | G1 | ca998 | pBjk2741-sbb32 | 3/10/2010 | perfect | Carolyn Kwok | Confirmed | |||||||||||||||

9 | sbb10 Basic Parts A | H1 | ca998 | pBca9523-sbb30 | 3/10/2010 | 2 point mutations (bp:79, 390) | yes | Jose Gutierrez | Sequencing 2nd clone | ||||||||||||||

10 | sbb10 Basic Parts A | A2 | ca998 | pBjk2741-ig114 | 3/10/2010 | perfect partial (2 point mutations) | Eco/Bam transfer doesn't nee reread | Jose Gutierrez | Confirmed | ||||||||||||||

11 | |||||||||||||||||||||||

12 | sbb10 Basic Parts B | F1 | ca998 | pBca9523-sbb24 | 3/12/2010 | 2 point mutations | no | Carolyn Kwok | modify model, keep this clone | ||||||||||||||

13 | sbb10 Basic Parts B | H1 | ca998 | pBca9523-sbb30 | 3/12/2010 | possible deletion at bp 47, also looks like contaminated DNA, also c-t mutation at 126 | yes | Jose Gutierrez | |||||||||||||||

14 | sbb10 Basic Parts B | D1 | ca998 | pBca9523-sbb27 | 3/12/2010 | silent mutation (349bp A->G) Ala->Ala. sequence w/ G00101 | yes | ||||||||||||||||

15 | sbb10 Basic Parts A | C1 | G00101 | pBjk2741-sbb16 | 3/12/2010 | perfect | no | ||||||||||||||||

16 | sbb10 Basic Parts A | B2 | G00101 | pBjk2741-sbb05 | 3/12/2010 | perfect | no | Ben Bubenheim | Confirmed | ||||||||||||||

17 | sbb10 Basic Parts A | C2 | G00101 | pBjk2741-sbb05 | 3/12/2010 | not needed | |||||||||||||||||

18 | sbb10 Basic Parts A | E2 | G00101 | pBjk2741-sbb15 | 3/12/2010 | perfect | Zhen Zong Huang | Confirmed | |||||||||||||||

19 | sbb10 Basic Parts A | F2 | G00101 | pBjk2741-ssb08 | 3/12/2010 | Perfect | Carolyn Kwok | ||||||||||||||||

20 | sbb10 Basic Parts A | G2 | G00101 | pBjk2741-Sbb43 | 3/12/2010 | Perfect =D | no | Dorothy Tulanont | |||||||||||||||

21 | sbb10 Basic Parts A | H2 | G00101 | pBjk2741-Bca1152 | 3/12/2010 | good read, not right product, possible too small for | no other good clones :( | ||||||||||||||||

22 | sbb10 Basic Parts A | A3 | G00101 | pBjk2741-sbb17 | 3/12/2010 | 2 point mutations | Joanna Chen | ||||||||||||||||

23 | sbb10 Basic Parts A | B3 | G00101 | pBjk2741-sbb17 | 3/12/2010 | 1 bp deletion | Joanna Chen | ||||||||||||||||

24 | sbb10 Basic Parts A | C3 | G00101 | pBjk2741-sbb18 | 3/12/2010 | perfect | no | Joanna Chen | |||||||||||||||

25 | sbb10 Basic Parts A | D3 | G00101 | pBjk2741-sbb18 | 3/12/2010 | perfect | Joanna Chen | ||||||||||||||||

26 | |||||||||||||||||||||||

27 | sbb10 Basic Parts B | A3 | G00101 | pBjk2741-sbb17 | 3/16/2010 | perfect | Joanna Chen | ||||||||||||||||

28 | sbb10 Basic Parts B | B3 | G00101 | pBjk2741-sbb17 | 3/16/2010 | perfect | Joanna Chen | ||||||||||||||||

29 | sbb10 Basic Parts A | H1 | G00101 | pBca9523-sbb30 | 3/16/2010 | perfect partial | seq rev | ||||||||||||||||

30 | sbb10 Basic Parts B | D1 | G00101 | pBca9523-sbb27 | 3/16/2010 | silent mutation (349bp A->G) Ala->Ala. but all good. :) | no | confirmed | |||||||||||||||

31 | sbb10 Basic Parts A | E3 | G00101 | pBjk2741-sbb34 | 3/16/2010 | perfect | no | Jeni Lee | |||||||||||||||

32 | sbb10 Basic Parts A | F3 | G00101 | pBca1256-sbb29 | 3/16/2010 | possible deletion at 23 and 174, insertion at 79, and substitution at 152 (low mapping signals for first 200 or so bps) | yes | Jenna Kelleher | perfect after modifying model | ||||||||||||||

33 | sbb10 Basic Parts A | G3 | G00101 | pBjk2741-sbb14 | 3/16/2010 | garbage | |||||||||||||||||

34 | sbb10 Basic Parts A | H3 | G00101 | pBjk2741-sbb02 | 3/16/2010 | Perfect Partial | needs ca998 and pot. internal read | ||||||||||||||||

35 | sbb10 Basic Parts A | A4 | G00101 | pBjk2741-sbb06 | 3/16/2010 | Deletion: ~85 bp | |||||||||||||||||

36 | sbb10 Basic Parts A | B4 | G00101 | pBjk2741-sbb06 | 3/16/2010 | Deletion: ~85 bp | |||||||||||||||||

37 | sbb10 Basic Parts A | C4 | G00101 | pBjk2741-sbb33 | 3/16/2010 | missense mutation. bp354 C->T. aa98 R->K | yes, but a different clone | Raffi Hagopian | hold for genR test | ||||||||||||||

38 | sbb10 Basic Parts A | D4 | G00101 | pBjk2741-sbb09 | 3/16/2010 | Read looks weird for the part around BamHI. Need to resequence this sample. | yes, on the same clone | Raffi Hagopian | seq 2nd clone | ||||||||||||||

39 | sbb10 Basic Parts A | E4 | G00101 | pBjk2741-sbb19 | 3/16/2010 | Perfect | no | Maziar Behtash | |||||||||||||||

40 | sbb10 Basic Parts A | F4 | G00101 | pBca9145-sbb23 | 3/16/2010 | good read, weird stuff is going on | no, part is being redone | Maziar Behtash | |||||||||||||||

41 | sbb10 Basic Parts A | G4 | G00101 | pBca1256-sbb28 | 3/16/2010 | doesn't contain entire part - missing first 200 or so bps | yes | Jenna Kelleher | deletion on terminus, do clone B | ||||||||||||||

42 | sbb10 Basic Parts A | H4 | G00101 | pBjk2741-sbb01 | 3/16/2010 | read is somewhere random in the plasmid near the spec resistance, need to resequence | yes | Ben Bubenheim | |||||||||||||||

43 | sbb10 Basic Parts A | A5 | G00101 | pBca1256-sbb25 | 3/16/2010 | point deletion at bp339 | yes | Dorothy Tulanont | |||||||||||||||

44 | sbb10 Basic Parts A | B5 | G00101 | pBca1256-sbb26 | 3/16/2010 | 50bp deletion (from 340 to 389) | yes | Dorothy Tulanont | |||||||||||||||

45 | sbb10 Basic Parts A | C5 | G00101 | pBjk2741-sbb11 | 3/16/2010 | Perfect | no | WeiChunHsu | |||||||||||||||

46 | sbb10 Basic Parts A | D5 | G00101 | pBjk2741-sbb13 | 3/16/2010 | Unacceptable read quality. Try another clone regardless. | yes, different clone (product too big for just one read anyways) | WeiChunHsu | mixed read, read A-G5 | ||||||||||||||

47 | |||||||||||||||||||||||

48 | sbb10 Basic Parts A | H1 | ca998 | pBca9523-sbb30 | 3/19/2010 | perfect with 600101 read | |||||||||||||||||

49 | sbb10 Basic Parts A | H3 | ca998 | pBjk2741-sbb02 | 3/19/2010 | read internal | all but patch in middle confirmed | ||||||||||||||||

50 | sbb10 Basic Parts A | G5 | G00101 | pBjk2741-sbb13 | 3/19/2010 | Perfect partial (out to ~900 or so) | yes, forward (ca998) | WeiChunHsu | |||||||||||||||

51 | sbb10 Basic Parts A | E5 | G00101 | pBjk2741-sbb22 | 3/19/2010 | ||||||||||||||||||

52 | sbb10 Basic Parts A | A6 | G00101 | pBjk2741-sbb14 | 3/19/2010 | No match, redo | |||||||||||||||||

53 | sbb10 Basic Parts A | B6 | G00101 | pBjk2741-sbb20 | 3/19/2010 | No part | |||||||||||||||||

54 | sbb10 Basic Parts B | D1 | ca998 | pBca9523-sbb27 | 3/19/2010 | perfect | |||||||||||||||||

55 | sbb10 Basic Parts B | D2 | G00101 | pBjk2741-sbb31 | 3/19/2010 | ||||||||||||||||||

56 | sbb10 Basic Parts B | A4 | G00101 | pBjk2741-sbb06 | 3/19/2010 | ||||||||||||||||||

57 | sbb10 Basic Parts B | B4 | G00101 | pBjk2741-sbb06 | 3/19/2010 | ||||||||||||||||||

58 | sbb10 Basic Parts B | D4 | G00101 | pBjk2741-sbb09 | 3/19/2010 | <<need to look at trace | |||||||||||||||||

59 | sbb10 Basic Parts B | G4 | G00101 | pBca1256-sbb28 | 3/19/2010 | read for | |||||||||||||||||

60 | sbb10 Basic Parts B | H4 | G00101 | pBjk2741-sbb01 | 3/19/2010 | ||||||||||||||||||

61 | sbb10 Basic Parts B | A5 | G00101 | pBca1256-sbb25 | 3/19/2010 | Both A and B clones identical (a point deletion at base pair 339.), modifying map | |||||||||||||||||

62 | sbb10 Basic Parts B | B5 | G00101 | pBca1256-sbb26 | 3/19/2010 | maps crazy, modifying, going with clone A (read error over long stretch, but A's matches original model the best | |||||||||||||||||

63 | |||||||||||||||||||||||

64 | sbb10 Basic Parts B | G4 | ca998 | pBca1256-sbb28 | 3/20/2010 | Close enough, modifying map | BG4 | ||||||||||||||||

65 | sbb10 Basic Parts A | G5 | ca998 | pBjk2741-sbb13 | 3/20/2010 | completes phiC31 over 3 reads is 100% correct | 1AG5 | ||||||||||||||||

66 | sbb10 Basic Parts A | G5 | CA1645 | pBjk2741-sbb13 | 3/20/2010 | phiC31 | 2AG5 | ||||||||||||||||

67 | sbb10 Basic Parts A | H3 | CA1471 | pBjk2741-sbb02 | 3/20/2010 | That completes N15, it's 100% correct | AH3 | ||||||||||||||||

68 | sbb10 Basic Parts A | E6 | G00101 | pBjk2741-sbb07 | 3/20/2010 | tn5 perfect over 2 reads | AE6R | ||||||||||||||||

69 | sbb10 Basic Parts A | E6 | ca998 | pBjk2741-sbb07 | 3/20/2010 | tn5 | AE6F | ||||||||||||||||

70 | sbb10 Basic Parts A | F6 | G00101 | pBjk2741-sbb10 | 3/20/2010 | sb perfect over 2 reads | AF6R | ||||||||||||||||

71 | sbb10 Basic Parts A | F6 | ca998 | pBjk2741-sbb10 | 3/20/2010 | sb | AF6F | ||||||||||||||||

72 | |||||||||||||||||||||||

73 | sbb10 Basic Parts C | A2 | G00101 | pBjk2741-sbb06 | 3/24/2010 | perfect | |||||||||||||||||

74 | sbb10 Basic Parts C | A3 | G00101 | pBjk2741-sbb20 | 3/24/2010 | perfect | |||||||||||||||||

75 | sbb10 Basic Parts C | A4 | G00101 | pBjk2741-Bca1745 | 3/24/2010 | pt mutation, seq other clone | seq other clone | ||||||||||||||||

76 | sbb10 Basic Parts C | A5 | G00101 | pBjk2741-sbb04 | 3/24/2010 | pt mutation, seq other clone | |||||||||||||||||

77 | sbb10 Basic Parts C | A6 | G00101 | pBjk2741-Bca1152 | 3/24/2010 | perfect | |||||||||||||||||

78 | sbb10 Basic Parts C | A7 | G00101 | pBjk2741-Bjh1858 | 3/24/2010 | perfect | |||||||||||||||||

79 | sbb10 Basic Parts C | E1 | G00101 | pBca1256-jtk2625 | 3/24/2010 | perfect partial, in wrong vector | ca998 | ||||||||||||||||

80 | sbb10 Basic Parts C | E2 | G00101 | pBjk2741-sbb01 | 3/24/2010 | perfect partial | ca998 | ||||||||||||||||

81 | sbb10 Basic Parts C | E3 | G00101 | pBjk2741-sbb21 | 3/24/2010 | perfect | |||||||||||||||||

82 | sbb10 Basic Parts C | E4 | G00101 | pBjk2741-sbb14 | 3/24/2010 | perfect partial | ca998 | ||||||||||||||||

83 | sbb10 Basic Parts C | E5 | G00101 | pBca9523-sbb36 | 3/24/2010 | perfect | |||||||||||||||||

84 | sbb10 Basic Parts C | E6 | G00101 | pBjk2741-Bjh1380 | 3/24/2010 | perfect | |||||||||||||||||

85 | sbb10 Basic Parts C | E7 | G00101 | pBjk2741-Bth8062 | 3/24/2010 | perfect | |||||||||||||||||

86 | sbb10 Basic Parts C | E8 | G00101 | pBca9145-sbb23 | 3/24/2010 | mssing BglII at end of zfn site, otherwise correct | seq other clone | ||||||||||||||||

87 | sbb10 Basic Parts C | B8 | G00101 | pBca9145-sbb03 | 3/24/2010 | 1 pt del in tos, otherwise correct | map more, seq 2 more | ||||||||||||||||

88 | |||||||||||||||||||||||

89 | sbb10 Basic Parts C | B4 | G00101 | pBjk2741-Bca1745 | 3/25/2010 | errors not in template, seq more | |||||||||||||||||

90 | sbb10 Basic Parts C | B5 | G00101 | pBjk2741-sbb04 | 3/25/2010 | errors not in template, seq more | |||||||||||||||||

91 | sbb10 Basic Parts C | E1 | ca998 | pBca1256-jtk2625 | 3/25/2010 | gave a reverse read, need another -- it's in the wrong vector, see xhoi site | |||||||||||||||||

92 | sbb10 Basic Parts C | E2 | ca998 | pBjk2741-sbb01 | 3/25/2010 | pt mut in forward, seq another | |||||||||||||||||

93 | sbb10 Basic Parts C | E4 | ca998 | pBjk2741-sbb14 | 3/25/2010 | region around BglII is wrong, but should still work, modifying map | |||||||||||||||||

94 | sbb10 Basic Parts C | F8 | G00101 | pBca9145-sbb23 | 3/25/2010 | perfect | |||||||||||||||||

95 | |||||||||||||||||||||||

96 | sbb10 Basic Parts C | C4 | G00101 | pBjk2741-Bca1745 | perfect partial, seq forward | ||||||||||||||||||

97 | sbb10 Basic Parts C | D4 | G00101 | pBjk2741-Bca1745 | perfect partial, seq forward | ||||||||||||||||||

98 | sbb10 Basic Parts C | C5 | G00101 | pBjk2741-sbb04 | perfect partial, seq forward | ||||||||||||||||||

99 | sbb10 Basic Parts C | D5 | G00101 | pBjk2741-sbb04 | perfect partial, seq forward | ||||||||||||||||||

100 | sbb10 Basic Parts C | E1 | ca998 | pBca1256-jtk2625 | looks to be in pBca9145 |

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